当前蛋白身份：O00214
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 2YRO Solution structure of the C-terminal Gal-bind lectin protein from Human Galectin-8 提交 2007-04-02 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
175–316（142 aa）
片段：Gal-bind_lectin domain
|
未记录 | 未记录非水小分子 | SOLUTION NMR |
NMR测量条件
pH 7；296 K；离子强度（mmCIF原始值）120mM；压力 ambient
NMR样品组成
1.28mM Gal-binding lectin U-15N, 13C; 20mM d-Tris-HCl(pH 7.0); 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 90% H2O, 10% D2O | 90% H2O/10% D2O
|
分辨率未提供 |
| 3AP4 Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with lactose 提交 2010-10-11 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–154（154 aa）
片段：N-terminal carbohydrate recognition domain
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；0.275mM protein, 10mM Hepes-NaOH, 2.75mM lactose, 50mM sodium chloride, 0.5mM DTT, 50mM ammonium fluoride, 12% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K
|
分辨率 2.33 Å R-free 0.251 |
| 3AP4 Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with lactose 提交 2010-10-11 | Assembly 2 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–154（154 aa）
片段：N-terminal carbohydrate recognition domain
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；0.275mM protein, 10mM Hepes-NaOH, 2.75mM lactose, 50mM sodium chloride, 0.5mM DTT, 50mM ammonium fluoride, 12% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K
|
分辨率 2.33 Å R-free 0.251 |
| 3AP4 Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with lactose 提交 2010-10-11 | Assembly 3 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 C
1–154（154 aa）
片段：N-terminal carbohydrate recognition domain
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；0.275mM protein, 10mM Hepes-NaOH, 2.75mM lactose, 50mM sodium chloride, 0.5mM DTT, 50mM ammonium fluoride, 12% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K
|
分辨率 2.33 Å R-free 0.251 |
| 3AP4 Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with lactose 提交 2010-10-11 | Assembly 4 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 D
1–154（154 aa）
片段：N-terminal carbohydrate recognition domain
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；0.275mM protein, 10mM Hepes-NaOH, 2.75mM lactose, 50mM sodium chloride, 0.5mM DTT, 50mM ammonium fluoride, 12% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K
|
分辨率 2.33 Å R-free 0.251 |
| 3AP5 Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain 提交 2010-10-11 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–154（154 aa）
片段：N-terminal carbohydrate recognition domain
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；0.275mM protein, 10mM Hepes-NaOH, 50mM sodium chloride, 0.5mM DTT, 125mM ammonium fluoride, 8% PEG 3350 , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K
|
分辨率 1.92 Å R-free 0.237 |
| 3AP6 Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with lactose 3'-sulfate 提交 2010-10-12 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–154（154 aa）
片段：N-terminal carbohydrate recognition domain
|
未记录 | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；0.275mM protein, 10mM Hepes-NaOH, 2.75mM lactose 3'-sulfate, 50mM sodium chloride, 0.5mM DTT, 50mM ammonium fluoride, 9% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K
|
分辨率 1.58 Å R-free 0.242 |
| 3AP6 Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with lactose 3'-sulfate 提交 2010-10-12 | Assembly 2 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–154（154 aa）
片段：N-terminal carbohydrate recognition domain
|
未记录 | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；0.275mM protein, 10mM Hepes-NaOH, 2.75mM lactose 3'-sulfate, 50mM sodium chloride, 0.5mM DTT, 50mM ammonium fluoride, 9% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K
|
分辨率 1.58 Å R-free 0.242 |
| 3AP6 Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with lactose 3'-sulfate 提交 2010-10-12 | Assembly 3 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 C
1–154（154 aa）
片段：N-terminal carbohydrate recognition domain
|
未记录 | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；0.275mM protein, 10mM Hepes-NaOH, 2.75mM lactose 3'-sulfate, 50mM sodium chloride, 0.5mM DTT, 50mM ammonium fluoride, 9% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K
