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9vbt

;Cryo-EM structure of the multi-component acyltransferase complex MucABC from Streptococcus macacae at a stoichiometric ratio of 4:4:4 ;

Method: ELECTRON MICROSCOPY D_max: 144.6 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9vbt
deposition_date	2025-06-04
title	;Cryo-EM structure of the multi-component acyltransferase complex MucABC from Streptococcus macacae at a stoichiometric ratio of 4:4:4 ;
keywords	enzyme complex, thiolase, Friedel-Crafts acylation, biosynthesis, TRANSFERASE; TRANSFERASE
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	44.17 Å
rg_electron	43.82 Å
i0	2048790000.00
molecular_weight	383120.0 kDa
excluded_volume	481700 Å³
envelope_volume	566420 Å³
shell_volume	100760 Å³
envelope_diameter	146.1 Å
shell_rg	53.33 Å
envelope_rg	43.71 Å
shape_rg	43.77 Å
total_rg	44.29 Å
total_atoms	26945
n_residues	3478
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	144.6 Å
rg_real	44.00 Å
rg_real_error	0.97 Å
i0_real	2.0490e+09
i0_real_error	3.4010e+07
rg_reciprocal	44.17 Å
i0_reciprocal	2049000000.0000
total_estimate	0.6790
solution_quality	REASONABLE a REASONABLE solution
n_peaks	1
r_peak_primary	56.2 Å
skewness	0.263
kurtosis	-0.337
angular_range	— – 0.1800 Å⁻¹
current_alpha	0.0000
highest_alpha	691100000.0000
n_real_points	37
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.844; Stabil: 1.000; Sysdev: 0.145; Positv: 1.000; Valcen: 0.988; Smooth: 0.868

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	3-oxoacyl-[acyl-carrier-protein (ACP)] synthase III, C-terminal domain protein
formula_weight	39906.0 kDa
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	A,B,C,D
atom_count	26945

MGNKQIGIKSYGISIPYFRLPVEETIKVWNNNNVDYIKNKIGVKRRTVVSSDEDTLTLAMEAGQEAVLHFKEDVAKIDSILLGSCTTPDIFKSNANQLMSFLFNKNDYFGCDIRASENSGAASLVLGYSLVSSGLSNTSLIFSADTLSKNIFPSELREPYIGSGAASIILGKGEDILAEIIGIGNSNASFPEQGRTEDNRYLRVLANLNYSVVKEGRIKRSLESINNALENASLKAEDIKYFVFQDGTEQTYKEFSHFFHFDNVINQDIFKNLGYIGSASPIISMLAALENAEVGDIILMCGYGHSSGSTTVIFRVTEEITFKNKIIDKLKNYKDINYSEAMKHEFKYSQPEISLGTFI

Source

organism	Streptococcus macacae NCTC 11558
ncbi_taxonomy_id	764298
host_organism	Escherichia coli

Entity 2 polymer

description	2,4-diacetylphloroglucinol biosynthesis protein PhlB family protein
formula_weight	16954.5 kDa
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	H,K,L,G

MFSNEQISNPTIESSLKDWREQGGLTRLEGSKCPHCDELFYPRRFVCPYCFCRSLKTYKFSGMGKIKNIEINSISQVAVIGYREISPRYLSVIELAEGVDVLGEIIECSEIESIHSLIGREVMSVVRKQSRSGNTSWKYGYKFKLKEG

Source

organism	Streptococcus macacae NCTC 11558
ncbi_taxonomy_id	764298
host_organism	Escherichia coli

Entity 3 polymer

description	3-oxoacyl-[acyl-carrier-protein (ACP)] synthase III domain protein
formula_weight	44574.5 kDa
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	I,J,E,F

MGIKIQVSGVGCTNAPGLPHINKSFKELLVEAAYKALEDAFLSPHLIDGASFSYAGEGEIGHGGIVPTLVDALGLAPLEGYINIGNCASSHMALLQGCEMIESGRYRHVLVAGFDKMTDILPFENYMLMSTDSLYDYNLGFSHIDAFLLQQEYISKYGIQPIKLKEALLKFSTLMKKYGAVNKVSSNFGKELPTSKELENQPFFGNAMSAGEGASAVILSAMEANNKNSSQEKVIIAGRGYTNTSHYIPHRYKEKLLHHKNKDSNDEVGMFNGIPLELSINQAYSEAKISAKDLNILELYDQGLNSFISMEAAGICPKGEAIEYICNGGGTIDSSVAINTDGGNIARGHAGGGASLYQIIEIVKQLQGRASGMQIKKRQYGLSTVIGGAYATAAAIVLKNEEYLEHHHHHH

Source

organism	Streptococcus macacae NCTC 11558
ncbi_taxonomy_id	764298
host_organism	Escherichia coli

Entity 4 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	4

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6. Citations (1)

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resolution	2.32 Å
resolution_method	—
num_particles	71520

fit	/app/data/saxs_computation/vb/9vbt/9vbt.fit
pddf	/app/data/pr_computation/vb/9vbt/9vbt.pr.out
profile	/app/data/saxs_computation/vb/9vbt/9vbt.dat

;Cryo-EM structure of the multi-component acyltransferase complex MucABC from Streptococcus macacae at a stoichiometric ratio of 4:4:4 ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

EM 3D

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)