Current Protein Identity：P09211
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 10GS HUMAN GLUTATHIONE S-TRANSFERASE P1-1, COMPLEX WITH TER117 Deposited 1997-08-14 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | VWW L-gamma-glutamyl-S-benzyl-N-[(S)-carboxy(phenyl)methyl]-L-cysteinamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.8；pH 5.8
|
Resolution 2.20 Å R-free 0.214 |
| 11GS Glutathione s-transferase complexed with ethacrynic acid-glutathione conjugate (form ii) Deposited 1997-11-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | GSH Glutathione × 2 EAA ETHACRYNIC ACID × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.4；pH 6.4
|
Resolution 2.30 Å R-free 0.270 |
| 12GS GLUTATHIONE S-TRANSFERASE COMPLEXED WITH S-NONYL-GLUTATHIONE Deposited 1997-11-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | 0HH L-gamma-glutamyl-S-nonyl-L-cysteinylglycine × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.4；pH 5.4
|
Resolution 2.10 Å R-free 0.261 |
| 13GS GLUTATHIONE S-TRANSFERASE COMPLEXED WITH SULFASALAZINE Deposited 1997-11-20 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | GSH Glutathione × 2 SAS 2-HYDROXY-(5-([4-(2-PYRIDINYLAMINO)SULFONYL]PHENYL)AZO)BENZOIC ACID × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.4；pH 5.4
|
Resolution 1.90 Å R-free 0.222 |
| 14GS GLUTATHIONE S-TRANSFERASE P1-1 APO FORM 1 Deposited 1997-11-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.6；pH 6.6
|
Resolution 2.80 Å R-free 0.240 |
| 16GS GLUTATHIONE S-TRANSFERASE P1-1 APO FORM 3 Deposited 1997-11-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | SO4 SULFATE ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.8；pH 5.8
|
Resolution 1.90 Å R-free 0.263 |
| 17GS GLUTATHIONE S-TRANSFERASE P1-1 Deposited 1997-12-07 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Mutation：K54A Mutation：K54A | GTX S-HEXYLGLUTATHIONE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.6；pH 6.6
|
Resolution 1.90 Å R-free 0.200 |
| 18GS GLUTATHIONE S-TRANSFERASE P1-1 COMPLEXED WITH 1-(S-GLUTATHIONYL)-2,4-DINITROBENZENE Deposited 1997-12-07 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | GDN GLUTATHIONE S-(2,4 DINITROBENZENE) × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.4；pH 5.4
|
Resolution 1.90 Å R-free 0.238 |
| 19GS Glutathione s-transferase p1-1 Deposited 1997-12-14 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | BSP 3,3'-(4,5,6,7-TETRABROMO-3-OXO-1(3H)-ISOBENZOFURANYLIDENE)BIS [6-HYDROXYBENZENESULFONIC ACID]ANION × 2 GSH Glutathione × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
hanging drop；pH 6；CRYSTALLISATION PERFORMED USING THE HANGING DROP TECHNIQUE. 2 MICROL OF PROTEIN SOLUTION (8MG/ML) IN 10MM PHOSPHATE BUFFER PH7.0, 1MM EDTA AND 2MM BETA-MERCAPTOETHANOL WAS ADDED TO 2 MICROL OF RESERVOIR SOLUTION. THE RESERVOIR CONTAINED 20-25% AMMONIUM SULFATE, 30-60 MM DITHIOTHREITOL AND 100MM MORPHOLINOETHANESULFONIC ACID BUFFER PH5.4. DROPS WERE STREAK SEEDED AFTER 1 DAY USING A CAT'S WHISKER FROM CRYSTALS GROWN UNDER SIMILAR CONDITIONS. AFTER X DAYS, THE CRYSTAL WAS TRANSFERED TO ARTIFICIAL MOTHER LIQUOR WHICH LACKED DITHIOTHREITOL. SOLID BROMOSULFALEIN WAS SEEDED INTO THE DROP. SEVEN WEEKS LATER THE CRYSTALS WERE SUBJECTED TO X-RAY ANALYSIS., pH 6.0, hanging drop
|
Resolution 1.90 Å R-free 0.249 |
| 1AQV GLUTATHIONE S-TRANSFERASE IN COMPLEX WITH P-BROMOBENZYLGLUTATHIONE Deposited 1997-08-01 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | 0HG N-[(4S)-4-ammonio-4-carboxybutanoyl]-S-(4-bromobenzyl)-L-cysteinylglycine × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 1.94 Å R-free 0.252 |
| 1AQW GLUTATHIONE S-TRANSFERASE IN COMPLEX WITH GLUTATHIONE Deposited 1997-08-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | GSH Glutathione × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 1.80 Å R-free 0.261 |
| 1AQW GLUTATHIONE S-TRANSFERASE IN COMPLEX WITH GLUTATHIONE Deposited 1997-08-03 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1–209（209 aa）