|
分辨率 1.58 Å R-free 0.242 |
| 3AP6 Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with lactose 3'-sulfate 提交 2010-10-12 | Assembly 4 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 D
1–154（154 aa）
片段：N-terminal carbohydrate recognition domain
|
未记录 | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；0.275mM protein, 10mM Hepes-NaOH, 2.75mM lactose 3'-sulfate, 50mM sodium chloride, 0.5mM DTT, 50mM ammonium fluoride, 9% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K
|
分辨率 1.58 Å R-free 0.242 |
| 3AP7 Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with lactose sialic acid 提交 2010-10-12 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–154（154 aa）
片段：N-terminal carbohydrate recognition domain
|
未记录 | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；0.275mM protein, 10mM Hepes-NaOH, 2.75mM lactose sialic acid, 50mM sodium chloride, 0.5mM DTT, 80mM ammonium
fluoride, 7.5% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K
|
分辨率 1.53 Å R-free 0.188 |
| 3AP9 Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with Lacto-N-fucopentaose III 提交 2010-10-12 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–154（154 aa）
片段：N-terminal carbohydrate recognition domain
|
未记录 | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 CL CHLORIDE ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；0.275mM protein, 10mM Hepes-NaOH, 2.75mM Lacto-N-fucopentaose III, 50mM sodium chloride, 0.5mM DTT, 50mM ammonium fluoride, 12% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K
|
分辨率 1.33 Å R-free 0.178 |
| 3APB Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with iodide 提交 2010-10-14 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–154（154 aa）
片段：N-terminal carbohydrate recognition domain
|
未记录 | IOD IODIDE ION × 13 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；0.55mM protein, 10mM Hepes-NaOH, 50mM sodium chloride, 0.5mM DTT, 125mM ammonium fluoride, 8% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K
|
分辨率 1.95 Å R-free 0.242 |
| 3APB Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with iodide 提交 2010-10-14 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–154（154 aa）
片段：N-terminal carbohydrate recognition domain
|
未记录 | IOD IODIDE ION × 16 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；0.55mM protein, 10mM Hepes-NaOH, 50mM sodium chloride, 0.5mM DTT, 125mM ammonium fluoride, 8% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K
|
分辨率 1.95 Å R-free 0.242 |
| 3OJB Crystal structure of C-terminal domain of human galectin-8 提交 2010-08-21 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
186–315（130 aa）
片段：C-terminal domain
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；281 K；27.5% PEG 8000, 0.2M ammonium phosphate, 6.5% Ethylene Glycol, 0.1M Na Cacodylate, VAPOR DIFFUSION, HANGING DROP, temperature 281K
|
分辨率 3.01 Å R-free 0.292 |
| 3OJB Crystal structure of C-terminal domain of human galectin-8 提交 2010-08-21 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
186–315（130 aa）
片段：C-terminal domain
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；281 K；27.5% PEG 8000, 0.2M ammonium phosphate, 6.5% Ethylene Glycol, 0.1M Na Cacodylate, VAPOR DIFFUSION, HANGING DROP, temperature 281K
|
分辨率 3.01 Å R-free 0.292 |
| 3OJB Crystal structure of C-terminal domain of human galectin-8 提交 2010-08-21 | Assembly 3 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 C
186–315（130 aa）
片段：C-terminal domain
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；281 K；27.5% PEG 8000, 0.2M ammonium phosphate, 6.5% Ethylene Glycol, 0.1M Na Cacodylate, VAPOR DIFFUSION, HANGING DROP, temperature 281K
|
分辨率 3.01 Å R-free 0.292 |
| 3OJB Crystal structure of C-terminal domain of human galectin-8 提交 2010-08-21 | Assembly 4 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 D
186–315（130 aa）
片段：C-terminal domain
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；281 K；27.5% PEG 8000, 0.2M ammonium phosphate, 6.5% Ethylene Glycol, 0.1M Na Cacodylate, VAPOR DIFFUSION, HANGING DROP, temperature 281K
|
分辨率 3.01 Å R-free 0.292 |
