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Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：P05725 New Search
28PDB entries
31Biological assembly
61chain/assembly records
Main Difference Dimensions in This Set
Different construct Different mutation/modification Different assembly state Different ligand/ion Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
1AF5 GROUP I MOBILE INTRON ENDONUCLEASE Deposited 1997-03-21 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 4–138（135 aa）
Mutation：D56G No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 5；PROTEIN WAS CRYSTALLIZED FROM 23% PEG 6000, 0.1M CITRATE,PH 5.0
Resolution 3.00 Å R-free 0.373
1BP7 GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA Deposited 1998-08-13 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Not recorded CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；pH 6.5
Resolution 3.00 Å R-free 0.281
1BP7 GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA Deposited 1998-08-13 Assembly 2 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain C 2–153（152 aa）
Chain D 2–153（152 aa）
Not recorded CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；pH 6.5
Resolution 3.00 Å R-free 0.281
1G9Y HOMING ENDONUCLEASE I-CREI / DNA SUBSTRATE COMPLEX WITH CALCIUM Deposited 2000-11-28 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；25% PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP
Resolution 2.05 Å R-free 0.260
1G9Z LAGLIDADG HOMING ENDONUCLEASE I-CREI / DNA PRODUCT COMPLEX WITH MAGNESIUM Deposited 2000-11-28 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric（6） Consistent with all polymers
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Not recorded MG MAGNESIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；30% PEG400, PH 6.5, VAPOR DIFFUSION, HANGING DROP
Resolution 1.80 Å R-free 0.249
1N3E Crystal structure of I-CreI bound to a palindromic DNA sequence I (palindrome of left side of wildtype DNA target sequence) Deposited 2002-10-28 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric（6） Consistent with all polymers
Chain A 1–163（163 aa）
Chain B 1–163（163 aa）
Not recorded CA CALCIUM ION × 3 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；25% PEG 400, 20 mM NaCl, 10 mM CaCl2, 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.50 Å R-free 0.245
1N3E Crystal structure of I-CreI bound to a palindromic DNA sequence I (palindrome of left side of wildtype DNA target sequence) Deposited 2002-10-28 Assembly 2 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric（6） Consistent with all polymers
Chain G 1–163（163 aa）
Chain H 1–163（163 aa）
Not recorded CA CALCIUM ION × 3 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；25% PEG 400, 20 mM NaCl, 10 mM CaCl2, 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.50 Å R-free 0.245
1N3F Crystal structure of I-CreI bound to a palindromic DNA sequence II (palindrome of right side of wildtype DNA target sequence) Deposited 2002-10-28 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric（6） Consistent with all polymers
Chain A 1–163（163 aa）
Chain B 1–163（163 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；25% PEG 400, 20 mM NaCl, 10 mM CaCl2, 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.00 Å R-free 0.228
1N3F Crystal structure of I-CreI bound to a palindromic DNA sequence II (palindrome of right side of wildtype DNA target sequence) Deposited 2002-10-28 Assembly 2 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric（6） Consistent with all polymers
Chain G 1–163（163 aa）
Chain H 1–163（163 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；25% PEG 400, 20 mM NaCl, 10 mM CaCl2, 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.00 Å R-free 0.228
1T9I I-CreI(D20N)/DNA complex Deposited 2004-05-17 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain A 1–163（163 aa）
Chain B 1–163（163 aa）
Mutation：D20N Mutation：D20N CA CALCIUM ION × 2 NA SODIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；PEG400, sodium chloride, calcium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP
Resolution 1.60 Å R-free 0.211
1T9J I-CreI(Q47E)/DNA complex Deposited 2004-05-17 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain A 1–163（163 aa）
Chain B 1–163（163 aa）
Mutation：Q47E Mutation：Q47E No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；PEG 400, sodium chloride, calcium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP
Resolution 2.00 Å R-free 0.268
1U0C Y33C Mutant of Homing endonuclease I-CreI Deposited 2004-07-13 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain A 1–163（163 aa）
Chain B 1–163（163 aa）
Not recorded MG MAGNESIUM ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å R-free 0.277
1U0D Y33H Mutant of Homing endonuclease I-CreI Deposited 2004-07-13 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain A 1–163（163 aa）
Chain B 1–163（163 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.90 Å R-free 0.257
2I3P K28R mutant of Homing Endonuclease I-CreI Deposited 2006-08-20 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain A 1–153（153 aa）
Chain B 1–153（153 aa）
Mutation：K28R Mutation：K28R CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.5；298 K；PEG 400 28%, pH 6.5, VAPOR DIFFUSION, temperature 298K
Resolution 2.30 Å R-free 0.292
2I3Q Q44V mutant of Homing Endonuclease I-CreI Deposited 2006-08-20 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain A 1–153（153 aa）
Chain B 1–153（153 aa）
Mutation：Q44V Mutation：Q44V CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.5；298 K；PEG 400 28%, pH 6.5, VAPOR DIFFUSION, temperature 298K
Resolution 2.30 Å R-free 0.264
2O7M The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and cleavage. Identification of a novel site for specificity engineering in the I-CreI scaffold Deposited 2006-12-11 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–156（156 aa） Fragment：residues 1-156
Chain B 1–156（156 aa） Fragment：residues 1-156
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.00 Å R-free 0.235
2VBJ Molecular basis of human XPC gene recognition and cleavage by engineered homing endonuclease heterodimers Deposited 2007-09-14 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain A 2–153（152 aa） Fragment：RESIDUES 2-153
Chain B 2–153（152 aa） Fragment：RESIDUES 2-153
