Current Protein Identity：P01033
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1D2B THE MMP-INHIBITORY, N-TERMINAL DOMAIN OF HUMAN TISSUE INHIBITOR OF METALLOPROTEINASES-1 (N-TIMP-1), SOLUTION NMR, 29 STRUCTURES Deposited 1999-09-22 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
24–149（126 aa）
Fragment：NTR domain, residues 24-149
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6；293 K；Ionic strength (raw mmCIF value) 0.171；Pressure AMBIENT
NMR sample composition
0.5 MM N-TIMP-1 U-15N
NMR sample composition
0.9 MM N-TIMP-1 U-15N,13C
NMR sample composition
0.7 MM N-TIMP-1 U-15N,13C
NMR sample composition
0.4 MM N-TIMP-1 16% 13C
NMR sample composition
0.4 MM N-TIMP-1 U-15N,13C
|
Resolution not provided |
| 1OO9 Orientation in Solution of MMP-3 Catalytic Domain and N-TIMP-1 from Residual Dipolar Couplings Deposited 2003-03-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
24–149（126 aa）
Fragment：N-TERMINAL DOMAIN
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.6；310 K；Ionic strength (raw mmCIF value) 20mM Tris-d11; 100 mM NaCl; 15 mM CaCl2; 3uM ZnCl2; 1mM Sodium Azide；Pressure ambient
NMR measurement conditions
pH 6.7；307 K；Ionic strength (raw mmCIF value) 20mM Tris-d11; 125mM NaCl; 15 mM CaCl2; 50uM ZnCl2;1mM Sodium Azide；Pressure ambient
NMR sample composition
0.8mM MMP-3 U-15N, 13C; 0.8mM N-TIMP-1;
20mM Tris-d11; 100 mM NaCl; 15 mM CaCl2; 3uM ZnCl2;
1mM Sodium Azide; 93% H2O, 7% D2O | 93% H2O/7% D2O
NMR sample composition
0.5mM MMP-3 U-2H, 15N; 0.5mM N-TIMP-1;
20mM Tris-d11; 100 mM NaCl; 15 mM CaCl2; 3uM ZnCl2;
1mM Sodium Azide; 93% H2O, 7% D2O | 93% H2O/7% D2O
NMR sample composition
0.66mM N-TIMP-1 U-15N, 13C; 0.66mM MMP-3(E202Q);
20mM Tris-d11; 125mM NaCl; 15 mM CaCl2; 50uM ZnCl2;
1mM Sodium Azide; 93% H2O; 7% D2O | 93% H2O/7% D2O
NMR sample composition
0.3mM 98% 2H/15N N-TIMP-1; 0.3mM MMP-3 (E202Q);
20mM Tris-d11; 125mM NaCl; 15 mM CaCl2; 50uM ZnCl2;
1mM Sodium Azide; 93% H2O; 7% D2O | 93% H2O/7% D2O
NMR sample composition
0.3mM 98% 2H/15N N-TIMP-1; 0.3mM (15N-IV, 15N, 13C-L)MMP-3(E202Q);
20mM Tris-d11; 125mM NaCl; 15 mM CaCl2; 50uM ZnCl2;
1mM Sodium Azide; 93% H2O; 7% D2O | 93% H2O/7% D2O
NMR sample composition
0.3mM 98% 2H/15N N-TIMP-1; 0.3mM (15N-IV, 15N, 13C-L)MMP-3(E202Q);
20mM Tris-d11; 125mM NaCl; 15 mM CaCl2; 50uM ZnCl2;
1mM Sodium Azide; 93% H2O; 7% D2O
5% PEG(C12E6)/1-hexanol | 93% H2O; 7% D2O
5% PEG(C12E6)/1-hexanol
|
Resolution not provided |
| 1UEA MMP-3/TIMP-1 COMPLEX Deposited 1997-06-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
24–207（184 aa）
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 3 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.80 Å |
| 1UEA MMP-3/TIMP-1 COMPLEX Deposited 1997-06-06 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
24–207（184 aa）
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 3 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.80 Å |
| 2J0T Crystal Structure of the Catalytic Domain of MMP-1 in Complex with the Inhibitory Domain of TIMP-1 Deposited 2006-08-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
24–149（126 aa）
Fragment：N-TERMINAL INHIBITORY DOMAIN, RESIDUES 24-149
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；10% PEG 8000, 8% ETHYLENE GLYCOL, 0.1M HEPES (PH 7.5).
|
Resolution 2.54 Å R-free 0.275 |
| 2J0T Crystal Structure of the Catalytic Domain of MMP-1 in Complex with the Inhibitory Domain of TIMP-1 Deposited 2006-08-04 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
24–149（126 aa）
Fragment：N-TERMINAL INHIBITORY DOMAIN, RESIDUES 24-149
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；10% PEG 8000, 8% ETHYLENE GLYCOL, 0.1M HEPES (PH 7.5).
|
Resolution 2.54 Å R-free 0.275 |
| 2J0T Crystal Structure of the Catalytic Domain of MMP-1 in Complex with the Inhibitory Domain of TIMP-1 Deposited 2006-08-04 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
24–149（126 aa）
Fragment：N-TERMINAL INHIBITORY DOMAIN, RESIDUES 24-149
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；10% PEG 8000, 8% ETHYLENE GLYCOL, 0.1M HEPES (PH 7.5).