| 3VKL Protease-resistant mutant form of human Galectin-8 in complex with two lactose molecules 提交 2011-11-18 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–155（155 aa）
片段：N-terminal carbohydrate recognition domain, C-terminal carbohydrate recognition domain
链 A
184–317（134 aa）
片段：N-terminal carbohydrate recognition domain, C-terminal carbohydrate recognition domain
|
未记录 | EDO 1,2-ETHANEDIOL × 12 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；2-3%(v/v) 1,4-dioxane, 9-10%(w/v) PEG20000, 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.55 Å R-free 0.264 |
| 3VKL Protease-resistant mutant form of human Galectin-8 in complex with two lactose molecules 提交 2011-11-18 | Assembly 2 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–155（155 aa）
片段：N-terminal carbohydrate recognition domain, C-terminal carbohydrate recognition domain
链 B
184–317（134 aa）
片段：N-terminal carbohydrate recognition domain, C-terminal carbohydrate recognition domain
|
未记录 | EDO 1,2-ETHANEDIOL × 8 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；2-3%(v/v) 1,4-dioxane, 9-10%(w/v) PEG20000, 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.55 Å R-free 0.264 |
| 3VKM Protease-resistant mutant form of Human Galectin-8 in complex with sialyllactose and lactose 提交 2011-11-18 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–155（155 aa）
片段：N-terminal carbohydrate recognition domain, C-terminal carbohydrate recognition domain
链 A
184–317（134 aa）
片段：N-terminal carbohydrate recognition domain, C-terminal carbohydrate recognition domain
|
未记录 | EDO 1,2-ETHANEDIOL × 11 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；2-3%(v/v) 1,4-dioxane, 9-10%(w/v) PEG20000, 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.98 Å R-free 0.261 |
| 3VKM Protease-resistant mutant form of Human Galectin-8 in complex with sialyllactose and lactose 提交 2011-11-18 | Assembly 2 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–155（155 aa）
片段：N-terminal carbohydrate recognition domain, C-terminal carbohydrate recognition domain
链 B
184–317（134 aa）
片段：N-terminal carbohydrate recognition domain, C-terminal carbohydrate recognition domain
|
未记录 | EDO 1,2-ETHANEDIOL × 13 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；2-3%(v/v) 1,4-dioxane, 9-10%(w/v) PEG20000, 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.98 Å R-free 0.261 |
| 3VKN Galectin-8 N-terminal domain in free form 提交 2011-11-18 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–153（153 aa）
片段：N-TERMINAL CARBOHYDRATE RECOGNITION DOMAIN
|
未记录 | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 9.5；293 K；30%(v/v) PEG 400, 0.1M CHES, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
分辨率 1.98 Å R-free 0.251 |
| 3VKN Galectin-8 N-terminal domain in free form 提交 2011-11-18 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–153（153 aa）
片段：N-TERMINAL CARBOHYDRATE RECOGNITION DOMAIN
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 9.5；293 K；30%(v/v) PEG 400, 0.1M CHES, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
分辨率 1.98 Å R-free 0.251 |
| 3VKO Galectin-8 N-terminal domain in complex with sialyllactosamine 提交 2011-11-18 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–153（153 aa）
片段：N-TERMINAL CARBOHYDRATE RECOGNITION DOMAIN
|
未记录 | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 9.5；293 K；30%(v/v) PEG400, 0.1M CHES, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
分辨率 2.08 Å R-free 0.249 |
| 3VKO Galectin-8 N-terminal domain in complex with sialyllactosamine 提交 2011-11-18 | Assembly 2 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–153（153 aa）
片段：N-TERMINAL CARBOHYDRATE RECOGNITION DOMAIN
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 9.5；293 K；30%(v/v) PEG400, 0.1M CHES, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
分辨率 2.08 Å R-free 0.249 |
| 4BMB Crystal structure of the N terminal domain of human Galectin 8 提交 2013-05-07 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
4–153（150 aa）
片段：N TERMINAL DOMAIN, RESIDUES 4-153
|
未记录 | ZN ZINC ION × 1 NA SODIUM ION × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
20% W/V PEG 4000, 100 MM HEPES SODIUM SALT (PH 7.2) AND 10 MM ZINC CHLORIDE
|
分辨率 1.35 Å R-free 0.177 |
