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Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：P05161 New Search
22PDB entries
40Biological assembly
45chain/assembly records
Main Difference Dimensions in This Set
Different construct Different mutation/modification Different assembly state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
1Z2M Crystal Structure of ISG15, the Interferon-Induced Ubiquitin Cross Reactive Protein Deposited 2005-03-08 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 0–154（155 aa）
Mutation：c78s OS4 OSMIUM 4+ ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；292 K；Tris-HCl buffer, PEG4K, MgCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K
Resolution 2.50 Å R-free 0.223
2HJ8 Solution NMR structure of the C-terminal domain of the interferon alpha-inducible ISG15 protein from Homo sapiens. Northeast Structural Genomics target HR2873B Deposited 2006-06-30 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 78–156（79 aa） Fragment：ISG15 C-terminal domain (8.9 kDa), Ubiquitin-like 2
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；293 K；Ionic strength (raw mmCIF value) 50 mM ammonium citrate, 5 mM CaCl2；Pressure ambient
NMR sample composition 0.81 mM U-13C,15N HR2873B, 50 mM ammonium citrate, 5 mM CaCl2, 1x protease inhibitor, 0.02% NaN3, pH 6.5, 5% D2O / 95% H2O | 5% D2O / 95% H2O
NMR sample composition 0.9 mM 5%-13C,U-15N HR2873B, 50 mM ammonium citrate, 5 mM CaCl2, 1x protease inhibitor, 0.02% NaN3, pH 6.5, 5% D2O / 95% H2O | 5% D2O / 95% H2O
Resolution not provided
3PHX OTU Domain of Crimean Congo Hemorrhagic Fever Virus in complex with ISG15 Deposited 2010-11-04 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain B 79–156（78 aa） Fragment：UNP residues 79-156
Not recorded ZN ZINC ION × 12 ACY ACETIC ACID × 2 NEH ETHANAMINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；10% PEG8K, 0.2M zinc acetate, 0.1M MES sodium salt, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.60 Å R-free 0.192
3PHX OTU Domain of Crimean Congo Hemorrhagic Fever Virus in complex with ISG15 Deposited 2010-11-04 Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain B 79–156（78 aa） Fragment：UNP residues 79-156
Not recorded ZN ZINC ION × 12 ACY ACETIC ACID × 2 NEH ETHANAMINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；10% PEG8K, 0.2M zinc acetate, 0.1M MES sodium salt, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.60 Å R-free 0.192
3PSE Structure of a viral OTU domain protease bound to interferon-stimulated gene 15 (ISG15) Deposited 2010-12-01 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain B 1–156（156 aa）
Mutation：C78S 4LJ 1.7.6 3-bromanylpropan-1-amine × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；100 mM MES, 23% PEG6000, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.30 Å R-free 0.224
3R66 Crystal structure of human ISG15 in complex with NS1 N-terminal region from influenza virus B, Northeast Structural Genomics Consortium Target IDs HX6481, HR2873, and OR2 Deposited 2011-03-21 Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count
Chain C 1–157（157 aa）
Chain D 1–157（157 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；15% PEG3350, 1% dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.30 Å R-free 0.265
3RT3 Complex of influenza virus protein with host anti-viral factor Deposited 2011-05-03 Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count
Chain B 1–158（158 aa）
Mutation：C78S SIN SUCCINIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；20% PEG 8000, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 2.01 Å R-free 0.243
3SDL Crystal structure of human ISG15 in complex with NS1 N-terminal region from influenza B virus, Northeast Structural Genomics Consortium Target IDs HX6481, HR2873, and OR2 Deposited 2011-06-09 Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count
Chain C 1–157（157 aa）