Chain D
1–209（209 aa）
|
Not recorded | GSH Glutathione × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 1.80 Å R-free 0.261 |
| 1AQX GLUTATHIONE S-TRANSFERASE IN COMPLEX WITH MEISENHEIMER COMPLEX Deposited 1997-08-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | GTD 1-(S-GLUTATHIONYL)-2,4,6-TRINITROCYCLOHEXA-2,5-DIENE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.00 Å R-free 0.283 |
| 1AQX GLUTATHIONE S-TRANSFERASE IN COMPLEX WITH MEISENHEIMER COMPLEX Deposited 1997-08-03 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1–209（209 aa）
Chain D
1–209（209 aa）
|
Not recorded | GTD 1-(S-GLUTATHIONYL)-2,4,6-TRINITROCYCLOHEXA-2,5-DIENE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.00 Å R-free 0.283 |
| 1EOG CRYSTAL STRUCTURE OF PI CLASS GLUTATHIONE TRANSFERASE Deposited 2000-03-22 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
3–210（208 aa）
Chain B
3–210（208 aa）
|
Mutation：S149A Mutation：S149A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；25% ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K
|
Resolution 2.10 Å R-free 0.238 |
| 1EOH GLUTATHIONE TRANSFERASE P1-1 Deposited 2000-03-22 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–210（209 aa）
Chain B
2–210（209 aa）
|
Mutation：D152A Mutation：D152A | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.50 Å R-free 0.286 |
| 1EOH GLUTATHIONE TRANSFERASE P1-1 Deposited 2000-03-22 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
2–210（209 aa）
Chain D
2–210（209 aa）
|
Mutation：D152A Mutation：D152A | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.50 Å R-free 0.286 |
| 1EOH GLUTATHIONE TRANSFERASE P1-1 Deposited 2000-03-22 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
2–210（209 aa）
Chain F
2–210（209 aa）
|
Mutation：D152A Mutation：D152A | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.50 Å R-free 0.286 |
| 1EOH GLUTATHIONE TRANSFERASE P1-1 Deposited 2000-03-22 | Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
2–210（209 aa）
Chain H
2–210（209 aa）
|
Mutation：D152A Mutation：D152A | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.50 Å R-free 0.286 |
| 1GSS THREE-DIMENSIONAL STRUCTURE OF CLASS PI GLUTATHIONE S-TRANSFERASE FROM HUMAN PLACENTA IN COMPLEX WITH S-HEXYLGLUTATHIONE AT 2.8 ANGSTROMS RESOLUTION Deposited 1992-05-28 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | LEE L-gamma-glutamyl-S-hexyl-L-cysteinylglycine × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.80 Å |
| 1KBN Glutathione transferase mutant Deposited 2001-11-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Mutation：I148A,Y153A Mutation：I148A,Y153A | GSH Glutathione × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GOL GLYCEROL × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.00 Å R-free 0.244 |
| 1LBK Crystal structure of a recombinant glutathione transferase, created by replacing the last seven residues of each subunit of the human class pi isoenzyme with the additional C-terminal helix of human class alpha isoenzyme Deposited 2002-04-04 | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–202（202 aa）
Chain B
1–202（202 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GSH Glutathione × 2 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；PEG 8000, calcium chloride, glutathione, (R,R)-1,4-dithiothreitol, 2-[N-morpholino]ethanesulphonic acid, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.86 Å R-free 0.224 |
| 1MD3 A folding mutant of human class pi glutathione transferase, created by mutating glycine 146 of the wild-type protein to alanine Deposited 2002-08-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Mutation：G145A Mutation：G145A | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GSH Glutathione × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；295 K；MES, PEG 8000, calcium chloride, DTT(dithiothreitol), glutathione(reduced), pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.03 Å R-free 0.218 |
