Current Protein Identity：P04695
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1AQG NMR STRUCTURE OF THE RHODOPSIN-BOUND C-TERMINAL PEPTIDE OF THE TRANSDUCIN ALPHA-SUBUNIT, 20 STRUCTURES Deposited 1997-07-29 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
339–349（11 aa）
Fragment：RHODOPSIN BINDING DOMAIN, RESIDUES 340-350
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.5；274 K；Ionic strength (raw mmCIF value) 120；Pressure AMBIENT
NMR sample composition
20 mM sodium phosphate, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 1FQJ CRYSTAL STRUCTURE OF THE HETEROTRIMERIC COMPLEX OF THE RGS DOMAIN OF RGS9, THE GAMMA SUBUNIT OF PHOSPHODIESTERASE AND THE GT/I1 CHIMERA ALPHA SUBUNIT [(RGS9)-(PDEGAMMA)-(GT/I1ALPHA)-(GDP)-(ALF4-)-(MG2+)] Deposited 2000-09-05 | Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
26–215（190 aa）
Fragment：UNP P04695 residues 26-215 and 295-350 linked via UNP P10824 residues 220-298
Chain A
295–350（56 aa）
Fragment：UNP P04695 residues 26-215 and 295-350 linked via UNP P10824 residues 220-298
|
Not recorded | MG MAGNESIUM ION × 1 ALF TETRAFLUOROALUMINATE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；15.0% PEG8000, 200mM Tris pH9.0, 0.2% beta-ME, 1mM (NH4)2WS4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.02 Å R-free 0.265 |
| 1FQJ CRYSTAL STRUCTURE OF THE HETEROTRIMERIC COMPLEX OF THE RGS DOMAIN OF RGS9, THE GAMMA SUBUNIT OF PHOSPHODIESTERASE AND THE GT/I1 CHIMERA ALPHA SUBUNIT [(RGS9)-(PDEGAMMA)-(GT/I1ALPHA)-(GDP)-(ALF4-)-(MG2+)] Deposited 2000-09-05 | Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
26–215（190 aa）
Fragment：UNP P04695 residues 26-215 and 295-350 linked via UNP P10824 residues 220-298
Chain D
295–350（56 aa）
Fragment：UNP P04695 residues 26-215 and 295-350 linked via UNP P10824 residues 220-298
|
Not recorded | MG MAGNESIUM ION × 1 ALF TETRAFLUOROALUMINATE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；15.0% PEG8000, 200mM Tris pH9.0, 0.2% beta-ME, 1mM (NH4)2WS4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.02 Å R-free 0.265 |
| 1FQK CRYSTAL STRUCTURE OF THE HETERODIMERIC COMPLEX OF THE RGS DOMAIN OF RGS9, AND THE GT/I1 CHIMERA ALPHA SUBUNIT [(RGS9)-(GT/I1ALPHA)-(GDP)-(ALF4-)-(MG2+)] Deposited 2000-09-05 | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
26–215（190 aa）
Fragment：UNP P04695 residues 26-215 and 295-350 linked via UNP P10824 residues 220-298
Chain A
295–350（56 aa）
Fragment：UNP P04695 residues 26-215 and 295-350 linked via UNP P10824 residues 220-298
|
Not recorded | MG MAGNESIUM ION × 1 ALF TETRAFLUOROALUMINATE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；277 K；10.5% PEG8000, 110mM magnesium acetate, 50mM Tris pH 8.5, 0.2% beta-ME, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.30 Å R-free 0.268 |
| 1FQK CRYSTAL STRUCTURE OF THE HETERODIMERIC COMPLEX OF THE RGS DOMAIN OF RGS9, AND THE GT/I1 CHIMERA ALPHA SUBUNIT [(RGS9)-(GT/I1ALPHA)-(GDP)-(ALF4-)-(MG2+)] Deposited 2000-09-05 | Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
26–215（190 aa）
Fragment：UNP P04695 residues 26-215 and 295-350 linked via UNP P10824 residues 220-298
Chain C
295–350（56 aa）
Fragment：UNP P04695 residues 26-215 and 295-350 linked via UNP P10824 residues 220-298
|
Not recorded | MG MAGNESIUM ION × 1 ALF TETRAFLUOROALUMINATE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；277 K；10.5% PEG8000, 110mM magnesium acetate, 50mM Tris pH 8.5, 0.2% beta-ME, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.30 Å R-free 0.268 |
| 1GOT HETEROTRIMERIC COMPLEX OF A GT-ALPHA/GI-ALPHA CHIMERA AND THE GT-BETA-GAMMA SUBUNITS Deposited 1996-08-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
1–349（349 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；10 MG/ML OF HETEROTRIMERIC COMPLEX WERE MIXED 1:1 WITH WELL SOLUTION CONTAINING 10% PEG-8000, 50 MM TRIS, PH 8.0, 10% GLYCEROL, 50 MM NACL, .1 MM MERCAPTOETHANOL. MIXTURE EQUILIBRATED VS WELL SOLUTION IN HANGING DROPS AT 4 DEGREES C., vapor diffusion - hanging drop, temperature 277K
|
Resolution 2.00 Å R-free 0.295 |
