Current Protein Identity：P01555
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1S5B Cholera holotoxin with an A-subunit Y30S mutation Form 3 Deposited 2004-01-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
19–258（240 aa）
|
Mutation：Y30S | NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；PEG 2000mme, MES, kemptide, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.13 Å R-free 0.224 |
| 1S5C Cholera holotoxin with an A-subunit Y30S mutation, Crystal form 1 Deposited 2004-01-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
19–258（240 aa）
|
Mutation：Y30S | NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；PEG 2000mme, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.50 Å R-free 0.262 |
| 1S5D Cholera holotoxin with an A-subunit Y30S mutation, Crystal form 2 Deposited 2004-01-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
19–258（240 aa）
|
Mutation：Y30S | NA SODIUM ION × 1 GOL GLYCEROL × 5 GAL beta-D-galactopyranose × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；PEG 3350, sodium citrate, galactose, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.75 Å R-free 0.191 |
| 1S5E Cholera holotoxin, Crystal form 1 Deposited 2004-01-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
19–258（240 aa）
|
Not recorded | NA SODIUM ION × 1 GAL beta-D-galactopyranose × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；PEG 3350, magnesium acetate, galactose, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.205 |
| 1S5E Cholera holotoxin, Crystal form 1 Deposited 2004-01-20 | Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
19–258（240 aa）
|
Not recorded | NA SODIUM ION × 1 GAL beta-D-galactopyranose × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；PEG 3350, magnesium acetate, galactose, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.205 |
| 1S5F Cholera holotoxin, Crystal form 2 Deposited 2004-01-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
19–258（240 aa）
|
Not recorded | NA SODIUM ION × 1 GAL beta-D-galactopyranose × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；PEG 3350, lithium citrate, galactose, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.60 Å R-free 0.260 |
| 1S5F Cholera holotoxin, Crystal form 2 Deposited 2004-01-20 | Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
19–258（240 aa）
|
Not recorded | NA SODIUM ION × 1 GAL beta-D-galactopyranose × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；PEG 3350, lithium citrate, galactose, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.60 Å R-free 0.260 |
| 1XTC CHOLERA TOXIN Deposited 1996-01-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain A
19–212（194 aa）
Chain C
213–258（46 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.40 Å |
| 2A5D Structural basis for the activation of cholera toxin by human ARF6-GTP Deposited 2005-06-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
19–210（192 aa）
Fragment：A1 subunit
|
Mutation：E110D, E112D, C187S | MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NA SODIUM ION × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；PEG2000mme, cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.80 Å R-free 0.199 |
| 2A5F Cholera toxin A1 subunit bound to its substrate, NAD+, and its human protein activator, ARF6 Deposited 2005-06-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
19–210（192 aa）
Fragment：Cholera toxin A1 subunit
|
Mutation：E110D, E112D, C187S | MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NA SODIUM ION × 1 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；PEG2000mme, cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.02 Å R-free 0.231 |
| 2A5G Cholera toxin A1 subunit bound to ARF6(Q67L) Deposited 2005-06-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
19–210（192 aa）
Fragment：Cholera toxin A1 subunit
|
Mutation：E110D, E112D, C187S | MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；PEG 2000mme, cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.66 Å R-free 0.265 |
| 8OXS Cholera holotoxin variant (chimera with E. coli heat-labile enterotoxin, 4 C-terminal substitutions) Deposited 2023-05-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
19–258（240 aa）
|
Mutation：D229E, I230V, T232I, H233Y | NA SODIUM ION × 1 GAL beta-D-galactopyranose × 6 GLA alpha-D-galactopyranose × 4 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；Crystallization mix:
20% w/v PEG 6K
0.1 M HEPES pH 7.0
0.2 M NH4Cl
Protein storage buffer:
0.05 M Tris/HCl pH 7.5
0.2 M NaCl
0.05 M D-(+)-galactose
|
Resolution 1.60 Å R-free 0.224 |
| 8OXS Cholera holotoxin variant (chimera with E. coli heat-labile enterotoxin, 4 C-terminal substitutions) Deposited 2023-05-02 | Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
19–258（240 aa）
|
Mutation：D229E, I230V, T232I, H233Y | NA SODIUM ION × 1 GAL beta-D-galactopyranose × 4 GLA alpha-D-galactopyranose × 3 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；Crystallization mix:
20% w/v PEG 6K
0.1 M HEPES pH 7.0
0.2 M NH4Cl
Protein storage buffer:
0.05 M Tris/HCl pH 7.5
0.2 M NaCl
0.05 M D-(+)-galactose
|
Resolution 1.60 Å R-free 0.224 |
| 8Q6I Cholera holotoxin variant (chimera with E. coli heat-labile enterotoxin, 1 C-terminal substitution) Deposited 2023-08-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
19–258（240 aa）
|
Mutation：D229E Non-standard monomer：Yes (specific site not provided by mmCIF) | NA SODIUM ION × 2 GOL GLYCEROL × 11 PEG DI(HYDROXYETHYL)ETHER × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293.15 K；Crystallization mix:
20% PEG6000
0.2 M lithium chloride
0.1 M Tris pH 8.0
Protein storage buffer:
0.05 M Tris/HCl pH 7.5
0.2 M NaCl
|
Resolution 1.60 Å R-free 0.222 |
| 8QRE Cholera holotoxin (wildtype) Deposited 2023-10-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
19–258（240 aa）
|
Not recorded | NA SODIUM ION × 2 GAL beta-D-galactopyranose × 6 GOL GLYCEROL × 7 ACT ACETATE ION × 2 PEG DI(HYDROXYETHYL)ETHER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；293.15 K；Crystallization mix:
0.125 M Magnesium acetate
24% PEG 3350
300 mM galactose (in protein storage buffer)
Protein storage buffer:
50 mM Tris pH 7.4
200 mM NaCl
1 mM EDTA
3 mM sodium azide
|
Resolution 2.30 Å R-free 0.263 |
| 8QRE Cholera holotoxin (wildtype) Deposited 2023-10-06 | Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
19–258（240 aa）
|
Not recorded | NA SODIUM ION × 2 GAL beta-D-galactopyranose × 4 GOL GLYCEROL × 7 ACT ACETATE ION × 1 GLA alpha-D-galactopyranose × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；293.15 K；Crystallization mix:
0.125 M Magnesium acetate
24% PEG 3350
300 mM galactose (in protein storage buffer)
Protein storage buffer:
50 mM Tris pH 7.4
200 mM NaCl
1 mM EDTA
3 mM sodium azide
|
Resolution 2.30 Å R-free 0.263 |