SAXSdb — 9wyq

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Chitin elicitor receptor kinase 1

Oryza sativa Japonica Group

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count	Chain A； UniProt 303–604	Not recorded	No other associated polymer	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；289 K；0.08 M Magnesium acetate tetrahydrate, 0.01 M MES monohydrate pH 6.0, 2.2% Polyethylene glycol 400, 8% w/v Polyethylene glycol 3,350	Resolution 1.70 Å R-free 0.236

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 9WYQ	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7VS7 Crystal structure of the ectodomain of OsCERK1 in complex with chitin hexamer Deposited 2021-10-26	Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric	Chain A 27–237（211 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；0.25M Magnesium chloride, 0.1 M TRIS, pH8.5, and 30% (w/v) PEG4000.	Resolution 2.02 Å R-free 0.235
9V70 Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa Deposited 2025-05-27	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 276–604（329 aa）	Not recorded	ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；5% v/v Polyethylene glycol 200, 0.05 M BIS-TRIS propane pH 9.0, 9% w/v Polyethylene glycol 8,000	Resolution 2.82 Å R-free 0.249
9V70 Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa Deposited 2025-05-27	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 276–604（329 aa）	Not recorded	ADP ADENOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；5% v/v Polyethylene glycol 200, 0.05 M BIS-TRIS propane pH 9.0, 9% w/v Polyethylene glycol 8,000	Resolution 2.82 Å R-free 0.249
9V70 Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa Deposited 2025-05-27	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 276–604（329 aa） Chain B 276–604（329 aa）	Not recorded	ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；5% v/v Polyethylene glycol 200, 0.05 M BIS-TRIS propane pH 9.0, 9% w/v Polyethylene glycol 8,000	Resolution 2.82 Å R-free 0.249
9V71 Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa Deposited 2025-05-27	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 303–604（302 aa）	Not recorded	ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；289 K；0.1 M Calcium acetate hydrate, 10% w/v Polyethylene glycol 3,350	Resolution 2.52 Å R-free 0.276
9V72 Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa Deposited 2025-05-27	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 303–604（302 aa） Chain B 303–604（302 aa）	Not recorded	MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；0.01 M Citric/0.025 M B-T propane ph7.1, 0.018 M Sodium malonate pH 5.0, 10.9% peg3350, 0.023 M glycyl-glycyl-glycine, 0.09 M NDSB-256	Resolution 2.75 Å R-free 0.273
9V73 Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa Deposited 2025-05-27	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 276–604（329 aa）	Not recorded	ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；0.06 M Potassium bromide, 0.02 M Ammonium acetate, 0.01 M HEPES pH 7.5, 12% w/v Polyethylene glycol monomethyl ether 2,000, 2.5% w/v Polyethylene glycol 3,350	Resolution 2.27 Å R-free 0.280
9V79 Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa Deposited 2025-05-27	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 303–604（302 aa）	Not recorded	MG MAGNESIUM ION × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；289 K；0.08 M Calcium acetate hydrate, 0.2% v/v TacsimateTM pH 4.0, 0.01 M Sodium acetate trihydrate pH 4.6, 9.6% w/v Polyethylene glycol 3,350	Resolution 2.12 Å R-free 0.275
9V7E Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa Deposited 2025-05-27	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 276–604（329 aa）	Not recorded	ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；0.06 M Potassium bromide, 0.02 M Ammonium acetate, 0.01 M Tris pH 8.5, 12% w/v Polyethylene glycol monomethyl ether 2,000, 2.5% w/v Polyethylene glycol 3,350	Resolution 1.97 Å R-free 0.272
9WYR Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa Deposited 2025-09-27	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 303–604（302 aa） Chain B 303–604（302 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；(0.009 M Citric, 0.02M B-T propane) pH 7.1, 1.1% v/v TacsimateTM pH 7.0, 0.007 M HEPES pH 7.0, 0.009 M HEPES pH 7.5, 1.8% v/v Jeffamine M-600, 0.09 M Lithium chloride, 7.5% w/v Polyethylene glycol 3,350	Resolution 2.55 Å R-free 0.270
9WYR Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa Deposited 2025-09-27	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 303–604（302 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；(0.009 M Citric, 0.02M B-T propane) pH 7.1, 1.1% v/v TacsimateTM pH 7.0, 0.007 M HEPES pH 7.0, 0.009 M HEPES pH 7.5, 1.8% v/v Jeffamine M-600, 0.09 M Lithium chloride, 7.5% w/v Polyethylene glycol 3,350	Resolution 2.55 Å R-free 0.270
9WYR Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa Deposited 2025-09-27	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 303–604（302 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；(0.009 M Citric, 0.02M B-T propane) pH 7.1, 1.1% v/v TacsimateTM pH 7.0, 0.007 M HEPES pH 7.0, 0.009 M HEPES pH 7.5, 1.8% v/v Jeffamine M-600, 0.09 M Lithium chloride, 7.5% w/v Polyethylene glycol 3,350	Resolution 2.55 Å R-free 0.270
9WYS Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa Deposited 2025-09-27	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 303–604（302 aa） Chain B 303–604（302 aa）	Not recorded	ADP ADENOSINE-5'-DIPHOSPHATE × 2 MG MAGNESIUM ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；0.04 M HEPES sodium pH 7.5, 4% v/v 2-Propanol, 0.02 M Sodium citrate tribasic dihydrate, 2% w/v Polyethylene glycol 3,350, 8% w/v Polyethylene glycol 4,000	Resolution 2.79 Å R-free 0.264
9WYS Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa Deposited 2025-09-27	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 303–604（302 aa）	Not recorded	ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；0.04 M HEPES sodium pH 7.5, 4% v/v 2-Propanol, 0.02 M Sodium citrate tribasic dihydrate, 2% w/v Polyethylene glycol 3,350, 8% w/v Polyethylene glycol 4,000	Resolution 2.79 Å R-free 0.264
9WYS Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa Deposited 2025-09-27	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 303–604（302 aa）	Not recorded	ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；0.04 M HEPES sodium pH 7.5, 4% v/v 2-Propanol, 0.02 M Sodium citrate tribasic dihydrate, 2% w/v Polyethylene glycol 3,350, 8% w/v Polyethylene glycol 4,000	Resolution 2.79 Å R-free 0.264

