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9yck

Cryo-EM structure of MERS-CoV nsp10-nsp14 (E191A) in complex with T20P15 RNA, monomeric form

Method: ELECTRON MICROSCOPY D_max: 120.0 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9yck
deposition_date	2025-09-19
title	Cryo-EM structure of MERS-CoV nsp10-nsp14 (E191A) in complex with T20P15 RNA, monomeric form
keywords	MERS-CoV, exoribonuclease, proofreading, coronavirus, RNA, VIRAL PROTEIN, Viral Protein-RNA complex; Viral Protein/RNA
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	34.14 Å
rg_electron	34.23 Å
i0	126516000.00
molecular_weight	81484.0 kDa
excluded_volume	98103 Å³
envelope_volume	134580 Å³
shell_volume	35178 Å³
envelope_diameter	120.3 Å
shell_rg	37.73 Å
envelope_rg	34.79 Å
shape_rg	34.17 Å
total_rg	34.65 Å
total_atoms	5661
n_residues	668
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	120.0 Å
rg_real	34.46 Å
rg_real_error	1.49 Å
i0_real	1.2650e+08
i0_real_error	2.1540e+06
rg_reciprocal	34.26 Å
i0_reciprocal	126500000.0000
total_estimate	0.8295
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	33.9 Å
skewness	0.583
kurtosis	-0.255
angular_range	— – 0.2300 Å⁻¹
current_alpha	0.0000
highest_alpha	11950000.0000
n_real_points	47
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.701; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.805; Smooth: 0.870

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	Replicase polyprotein 1ab
formula_weight	14902.9 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	5661

AGSNTEFASNSSVLSLVNFTVDPQKAYLDFVNAGGAPLTNCVKMLTPKTGTGIAISVKPESTADQETYGGASVCLYCRAHIEHPDVSGVCKYKGKFVQIPAQCVRDPVGFCLSNTPCNVCQYWIGYGCNCDSLRQAALPQ

Source

organism	Middle East respiratory syndrome-related coronavirus
ncbi_taxonomy_id	1335626
host_organism	Escherichia coli

Entity 2 polymer

description	Guanine-N7 methyltransferase nsp14
formula_weight	59190.2 kDa
ec_number	2.1.1.56,3.1.13.-
mutation	E191A
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

SQIVTGLFKDCSRETSGLSPAYAPTYVSVDDKYKTSDELCVNLNLPANVPYSRVISRMGFKLDATVPGYPKLFITREEAVRQVRSWIGFDVEGAHASRNACGTNVPLQLGFSTGVNFVVQPVGVVDTEWGNMLTGIAARPPPGEQFKHLVPLMHKGAAWPIVRRRIVQMLSDTLDKLSDYCTFVCWAHGFALTSASYFCKIGKEQKCCMCNRRAAAYSSPLQSYACWTHSCGYDYVYNPFFVDVQQWGYVGNLATNHDRYCSVHQGAHVASNDAIMTRCLAIHSCFIERVDWDIEYPYISHEKKLNSCCRIVERNVVRAALLAGSFDKVYDIGNPKGIPIVDDPVVDWHYFDAQPLTRKVQQLFYTEDMASRFADGLCLFWNCNVPKYPNNAIVCRFDTRVHSEFNLPGCDGGSLYVNKHAFHTPAYDVSAFRDLKPLPFFYYSTTPCEVHGNGSMIEDIDYVPLKSAVCITACNLGGAVCRKHATEYREYMEAYNLVSASGFRLWCYKTFDIYNLWSTFTKVQ

Source

organism	Middle East respiratory syndrome-related coronavirus
ncbi_taxonomy_id	1335626
host_organism	Escherichia coli

Entity 3 polymer

description	RNA (39-MER)
formula_weight	12478.4 kDa
src_method	syn
entity_count	1

Polymer

type	polyribonucleotide
strand_id	C

GGGAAUGAUUAGGCUAAUUAUUCGUAAUUAGCCUAAUCC

Entity 4 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	5

Entity 5 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	1

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.80 Å
resolution_method	—
num_particles	141301

fit	/app/data/saxs_computation/yc/9yck/9yck.fit
pddf	/app/data/pr_computation/yc/9yck/9yck.pr.out
profile	/app/data/saxs_computation/yc/9yck/9yck.dat

Cryo-EM structure of MERS-CoV nsp10-nsp14 (E191A) in complex with T20P15 RNA, monomeric form

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Entity 4 non-polymer

Entity 5 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)