| 4BME Crystal structure of the N terminal domain of human Galectin 8, F19Y mutant 提交 2013-05-07 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
4–155（152 aa）
片段：N TERMINAL DOMAIN, RESIDUES 4-155
|
突变：YES | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
30% W/V PEG 4000, 100 MM MES SODIUM SALT (PH 6.5)
|
分辨率 2.00 Å R-free 0.241 |
| 4BME Crystal structure of the N terminal domain of human Galectin 8, F19Y mutant 提交 2013-05-07 | Assembly 2 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
4–155（152 aa）
片段：N TERMINAL DOMAIN, RESIDUES 4-155
|
突变：YES | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
30% W/V PEG 4000, 100 MM MES SODIUM SALT (PH 6.5)
|
分辨率 2.00 Å R-free 0.241 |
| 4FQZ Crystal structure of a protease-resistant mutant form of human galectin-8 提交 2012-06-26 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–155（155 aa）
片段：N-terminal carbohydrate recognition domain, C-terminal carbohydrate recognition domain
链 A
184–317（134 aa）
片段：N-terminal carbohydrate recognition domain, C-terminal carbohydrate recognition domain
|
未记录 | EDO 1,2-ETHANEDIOL × 10 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；10% PEG 20000, 0.1M Bicine, 2% Dioxane, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.80 Å R-free 0.253 |
| 4GXL The crystal structure of Galectin-8 C-CRD in complex with NDP52 提交 2012-09-04 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
186–317（132 aa）
片段：UNP residues 186-317
|
未记录 | GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.5；283 K；2% PEG 400, 0.1M HEPES sodium pH 7.5, 2.0M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 283K
|
分辨率 2.02 Å R-free 0.216 |
| 4HAN Crystal structure of Galectin 8 with NDP52 peptide 提交 2012-09-27 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
1–155（155 aa）
片段：UNP residues 1-155. 184-317
链 A
184–317（134 aa）
片段：UNP residues 1-155. 184-317
链 B
1–155（155 aa）
片段：UNP residues 1-155. 184-317
链 B
184–317（134 aa）
片段：UNP residues 1-155. 184-317
|
未记录 | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 2 PEG DI(HYDROXYETHYL)ETHER × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；0.1M Tris-Hcl pH8.5, 30~34% (v/w) PEG400, 200mM LiSO4, and 10mM Nicotinamide adenine dinucleotide, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
分辨率 2.55 Å R-free 0.214 |
| 5GZC Crystal structure of Galectin-8 N-CRD with part of linker 提交 2016-09-28 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
7–158（152 aa）
片段：carbohydrate recognition domain (UNP RESIDUES 7-158)
|
未记录 | GOL GLYCEROL × 1 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；298 K；PEG 2000MME
|
分辨率 1.08 Å R-free 0.163 |
| 5GZD Galectin-8 N-terminal domain carbohydrate recognition domain 提交 2016-09-28 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
7–154（148 aa）
片段：carbohydrate recognition domain (UNP RESIDUES 7-154)
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；298 K；PEG 2000MME
|
分辨率 1.19 Å R-free 0.212 |
| 5GZE Galectin-8 N-terminal domain carbohydrate recognition domain 提交 2016-09-28 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
7–154（148 aa）
片段：carbohydrate recognition domain (UNP RESIDUES 7-154)
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；298 K；PEG 2000MME
|
分辨率 1.32 Å R-free 0.278 |
| 5GZF Galectin-8 N-terminal domain carbohydrate recognition domain 提交 2016-09-28 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–186（186 aa）
片段：carbohydrate recognition domain (UNP RESIDUES 1-186)
|
未记录 | NA SODIUM ION × 1 NI NICKEL (II) ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；0.1M Tris pH 8.5, 0.01M NiCl2, 20% (W/V) PEG 2000 MME
|
分辨率 2.00 Å R-free 0.218 |
| 5GZG Galectin-8 N-terminal domain carbohydrate recognition domain 提交 2016-09-28 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–186（186 aa）
片段：carbohydrate recognition domain (UNP RESIDUES 1-186)
|
未记录 | NA SODIUM ION × 1 NI NICKEL (II) ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；298 K；0.1M Tris pH 8.5, 0.01M NiCl2,20% (W/V) PEG2000 MME
|
分辨率 2.00 Å R-free 0.258 |
| 5T7I Crystal structure of galectin-8N in complex with LNnT 提交 2016-09-05 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–155（155 aa）
