Cryo-EM structure of the human P2X2 receptor in the ATP-bound desensitised state

Method: ELECTRON MICROSCOPY D_max: 108.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9uwy
deposition_date	2025-05-13
title	Cryo-EM structure of the human P2X2 receptor in the ATP-bound desensitised state
keywords	P2X ion channal, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	33.32 Å
rg_electron	33.37 Å
i0	184168000.00
molecular_weight	109790.0 kDa
excluded_volume	137910 Å³
envelope_volume	187040 Å³
shell_volume	47787 Å³
envelope_diameter	117.5 Å
shell_rg	39.26 Å
envelope_rg	33.53 Å
shape_rg	33.34 Å
total_rg	33.96 Å
total_atoms	7725
n_residues	966
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	108.1 Å
rg_real	33.36 Å
rg_real_error	0.83 Å
i0_real	1.8420e+08
i0_real_error	3.1270e+06
rg_reciprocal	33.35 Å
i0_reciprocal	184200000.0000
total_estimate	0.8581
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	38.3 Å
skewness	0.463
kurtosis	-0.016
angular_range	— – 0.2400 Å⁻¹
current_alpha	0.0000
highest_alpha	25580000.0000
n_real_points	49
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.841; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.629

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	P2X purinoceptor 2
formula_weight	51815.0 kDa
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	A,B,C
atom_count	7725

MAAAQPKYPAGATARRLARGCWSALWDYETPKVIVVRNRRLGVLYRAVQLLILLYFVWYVFIVQKSYQESETGPESSIITKVKGITTSEHKVWDVEEYVKPPEGGSVFSIITRVEATHSQTQGTCPESIRVHNATCLSDADCVAGELDMLGNGLRTGRCVPYYQGPSKTCEVFGWCPVEDGASVSQFLGTMAPNFTILIKNSIHYPKFHFSKGNIADRTDGYLKRCTFHEASDLYCPIFKLGFIVEKAGESFTELAHKGGVIGVIINWDCDLDLPASECNPKYSFRRLDPKHVPASSGYNFRFAKYYKINGTTTRTLIKAYGIRIDVIVHGQAGKFSLIPTIINLATALTSVGVGSFLCDWILLTFMNKNKVYSHKKFDKVCTPSHPSGSWPVTLARVLGQAPPEPGHRSEDQHPSPPSGQEGQQGAECGPAFPPLRPCPISAPSEQMVDTPASEPAQASTPTDPKGLAQL

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 2 non-polymer

description	2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	221.2 kDa
src_method	syn
entity_count	6

Entity 3 non-polymer

description	ADENOSINE-5'-TRIPHOSPHATE
formula_weight	507.2 kDa
src_method	syn
entity_count	3

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6. Citations (1)

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resolution	2.48 Å
resolution_method	—
num_particles	112552

fit	/app/data/saxs_computation/uw/9uwy/9uwy.fit
pddf	/app/data/pr_computation/uw/9uwy/9uwy.pr.out
profile	/app/data/saxs_computation/uw/9uwy/9uwy.dat