Chain D 1–157（157 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；pH 7.5；293 K；15% PEG3350, 1% dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K, EVAPORATION
Resolution 2.29 Å R-free 0.261
5TL6 Crystal structure of SARS-CoV papain-like protease in complex with the C-terminal domain of human ISG15 Deposited 2016-10-10 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 80–157（78 aa） Fragment：C-terminal domain (UNP residues 80-157)
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1 M lithium sulfate, 0.1 M Bis-tris [pH 6.5], 22% PEG 3350, supplemented with 30% (v/v) glycerol additive in a 1:5 dilution
Resolution 2.62 Å R-free 0.250
5TL6 Crystal structure of SARS-CoV papain-like protease in complex with the C-terminal domain of human ISG15 Deposited 2016-10-10 Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain C 80–157（78 aa） Fragment：C-terminal domain (UNP residues 80-157)
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1 M lithium sulfate, 0.1 M Bis-tris [pH 6.5], 22% PEG 3350, supplemented with 30% (v/v) glycerol additive in a 1:5 dilution
Resolution 2.62 Å R-free 0.250
5W8T Crystal structure of MERS-CoV papain-like protease in complex with the C-terminal domain of human ISG15 Deposited 2017-06-22 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain B 80–156（77 aa）
Not recorded AYE prop-2-en-1-amine × 1 ZN ZINC ION × 5 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；200 mM CaCl2, 100 mM NaO2C2H3 pH 4.6, 28% (v/v) MPD
Resolution 2.76 Å R-free 0.224
5W8T Crystal structure of MERS-CoV papain-like protease in complex with the C-terminal domain of human ISG15 Deposited 2017-06-22 Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain D 80–156（77 aa）
Not recorded AYE prop-2-en-1-amine × 1 ZN ZINC ION × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；200 mM CaCl2, 100 mM NaO2C2H3 pH 4.6, 28% (v/v) MPD
Resolution 2.76 Å R-free 0.224
5W8U Crystal structure of MERS-CoV papain-like protease in complex with the C-terminal domain of human ISG15 Deposited 2017-06-22 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain B 80–156（77 aa） Fragment：UNP residues 80-156
Not recorded ZN ZINC ION × 3 AYE prop-2-en-1-amine × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；200 mM CaCl2, 100 mM NaO2C2H3 pH 4.6, 28% (v/v) MPD
Resolution 2.41 Å R-free 0.237
5W8U Crystal structure of MERS-CoV papain-like protease in complex with the C-terminal domain of human ISG15 Deposited 2017-06-22 Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain D 80–156（77 aa） Fragment：UNP residues 80-156
Not recorded ZN ZINC ION × 3 AYE prop-2-en-1-amine × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；200 mM CaCl2, 100 mM NaO2C2H3 pH 4.6, 28% (v/v) MPD
Resolution 2.41 Å R-free 0.237
6BI8 X-ray structure of MERS coronavirus papain-like protease in complex with human ISG15 Deposited 2017-11-01 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain C 1–156（156 aa）
Mutation：C78S PGE TRIETHYLENE GLYCOL × 2 ZN ZINC ION × 1 GOL GLYCEROL × 2 AYE prop-2-en-1-amine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.3；293.15 K；0.25 M Potassium Citrate, pH 8.3 20% PEG3350
Resolution 2.29 Å R-free 0.215
6BI8 X-ray structure of MERS coronavirus papain-like protease in complex with human ISG15 Deposited 2017-11-01 Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain D 1–156（156 aa）
Mutation：C78S PGE TRIETHYLENE GLYCOL × 2 ZN ZINC ION × 1 GOL GLYCEROL × 3 AYE prop-2-en-1-amine × 1 CIT CITRIC ACID × 1 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.3；293.15 K；0.25 M Potassium Citrate, pH 8.3 20% PEG3350
Resolution 2.29 Å R-free 0.215
6FFA FMDV Leader protease bound to substrate ISG15 Deposited 2018-01-05 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain B 79–155（77 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) GOL GLYCEROL × 5 SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；2 M Ammonium Sulfate 0.2 M Sodium potassium tartrate tetrahydrate 0.1 M Sodium citrate tribasic (pH 5.6)
Resolution 1.50 Å R-free 0.186