片段：N-terminal Domaine (UNP residues 1-155)
|
非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；293 K；10 mM sodium phosphate, 137 mM sodium chloride, 2.7 mM potassium chloride, 1.8 mM potassium phosphate
|
分辨率 2.00 Å R-free 0.217 |
| 5T7S Crystal structure of galectin-8N in complex with Lactose 提交 2016-09-05 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–155（155 aa）
片段：N-terminal Domaine (UNP residues 1-155)
|
突变：M56V 非标准单体：是（mmCIF未提供具体位点） | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；293 K；10 mM sodium phosphate, 137 mM sodium chloride, 2.7 mM potassium chloride, 1.8 mM potassium phosphate
|
分辨率 1.90 Å R-free 0.180 |
| 5T7T Galectin-8 N terminal domain in complex with LNT 提交 2016-09-05 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–155（155 aa）
片段：N-terminal Domaine (UNP residues 1-155)
|
突变：M56V 非标准单体：是（mmCIF未提供具体位点） | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；293 K；10 mM sodium phosphate, 137 mM sodium chloride, 2.7 mM potassium chloride, 1.8 mM potassium phosphate
|
分辨率 1.96 Å R-free 0.215 |
| 5T7U Crystal structure of galectin-8N in complex with Glycerol 提交 2016-09-05 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–155（155 aa）
片段：N-terminal Domaine (UNP residues 1-155)
|
非标准单体：是（mmCIF未提供具体位点） | GOL GLYCEROL × 2 NA SODIUM ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；293 K；10 mM sodium phosphate, 137 mM sodium chloride, 2.7 mM potassium chloride, 1.8 mM potassium phosphate
|
分辨率 1.58 Å R-free 0.147 |
| 5VWG Galectin-8 N terminal domain in complex with Methyl 3-O-[1-carboxyethyl]-beta-D-galactopyranoside 提交 2017-05-21 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–155（155 aa）
片段：N-terminal domain (UNP residues 1-155)
|
突变：M56V 非标准单体：是（mmCIF未提供具体位点） | 9QG methyl 3-O-[(1R)-1-carboxyethyl]-beta-D-galactopyranoside × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；277 K；10 mM sodium phosphate, 137 mM sodium chloride, 2.7 mM potassium chloride, 1.8 mM potassium phosphate
|
分辨率 2.10 Å R-free 0.251 |
| 6W4Z Galectin-8N terminal domain in complex with Methyl 3-O-[3-O-benzyloxy]-malonyl-beta-D-galactopyranoside 提交 2020-03-12 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
2–154（153 aa）
|
突变：M54V | SZS methyl 3-O-[3-(benzyloxy)-3-oxopropanoyl]-beta-D-galactopyranoside × 1 CL CHLORIDE ION × 1 EDO 1,2-ETHANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1M sodium citrate dihydrate pH 5.6, 20% PEG 4000, 10% IPA
|
分辨率 1.59 Å R-free 0.226 |
| 6W4Z Galectin-8N terminal domain in complex with Methyl 3-O-[3-O-benzyloxy]-malonyl-beta-D-galactopyranoside 提交 2020-03-12 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
2–154（153 aa）
|
突变：M54V | SZS methyl 3-O-[3-(benzyloxy)-3-oxopropanoyl]-beta-D-galactopyranoside × 1 EDO 1,2-ETHANEDIOL × 1 PG4 TETRAETHYLENE GLYCOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1M sodium citrate dihydrate pH 5.6, 20% PEG 4000, 10% IPA
|
分辨率 1.59 Å R-free 0.226 |
| 6Z6Y Galectin-8 N-terminal domain in complex with a sulfatide mimicking a sphingolipid 提交 2020-05-29 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
5–160（156 aa）
|
未记录 | L4L [(2~{R},3~{R},4~{S},5~{S},6~{R})-2-[(2~{S})-2-acetamido-3-oxidanylidene-pent-4-enoxy]-6-(hydroxymethyl)-3,5-bis(oxidanyl)oxan-4-yl] hydrogen sulfate × 1 ACT ACETATE ION × 6 PGE TRIETHYLENE GLYCOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.6；298 K；0.1 M Na-HEPES, pH 7.6, 10% PEG 4000 and 20% Isopropanol
|
分辨率 1.34 Å R-free 0.194 |
| 7AEN Galectin-8 N-terminal carbohydrate recognition domain in complex with methyl 3-O-((7-carboxy)quinolin-2-yl)-methoxy)-beta-D-galactopyranoside 提交 2020-09-17 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
7–156（150 aa）
|
未记录 | R8B methyl 3-O-((7-carboxy) quinolin-2-yl)-methyl)-beta-D-galactopyranoside × 1 GOL GLYCEROL × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
LIQUID DIFFUSION；pH 8；293.5 K；buffer (10mM Tris/HCl pH 8.0, 150 mM NaCl, 10 mm lactose and 1 mM TCEP) mixed with reservoir 25% (w/v) PEG 2000 monomethylethyer (MME), a cryo solution (10 mM Tris/HCl pH 8.0, 50 mM NaCl, 10 mM lactose, 25% (w/v) PEG 2000 MME, 20% ethylene glycol (EG) and 1 mM TCEP) and flash-frozen in liquid nitrogen.