| 1MD4 A folding mutant of human class pi glutathione transferase, created by mutating glycine 146 of the wild-type protein to valine Deposited 2002-08-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Mutation：G145V Mutation：G145V | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GSH Glutathione × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；295 K；MES, PEG 8000, calcium chloride, DTT(dithiothreitol), glutathione(reduced), pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.10 Å R-free 0.235 |
| 1PGT CRYSTAL STRUCTURE OF HUMAN GLUTATHIONE S-TRANSFERASE P1-1[V104] COMPLEXED WITH S-HEXYLGLUTATHIONE Deposited 1997-02-17 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Mutation：VAL 104 VARIANT Mutation：VAL 104 VARIANT | GTX S-HEXYLGLUTATHIONE × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；CRYSTALS WERE GROWN IN HANGING DROPS WHICH INITIALLY CONSISTED OF 5.9 MG/ML PROTEIN IN 0.1 M HEPES BUFFER (PH 6.5) CONTAINING 8.3 MM S-HEXYLGLUTATHIONE AND 1.0 M BUFFERED (PH 6.5) AMMONIUM SULFATE. THE DROPS WERE EQUILIBRATED AT 293 K AGAINST WELL SOLUTION CONTAINING BETWEEN 1.9 - 2.0 M AMMONIUM SULFATE IN 0.1 M HEPES BUFFER (PH 6.5)., vapor diffusion - hanging drop
|
Resolution 1.80 Å |
| 1PX6 A folding mutant of human class pi glutathione transferase, created by mutating aspartate 153 of the wild-type protein to asparagine Deposited 2003-07-02 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Mutation：D152N Mutation：D152N | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GSH Glutathione × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；295 K；MES, PEG 8000, CALCIUM CHLORIDE, DTT(DITHIOTHREITOL), GLUTATHIONE(REDUCED), pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.10 Å R-free 0.235 |
| 1PX7 A folding mutant of human class pi glutathione transferase, created by mutating aspartate 153 of the wild-type protein to glutamate Deposited 2003-07-02 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Mutation：D152E Mutation：D152E | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GSH Glutathione × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；295 K；MES, PEG 8000, CALCIUM CHLORIDE, DTT(DITHIOTHREITOL), GLUTATHIONE(REDUCED), pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.03 Å R-free 0.218 |
| 1ZGN Crystal Structure of the Glutathione Transferase Pi in Complex with Dinitrosyl-diglutathionyl Iron Complex Deposited 2005-04-21 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | FE FE (III) ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GSH Glutathione × 2 NO NITRIC OXIDE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；295.15 K；MES, PEG 8000, Calcuim chloride, DTT, GSH, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K
|
Resolution 2.10 Å R-free 0.244 |
| 20GS GLUTATHIONE S-TRANSFERASE P1-1 COMPLEXED WITH CIBACRON BLUE Deposited 1997-12-16 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 CBD CIBACRON BLUE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
vapor diffusion - hanging drop - streak seeding；pH 6；CRYSTALLISATION PERFORMED USING THE HANGING DROP TECHNIQUE. 2 MICROL OF PROTEIN SOLUTION (8MG/ML) IN 10MM PHOSPHATE BUFFER PH7.0, 1MM EDTA AND 2MM BETA-MERCAPTOETHANOL WAS ADDED TO 2 MICROL OF RESERVOIR SOLUTION. THE RESERVOIR CONTAINED 20-25% AMMONIUM SULFATE, 30-60 MM DITHIOTHREITOL AND 100MM MORPHOLINOETHANESULFONIC ACID BUFFER PH5.4. DROPS WERE STREAK SEEDED AFTER 1 DAY USING A CAT'S WHISKER FROM CRYSTALS GROWN UNDER SIMILAR CONDITIONS. SOLID CIBACRON BLUE WAS SEEDED INTO THE DROP. SIX WEEKS LATER THE CRYSTALS WERE SUBJECTED TO X-RAY ANALYSIS., pH 6.0, vapor diffusion - hanging drop - streak seeding
|
Resolution 2.45 Å R-free 0.280 |
| 22GS HUMAN GLUTATHIONE S-TRANSFERASE P1-1 Y49F MUTANT Deposited 1998-03-10 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Mutation：Y49F Mutation：Y49F | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.4；pH 5.4
|
Resolution 1.90 Å R-free 0.230 |
