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9ycl

Cryo-EM structure of MERS-CoV nsp10-nsp14 (E191A) in complex with T20P15 RNA, dimeric form

Method: ELECTRON MICROSCOPY D_max: 156.2 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9ycl
deposition_date	2025-09-19
title	Cryo-EM structure of MERS-CoV nsp10-nsp14 (E191A) in complex with T20P15 RNA, dimeric form
keywords	MERS-CoV, exoribonuclease, proofreading, coronavirus, RNA, VIRAL PROTEIN, Viral Protein-RNA complex; Viral Protein/RNA
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	43.42 Å
rg_electron	44.02 Å
i0	480827000.00
molecular_weight	162350.0 kDa
excluded_volume	195620 Å³
envelope_volume	285270 Å³
shell_volume	57551 Å³
envelope_diameter	164.6 Å
shell_rg	44.43 Å
envelope_rg	44.78 Å
shape_rg	44.04 Å
total_rg	43.97 Å
total_atoms	11282
n_residues	1334
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	156.2 Å
rg_real	43.71 Å
rg_real_error	1.91 Å
i0_real	4.8080e+08
i0_real_error	9.8650e+06
rg_reciprocal	43.42 Å
i0_reciprocal	480700000.0000
total_estimate	0.6165
solution_quality	REASONABLE a REASONABLE solution
n_peaks	3
r_peak_primary	52.1 Å
skewness	0.591
kurtosis	0.347
angular_range	— – 0.1800 Å⁻¹
current_alpha	0.0000
highest_alpha	29360000.0000
n_real_points	37
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.753; Stabil: 1.000; Sysdev: 0.032; Positv: 1.000; Valcen: 0.919; Smooth: 0.736

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	Replicase polyprotein 1ab
formula_weight	14902.9 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,D
atom_count	11282

AGSNTEFASNSSVLSLVNFTVDPQKAYLDFVNAGGAPLTNCVKMLTPKTGTGIAISVKPESTADQETYGGASVCLYCRAHIEHPDVSGVCKYKGKFVQIPAQCVRDPVGFCLSNTPCNVCQYWIGYGCNCDSLRQAALPQ

Source

organism	Middle East respiratory syndrome-related coronavirus
ncbi_taxonomy_id	1335626
host_organism	Escherichia coli

Entity 2 polymer

description	Guanine-N7 methyltransferase nsp14
formula_weight	59190.2 kDa
ec_number	2.1.1.56,3.1.13.-
mutation	E191A
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	B,E

SQIVTGLFKDCSRETSGLSPAYAPTYVSVDDKYKTSDELCVNLNLPANVPYSRVISRMGFKLDATVPGYPKLFITREEAVRQVRSWIGFDVEGAHASRNACGTNVPLQLGFSTGVNFVVQPVGVVDTEWGNMLTGIAARPPPGEQFKHLVPLMHKGAAWPIVRRRIVQMLSDTLDKLSDYCTFVCWAHGFALTSASYFCKIGKEQKCCMCNRRAAAYSSPLQSYACWTHSCGYDYVYNPFFVDVQQWGYVGNLATNHDRYCSVHQGAHVASNDAIMTRCLAIHSCFIERVDWDIEYPYISHEKKLNSCCRIVERNVVRAALLAGSFDKVYDIGNPKGIPIVDDPVVDWHYFDAQPLTRKVQQLFYTEDMASRFADGLCLFWNCNVPKYPNNAIVCRFDTRVHSEFNLPGCDGGSLYVNKHAFHTPAYDVSAFRDLKPLPFFYYSTTPCEVHGNGSMIEDIDYVPLKSAVCITACNLGGAVCRKHATEYREYMEAYNLVSASGFRLWCYKTFDIYNLWSTFTKVQ

Source

organism	Middle East respiratory syndrome-related coronavirus
ncbi_taxonomy_id	1335626
host_organism	Escherichia coli

Entity 3 polymer

description	T20P15 RNA
formula_weight	12478.4 kDa
src_method	syn
entity_count	2

Polymer

type	polyribonucleotide
strand_id	C,F

GGGAAUGAUUAGGCUAAUUAUUCGUAAUUAGCCUAAUCC

Entity 4 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	10

Entity 5 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	2

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.50 Å
resolution_method	—
num_particles	84344

fit	/app/data/saxs_computation/yc/9ycl/9ycl.fit
pddf	/app/data/pr_computation/yc/9ycl/9ycl.pr.out
profile	/app/data/saxs_computation/yc/9ycl/9ycl.dat

Cryo-EM structure of MERS-CoV nsp10-nsp14 (E191A) in complex with T20P15 RNA, dimeric form

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Entity 4 non-polymer

Entity 5 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)