Current Protein Identity：P00171
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1CYO BOVINE CYTOCHROME B(5) Deposited 1994-08-03 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
5–97（93 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.50 Å |
| 1EHB CRYSTAL STRUCTURE OF RECOMBINANT TRYPSIN-SOLUBILIZED FRAGMENT OF CYTOCHROME B5 Deposited 2000-02-20 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
8–89（82 aa）
Fragment：TRYPSIN-SOLUBILIZED FRAGMENT
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.90 Å R-free 0.248 |
| 1ES1 CRYSTAL STRUCTURE OF VAL61HIS MUTANT OF TRYPSIN-SOLUBILIZED FRAGMENT OF CYTOCHROME B5 Deposited 2000-04-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
8–89（82 aa）
Fragment：TRYPSIN-SOLUBILIZED FRAGMENT
|
Mutation：VAL61HIS | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；phosphate buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.10 Å R-free 0.244 |
| 1F03 SOLUTION STRUCTURE OF OXIDIZED BOVINE MICROSOMAL CYTOCHROME B5 MUTANT (E44A, E48A, E56A, D60A) AND ITS INTERACTION WITH CYTOCHROME C Deposited 2000-05-14 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
7–88（82 aa）
|
Mutation：E44A, E48A, E56A, D60A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | SOLUTION NMR |
NMR measurement conditions
pH 7；303 K；Ionic strength (raw mmCIF value) 10 mM；Pressure 1
NMR sample composition
6 mM cytochrome b5 mutant 1H | 10 mM phosphate buffer, 90% H2O, 10% D2O
NMR sample composition
6 mM cytochrome b5 mutant 1H | 10 mM phosphate buffer, D2O
|
Resolution not provided |
| 1F04 SOLUTION STRUCTURE OF OXIDIZED BOVINE MICROSOMAL CYTOCHROME B5 MUTANT (E44A, E48A, E56A, D60A) AND ITS INTERACTION WITH CYTOCHROME C Deposited 2000-05-14 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
7–88（82 aa）
|
Mutation：E44A, E48A, E56A, D60A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | SOLUTION NMR |
NMR measurement conditions
pH 7；303 K；Ionic strength (raw mmCIF value) 10 mM；Pressure 1
NMR sample composition
6 mM cytochrome b5 mutant 1H | 10 mM phosphate buffer , 90% H2O, 10% D2O
NMR sample composition
6 mM cytochrome b5 mutant 1H | 10 mM phosphate buffer, D2O
|
Resolution not provided |
| 1HKO NMR structure of bovine cytochrome b5 Deposited 2003-03-10 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–104（104 aa）
Fragment：HEME BINDING DOMAIN, RESIDUES 1-104
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | SOLUTION NMR |
NMR measurement conditions
pH 7；300 K
|
Resolution not provided |
| 1I5U SOLUTION STRUCTURE OF CYTOCHROME B5 TRIPLE MUTANT (E48A/E56A/D60A) Deposited 2001-02-28 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
8–89（82 aa）
Fragment：SOLUBLE DOMAIN (RESIDUES 8-89)
|
Mutation：E48A/E56A/D60A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | SOLUTION NMR |
NMR measurement conditions
pH 7；303 K；Pressure 1
NMR measurement conditions
pH 7；303 K；Pressure 1
NMR measurement conditions
pH 7；303 K；Pressure 1
NMR measurement conditions
pH 7；303 K；Pressure 1
NMR sample composition
4mM cytochrome b5 triple mutant(E48A/E56A/D60A) | 90% H2O/10% D2O
|
Resolution not provided |
| 1J0Q Solution Structure of Oxidized Bovine Microsomal Cytochrome b5 mutant V61H Deposited 2002-11-20 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
7–88（82 aa）
Fragment：Trypsin-Solubilized Fragment
|
Mutation：V61H | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | SOLUTION NMR |
NMR measurement conditions
pH 7；293 K；Pressure ambient
NMR sample composition
2.9mM enzyme, pH 7.0, 25mM phosphate buffer | 90% H2O/10% D2O
|
Resolution not provided |
| 1LQX Crystal structure of V45E mutant of cytochrome b5 Deposited 2002-05-14 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
7–88（82 aa）
Fragment：trypsin-solubilized fragment of cytochrome b5
|
Mutation：V45E | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；293 K；PHOSPHATE BUFFER, pH 7.5, VAPOR DIFFUSION, temperature 293.0K
|
Resolution 1.80 Å R-free 0.222 |
| 1LR6 Crystal structure of V45Y mutant of cytochrome b5 Deposited 2002-05-15 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
7–88（82 aa）
Fragment：trypsin-solubilized fragment of cytochrome b5
|
Mutation：V45Y | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；PHOSPHATE BUFFER, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 1.90 Å R-free 0.236 |
| 1M20 Crystal Structure of F35Y Mutant of Trypsin-solubilized Fragment of Cytochrome b5 Deposited 2002-06-20 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
7–88（82 aa）
Fragment：trypsin-solubilized fragment
|
Mutation：F35Y | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；phosphate buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.227 |
| 1M2I Crystal structure of E44A/E56A mutant of cytochrome b5 Deposited 2002-06-24 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
7–88（82 aa）
Fragment：trypsin-solubilized fragment of cytochrome b5
|
Mutation：E44A, E56A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；PHOSPHATE BUFFER, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 1.80 Å R-free 0.232 |
| 1M2M Crystal structure of E44A/E48A/E56A/D60A mutant of cytochrome b5 Deposited 2002-06-24 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
7–88（82 aa）
Fragment：trypsin-solubilized fragment of cytochrome b5
|
Mutation：E44A, E48A, E56A, D60A | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；PHOSPHATE BUFFER, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.238 |
| 1M59 Crystal Structure of P40V Mutant of Trypsin-solubilized Fragment of Cytochrome b5 Deposited 2002-07-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
7–88（82 aa）
Fragment：trypsin-solubilized fragment
|