A co-crystal with lactose, grown from a seeded drop with 24% (w/v) PEG 2000 MME in the reservoir, was used to soak in compound 1 by transferring crystals in three steps to different soaking drops. First to a 2 ul drop with glycerol ( 20% (v/v) glycerol, 25% (w/v PEG 2000 MME, 10 mM Tris/HCl pH 8.0, 50 mM NaCl and 1 mM TCEP) and secondly to a 2 ul drop with 10 mM compound 1 (10 mM Tris/HCl pH 8.0, 50 mM NaCl, 5 mM compound 1, 25% (w/v) PEG 2000 MME and 1 mM TCEP) and thirdly to a second drop with 5 mM compound 1 (same composition as before).
|
分辨率 1.60 Å R-free 0.212 |
| 7AEN Galectin-8 N-terminal carbohydrate recognition domain in complex with methyl 3-O-((7-carboxy)quinolin-2-yl)-methoxy)-beta-D-galactopyranoside 提交 2020-09-17 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
7–156（150 aa）
|
未记录 | R8B methyl 3-O-((7-carboxy) quinolin-2-yl)-methyl)-beta-D-galactopyranoside × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
LIQUID DIFFUSION；pH 8；293.5 K；buffer (10mM Tris/HCl pH 8.0, 150 mM NaCl, 10 mm lactose and 1 mM TCEP) mixed with reservoir 25% (w/v) PEG 2000 monomethylethyer (MME), a cryo solution (10 mM Tris/HCl pH 8.0, 50 mM NaCl, 10 mM lactose, 25% (w/v) PEG 2000 MME, 20% ethylene glycol (EG) and 1 mM TCEP) and flash-frozen in liquid nitrogen.
A co-crystal with lactose, grown from a seeded drop with 24% (w/v) PEG 2000 MME in the reservoir, was used to soak in compound 1 by transferring crystals in three steps to different soaking drops. First to a 2 ul drop with glycerol ( 20% (v/v) glycerol, 25% (w/v PEG 2000 MME, 10 mM Tris/HCl pH 8.0, 50 mM NaCl and 1 mM TCEP) and secondly to a 2 ul drop with 10 mM compound 1 (10 mM Tris/HCl pH 8.0, 50 mM NaCl, 5 mM compound 1, 25% (w/v) PEG 2000 MME and 1 mM TCEP) and thirdly to a second drop with 5 mM compound 1 (same composition as before).
|
分辨率 1.60 Å R-free 0.212 |
| 7ALS Alpha and beta Lactose isomer structure of Galectin 8, N-terminal domain 提交 2020-10-07 | Assembly 1 其他组合 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
4–158（155 aa）
链 B
4–158（155 aa）
|
未记录 | CL CHLORIDE ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；293.5 K；24% (w/v) PEG 2000 MME
|
分辨率 1.35 Å R-free 0.199 |
| 7P11 Galectin-8 N-terminal carbohydrate recognition domain in complex with quinoline D-galactal ligand 提交 2021-07-01 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
7–156（150 aa）
|
未记录 | 5KM 2-[[(2~{R},3~{R},4~{R})-2-(hydroxymethyl)-3-oxidanyl-3,4-dihydro-2~{H}-pyran-4-yl]oxymethyl]quinoline-7-carboxylic acid × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
LIQUID DIFFUSION；pH 8；293.5 K；Buffer (10mM Tris/HCl pH 8.0, 150 mM NaCl, 10 mm lactose and 1 mM TCEP) mixed with reservoir 25% (w/v) PEG 2000 monomethylethyer (MME), a cryo solution (10 mM Tris/HCl pH 8.0, 50 mM NaCl, 10 mM lactose, 25% (w/v) PEG 2000 MME, 20% ethylene glycol (EG) and 1 mM TCEP) and flash-frozen in liquid nitrogen.
