当前蛋白身份：P07617
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1AV6 VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M7G CAPPED RNA HEXAMER AND S-ADENOSYLHOMOCYSTEINE 提交 1997-09-26 | Assembly 1 蛋白–RNA 单体；蛋白 × 1 PDB 声明：dimeric（2） 与全部聚合物一致 |
链 A
3–297（295 aa）
|
突变：C-TERMINAL DELETION OF 26 RESIDUES | MGT 7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；pH 8.50
|
分辨率 2.80 Å R-free 0.285 |
| 1B42 VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M1ADE AND S-ADENOSYLHOMOCYSTEINE 提交 1999-01-05 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–297（297 aa）
|
突变：C-TERMINAL DELETION OF 26 RESIDUES | SAH S-ADENOSYL-L-HOMOCYSTEINE × 2 M1A 6-AMINO-1-METHYLPURINE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；pH 6.5
|
分辨率 2.20 Å R-free 0.256 |
| 1BKY VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M1CYT AND S-ADENOSYLHOMOCYSTEINE 提交 1998-07-13 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–307（307 aa）
|
突变：C-TERMINAL DELETION OF 26 RESIDUES | SAH S-ADENOSYL-L-HOMOCYSTEINE × 2 1MC 1-METHYLCYTOSINE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；pH 6.5
|
分辨率 2.00 Å R-free 0.261 |
| 1EAM VACCINIA METHYLTRANSFERASE VP39 MUTANT (EC: 2.7.7.19) 提交 1999-01-26 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–307（307 aa）
|
突变：C-TERMINAL DELETION OF 26 RESIDUES & E233A | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；pH 6.5
|
分辨率 2.00 Å R-free 0.268 |
| 1EQA VACCINIA METHYLTRANSFERASE VP39 MUTANT E233Q COMPLEXED WITH M7G AND S-ADENOSYLHOMOCYSTEINE 提交 1999-01-05 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–297（297 aa）
|
突变：C-TERMINAL DELETION OF 26 RESIDUES | SAH S-ADENOSYL-L-HOMOCYSTEINE × 2 MG7 7-METHYLGUANOSINE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；pH 6.5
|
分辨率 2.20 Å R-free 0.255 |
| 1JSZ Crystal Structure Analysis of N7,9-dimethylguanine-VP39 complex 提交 2001-08-19 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–307（307 aa）
片段：residues 1-307
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 NDM 7,9-DIMETHYLGUANINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；PEG 8000, sodium citrate, ammonium sulphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 1.93 Å R-free 0.252 |
| 1JTE Crystal Structure Analysis of VP39 F180W mutant 提交 2001-08-20 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–307（307 aa）
片段：residues 1-307
|
突变：F180W | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；PEG 8000, sodium citrate, ammonium sulphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
分辨率 2.00 Å R-free 0.285 |
| 1JTF Crystal Structure Analysis of VP39-F180W mutant and m7GpppG complex 提交 2001-08-20 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–307（307 aa）
片段：residues 1-307
|
突变：F180W | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 M7G 7N-METHYL-8-HYDROGUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；PEG 8000, sodium citrate, ammonium sulphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.60 Å R-free 0.279 |
| 1P39 DC26 MUTANT OF VACCINIA VIRUS PROTEIN VP39 IN COMPLEX WITH S-ADENOSYLHOMOCYSTEINE AND M7G(5')PPPG 提交 1996-12-16 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–307（307 aa）
|
突变：26 C-TERMINAL RESIDUES DELETED | SAH S-ADENOSYL-L-HOMOCYSTEINE × 2 MGT 7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 4.5；DC26 CRYSTALLIZED IN 2MM ADOHCY AND 2MM M7GPPPG., pH 4.5
|
分辨率 2.00 Å |
| 1V39 DC26 MUTANT OF VACCINIA VIRUS PROTEIN VP39 IN COMPLEX WITH S-ADENOSYLHOMOCYSTEINE AND M7G(5')PPPG 提交 1996-12-16 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–307（307 aa）
|
突变：26 C-TERMINAL RESIDUES DELETED | SAH S-ADENOSYL-L-HOMOCYSTEINE × 2 M7G 7N-METHYL-8-HYDROGUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；DC26/ADOHCY CRYSTAL SOAKED IN 10MM M7GPPPG., pH 6.5
|
分辨率 1.80 Å |
| 1VP3 VACCINIA VIRUS PROTEIN VP39 IN COMPLEX WITH S-ADENOSYLHOMOCYSTEINE 提交 1996-11-21 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–333（333 aa）
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 4.5；pH 4.5
|
分辨率 1.90 Å |
| 1VP9 DC26 MUTANT OF VACCINIA VIRUS PROTEIN VP39 IN COMPLEX WITH S-ADENOSYLHOMOCYSTEINE 提交 1996-11-23 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–307（307 aa）
|
突变：26 C-TERMINAL RESIDUES DELETED | SAH S-ADENOSYL-L-HOMOCYSTEINE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；pH 6.5
|
分辨率 1.95 Å |
| 1VPT AS11 VARIANT OF VACCINIA VIRUS PROTEIN VP39 IN COMPLEX WITH S-ADENOSYL-L-METHIONINE 提交 1996-03-20 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–333（333 aa）
|
突变：AS11 VARIANT (R140A, K142A, R143A) | SAM S-ADENOSYLMETHIONINE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 4.5；pH 4.5
|
分辨率 1.80 Å R-free 0.250 |
| 2GA9 Crystal Structure of the Heterodimeric Vaccinia Virus Polyadenylate Polymerase with Bound ATP-gamma-S 提交 2006-03-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–297（297 aa）
|
突变：R140A K142A R143A | CA CALCIUM ION × 2 AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.7；293 K；20% PEG 4000, 0.240 M CaCl2, 5% glycerol, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.30 Å R-free 0.260 |