| 1LVZ METARHODOPSIN II BOUND STRUCTURE OF C-TERMINAL PEPTIDE OF ALPHA-SUBUNIT OF TRANSDUCIN Deposited 2002-05-30 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
339–349（11 aa）
Fragment：S2 Peptide, Residues 339-349
|
Mutation：K340R, C346S | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.6；283 K；Ionic strength (raw mmCIF value) 10 mM HEPES, 20 mM KCl；Pressure ambient
NMR sample composition
2.6mM S2 peptide U-15N, 0.063mM rhodopsin as part of intact disk membranes from bovine retina; buffer: 10 mM HEPES, 20mM KCl, 0.05mM DTPA | 90% H2O/10% D2O
NMR sample composition
2.6mM S2 peptide U-15N, 13C, 0.063mM rhodopsin as part of intact disk membranes from bovine retina; buffer: 10 mM HEPES, 20mM KCl, 0.05mM DTPA | 90% H2O/10% D2O
|
Resolution not provided |
| 1TAD GTPASE MECHANISM OF GPROTEINS FROM THE 1.7-ANGSTROM CRYSTAL STRUCTURE OF TRANSDUCIN ALPHA-GDP-ALF4- Deposited 1995-01-05 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
26–349（324 aa）
|
Not recorded | CA CALCIUM ION × 1 CAC CACODYLATE ION × 2 ALF TETRAFLUOROALUMINATE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.70 Å R-free 0.266 |
| 1TAD GTPASE MECHANISM OF GPROTEINS FROM THE 1.7-ANGSTROM CRYSTAL STRUCTURE OF TRANSDUCIN ALPHA-GDP-ALF4- Deposited 1995-01-05 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
26–349（324 aa）
|
Not recorded | CA CALCIUM ION × 1 CAC CACODYLATE ION × 2 ALF TETRAFLUOROALUMINATE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.70 Å R-free 0.266 |
| 1TAD GTPASE MECHANISM OF GPROTEINS FROM THE 1.7-ANGSTROM CRYSTAL STRUCTURE OF TRANSDUCIN ALPHA-GDP-ALF4- Deposited 1995-01-05 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
26–349（324 aa）
|
Not recorded | CA CALCIUM ION × 1 CAC CACODYLATE ION × 2 ALF TETRAFLUOROALUMINATE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.70 Å R-free 0.266 |
| 1TAG STRUCTURAL DETERMINANTS FOR ACTIVATION OF THE ALPHA-SUBUNIT OF A HETEROTRIMERIC G PROTEIN Deposited 1994-11-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
26–349（324 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.80 Å R-free 0.211 |
| 1TND THE 2.2 ANGSTROMS CRYSTAL STRUCTURE OF TRANSDUCIN-ALPHA COMPLEXED WITH GTP GAMMA S Deposited 1994-03-31 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
26–349（324 aa）
|
Not recorded | MG MAGNESIUM ION × 1 CAC CACODYLATE ION × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1TND THE 2.2 ANGSTROMS CRYSTAL STRUCTURE OF TRANSDUCIN-ALPHA COMPLEXED WITH GTP GAMMA S Deposited 1994-03-31 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
26–349（324 aa）
|
Not recorded | MG MAGNESIUM ION × 1 CAC CACODYLATE ION × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1TND THE 2.2 ANGSTROMS CRYSTAL STRUCTURE OF TRANSDUCIN-ALPHA COMPLEXED WITH GTP GAMMA S Deposited 1994-03-31 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
26–349（324 aa）
|
Not recorded | MG MAGNESIUM ION × 1 CAC CACODYLATE ION × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 2X72 CRYSTAL STRUCTURE OF THE CONSTITUTIVELY ACTIVE E113Q,D2C,D282C RHODOPSIN MUTANT WITH BOUND GALPHACT PEPTIDE. Deposited 2010-02-22 | Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
340–350（11 aa）
Fragment：RESIDUES 340-350
|
Mutation：YES | PEF DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE × 2 BOG octyl beta-D-glucopyranoside × 2 PLM PALMITIC ACID × 4 RET RETINAL × 2 LPP 2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE × 2 ACT ACETATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 4.5；3.0-3.4 M AMMONIUM SULFATE, 100 MM SODIUM ACETATE PH 4.5
|
Resolution 3.00 Å R-free 0.244 |
| 3DQB Crystal structure of the active G-protein-coupled receptor opsin in complex with a C-terminal peptide derived from the Galpha subunit of transducin Deposited 2008-07-09 | Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
340–350（11 aa）
Fragment：C-terminal domain, UNP residues 340-350
|
Mutation：K341L | BOG octyl beta-D-glucopyranoside × 3 PLM PALMITIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；AMMONIUM SULFATE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 3.20 Å R-free 0.248 |