|
Resolution 2.54 Å R-free 0.275 |
| 3MA2 Complex membrane type-1 matrix metalloproteinase (MT1-MMP) with tissue inhibitor of metalloproteinase-1 (TIMP-1) Deposited 2010-03-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
24–148（125 aa）
Fragment：Residues 24-148
|
Mutation：V27A,P29V,T121L | CA CALCIUM ION × 2 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；292 K；100 mM Bis-Tris pH 5.5, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.05 Å R-free 0.247 |
| 3MA2 Complex membrane type-1 matrix metalloproteinase (MT1-MMP) with tissue inhibitor of metalloproteinase-1 (TIMP-1) Deposited 2010-03-23 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
24–148（125 aa）
Fragment：Residues 24-148
|
Mutation：V27A,P29V,T121L | CA CALCIUM ION × 2 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；292 K；100 mM Bis-Tris pH 5.5, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.05 Å R-free 0.247 |
| 3V96 Complex of matrix metalloproteinase-10 catalytic domain (MMP-10cd) with tissue inhibitor of metalloproteinases-1 (TIMP-1) Deposited 2011-12-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
24–207（184 aa）
Fragment：unp residues 24-207
|
Mutation：N30A | PO4 PHOSPHATE ION × 2 ZN ZINC ION × 2 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；298 K；0.1 M sodium dihydrogen phosphate solution, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.266 |
| 6MAV Complex of tissue inhibitor of metalloproteinase-1 (TIMP-1) mutant L34G with matrix metalloproteinase-3 catalytic domain (MMP-3cd) Deposited 2018-08-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
24–207（184 aa）
|
Mutation：L34G | CA CALCIUM ION × 3 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；0.1 M Ammonium Acetate 0.1 M Bis-Tris: HCl, pH 5.5, 17 % (w/v) PEG 10,000
|
Resolution 2.37 Å R-free 0.298 |
| 6N9D Complex of tissue inhibitor of metalloproteinases-1 (TIMP-1) mutant (L34G/L133P/L151C/G154A) with matrix metalloproteinase-3 catalytic domain (MMP-3cd) Deposited 2018-12-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
24–207（184 aa）
|
Not recorded | CA CALCIUM ION × 3 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2 M Sodium Acetate, 0.1 M Sodium Cacodylate: HCl, pH 6.5 18 % (w/v) PEG 8000
|
Resolution 2.67 Å R-free 0.258 |
| 7S7L Complex of tissue inhibitor of metalloproteinases-1 (TIMP-1) mutant (L34G/M66S/E67Y/L133N/S155L) with matrix metalloproteinase-3 catalytic domain (MMP-3cd) Deposited 2021-09-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
24–207（184 aa）
|
Mutation：L34G, M66S, E67Y, L133N, S155L | CA CALCIUM ION × 3 ZN ZINC ION × 2 CL CHLORIDE ION × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 SO4 SULFATE ION × 1 GOL GLYCEROL × 1 ACT ACETATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；310 K；0.2M ammonium sulfate, 0.1M Bis-Tris pH 6.5, 25% w/v PEG 3350
Anatrace-Microlytic Top96 #65
|
Resolution 2.34 Å R-free 0.239 |
| 7S7M Complex of tissue inhibitor of metalloproteinases-1 (TIMP-1) mutant (L34G/M66D/T98G/P131S/Q153N) with matrix metalloproteinase-3 catalytic domain (MMP-3cd) Deposited 2021-09-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
24–207（184 aa）
|
Mutation：L34G, M66D, T98G, P131S, Q153N | CA CALCIUM ION × 3 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；310 K；0.1 M ammonium acetate, 0.1 M Bis-Tris-HCl, pH 5.5, 17% w/v PEG10000
|
Resolution 3.00 Å R-free 0.248 |
| 9SOP Tissue inhibitor of metalloproteinase-1 (TIMP-1) Deposited 2025-09-15 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
24–203（180 aa）
|
Not recorded | GOL GLYCEROL × 2 EDO 1,2-ETHANEDIOL × 3 FLC CITRATE ANION × 1 P3G 3,6,9,12,15-PENTAOXAHEPTADECANE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.4；293.15 K；0.2 M Lithium Citrate tribasic tetrahydrate,
20% PEG 3350,
15% Glycerol
|
Resolution 1.95 Å R-free 0.239 |
| 9SOP Tissue inhibitor of metalloproteinase-1 (TIMP-1) Deposited 2025-09-15 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
24–203（180 aa）
|
Not recorded | GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 5 PGE TRIETHYLENE GLYCOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.4；293.15 K；0.2 M Lithium Citrate tribasic tetrahydrate,
20% PEG 3350,
15% Glycerol
|
Resolution 1.95 Å R-free 0.239 |
| 9SOQ Tissue inhibitor of Metalloproteinase 1 (TIMP-1) Deposited 2025-09-15 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
24–207（184 aa）
|
Not recorded | FLC CITRATE ANION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.6；293.15 K；0.2 Ammonium Sulphate,
0.1 M tri-sodium citrate,
25% PEG4000
|
Resolution 2.30 Å R-free 0.262 |
| 9SOQ Tissue inhibitor of Metalloproteinase 1 (TIMP-1) Deposited 2025-09-15 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
24–207（184 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.6；293.15 K；0.2 Ammonium Sulphate,
0.1 M tri-sodium citrate,
25% PEG4000
|
Resolution 2.30 Å R-free 0.262 |
| 9SOS Tissue Inhibitor of Matrix Metalloproteinase-1 (TIMP-1) Deposited 2025-09-15 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
24–207（184 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.4；293.15 K；0.2 M Lithium Citrate tribasic tetrahydrate,
20% PEG3350
|
Resolution 2.00 Å R-free 0.255 |