| 2GAF Crystal Structure of the Vaccinia Polyadenylate Polymerase Heterodimer (apo form) 提交 2006-03-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–297（297 aa）
|
突变：R140A, K142A, R143A 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.7；293 K；20% PEG 4000, 0.24 M CaCl2, 5% glycerol, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.40 Å R-free 0.288 |
| 2VP3 DC26 MUTANT OF VACCINIA VIRUS PROTEIN VP39 IN COMPLEX WITH S-ADENOSYLHOMOCYSTEINE AND M7G(5')PPPG 提交 1996-12-16 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–307（307 aa）
|
突变：26 C-TERMINAL RESIDUES DELETED | SAH S-ADENOSYL-L-HOMOCYSTEINE × 2 M7G 7N-METHYL-8-HYDROGUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；DC26/ADOHCY CRYSTAL SOAKED IN 10MM M7GDP., pH 6.5
|
分辨率 1.95 Å |
| 3ER8 Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase complex with two fragments of RNA 提交 2008-10-01 | Assembly 1 蛋白–DNA 异源复合物；蛋白 × 2 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 A
1–297（297 aa）
|
突变：R140A,K142A,R143A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.7；295 K；proteins(4-5mg.ml) in 10mM Tris-HCl, pH8.7, 75 mM NaCl, 0.5 mM DTT, mixed with equal volume buffer which composed of 10mM Tris-HCl, pH 8.7, 15-20% PEG 4000, 5% glycerol, 0.5 mM DTT. room temperature for several days., VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 3.18 Å R-free 0.285 |
| 3ER8 Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase complex with two fragments of RNA 提交 2008-10-01 | Assembly 2 蛋白–DNA 异源复合物；蛋白 × 2 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 B
1–297（297 aa）
|
突变：R140A,K142A,R143A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.7；295 K；proteins(4-5mg.ml) in 10mM Tris-HCl, pH8.7, 75 mM NaCl, 0.5 mM DTT, mixed with equal volume buffer which composed of 10mM Tris-HCl, pH 8.7, 15-20% PEG 4000, 5% glycerol, 0.5 mM DTT. room temperature for several days., VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 3.18 Å R-free 0.285 |
| 3ER9 Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase complex with UU and 3'-deoxy ATP 提交 2008-10-01 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 2 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–297（297 aa）
|
突变：R140A,K142A,R143A | GOL GLYCEROL × 3 3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.7；295 K；proteins(4-5mg.ml) in 10mM tris-HCl, pH 8.7, 75 mM NaCl, 0.5 mM DTT, mixed with equal volume buffer which composed of 10mM Tris-HCl, pH 8.7, 15-20% PEG 4000, 5% glycerol, 0.5 mM DTT. Room temperature for several days., VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 2.06 Å R-free 0.250 |
| 3ERC Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase with three fragments of RNA and 3'-deoxy ATP 提交 2008-10-01 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 2 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 A
1–297（297 aa）
|
突变：R140A,K142A,R143A | U5P URIDINE-5'-MONOPHOSPHATE × 1 3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.7；295 K；Proteins(4-5mg.ml) in 10mM Tris-HCl, pH8.7, 75 mM NaCl, 0.5 mM DTT, mixed with equal volume buffer which composed of 10mM Tris-HCl, pH 8.7, 15-20% PEG 4000, 5% glycerol, 0.5 mM DTT. Room temperature for several days., VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 3.21 Å R-free 0.309 |
| 3ERC Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase with three fragments of RNA and 3'-deoxy ATP 提交 2008-10-01 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 2 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 B
1–297（297 aa）
|
突变：R140A,K142A,R143A | U5P URIDINE-5'-MONOPHOSPHATE × 1 3AT 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE × 1 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.7；295 K；Proteins(4-5mg.ml) in 10mM Tris-HCl, pH8.7, 75 mM NaCl, 0.5 mM DTT, mixed with equal volume buffer which composed of 10mM Tris-HCl, pH 8.7, 15-20% PEG 4000, 5% glycerol, 0.5 mM DTT. Room temperature for several days., VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 3.21 Å R-free 0.309 |
| 3MAG VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M3ADE AND S-ADENOSYLHOMOCYSTEINE 提交 1998-07-12 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–307（307 aa）
|
突变：C-TERMINAL DELETION OF 26 RESIDUES | SAH S-ADENOSYL-L-HOMOCYSTEINE × 2 3MA 6-AMINO-3-METHYLPURINE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；pH 6.5
|
分辨率 1.80 Å R-free 0.251 |
| 3MCT VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M3CYT AND S-ADENOSYLHOMOCYSTEINE 提交 1999-01-05 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–297（297 aa）
|
突变：C-TERMINAL DELETION OF 26 RESIDUES | SAH S-ADENOSYL-L-HOMOCYSTEINE × 2 3MC 3-METHYLCYTOSINE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；pH 6.5
|
分辨率 2.00 Å R-free 0.285 |
| 4DCG VACCINIA METHYLTRANSFERASE VP39 MUTANT D182A COMPLEXED WITH M7G AND S-ADENOSYLHOMOCYSTEINE 提交 1998-07-13 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–307（307 aa）
|
突变：D182A, C-TERMINAL DELETION OF 26 RESIDUES | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 MG7 7-METHYLGUANOSINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；pH 6.5
|
分辨率 1.80 Å R-free 0.258 |