A co-crystal with lactose, grown from a seeded drop with 24% (w/v) PEG 2000 MME in the reservoir, was used to soak in compound 1 by transferring crystals in three steps to different soaking drops. First to a 2 ul drop with glycerol ( 20% (v/v) glycerol, 25% (w/v PEG 2000 MME, 10 mM Tris/HCl pH 8.0, 50 mM NaCl and 1 mM TCEP) and secondly to a 2 ul drop with 10 mM compound 1 (10 mM Tris/HCl pH 8.0, 50 mM NaCl, 5 mM compound 1, 25% (w/v) PEG 2000 MME and 1 mM TCEP) and thirdly to a second drop with 5 mM compound 1 (same composition as before).
|
分辨率 2.10 Å R-free 0.263 |
| 7P11 Galectin-8 N-terminal carbohydrate recognition domain in complex with quinoline D-galactal ligand 提交 2021-07-01 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
6–156（151 aa）
|
未记录 | 5KM 2-[[(2~{R},3~{R},4~{R})-2-(hydroxymethyl)-3-oxidanyl-3,4-dihydro-2~{H}-pyran-4-yl]oxymethyl]quinoline-7-carboxylic acid × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
LIQUID DIFFUSION；pH 8；293.5 K；Buffer (10mM Tris/HCl pH 8.0, 150 mM NaCl, 10 mm lactose and 1 mM TCEP) mixed with reservoir 25% (w/v) PEG 2000 monomethylethyer (MME), a cryo solution (10 mM Tris/HCl pH 8.0, 50 mM NaCl, 10 mM lactose, 25% (w/v) PEG 2000 MME, 20% ethylene glycol (EG) and 1 mM TCEP) and flash-frozen in liquid nitrogen.
A co-crystal with lactose, grown from a seeded drop with 24% (w/v) PEG 2000 MME in the reservoir, was used to soak in compound 1 by transferring crystals in three steps to different soaking drops. First to a 2 ul drop with glycerol ( 20% (v/v) glycerol, 25% (w/v PEG 2000 MME, 10 mM Tris/HCl pH 8.0, 50 mM NaCl and 1 mM TCEP) and secondly to a 2 ul drop with 10 mM compound 1 (10 mM Tris/HCl pH 8.0, 50 mM NaCl, 5 mM compound 1, 25% (w/v) PEG 2000 MME and 1 mM TCEP) and thirdly to a second drop with 5 mM compound 1 (same composition as before).
|
分辨率 2.10 Å R-free 0.263 |
| 7P1M Galectin-8 N-terminal carbohydrate recognition domain in complex with benzimidazole D-galactal ligand 提交 2021-07-02 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
6–156（151 aa）
链 B
6–156（151 aa）
|
未记录 | 4IU 2-[[(2R,3R,4R)-2-(hydroxymethyl)-3-oxidanyl-3,4-dihydro-2H-pyran-4-yl]oxymethyl]-3-methyl-benzimidazole-5-carboxylic acid × 2 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
LIQUID DIFFUSION；pH 8；293.5 K；Buffer (10mM Tris/HCl pH 8.0, 150 mM NaCl, 10 mm lactose and 1 mM TCEP) mixed with reservoir 25% (w/v) PEG 2000 monomethylethyer (MME), a cryo solution (10 mM Tris/HCl pH 8.0, 50 mM NaCl, 10 mM lactose, 25% (w/v) PEG 2000 MME, 20% ethylene glycol (EG) and 1 mM TCEP) and flash-frozen in liquid nitrogen.
A co-crystal with lactose, grown from a seeded drop with 24% (w/v) PEG 2000 MME in the reservoir, was used to soak in compound 1 by transferring crystals in three steps to different soaking drops. First to a 2 ul drop with glycerol ( 20% (v/v) glycerol, 25% (w/v PEG 2000 MME, 10 mM Tris/HCl pH 8.0, 50 mM NaCl and 1 mM TCEP) and secondly to a 2 ul drop with 10 mM compound 1 (10 mM Tris/HCl pH 8.0, 50 mM NaCl, 5 mM compound 1, 25% (w/v) PEG 2000 MME and 1 mM TCEP) and thirdly to a second drop with 5 mM compound 1 (same composition as before).