| 2A2R Crystal Structure of Glutathione Transferase Pi in complex with S-nitrosoglutathione Deposited 2005-06-23 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GSN 2-AMINO-5-[1-(CARBOXYLATOMETHYLCARBAMOYL)-2-NITROSOSULFANYL-ETHYL]AMINO-5-OXO-PENTANOATE × 2 CA CALCIUM ION × 1 CO3 CARBONATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；295.15 K；MES, PEG 8000, Calcium Chloride, DTT, GSH, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K
|
Resolution 1.40 Å R-free 0.213 |
| 2A2S Crystal Structure of Human Glutathione Transferase in complex with S-nitrosoglutathione in the absence of reducing agent Deposited 2005-06-23 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GSN 2-AMINO-5-[1-(CARBOXYLATOMETHYLCARBAMOYL)-2-NITROSOSULFANYL-ETHYL]AMINO-5-OXO-PENTANOATE × 2 CA CALCIUM ION × 1 CO3 CARBONATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；295.15 K；MES, PEG 8000, Calcium chloride, GSNO, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K
|
Resolution 1.70 Å R-free 0.208 |
| 2GSS HUMAN GLUTATHIONE S-TRANSFERASE P1-1 IN COMPLEX WITH ETHACRYNIC ACID Deposited 1996-10-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | SO4 SULFATE ION × 1 EAA ETHACRYNIC ACID × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.4；pH 5.4
|
Resolution 1.90 Å R-free 0.229 |
| 2J9H Crystal structure of human glutathione-S-transferase P1-1 cys-free mutant in complex with S-hexylglutathione at 2.4 A resolution Deposited 2006-11-08 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–210（209 aa）
Chain B
2–210（209 aa）
|
Mutation：YES Mutation：YES | GTX S-HEXYLGLUTATHIONE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；SITTING DROP VAPOUR TECHNIQUE, 1 MICROLITRE OF PROTEIN AT 10 MG/ML IN 20 MM TRIS-HCL, PH 8.0 CONTAINING 5MM S-HEXYLGLUTATHIONE WAS MIXED WITH 1 MICROLITRE OF RESERVOIR SOLUTION, COMPOSED OF 1.5M AMMONIUM SULFATE AND 100MM SODIUM CITRATE
|
Resolution 2.40 Å R-free 0.310 |
| 2PGT CRYSTAL STRUCTURE OF HUMAN GLUTATHIONE S-TRANSFERASE P1-1[V104] COMPLEXED WITH (9R,10R)-9-(S-GLUTATHIONYL)-10-HYDROXY-9,10-DIHYDROPHENANTHRENE Deposited 1997-02-17 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Mutation：VAL 104 VARIANT Mutation：VAL 104 VARIANT | SO4 SULFATE ION × 1 GPR (9R,10R)-9-(S-GLUTATHIONYL)-10-HYDROXY-9,10-DIHYDROPHENANTHRENE × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；CRYSTALS WERE GROWN IN HANGING DROPS WHICH INITIALLY CONSISTED OF 5.9 MG/ML PROTEIN IN 0.1 M HEPES BUFFER (PH 7.0) CONTAINING 8.3 MM S-HEXYLGLUTATHIONE AND 1.0 M BUFFERED (PH 7.0) AMMONIUM SULFATE. THE DROPS WERE EQUILIBRATED AT 293 K AGAINST WELL SOLUTION CONTAINING BETWEEN 1.9 - 2.0 M AMMONIUM SULFATE IN 0.1 M HEPES BUFFER (PH 7.0)., vapor diffusion - hanging drop
|
Resolution 1.90 Å |
| 3CSH Crystal Structure of Glutathione Transferase Pi in complex with the Chlorambucil-Glutathione Conjugate Deposited 2008-04-09 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–210（209 aa）
Chain B
2–210（209 aa）
|
Not recorded | CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 LZ6 L-gamma-glutamyl-S-(2-{[4-(3-carboxypropyl)phenyl](2-chloroethyl)amino}ethyl)-L-cysteinylglycine × 2 GSH Glutathione × 2 CA CALCIUM ION × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；295 K；Solid chlorambucil was added to preformed GST crystals cocrystallised with glutathione , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.55 Å R-free 0.200 |
| 3CSI Crystal Structure of the Glutathione Transferase Pi allelic variant*C, I104V/A113V, in complex with the Chlorambucil-Glutathione Conjugate Deposited 2008-04-09 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
2–210（209 aa）
Chain D
2–210（209 aa）
|
Mutation：I105V, A114V Mutation：I105V, A114V | CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 LZ6 L-gamma-glutamyl-S-(2-{[4-(3-carboxypropyl)phenyl](2-chloroethyl)amino}ethyl)-L-cysteinylglycine × 2 GSH Glutathione × 2 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；Resevior solution contained: 100mM MES, 22% PEG 8000, 20mM CaCl2, 10mM DTT and 10mM GSH.