9 other PDB entries and 15 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	CERK1_ORYSJ
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–302； UniProt 303–604

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9wyq
Deposition date deposition_date	2025-09-27
Structure title title	Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa
Keywords keywords	dOsCERK1-KD, the kinase domain of OsCERK1 in dephosphorylated state, TRANSFERASE; TRANSFERASE
Experimental Method method	X-RAY DIFFRACTION

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	20.36 Å
Radius of gyration Rg (electron density) rg_electron	19.32 Å
Forward intensity I(0) i0	13061500.00
Molecular weight molecular_weight	28469.0 kDa
Excluded volume excluded_volume	36270 Å³
Envelope volume envelope_volume	42284 Å³
Hydration-shell volume shell_volume	18758 Å³
Envelope diameter envelope_diameter	72.0 Å
Shell Rg shell_rg	25.23 Å
Envelope Rg envelope_rg	19.55 Å
Shape Rg shape_rg	19.33 Å
Total Rg total_rg	20.22 Å
Total atoms total_atoms	2009
Residues n_residues	254
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	69.2 Å
Rg (real space) rg_real	20.36 Å
Rg uncertainty (real space) rg_real_error	0.45 Å
I(0) (real space) i0_real	1.3060e+07
I(0) uncertainty (real space) i0_real_error	1.6840e+05
Rg (reciprocal space) rg_reciprocal	20.36 Å
I(0) (reciprocal space) i0_reciprocal	13060000.0000
Solution quality estimate total_estimate	0.7927
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	3
Primary peak position r_peak_primary	24.7 Å
Skewness Skewness skewness	0.389
Kurtosis Kurtosis kurtosis	-0.174
Angular range angular_range	— – 0.3900 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	3432000.0000
Real-space data points n_real_points	71
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.772; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.988; Smooth: 0.000

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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8. Citations (1)

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Unit-cell length a length_a	58.690 Å
Unit-cell length b length_b	58.690 Å
Unit-cell length c length_c	283.190 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	120.00 °
Molecules per unit cell Z z_pdb	12

Space group space_group	P 61 2 2
Space-group number int_tables_number	178
Crystal system crystal_system	—

Resolution resolution	1.700 Å
Low-resolution limit resolution_low	29.345 Å
R factor r_factor	0.2179
R_work r_work	0.2167
R_free r_free	0.2363

Description description	Chitin elicitor receptor kinase 1
Molecular weight formula_weight	33478.2 kDa
EC 编号 ec_number	2.7.11.1
Source method src_method	man
Copy count entity_count	1

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	2212

Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa

1. Protein Identity and Related Structures Protein Identity & Related Structures

Chitin elicitor receptor kinase 1

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

6. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

Entity 2 Entity 2 water

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)

来源生物 organism	Oryza sativa Japonica Group
通用名 common_name	Japanese rice
NCBI Class编号 ncbi_taxonomy_id	39947
宿主生物 host_organism	;Escherichia coli 'BL21-Gold(DE3)pLysS AG' ;

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	203

fit	/app/data/saxs_computation/wy/9wyq/9wyq.fit
pddf	/app/data/pr_computation/wy/9wyq/9wyq.pr.out
profile	/app/data/saxs_computation/wy/9wyq/9wyq.dat