Mutation：P40V | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；phosphate buffer, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.90 Å R-free 0.227 |
| 1NX7 Solution Structure of Oxidized Bovine Microsomal Cytochrome B5 Deposited 2003-02-10 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
7–88（82 aa）
Fragment：RESIDUES 3-84
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | SOLUTION NMR |
NMR measurement conditions
pH 7；300 K；Ionic strength (raw mmCIF value) 25mM Phosphate buffer；Pressure 1
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 25mM Phosphate buffer；Pressure 1
NMR sample composition
3.0mM | 90% H2O/10% D2O
NMR sample composition
3.0mM | 100% D2O
|
Resolution not provided |
| 1SH4 Solution structure of oxidized bovine microsomal cytochrome B5 Mutant V45H Deposited 2004-02-25 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
7–88（82 aa）
Fragment：residues 3-84
|
Mutation：V45H | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | SOLUTION NMR |
NMR measurement conditions
pH 7；293 K；Ionic strength (raw mmCIF value) 25mM PHOSPHATE BUFFER；Pressure 1
NMR sample composition
3.0mM | 90% H2O/10% D2O
NMR sample composition
3.0mM | 100% D2O
|
Resolution not provided |
| 1U9M Crystal structure of F58W mutant of cytochrome b5 Deposited 2004-08-10 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
7–88（82 aa）
Fragment：trypsin-solubilized fragment
|
Mutation：F58W | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；phosphate buffer, dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.266 |
| 1U9M Crystal structure of F58W mutant of cytochrome b5 Deposited 2004-08-10 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
7–88（82 aa）
Fragment：trypsin-solubilized fragment
|
Mutation：F58W | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；phosphate buffer, dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.266 |
| 1U9M Crystal structure of F58W mutant of cytochrome b5 Deposited 2004-08-10 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
7–88（82 aa）
Fragment：trypsin-solubilized fragment
|
Mutation：F58W | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；phosphate buffer, dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.266 |
| 1U9M Crystal structure of F58W mutant of cytochrome b5 Deposited 2004-08-10 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
7–88（82 aa）
Fragment：trypsin-solubilized fragment
|
Mutation：F58W | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；phosphate buffer, dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.266 |
| 1U9M Crystal structure of F58W mutant of cytochrome b5 Deposited 2004-08-10 | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain E
7–88（82 aa）
Fragment：trypsin-solubilized fragment
|
Mutation：F58W | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；phosphate buffer, dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.266 |
| 1U9M Crystal structure of F58W mutant of cytochrome b5 Deposited 2004-08-10 | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain F
7–88（82 aa）
Fragment：trypsin-solubilized fragment
|
Mutation：F58W | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；phosphate buffer, dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.266 |
| 1U9U Crystal structure of F58Y mutant of cytochrome b5 Deposited 2004-08-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
7–88（82 aa）
Fragment：Trypsin-solubilized fragment
|
Mutation：F58Y | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；phosphate buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.86 Å R-free 0.215 |
| 3OZZ Structure of a cytochrome b5 core-swap mutant Deposited 2010-09-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
8–89（82 aa）
Fragment：N-TERMINAL HEME-BINDING DOMAIN (unp residues 8-89)
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；277 K；30% PEG8000, 0.2M MgAc, 0.1M Pipes, pH 7.0, VAPOR DIFFUSION, temperature 277K
|
Resolution 1.70 Å R-free 0.229 |
| 4HIN 2.4A Resolution Structure of Bovine Cytochrome b5 (S71L) Deposited 2012-10-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
8–89（82 aa）
|
Mutation：S71L | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CU COPPER (II) ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；293 K；20% (w/v) PEG 8000, 100 mM Tris, 200 mM MgCl2, 10 mM CuCl2, pH 8.5, vapor diffusion, temperature 293K
|
Resolution 2.40 Å R-free 0.245 |
| 4HIN 2.4A Resolution Structure of Bovine Cytochrome b5 (S71L) Deposited 2012-10-11 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
8–89（82 aa）
|
Mutation：S71L | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CU COPPER (II) ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；293 K；20% (w/v) PEG 8000, 100 mM Tris, 200 mM MgCl2, 10 mM CuCl2, pH 8.5, vapor diffusion, temperature 293K
|
Resolution 2.40 Å R-free 0.245 |
| 4HIN 2.4A Resolution Structure of Bovine Cytochrome b5 (S71L) Deposited 2012-10-11 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
8–89（82 aa）
|
Mutation：S71L | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CU COPPER (II) ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；293 K；20% (w/v) PEG 8000, 100 mM Tris, 200 mM MgCl2, 10 mM CuCl2, pH 8.5, vapor diffusion, temperature 293K
|
Resolution 2.40 Å R-free 0.245 |
| 4HIN 2.4A Resolution Structure of Bovine Cytochrome b5 (S71L) Deposited 2012-10-11 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
8–89（82 aa）
|
Mutation：S71L | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CU COPPER (II) ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；293 K；20% (w/v) PEG 8000, 100 mM Tris, 200 mM MgCl2, 10 mM CuCl2, pH 8.5, vapor diffusion, temperature 293K
|
Resolution 2.40 Å R-free 0.245 |