Soaking conditions as above, minus DTT, plus 2mM Chlorambucil dissolved in ethanol., pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.90 Å R-free 0.222 |
| 3CSI Crystal Structure of the Glutathione Transferase Pi allelic variant*C, I104V/A113V, in complex with the Chlorambucil-Glutathione Conjugate Deposited 2008-04-09 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–210（209 aa）
Chain B
2–210（209 aa）
|
Mutation：I105V, A114V Mutation：I105V, A114V | CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 LZ6 L-gamma-glutamyl-S-(2-{[4-(3-carboxypropyl)phenyl](2-chloroethyl)amino}ethyl)-L-cysteinylglycine × 2 GSH Glutathione × 2 CO3 CARBONATE ION × 1 CA CALCIUM ION × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；Resevior solution contained: 100mM MES, 22% PEG 8000, 20mM CaCl2, 10mM DTT and 10mM GSH.
Soaking conditions as above, minus DTT, plus 2mM Chlorambucil dissolved in ethanol., pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.90 Å R-free 0.222 |
| 3CSJ Human glutathione s-transferase p1-1 in complex with chlorambucil Deposited 2008-04-09 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–210（209 aa）
Chain B
2–210（209 aa）
|
Not recorded | CL CHLORIDE ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 CBL CHLORAMBUCIL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；277 K；Resevior solution contained: 25% (w/v) ammonium sulfate, 60mM DTT, 100mM MES and 10% (v/v) glycerol. Solid chlorambucil was streaked into the drop., pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.233 |
| 3DD3 Crystal Structure of the Glutathione Transferase Pi enzyme in complex with the bifunctional inhibitor, Etharapta Deposited 2008-06-05 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 RUC (eta6-benzene)ruthenium × 1 GSH Glutathione × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；100mM MES buffer pH 5.5 or 6.0, 22%(w/v) PEG 8000, 20mM CaCl2, 10mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.25 Å R-free 0.260 |
| 3DGQ Crystal structure of the glutathione transferase PI enzyme in complex with the bifunctional inhibitor, etharapta Deposited 2008-06-15 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Not recorded | CL CHLORIDE ION × 2 EAA ETHACRYNIC ACID × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 CA CALCIUM ION × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；100mM MES BUFFER PH 5.5, 22%(W/V) PEG 8000, 20mM CACL2, 10mM DTT, Small amounts of solid compound were streaked through the drops containing preformed crystals. These were soaked for 48hours., VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.60 Å R-free 0.227 |
| 3GSS HUMAN GLUTATHIONE S-TRANSFERASE P1-1 IN COMPLEX WITH ETHACRYNIC ACID-GLUTATHIONE CONJUGATE Deposited 1996-10-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Not recorded | SO4 SULFATE ION × 1 GSH Glutathione × 2 EAA ETHACRYNIC ACID × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.2；pH 5.2
|
Resolution 1.90 Å R-free 0.233 |
| 3GUS Crystal strcture of human Pi class glutathione S-transferase GSTP1-1 in complex with 6-(7-Nitro-2,1,3-benzoxadiazol-4-ylthio)hexanol (NBDHEX) Deposited 2009-03-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–210（209 aa）
Chain B
2–210（209 aa）
|
Not recorded | GSH Glutathione × 2 N11 6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)sulfanyl]hexan-1-ol × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；1.8M Ammonium sulfate, 100mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.53 Å R-free 0.195 |
| 3HJM Crystal structure of human Glutathione Transferase Pi Y108V mutant Deposited 2009-05-22 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–210（209 aa）
Chain B
2–210（209 aa）
|
Mutation：Y108V Mutation：Y108V | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 CA CALCIUM ION × 2 CO3 CARBONATE ION × 3 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；298 K；270mM Calcium Acetate, 20% PEG8000, 100mM MES, pH5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.10 Å R-free 0.230 |