|
分辨率 1.52 Å R-free 0.207 |
| 8HL9 Crystal structure of Galectin-8 C-CRD with part of linker 提交 2022-11-29 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
171–317（147 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；293 K；0.1M Hepes pH 7.0, 0.2M MgCl2, 30% (W/V) PEG 3350
|
分辨率 1.60 Å R-free 0.251 |
| 9FXZ Galectin-8 N-terminal carbohydrate recognition domain in complex with 4-(bromophenyl)phthalazinone D-galactal ligand 提交 2024-07-02 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–317（317 aa）
|
未记录 | V19 4-(4-bromophenyl)-2-[[(2~{R},3~{R},4~{R})-2-(hydroxymethyl)-3-oxidanyl-3,4-dihydro-2~{H}-pyran-4-yl]oxymethyl]phthalazin-1-one × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8；293.5 K；A co-crystal with lactose, grown from 12 mg/ml galectin-8N in 10 mM lactose, 10 mM Tris/HCl pH 8.0, 1 mM TCEP and 150 mM sodium chloride mixed with 25% (w/v) PEG 2000 MME) in a hanging drop, was used to soak the compound by transferring crystals in three steps to soaking drops. These were 2 uL drops with a soaking solution of 20% ethylene glycol, 25% PEG 2000 MME, 10 mM Tris pH 8, 1 mM TCEP, 50 mM NaCl, and 2 mM compound 10. The incubation times in the first two drops were about 10 min and in the third drop about 24 h. Then the crystals were transferred to a cryo solution with the same constituents and flash-frozen in liquid nitrogen.
|
分辨率 1.30 Å R-free 0.200 |
| 9FXZ Galectin-8 N-terminal carbohydrate recognition domain in complex with 4-(bromophenyl)phthalazinone D-galactal ligand 提交 2024-07-02 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–317（317 aa）
|
未记录 | V19 4-(4-bromophenyl)-2-[[(2~{R},3~{R},4~{R})-2-(hydroxymethyl)-3-oxidanyl-3,4-dihydro-2~{H}-pyran-4-yl]oxymethyl]phthalazin-1-one × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8；293.5 K；A co-crystal with lactose, grown from 12 mg/ml galectin-8N in 10 mM lactose, 10 mM Tris/HCl pH 8.0, 1 mM TCEP and 150 mM sodium chloride mixed with 25% (w/v) PEG 2000 MME) in a hanging drop, was used to soak the compound by transferring crystals in three steps to soaking drops. These were 2 uL drops with a soaking solution of 20% ethylene glycol, 25% PEG 2000 MME, 10 mM Tris pH 8, 1 mM TCEP, 50 mM NaCl, and 2 mM compound 10. The incubation times in the first two drops were about 10 min and in the third drop about 24 h. Then the crystals were transferred to a cryo solution with the same constituents and flash-frozen in liquid nitrogen.
|
分辨率 1.30 Å R-free 0.200 |
| 9FYJ N-terminal domain of human galectin-8 in complex with an alpha-galactoside ligand 提交 2024-07-03 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
4–156（153 aa）
链 B
4–156（153 aa）
|
未记录 | A1IHE 2-[[(2~{R},3~{R},4~{S},5~{S},6~{R})-2-(3,4-dichlorophenyl)sulfanyl-6-(hydroxymethyl)-5-oxidanyl-3-prop-2-ynoxy-oxan-4-yl]oxymethyl]-3-methyl-benzimidazole-5-carboxylic acid × 2 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Drops consisted of 2 ul galectin-8N at 13.1 mg/ml in PBS buffer, 1 mM ligand, 5% DMSO mixed with 2 ul of reservoir solution consisting of 35% (w/v) Morpheus precipitant mix 4, 0.16 M Morpheus alcohol mix, 61 mM MES, 39 mM imidazole, pH 7.5. Morpheus precipitant mix 4 is made up of 25% (w/v) each of methane pentane diol (MPD), polyethylene glycol (PEG) 1000 and PEG 3350. Morpheus alcohol mix contains 0.2 M each of 1,6-hexanediol, 1-butanol, 1,2-propanediol, 2-propanol, 1,4-butanediol and 1,3-propanediol (1.2 M total).
|
分辨率 1.08 Å R-free 0.150 |
| 9KH4 Crystal structure of Galectin-8 N-CRD with N-acetyllactosamine 提交 2024-11-09 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
4–153（150 aa）
|
未记录 | NA SODIUM ION × 1 RNQ ~{N}-[(2~{R},3~{R},4~{R},5~{S},6~{R})-6-(hydroxymethyl)-5-[(2~{S},3~{R},4~{S},5~{R},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-2,4-bis(oxidanyl)oxan-3-yl]ethanamide × 1 NI NICKEL (II) ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；298 K；PEG
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分辨率 1.75 Å R-free 0.212 |