| 3PQR Crystal structure of Metarhodopsin II in complex with a C-terminal peptide derived from the Galpha subunit of transducin Deposited 2010-11-26 | Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
340–350（11 aa）
Fragment：C-terminal peptide (UNP residues 340-350)
|
Mutation：K341L,C347V | RET RETINAL × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 BOG octyl beta-D-glucopyranoside × 2 PLM PALMITIC ACID × 1 SO4 SULFATE ION × 1 ACT ACETATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.85 Å R-free 0.250 |
| 3PQR Crystal structure of Metarhodopsin II in complex with a C-terminal peptide derived from the Galpha subunit of transducin Deposited 2010-11-26 | Assembly 2 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
340–350（11 aa）
Fragment：C-terminal peptide (UNP residues 340-350)
|
Mutation：K341L,C347V | RET RETINAL × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 BOG octyl beta-D-glucopyranoside × 4 PLM PALMITIC ACID × 2 SO4 SULFATE ION × 2 ACT ACETATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.85 Å R-free 0.250 |
| 3V00 Studies of a constitutively active G-alpha subunit provide insights into the mechanism of G protein activation. Deposited 2011-12-07 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
1–215（215 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain C
295–350（56 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
|
Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N | GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.3；295 K；1.9 M Ammonium Sulphate in 0.05 M Sodium Cacodylate buffer, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.272 |
| 3V00 Studies of a constitutively active G-alpha subunit provide insights into the mechanism of G protein activation. Deposited 2011-12-07 | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–215（215 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain B
295–350（56 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
|
Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N | GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.3；295 K；1.9 M Ammonium Sulphate in 0.05 M Sodium Cacodylate buffer, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.272 |
| 3V00 Studies of a constitutively active G-alpha subunit provide insights into the mechanism of G protein activation. Deposited 2011-12-07 | Assembly 3 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–215（215 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain A
295–350（56 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
|
Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N | GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.3；295 K；1.9 M Ammonium Sulphate in 0.05 M Sodium Cacodylate buffer, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.272 |
| 3V00 Studies of a constitutively active G-alpha subunit provide insights into the mechanism of G protein activation. Deposited 2011-12-07 | Assembly 4 Insufficient information Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
1–215（215 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain A
295–350（56 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain B
1–215（215 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain B
295–350（56 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain C
1–215（215 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain C
295–350（56 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
|
Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N | GDP GUANOSINE-5'-DIPHOSPHATE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.3；295 K；1.9 M Ammonium Sulphate in 0.05 M Sodium Cacodylate buffer, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.272 |
| 3V00 Studies of a constitutively active G-alpha subunit provide insights into the mechanism of G protein activation. Deposited 2011-12-07 | Assembly 5 Insufficient information Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
1–215（215 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain A
295–350（56 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain B
