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9w51

MprF from Pseudomonas aeruginosa mutant, H566C in nanodisc, C2 symmetry

Method: ELECTRON MICROSCOPY D_max: 130.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9w51
deposition_date	2025-08-01
title	MprF from Pseudomonas aeruginosa mutant, H566C in nanodisc, C2 symmetry
keywords	Multiple peptide resistance factor, Membrane Enzyme, Synthase, Flippase, aminoacylated lipid, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	41.07 Å
rg_electron	40.68 Å
i0	388563000.00
molecular_weight	174600.0 kDa
excluded_volume	224540 Å³
envelope_volume	307780 Å³
shell_volume	62410 Å³
envelope_diameter	132.4 Å
shell_rg	46.28 Å
envelope_rg	40.56 Å
shape_rg	40.69 Å
total_rg	40.95 Å
total_atoms	12336
n_residues	1604
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	130.5 Å
rg_real	41.03 Å
rg_real_error	1.25 Å
i0_real	3.8860e+08
i0_real_error	7.4870e+06
rg_reciprocal	41.07 Å
i0_reciprocal	388600000.0000
total_estimate	0.8815
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	48.1 Å
skewness	0.272
kurtosis	-0.508
angular_range	— – 0.1900 Å⁻¹
current_alpha	0.0000
highest_alpha	52980000.0000
n_real_points	39
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.943; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.630

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	Phosphatidylglycerol lysyltransferase
formula_weight	98295.4 kDa
ec_number	2.3.2.3
mutation	H566C
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	12370

MWSHPQFEKGGSGMRTDAPVPEHPAPPSSPASPQRIRLIDRITAYRQPIGLVFTLLLFGLALVACYHLLREIDPGALHDAIADVPRPALLGALSATALGFVILLGYEWSASRFAGVTLPMRSLATGGFSAFAIGNAVGLSLLSGGSVRYRLYSRHGIGAAEIARMTLFASLSLGCALPVLAALAALCDLDDAASALHLPRALVAVIAIAVLSLAVGLVAFLARHRLPGERPSPDSLLVRLGRRSLRLPGLRLSLLQLLITALDVAAAATVLYLLLPETPPFAAFLLVYLLALAAGVLSHVPGGVGVFEAVLLAAFAGQLGAAPLAAALLLYRLIYVVLPLLLACLLLLFLEARRLWVTRQAIRVASGFAAPILAILVFLSGVVLLFSGATPAIDTRLEHLGFLIPHRLIDASHLVASLIGVLCLLLAQGLRRRLSAAWALTLVLLLVGALLSLLKGFDWEEASLLSLTAALLAMFRRSFYRPSRLMEVPFSPLYVGASICVVGASVWLLLFANQDVHYSNQLWWQFALDADAPRALRAALGSCLLLLALALGWLLRAAPPAIREPNAEELQRAARIIRCSDQPDGGLALTGDKALLFHESDDAFLMYARRGRSMIALYDPIGPAMQRAELIWQFRDLCDLHHARPVFYQVRAENLPFYMDIGLTALKLGEEARVDLLRFDLENKGKEMKDLRYTWNRGQRDGLALEFHEPGQAPLDELKAISDAWLGGKQVREKGFSLGRFTPAYLNFFRIAIVRHQGKPVAFANLLETDSRELASLDLMRVHPDAPKLTMEFLMLGLILHYKAQGHARFSLGMVPLAGLQPRRGAPLTQRLGALVFRRGEQFYNFQGLRRFKDKFQPDWEPRYLAVPAGLDPLVALADTAALIAGGLTGLVKRRNSSSVDAL

Source

organism	Pseudomonas aeruginosa PAO1
ncbi_taxonomy_id	208964
host_organism	Escherichia coli

Entity 2 non-polymer

description	(1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE
formula_weight	751.0 kDa
src_method	syn
entity_count	2

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.200 Å
resolution_low	143.380 Å
r_factor	0.3705
r_work	0.3705
r_free	—

resolution	3.20 Å
resolution_method	—
num_particles	131805

fit	/app/data/saxs_computation/w5/9w51/9w51.fit
pddf	/app/data/pr_computation/w5/9w51/9w51.pr.out
profile	/app/data/saxs_computation/w5/9w51/9w51.dat

MprF from Pseudomonas aeruginosa mutant, H566C in nanodisc, C2 symmetry

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)