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9vbo

;Cryo-EM structure of the multi-component acyltransferase complex MucABC from Streptococcus macacae at a stoichiometric ratio of 4:2:2 ;

Method: ELECTRON MICROSCOPY D_max: 119.7 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9vbo
deposition_date	2025-06-04
title	;Cryo-EM structure of the multi-component acyltransferase complex MucABC from Streptococcus macacae at a stoichiometric ratio of 4:2:2 ;
keywords	enzyme complex, thiolase, Friedel-Crafts acylation, biosynthesis, TRANSFERASE; TRANSFERASE
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	39.21 Å
rg_electron	38.61 Å
i0	1061140000.00
molecular_weight	272370.0 kDa
excluded_volume	342320 Å³
envelope_volume	399250 Å³
shell_volume	80405 Å³
envelope_diameter	119.3 Å
shell_rg	48.21 Å
envelope_rg	38.64 Å
shape_rg	38.59 Å
total_rg	39.12 Å
total_atoms	19167
n_residues	2464
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	119.7 Å
rg_real	38.90 Å
rg_real_error	0.63 Å
i0_real	1.0610e+09
i0_real_error	1.7070e+07
rg_reciprocal	39.10 Å
i0_reciprocal	1061000000.0000
total_estimate	0.9029
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	1
r_peak_primary	50.9 Å
skewness	0.085
kurtosis	-0.595
angular_range	— – 0.2000 Å⁻¹
current_alpha	0.0000
highest_alpha	544100000.0000
n_real_points	41
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.937; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.974; Smooth: 0.948

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	3-oxoacyl-[acyl-carrier-protein (ACP)] synthase III, C-terminal domain protein
formula_weight	39906.0 kDa
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	A,B,C,D
atom_count	19167

MGNKQIGIKSYGISIPYFRLPVEETIKVWNNNNVDYIKNKIGVKRRTVVSSDEDTLTLAMEAGQEAVLHFKEDVAKIDSILLGSCTTPDIFKSNANQLMSFLFNKNDYFGCDIRASENSGAASLVLGYSLVSSGLSNTSLIFSADTLSKNIFPSELREPYIGSGAASIILGKGEDILAEIIGIGNSNASFPEQGRTEDNRYLRVLANLNYSVVKEGRIKRSLESINNALENASLKAEDIKYFVFQDGTEQTYKEFSHFFHFDNVINQDIFKNLGYIGSASPIISMLAALENAEVGDIILMCGYGHSSGSTTVIFRVTEEITFKNKIIDKLKNYKDINYSEAMKHEFKYSQPEISLGTFI

Source

organism	Streptococcus macacae NCTC 11558
ncbi_taxonomy_id	764298
host_organism	Escherichia coli

Entity 2 polymer

description	2,4-diacetylphloroglucinol biosynthesis protein PhlB family protein
formula_weight	16954.5 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	H,G

MFSNEQISNPTIESSLKDWREQGGLTRLEGSKCPHCDELFYPRRFVCPYCFCRSLKTYKFSGMGKIKNIEINSISQVAVIGYREISPRYLSVIELAEGVDVLGEIIECSEIESIHSLIGREVMSVVRKQSRSGNTSWKYGYKFKLKEG

Source

organism	Streptococcus macacae NCTC 11558
ncbi_taxonomy_id	764298
host_organism	Escherichia coli

Entity 3 polymer

description	3-oxoacyl-[acyl-carrier-protein (ACP)] synthase III domain protein
formula_weight	44574.5 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	E,F

MGIKIQVSGVGCTNAPGLPHINKSFKELLVEAAYKALEDAFLSPHLIDGASFSYAGEGEIGHGGIVPTLVDALGLAPLEGYINIGNCASSHMALLQGCEMIESGRYRHVLVAGFDKMTDILPFENYMLMSTDSLYDYNLGFSHIDAFLLQQEYISKYGIQPIKLKEALLKFSTLMKKYGAVNKVSSNFGKELPTSKELENQPFFGNAMSAGEGASAVILSAMEANNKNSSQEKVIIAGRGYTNTSHYIPHRYKEKLLHHKNKDSNDEVGMFNGIPLELSINQAYSEAKISAKDLNILELYDQGLNSFISMEAAGICPKGEAIEYICNGGGTIDSSVAINTDGGNIARGHAGGGASLYQIIEIVKQLQGRASGMQIKKRQYGLSTVIGGAYATAAAIVLKNEEYLEHHHHHH

Source

organism	Streptococcus macacae NCTC 11558
ncbi_taxonomy_id	764298
host_organism	Escherichia coli

Entity 4 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	2

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6. Citations (1)

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resolution	2.16 Å
resolution_method	—
num_particles	1155067

fit	/app/data/saxs_computation/vb/9vbo/9vbo.fit
pddf	/app/data/pr_computation/vb/9vbo/9vbo.pr.out
profile	/app/data/saxs_computation/vb/9vbo/9vbo.dat

;Cryo-EM structure of the multi-component acyltransferase complex MucABC from Streptococcus macacae at a stoichiometric ratio of 4:2:2 ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

EM 3D

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)