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9uuy

Wild-type apo Escherichia coli nicotinamide nucleotide transhydrogenase

Method: ELECTRON MICROSCOPY D_max: 103.2 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9uuy
deposition_date	2025-05-08
title	Wild-type apo Escherichia coli nicotinamide nucleotide transhydrogenase
keywords	;Nicotinamide nucleotide transhydrogenase, Mitochondrial inner membrane, E. coli transhydrogenase, Hydride transfer, Proton motive force, NAD+, NADH, NADP+, NADPH, MEMBRANE PROTEIN ;; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	33.29 Å
rg_electron	32.46 Å
i0	176278000.00
molecular_weight	114330.0 kDa
excluded_volume	146650 Å³
envelope_volume	183950 Å³
shell_volume	45918 Å³
envelope_diameter	103.0 Å
shell_rg	40.57 Å
envelope_rg	32.18 Å
shape_rg	32.45 Å
total_rg	33.15 Å
total_atoms	8042
n_residues	1074
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	103.2 Å
rg_real	33.11 Å
rg_real_error	0.74 Å
i0_real	1.7630e+08
i0_real_error	2.9020e+06
rg_reciprocal	33.19 Å
i0_reciprocal	176300000.0000
total_estimate	0.9091
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	1
r_peak_primary	43.0 Å
skewness	0.121
kurtosis	-0.631
angular_range	— – 0.2400 Å⁻¹
current_alpha	0.0000
highest_alpha	36100000.0000
n_real_points	49
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.956; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.947

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	NAD(P) transhydrogenase subunit beta
formula_weight	48762.7 kDa
ec_number	7.1.1.1
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	B,A
atom_count	8042

MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIALIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVGFNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNRHKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFGTDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLRARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGANDTVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGDAKASVDAILKAL

Source

organism	Escherichia coli K-12
ncbi_taxonomy_id	83333
host_organism	Escherichia coli

Entity 2 polymer

description	NAD(P) transhydrogenase subunit alpha
formula_weight	10742.8 kDa
ec_number	7.1.1.1
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	C,D

ILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFKN

Source

organism	Escherichia coli K-12
ncbi_taxonomy_id	83333
host_organism	Escherichia coli

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.85 Å
resolution_method	—
num_particles	204263

fit	/app/data/saxs_computation/uu/9uuy/9uuy.fit
pddf	/app/data/pr_computation/uu/9uuy/9uuy.pr.out
profile	/app/data/saxs_computation/uu/9uuy/9uuy.dat

Wild-type apo Escherichia coli nicotinamide nucleotide transhydrogenase

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)