Not recorded CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；HANGING-DROP DNA-PROTEIN COMPLEX SOLUTION WAS 4 MG/ML. 35% 2-ETHOXYETHANOL IN 0.1M NA-CACODYLATE PH6.5
Resolution 1.95 Å R-free 0.216
2VBL Molecular basis of human XPC gene recognition and cleavage by engineered homing endonuclease heterodimers Deposited 2007-09-14 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric（6） Consistent with all polymers
Chain A 1–153（153 aa） Fragment：RESIDUES 1-153
Chain B 1–153（153 aa） Fragment：RESIDUES 1-153
Not recorded MG MAGNESIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；4 MG/ML PROTEIN, 35% METHANOL, 0.1M NACACODYLATE PH 6.5
Resolution 1.80 Å R-free 0.197
2VBN Molecular basis of human XPC gene recognition and cleavage by engineered homing endonuclease heterodimers Deposited 2007-09-14 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric（6） Consistent with all polymers
Chain A 1–153（153 aa） Fragment：RESIDUES 1-153
Chain B 1–153（153 aa） Fragment：RESIDUES 1-153
Not recorded MG MAGNESIUM ION × 3 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；4 MG/ML PROTEIN, 20% PEG1000, 0.1M IMIDAZOLE PH 8.0, 0.2 M CAAC2
Resolution 1.90 Å R-free 0.223
2VBO Molecular basis of human XPC gene recognition and cleavage by engineered homing endonuclease heterodimers Deposited 2007-09-14 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain A 1–153（153 aa） Fragment：RESIDUES 1-153
Chain B 1–153（153 aa） Fragment：RESIDUES 1-153
Not recorded CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.5；pH 4.5
Resolution 1.80 Å R-free 0.225
4AAB Crystal structure of the mutant D75N I-CreI in complex with its wild- type target (The four central bases, 2NN region, are composed by GTAC from 5' to 3') Deposited 2011-12-01 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric（6） Consistent with all polymers
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Mutation：YES Mutation：YES PGO S-1,2-PROPANEDIOL × 5 MG MAGNESIUM ION × 3 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；30% PROPANEDIOL, 0.1M HEPES PH 7.5, 20% PEG400
Resolution 2.50 Å R-free 0.225
4AAD Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in absence of metal ions at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3') Deposited 2011-12-01 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Mutation：YES Mutation：YES GOL GLYCEROL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；20% PEG300, 0.1M TRIS PH 8.5, 5% PEG8000, 10% GLYCEROL
Resolution 3.10 Å R-free 0.238
4AAE Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by AGCG from 5' to 3') Deposited 2011-12-01 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain A 2–154（153 aa）
Chain B 2–154（153 aa）
Mutation：YES Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 9.5；50% PEG400, 0.1M CHES PH 9.5, 0.2M NACL
Resolution 2.60 Å R-free 0.242
4AAF Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3') Deposited 2011-12-01 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Mutation：YES Mutation：YES EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；35% METOH, 0.1M SODIUM CACODYLATE PH 6.5,
Resolution 2.50 Å R-free 0.247
4AAG Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in presence of Ca at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3') Deposited 2011-12-01 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Mutation：YES Mutation：YES CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；30% PEG400, 0.1M HEPES PH 7.5, 0.1M NACL
Resolution 2.80 Å R-free 0.250
4AQU Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of calcium Deposited 2012-04-19 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain A 2–153（152 aa） Fragment：RESIDUES 2-153
Chain B 2–153（152 aa） Fragment：RESIDUES 2-153
Not recorded CA CALCIUM ION × 2 GOL GLYCEROL × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.30 Å R-free 0.249
4AQX Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of magnesium Deposited 2012-04-19 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric（6） Consistent with all polymers
Chain A 2–153（152 aa） Fragment：RESIDUES 2-153
Chain B 2–153（152 aa） Fragment：RESIDUES 2-153
Not recorded GOL GLYCEROL × 5 MG MAGNESIUM ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.20 Å R-free 0.242
6FB2 Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with its target DNA (Haemoglobin beta subunit gene) in the presence of Manganese Deposited 2017-12-18 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric（6） Consistent with all polymers
Chain A 2–154（153 aa）
Chain B 2–155（154 aa）
Not recorded MN MANGANESE (II) ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M calcium acetate, 0.1 M sodium acetate pH 4.6-5.4, 33-40% (v/v) 1,2-propanediol
Resolution 2.95 Å R-free 0.235
6FB7 Crystal Structure of the I-CreI Homing Endonuclease D75N variant in complex with its target DNA in the presence of Manganese Deposited 2017-12-18 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric（6） Consistent with all polymers
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Not recorded MN MANGANESE (II) ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M calcium acetate, 0.1 M sodium acetate pH 4.6-5.4, 33-40% (v/v) 1,2-propanediol
Resolution 2.69 Å R-free 0.211
6FB8 Crystal Structure of the I-CreI Homing Endonuclease D75N variant in complex with an altered version of its target DNA at 5NNN region in the presence of Magnesium Deposited 2017-12-18 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Not recorded MG MAGNESIUM ION × 4 PGO S-1,2-PROPANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M calcium acetate, 0.1 M sodium acetate pH 4.6-5.4, 33-40% (v/v) 1,2-propanediol
Resolution 2.45 Å R-free 0.224
6FB9 Crystal Structure of the I-CreI Homing Endonuclease D75N variant in complex with an altered version of its target DNA at 5NNN region in the presence of Manganese Deposited 2017-12-18 Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric（6） Consistent with all polymers
Chain A 2–153（152 aa）
Chain B 2–153（152 aa）
Not recorded MN MANGANESE (II) ION × 5 PGO S-1,2-PROPANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M calcium acetate, 0.1 M sodium acetate pH 4.6-5.4, 33-40% (v/v) 1,2-propanediol
Resolution 2.95 Å R-free 0.203

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.