| 3HJM Crystal structure of human Glutathione Transferase Pi Y108V mutant Deposited 2009-05-22 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
2–210（209 aa）
Chain D
2–210（209 aa）
|
Mutation：Y108V Mutation：Y108V | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 CA CALCIUM ION × 3 CO3 CARBONATE ION × 2 CL CHLORIDE ION × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；298 K；270mM Calcium Acetate, 20% PEG8000, 100mM MES, pH5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.10 Å R-free 0.230 |
| 3HJO Crystal Structure of Glutathione Transferase Pi Y108V Mutant in Complex with the Glutathione Conjugate of Ethacrynic Acid Deposited 2009-05-22 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–210（209 aa）
Chain B
2–210（209 aa）
|
Mutation：Y108V Mutation：Y108V | GSH Glutathione × 2 EAA ETHACRYNIC ACID × 2 CA CALCIUM ION × 6 CO3 CARBONATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；298 K；133mM Calcium Acetate, 18% PEG8000, 100mM MES, pH5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.95 Å R-free 0.210 |
| 3HKR Crystal Structure of Glutathione Transferase Pi Y108V Mutant Deposited 2009-05-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–210（209 aa）
Chain B
2–210（209 aa）
|
Mutation：Y108V Mutation：Y108V | CA CALCIUM ION × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 CO3 CARBONATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；267mM Calcium Acetate, 20% PEG8000, 100mM MES, pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.80 Å R-free 0.210 |
| 3IE3 Structural basis for the binding of the anti-cancer compound 6-(7-Nitro-2,1,3-benzoxadiazol-4-ylthio)hexanol (NBDHEX) to human glutathione S-transferases Deposited 2009-07-22 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–210（209 aa）
Chain B
2–210（209 aa）
|
Mutation：I104A Mutation：I104A | GSH Glutathione × 2 N11 6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)sulfanyl]hexan-1-ol × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；1.8M AMMONIUM SULFATE, 100MM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.209 |
| 3KM6 Crystal Structure of the Human GST Pi C47S/Y108V Double Mutant in Complex with the Ethacrynic Acid-Glutathione Conjugate Deposited 2009-11-10 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–210（209 aa）
Chain B
2–210（209 aa）
|
Mutation：C47S, Y108V Mutation：C47S, Y108V | EAA ETHACRYNIC ACID × 2 GSH Glutathione × 2 CA CALCIUM ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；4mg/ml, 267mM calcium acetate, 100mM MES pH 6.0, 20% (w/v) PEG 8000 , VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.10 Å R-free 0.206 |
| 3KMN Crystal Structure of the Human Apo GST Pi C47S/Y108V Double Mutant Deposited 2009-11-11 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–210（209 aa）
Chain B
2–210（209 aa）
|
Mutation：C47S, Y108V Mutation：C47S, Y108V | CA CALCIUM ION × 6 CO3 CARBONATE ION × 2 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；7.5mg/ml C47S/Y108V GST, 22% (w/v) PEG 8000, 100mM MES pH 6.0, 350mM Ca acetate, 10mM DTT, vapor diffusion, hanging drop, temperature 298K
|
Resolution 1.80 Å R-free 0.210 |
| 3KMO Crystal Structure of the Human GST Pi C47S/Y108V Double Mutant in Complex with the Ethacrynic Acid-Glutathione Conjugate (Grown in the Absence of the Reducing Agent DTT) Deposited 2009-11-11 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–210（209 aa）
Chain B
2–210（209 aa）
|
Mutation：C47S, Y108V Mutation：C47S, Y108V | GSH Glutathione × 2 CA CALCIUM ION × 6 EAA ETHACRYNIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；4mg/ml, 267mM calcium acetate, 100mM MES pH 6.0, 20% (w/v) PEG 8000 , VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.60 Å R-free 0.254 |
