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9w50

MprF from Pseudomonas aeruginosa mutant- H386C/F389C in nanodisc, C2 symmetry

Method: ELECTRON MICROSCOPY D_max: 132.3 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9w50
deposition_date	2025-08-01
title	MprF from Pseudomonas aeruginosa mutant- H386C/F389C in nanodisc, C2 symmetry
keywords	Multiple peptide resistance factor, Membrane Enzyme, Synthase, Flippase, aminoacylated lipid, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	42.03 Å
rg_electron	41.56 Å
i0	394055000.00
molecular_weight	177120.0 kDa
excluded_volume	228210 Å³
envelope_volume	313790 Å³
shell_volume	62219 Å³
envelope_diameter	136.7 Å
shell_rg	47.05 Å
envelope_rg	41.31 Å
shape_rg	41.57 Å
total_rg	41.81 Å
total_atoms	12502
n_residues	1604
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	132.3 Å
rg_real	41.97 Å
rg_real_error	1.03 Å
i0_real	3.9410e+08
i0_real_error	6.4380e+06
rg_reciprocal	42.03 Å
i0_reciprocal	394100000.0000
total_estimate	0.8835
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	52.2 Å
skewness	0.244
kurtosis	-0.506
angular_range	— – 0.1900 Å⁻¹
current_alpha	0.0000
highest_alpha	44600000.0000
n_real_points	39
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.957; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.613

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	Phosphatidylglycerol lysyltransferase
formula_weight	98251.4 kDa
ec_number	2.3.2.3
mutation	H386C/F389C
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	12502

MWSHPQFEKGGSGMRTDAPVPEHPAPPSSPASPQRIRLIDRITAYRQPIGLVFTLLLFGLALVACYHLLREIDPGALHDAIADVPRPALLGALSATALGFVILLGYEWSASRFAGVTLPMRSLATGGFSAFAIGNAVGLSLLSGGSVRYRLYSRHGIGAAEIARMTLFASLSLGCALPVLAALAALCDLDDAASALHLPRALVAVIAIAVLSLAVGLVAFLARHRLPGERPSPDSLLVRLGRRSLRLPGLRLSLLQLLITALDVAAAATVLYLLLPETPPFAAFLLVYLLALAAGVLSHVPGGVGVFEAVLLAAFAGQLGAAPLAAALLLYRLIYVVLPLLLACLLLLFLEARRLWVTRQAIRVASGFAAPILAILVFLSGVVLLFSGATPAIDTRLECLGCLIPHRLIDASHLVASLIGVLCLLLAQGLRRRLSAAWALTLVLLLVGALLSLLKGFDWEEASLLSLTAALLAMFRRSFYRPSRLMEVPFSPLYVGASICVVGASVWLLLFANQDVHYSNQLWWQFALDADAPRALRAALGSCLLLLALALGWLLRAAPPAIREPNAEELQRAARIIRHSDQPDGGLALTGDKALLFHESDDAFLMYARRGRSMIALYDPIGPAMQRAELIWQFRDLCDLHHARPVFYQVRAENLPFYMDIGLTALKLGEEARVDLLRFDLENKGKEMKDLRYTWNRGQRDGLALEFHEPGQAPLDELKAISDAWLGGKQVREKGFSLGRFTPAYLNFFRIAIVRHQGKPVAFANLLETDSRELASLDLMRVHPDAPKLTMEFLMLGLILHYKAQGHARFSLGMVPLAGLQPRRGAPLTQRLGALVFRRGEQFYNFQGLRRFKDKFQPDWEPRYLAVPAGLDPLVALADTAALIAGGLTGLVKRRNSSSVDAL

Source

organism	Pseudomonas aeruginosa PAO1
ncbi_taxonomy_id	208964
host_organism	Escherichia coli

Entity 2 non-polymer

description	(1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE
formula_weight	751.0 kDa
src_method	syn
entity_count	4

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.300 Å
resolution_low	128.400 Å
r_factor	0.3408
r_work	0.3408
r_free	—

resolution	3.30 Å
resolution_method	—
num_particles	104195

fit	/app/data/saxs_computation/w5/9w50/9w50.fit
pddf	/app/data/pr_computation/w5/9w50/9w50.pr.out
profile	/app/data/saxs_computation/w5/9w50/9w50.dat

MprF from Pseudomonas aeruginosa mutant- H386C/F389C in nanodisc, C2 symmetry

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)