6XA9 SARS CoV-2 PLpro in complex with ISG15 C-terminal domain propargylamide Deposited 2020-06-04 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain B 79–157（79 aa） Fragment：C-terminal domain (UNP residues 79-157)
Mutation：G176 replaced with propargylamide Non-standard monomer：Yes (specific site not provided by mmCIF) GOL GLYCEROL × 3 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.2 M lithium sulfate, 25% PEG3350, 0.1 M Bis-Tris chloride, pH 6.5
Resolution 2.90 Å R-free 0.231
6XA9 SARS CoV-2 PLpro in complex with ISG15 C-terminal domain propargylamide Deposited 2020-06-04 Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain D 79–157（79 aa） Fragment：C-terminal domain (UNP residues 79-157)
Mutation：G176 replaced with propargylamide Non-standard monomer：Yes (specific site not provided by mmCIF) GOL GLYCEROL × 3 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.2 M lithium sulfate, 25% PEG3350, 0.1 M Bis-Tris chloride, pH 6.5
Resolution 2.90 Å R-free 0.231
6XA9 SARS CoV-2 PLpro in complex with ISG15 C-terminal domain propargylamide Deposited 2020-06-04 Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain F 79–157（79 aa） Fragment：C-terminal domain (UNP residues 79-157)
Mutation：G176 replaced with propargylamide Non-standard monomer：Yes (specific site not provided by mmCIF) GOL GLYCEROL × 2 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.2 M lithium sulfate, 25% PEG3350, 0.1 M Bis-Tris chloride, pH 6.5
Resolution 2.90 Å R-free 0.231
7RBS The crystal structure of Papain-Like Protease of SARS CoV-2, C111S mutant, in complex with human ISG15 Deposited 2021-07-06 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain B 2–157（156 aa）
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；289 K；0.02 M MgCl2, 0.1 M HEPES buffer, 22% poly(acrylicacid sodium salt) 5100
Resolution 2.98 Å R-free 0.236
7RBS The crystal structure of Papain-Like Protease of SARS CoV-2, C111S mutant, in complex with human ISG15 Deposited 2021-07-06 Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain D 2–157（156 aa）
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；289 K；0.02 M MgCl2, 0.1 M HEPES buffer, 22% poly(acrylicacid sodium salt) 5100
Resolution 2.98 Å R-free 0.236
7RBS The crystal structure of Papain-Like Protease of SARS CoV-2, C111S mutant, in complex with human ISG15 Deposited 2021-07-06 Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain F 2–157（156 aa）
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；289 K；0.02 M MgCl2, 0.1 M HEPES buffer, 22% poly(acrylicacid sodium salt) 5100
Resolution 2.98 Å R-free 0.236
7RBS The crystal structure of Papain-Like Protease of SARS CoV-2, C111S mutant, in complex with human ISG15 Deposited 2021-07-06 Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain H 2–157（156 aa）
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；289 K；0.02 M MgCl2, 0.1 M HEPES buffer, 22% poly(acrylicacid sodium salt) 5100
Resolution 2.98 Å R-free 0.236
7RBS The crystal structure of Papain-Like Protease of SARS CoV-2, C111S mutant, in complex with human ISG15 Deposited 2021-07-06 Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain J 2–157（156 aa）
Not recorded ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；289 K；0.02 M MgCl2, 0.1 M HEPES buffer, 22% poly(acrylicacid sodium salt) 5100
Resolution 2.98 Å R-free 0.236
7S6P The crystal structure of human ISG15 Deposited 2021-09-14 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 2–157（156 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；289 K；0.04 M potassium phosphate, 16% PEG8000, 20% glycerol
Resolution 2.15 Å R-free 0.268
7S6P The crystal structure of human ISG15 Deposited 2021-09-14 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 2–157（156 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；289 K；0.04 M potassium phosphate, 16% PEG8000, 20% glycerol
Resolution 2.15 Å R-free 0.268