| 3N9J Structure of human Glutathione Transferase Pi class in complex with Ethacraplatin Deposited 2010-05-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Not recorded | PT PLATINUM (II) ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 EAA ETHACRYNIC ACID × 2 CA CALCIUM ION × 3 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；100mM MES, pH 5.5, pH 6.0, 28% (w/v) PEG 8000, 20mM CaCl2 10mM DTT, 3mM EACPT, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.85 Å R-free 0.218 |
| 3PGT CRYSTAL STRUCTURE OF HGSTP1-1[I104] COMPLEXED WITH THE GSH CONJUGATE OF (+)-ANTI-BPDE Deposited 1999-03-22 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Not recorded | SO4 SULFATE ION × 7 GBX 2-AMINO-4-[1-(CARBOXYMETHYL-CARBAMOYL)-2-(9-HYDROXY-7,8-DIOXO-7,8,9,10-TETRAHYDRO-BENZO[DEF]CHRYSEN-10-YLSULFANYL)-ETHYLCARBAMOYL]-BUTYRIC ACID × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.14 Å R-free 0.159 |
| 4GSS HUMAN GLUTATHIONE S-TRANSFERASE P1-1 Y108F MUTANT Deposited 1997-01-20 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Chain B
1–209（209 aa）
|
Mutation：Y108F Mutation：Y108F | GTX S-HEXYLGLUTATHIONE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6；pH 6.0
|
Resolution 2.50 Å R-free 0.262 |
| 4PGT CRYSTAL STRUCTURE OF HGSTP1-1[V104] COMPLEXED WITH THE GSH CONJUGATE OF (+)-ANTI-BPDE Deposited 1999-03-22 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Mutation：VAL 104 VARIANT Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：VAL 104 VARIANT Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 2 GBX 2-AMINO-4-[1-(CARBOXYMETHYL-CARBAMOYL)-2-(9-HYDROXY-7,8-DIOXO-7,8,9,10-TETRAHYDRO-BENZO[DEF]CHRYSEN-10-YLSULFANYL)-ETHYLCARBAMOYL]-BUTYRIC ACID × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6；CRYSTALS WERE GROWN IN HANGING DROPS WHICH INITIALLY CONSISTED OF 4.3 MG/ML
PROTEIN, 0.55 MM GSH CONJUGATE OF (+)-ANTI-BPDE, 0.8 M AMMONIUM SULFATE IN 50
MM MES BUFFER (PH 6.0). THE DROPS WERE EQUILIBRATED AT 293 K AGAINST WELL
SOLUTION CONTAINING 1.6 M AMMONIUM SULFATE IN 0.1 M MES BUFFER (PH 6.0).
|
Resolution 2.10 Å R-free 0.179 |
| 5DAK Crystal Structure of human Glutathione Transferase Pi complexed with a metalloid in the absence of Glutathione Deposited 2015-08-20 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 ARS ARSENIC × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；100MM MES, PH 6.0, 28% (W/V) PEG 8000, 20MM CACL2 10MM DTT. Soaked in 2mM PAO dissolved in DMSO
|
Resolution 2.11 Å R-free 0.204 |
| 5DAL Crystal Structure of human Glutathione Transferase Pi complexed with a metalloid in the presence of Glutathione Deposited 2015-08-20 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 5AU Di-glutathione-PhenylArsine × 2 GSH Glutathione × 2 PA0 Phenylarsine oxide × 2 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；100MM MES, PH 6.0, 28% (W/V) PEG 8000, 20MM CACL2, 10MM DTT, 10MM GSH. Soaked in 2MM PAO dissolved in DMSO
|
Resolution 1.50 Å R-free 0.212 |
| 5DCG Crystal Structure of WT Apo Human Glutathione Transferase Pi Deposited 2015-08-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 PO4 PHOSPHATE ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；100MM MES, PH 6.0, 28% (W/V) PEG 8000, 20MM CACL2, 10MM DTT
|
Resolution 2.01 Å R-free 0.190 |
| 5DDL Crystal Structure of WT Human Glutathione Transferase Pi soaked with a metalloid then back-soaked with glutathione Deposited 2015-08-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 5AU Di-glutathione-PhenylArsine × 2 GSH Glutathione × 2 SO4 SULFATE ION × 1 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；100MM MES, PH 6.0, 28% (W/V) PEG 8000, 20MM CACL2, 10MM DTT. Soaked in 2mM PAO dissolved in DMSO, Then back soaked in 10mM GSH after 2 days
|
Resolution 1.98 Å R-free 0.178 |
| 5DJL Structure of WT Human Glutathione Transferase in complex with cisplatin in the presence of glutathione. Deposited 2015-09-02 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 PT PLATINUM (II) ION × 3 GSH Glutathione × 2 CA CALCIUM ION × 2 CO3 CARBONATE ION × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；100MM MES, PH 5.5, PH 6.0, 28% (W/V) PEG 8000, 20MM CACL2 10MM DTT, 10MM GSH. Soaked in 0.1MM Cisplatin for 24hrs.
