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9uu4

Cryo-EM structure of the maize CER6-GL2 complex (inactive C222A mutant) bound with malonyl-CoA

Method: ELECTRON MICROSCOPY D_max: 139.3 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9uu4
deposition_date	2025-05-05
title	Cryo-EM structure of the maize CER6-GL2 complex (inactive C222A mutant) bound with malonyl-CoA
keywords	Complex, inactive C222A mutant, Malonyl-CoA, PLANT PROTEIN; PLANT PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	43.18 Å
rg_electron	43.03 Å
i0	518052000.00
molecular_weight	186320.0 kDa
excluded_volume	232980 Å³
envelope_volume	310660 Å³
shell_volume	60139 Å³
envelope_diameter	143.4 Å
shell_rg	47.56 Å
envelope_rg	43.32 Å
shape_rg	43.05 Å
total_rg	43.16 Å
total_atoms	13094
n_residues	1676
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	139.3 Å
rg_real	43.17 Å
rg_real_error	0.94 Å
i0_real	5.1810e+08
i0_real_error	8.1510e+06
rg_reciprocal	43.18 Å
i0_reciprocal	518100000.0000
total_estimate	0.8954
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	41.4 Å
skewness	0.210
kurtosis	-0.747
angular_range	— – 0.1850 Å⁻¹
current_alpha	0.0001
highest_alpha	149700000.0000
n_real_points	38
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.919; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.983; Smooth: 0.897

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	Protein ECERIFERUM 26-like
formula_weight	46865.0 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,D
atom_count	13094

DYKDDDDKMVFEQHEEEAVAPGAVHGHRLSTVVPSSVTGEVDYALADADLAFKLHYLRGVYYYRSGDGLATKVLKDPMFPWLDDHFPVAGRVRRAEAEGDGAPRRPYIKCNDCGVRIVEARCDRDMAEWIRDAAPGRIRQLCYDKVLGPELFFSPLLYVQITNFKCGGLALGFSWAHLIGDIPSAATCFNKWAQILSGKKPEATVLTPPNQPLQGQSPAAPRSVKQVGPMEDLWLVPAGRDMACYSFHVSDAVLKKLHQQQNGRQDAAAGTFELVSALVWQAVAKIRGDVDTVTVVRADAAARSGKSLANEMKVGYVESAGSSPAKTDVAELAALLAKNVVDETAAVAAFQGDVLVYGGANLTLVDMEQVDLYGLEIKGQRPVYVEYGMDGVGDEGAVLVQPDADGRGRLVTVVLPGDEIDSLRAALGSALHVA

Source

organism	Zea mays
common_name	Maize
ncbi_taxonomy_id	4577
host_organism	Spodoptera frugiperda

Entity 2 polymer

description	3-ketoacyl-CoA synthase
formula_weight	56381.4 kDa
ec_number	2.3.1.-
mutation	C222A
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	C,B

HHHHHHMPSGGVFSGSVNLKHVKLGYQYLVNHFLTLLLVPVMAATALELARLGPGELLSLWRSLELDLVHILCSAFLVVFVGTVYVMSRPRPVYLVDYACYKPPASCRVPFATFMEHTRLISDDDKSVRFQTRILERSGLGEDTCLPPANHYIPPNPSMEASRAEAQLVIFSAIDDLVRRTGLKPKDIDILVVNCSLFSPTPSLSAMIINKYKLRSNIRSFNLSGMGASAGLISIDLARDMLQVHPNSNALVVSTEIITPNFYQGSRRDMLLPNCLFRMGAAAILLSNRRREARRAKYRLVHVVRTHKGADDRAYRCVYEEEDEQGFSGISLSKELMAIAGDALKSNITTIGPLVLPMSEQLLFFFRLVGRKLVNKGWRPYIPDFKLAFEHFCIHAGGRAVIDELQKNLQLSPRHVEASRMTLHRFGNTSSSSLWYELAYIEAKGRMRRGDRVWQIGFGSGFKCNSAVWKCLRSIKTPTNGPWDDCIHRYPVDVPEVVKL

Source

organism	Zea mays
common_name	Maize
ncbi_taxonomy_id	4577
host_organism	Spodoptera frugiperda

Entity 3 non-polymer

description	MALONYL-COENZYME A
formula_weight	853.6 kDa
src_method	syn
entity_count	2

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.96 Å
resolution_method	—
num_particles	156994

fit	/app/data/saxs_computation/uu/9uu4/9uu4.fit
pddf	/app/data/pr_computation/uu/9uu4/9uu4.pr.out
profile	/app/data/saxs_computation/uu/9uu4/9uu4.dat

Cryo-EM structure of the maize CER6-GL2 complex (inactive C222A mutant) bound with malonyl-CoA

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)