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9wt1

CryoEM structure of cap module in the contracted AlgoCIS

Method: ELECTRON MICROSCOPY D_max: 265.8 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9wt1
deposition_date	2025-09-15
title	CryoEM structure of cap module in the contracted AlgoCIS
keywords	Contractile injection system, CryoEM, marine bacterium, STRUCTURAL PROTEIN; STRUCTURAL PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	97.51 Å
rg_electron	95.34 Å
i0	18798400000.00
molecular_weight	1141800.0 kDa
excluded_volume	1415000 Å³
envelope_volume	2829100 Å³
shell_volume	251460 Å³
envelope_diameter	332.3 Å
shell_rg	84.02 Å
envelope_rg	97.41 Å
shape_rg	95.61 Å
total_rg	94.30 Å
total_atoms	80766
n_residues	11088
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	265.8 Å
rg_real	93.46 Å
rg_real_error	0.85 Å
i0_real	1.7990e+10
i0_real_error	2.9240e+08
rg_reciprocal	95.65 Å
i0_reciprocal	18680000000.0000
total_estimate	0.9135
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	1
r_peak_primary	99.7 Å
skewness	0.287
kurtosis	-0.512
angular_range	— – 0.0800 Å⁻¹
current_alpha	0.8706
highest_alpha	1603000000.0000
n_real_points	17
gnom_version	4.1.3
quality_criteria	AN1: 0.003; Oscil: 0.997; Stabil: 0.972; Sysdev: 1.000; Positv: 1.000; Valcen: 0.972; Smooth: 0.001

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	Pvc16 N-terminal domain-containing protein
formula_weight	22868.9 kDa
src_method	nat
entity_count	6

Polymer

type	polypeptide(L)
strand_id	A,B,C,D,E,F
atom_count	80766

MIFEVLKILTDEVNQNFKGLEMEDSEVVLNNVALIDSQQDVATELQNKVILSMINLREEVTMKNFPNNVLEGTKVTYKNPKNNINLFLIFCANRTGYKKSLSDLSRILEFFQHKSVFTQSNTSFDRDLEEMENVKNFRFTMELFTPTFEELNYIWGTLGGRQYPSVFYKLNLIVIDRDATTSEEGVITNIHRNYET

Entity 2 polymer

description	Putative phage tail sheath protein FI
formula_weight	76319.0 kDa
src_method	nat
entity_count	12

Polymer

type	polypeptide(L)
strand_id	1A,2A,1B,2B,1C,2C,1D,2D,1E,2E,1F,2F

MATYKTPGVYIEEITKFPPSVAQVETAIPAFIGYTQFARTKPSVDSDDLILKPKRISSLLDFTTYYGGAQNEQGITVKLTDTLIEGAENRTINVPEPTFKSPYLMFYSLQMYFANGGGPCYIVSTGVYDDWSDSETPPTINFSDLESGLAVIRKEDEPTLLLFPDATNLPTDDEFYSLYNSALMQCNDLQDRFTILDTYSDQTYNDGVEDLDPIPALRNGINLTKDYLKYGAAYYPFVQTILNYQYSADEIVIQHLSYNPNAIATALDNLNAVNGPTFIDAILDDLRDLSLPDISGEISDAVGFMYDDVDGFDIDGTFTTNSVKVANFASLVESVLSTLNELIDAKEEINKDVNSAIASSEEDNAIKTAISDALDVFNEDFEGADKIESVAKNLSDLLIKIKQADTNTKVENVLSINALNFSAEFEKLLTYDVNTGLTASVTLDLFANIGTRLDDIIAAVSAAEPIDVNNGKLNGRLLSDIEPLDNATYNTILLEINSHKVTLPPSSSMAGAYARVDNDRGVWKSPANIGLNYVSKPSVTVSHEEQESMNVHGTGKSVNAIRSFVGKGTLVWGARTLAGNDNEWRYISVRRFFNMAEESIKKATEQFVFEPNDGNTWVRVRAMIENFLILQWRAGALAGAKPEHAFYVKVGLGQTMTAQDILEGNMNVEIGLAVVRPAEFIILKFSHKMQES

Entity 3 polymer

description	Phage tail protein
formula_weight	16375.5 kDa
src_method	nat
entity_count	12

Polymer

type	polypeptide(L)
strand_id	1a,2a,1b,2b,1c,2c,1d,2d,1e,2e,1f,2f

MSYPLSKFHFSVEWGGTKIGFTEVSGLDLETEIIEYRHGASPEYSKIKMPGMQKFSNITLKRGTFKSDNEYFQWYNTINLNKVERRDLTISLLNEEHEPVVTWKVKNAWPLKVQSTDLKGDGNEVAIESMELAHEGLVIQNE

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6. Citations (1)

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resolution	3.20 Å
resolution_method	—
num_particles	12926

fit	/app/data/saxs_computation/wt/9wt1/9wt1.fit
pddf	/app/data/pr_computation/wt/9wt1/9wt1.pr.out
profile	/app/data/saxs_computation/wt/9wt1/9wt1.dat

CryoEM structure of cap module in the contracted AlgoCIS

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)