1–215（215 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain B
295–350（56 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain C
1–215（215 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain C
295–350（56 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
|
Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N | GDP GUANOSINE-5'-DIPHOSPHATE × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.3；295 K；1.9 M Ammonium Sulphate in 0.05 M Sodium Cacodylate buffer, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.272 |
| 3V00 Studies of a constitutively active G-alpha subunit provide insights into the mechanism of G protein activation. Deposited 2011-12-07 | Assembly 6 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–215（215 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain A
295–350（56 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain C
1–215（215 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain C
295–350（56 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
|
Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N | GDP GUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.3；295 K；1.9 M Ammonium Sulphate in 0.05 M Sodium Cacodylate buffer, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.272 |
| 3V00 Studies of a constitutively active G-alpha subunit provide insights into the mechanism of G protein activation. Deposited 2011-12-07 | Assembly 7 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–215（215 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
Chain B
295–350（56 aa）
Fragment：UNP P04695 1-215 & 295-350, UNP P10824 220-298
|
Mutation：G56P, K244H and D247N Mutation：G56P, K244H and D247N | GDP GUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.3；295 K；1.9 M Ammonium Sulphate in 0.05 M Sodium Cacodylate buffer, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.90 Å R-free 0.272 |
| 4BEY Night blindness causing G90D rhodopsin in complex with GaCT2 peptide Deposited 2013-03-12 | Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
340–350（11 aa）
Fragment：RESIDUES 340-350
|
Mutation：YES | SO4 SULFATE ION × 1 ACT ACETATE ION × 1 PLM PALMITIC ACID × 1 BOG octyl beta-D-glucopyranoside × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.90 Å R-free 0.259 |
| 4J4Q Crystal structure of active conformation of GPCR opsin stabilized by octylglucoside Deposited 2013-02-07 | Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
340–350（11 aa）
Fragment：C-terminal derived peptide, UNP RESIDUES 340-350
|
Mutation：K341L, C347V | BOG octyl beta-D-glucopyranoside × 4 PLM PALMITIC ACID × 1 SO4 SULFATE ION × 2 ACT ACETATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；AMMONIUM SULFATE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.65 Å R-free 0.230 |
| 6OY9 Structure of the Rhodopsin-Transducin Complex Deposited 2019-05-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–201（201 aa）
|
Not recorded | RET RETINAL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6OYA Structure of the Rhodopsin-Transducin-Nanobody Complex Deposited 2019-05-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain A
1–201（201 aa）
|
Not recorded | RET RETINAL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 1 second before plunging; avoid light as much as possible.
|
Resolution 3.30 Å |
| 7JSN Structure of the Visual Signaling Complex between Transducin and Phosphodiesterase 6 Deposited 2020-08-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain E
1–215（215 aa）
Chain E
295–350（56 aa）
Chain F
1–215（215 aa）
Chain F
295–350（56 aa）
|
Mutation：R174C,Q200L Mutation：R174C,Q200L Mutation：R174C,Q200L Mutation：R174C,Q200L | ZN ZINC ION × 2 MG MAGNESIUM ION × 2 VDN 2-{2-ETHOXY-5-[(4-ETHYLPIPERAZIN-1-YL)SULFONYL]PHENYL}-5-METHYL-7-PROPYLIMIDAZO[5,1-F][1,2,4]TRIAZIN-4(1H)-ONE × 2 35G GUANOSINE-3',5'-MONOPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8；20 mM Tris pH 8.0, 5 mM MgCl2 and 1 uM vardenafil
cryo-EM vitrification conditions
Cryogen ETHANE
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Resolution 3.20 Å |