7S6P The crystal structure of human ISG15 Deposited 2021-09-14 Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain C 2–157（156 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；289 K；0.04 M potassium phosphate, 16% PEG8000, 20% glycerol
Resolution 2.15 Å R-free 0.268
7S6P The crystal structure of human ISG15 Deposited 2021-09-14 Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain D 2–157（156 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；289 K；0.04 M potassium phosphate, 16% PEG8000, 20% glycerol
Resolution 2.15 Å R-free 0.268
7S6P The crystal structure of human ISG15 Deposited 2021-09-14 Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain E 2–157（156 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；289 K；0.04 M potassium phosphate, 16% PEG8000, 20% glycerol
Resolution 2.15 Å R-free 0.268
7S6P The crystal structure of human ISG15 Deposited 2021-09-14 Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain F 2–157（156 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；289 K；0.04 M potassium phosphate, 16% PEG8000, 20% glycerol
Resolution 2.15 Å R-free 0.268
8OIF Structure of the UBE1L activating enzyme bound to ISG15 and UBE2L6 Deposited 2023-03-22 Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count
Chain I 1–157（157 aa）
Not recorded AMP ADENOSINE MONOPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8SE9 Cryo-EM structure of a double loaded human UBA7-UBE2L6-ISG15 thioester mimetic complex (Form 2) Deposited 2023-04-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count
Chain B 1–157（157 aa）
Chain D 1–157（157 aa）
Mutation：C78S Mutation：C78S AMP ADENOSINE MONOPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8SEA Cryo-EM structure of a double loaded human UBA7-UBE2L6-ISG15 thioester mimetic complex (Form 1) Deposited 2023-04-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count
Chain B 1–157（157 aa）
Chain D 1–157（157 aa）
Mutation：C78S Mutation：C78S AMP ADENOSINE MONOPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8SEB Cryo-EM structure of a single loaded human UBA7-UBE2L6-ISG15 adenylate complex Deposited 2023-04-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count
Chain B 1–157（157 aa）
Mutation：C78S AMP ADENOSINE MONOPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.24 Å
8SV8 Cryo-EM structure of a double loaded human UBA7-UBE2L6-ISG15 thioester mimetic complex from a composite map Deposited 2023-05-15 Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count
Chain B 1–157（157 aa）
Chain D 1–157（157 aa）
Mutation：C78S Mutation：C78S AMP ADENOSINE MONOPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.38 Å
9NN9 ISG15 complexed with nanobody Deposited 2025-03-05 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–157（157 aa） Fragment：residues 1-157
Mutation：C78S EDO 1,2-ETHANEDIOL × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；292 K；25 % PEG 3350, 0.1 M trisodium citrate pH 5.6, 0.2 M ammonium sulfate
Resolution 2.59 Å R-free 0.244
9NN9 ISG15 complexed with nanobody Deposited 2025-03-05 Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain C 1–157（157 aa） Fragment：residues 1-157
Mutation：C78S EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；292 K；25 % PEG 3350, 0.1 M trisodium citrate pH 5.6, 0.2 M ammonium sulfate
Resolution 2.59 Å R-free 0.244
9NN9 ISG15 complexed with nanobody Deposited 2025-03-05 Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain E 1–157（157 aa） Fragment：residues 1-157
Mutation：C78S EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；292 K；25 % PEG 3350, 0.1 M trisodium citrate pH 5.6, 0.2 M ammonium sulfate
Resolution 2.59 Å R-free 0.244
9ZFO Cryo-EM Structure of Human STAT2-USP18-ISG15 Complex Deposited 2025-12-01 Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count
Chain I 1–157（157 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；25mM HEPES (pH 8.0), 200mM NaCl and 1.0mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.05 Å

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.