|
Resolution 1.80 Å R-free 0.177 |
| 5DJM Structure of WT Human Glutathione Transferase in complex with cisplatin in the absence of glutathione. Deposited 2015-09-02 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 PT PLATINUM (II) ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；100MM MES, PH 5.5, PH 6.0, 28% (W/V) PEG 8000, 20MM CACL2 10MM DTT. Soaked in 0.1MM Cisplatin for 24hrs
|
Resolution 1.90 Å R-free 0.240 |
| 5GSS HUMAN GLUTATHIONE S-TRANSFERASE P1-1, COMPLEX WITH GLUTATHIONE Deposited 1997-08-11 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Fragment：TWO INTACT MONOMERS
Chain B
1–209（209 aa）
Fragment：TWO INTACT MONOMERS
|
Not recorded | GSH Glutathione × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.8；pH 5.8
|
Resolution 1.95 Å R-free 0.255 |
| 5J41 Glutathione S-transferase bound with hydrolyzed Piperlongumine Deposited 2016-03-31 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–210（209 aa）
Chain B
2–210（209 aa）
|
Not recorded | 3LF 3-(3,4,5-trimethoxyphenyl)propanoic acid × 2 GSH Glutathione × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；1.6M ammonium sulfate, 100mM MES pH 6.5
|
Resolution 1.19 Å R-free 0.186 |
| 5JCW Crystal Structure of hGSTP1-1 with Glutathione Adduct of Phenethyl Isothiocyanate Deposited 2016-04-15 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Not recorded | GVX L-gamma-glutamyl-S-[(2-phenylethyl)carbamothioyl]-L-cysteinylglycine × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ACT ACETATE ION × 2 CA CALCIUM ION × 8 GSH Glutathione × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；PEG 8000, MES, Ca(OAC)2
|
Resolution 1.95 Å R-free 0.193 |
| 5L6X CRYSTAL STRUCTURE OF HGSTP1-1 COMPLEXED WITH FERROCENE-GLUTATHIONE CONJUGATE Deposited 2016-06-01 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Mutation：P2A Mutation：P2A | AZI AZIDE ION × 11 6SG S-[N-(ferrocenylmethyl)carbamoylmethyl]-glutathione × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；100 mM HEPES pH 6, 10 mM DTT, 100 mM CaCl2, 30% PEG 6000, Ligand was soaked o/n prior diffraction
|
Resolution 2.00 Å R-free 0.213 |
| 5X79 Human GST Pi conjugated with novel inhibitor, GS-ESF Deposited 2017-02-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Not recorded | GF5 (2S)-2-azanyl-5-[[(2R)-3-(2-fluorosulfonylethylsulfanyl)-1-(2-hydroxy-2-oxoethylamino)-1-oxidanylidene-propan-2-yl]amino]-5-oxidanylidene-pentanoic acid × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；100 mM MES, pH 6.0, 22% PEG8000, 20 mM calcium chloride, 10 mM DTT
|
Resolution 1.90 Å R-free 0.242 |
| 6AP9 Crystal Structure of hGSTP1-1 with S-nitrosation of Cys101 Deposited 2017-08-17 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | GSH Glutathione × 2 GDS OXIDIZED GLUTATHIONE DISULFIDE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 ACT ACETATE ION × 2 CA CALCIUM ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；PEG 8000, MES, Ca(OAc)2
|
Resolution 1.55 Å R-free 0.181 |
| 6GSS HUMAN GLUTATHIONE S-TRANSFERASE P1-1, COMPLEX WITH GLUTATHIONE Deposited 1997-08-13 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Fragment：TWO INTACT MONOMERS
Chain B
1–209（209 aa）
Fragment：TWO INTACT MONOMERS
|
Not recorded | GSH Glutathione × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.4；pH 5.4
|
Resolution 1.90 Å R-free 0.218 |
| 6LLX Discovery of A Dual Inhibitor of NQO1 and GSTP1 for Treating Malignant Glioblastoma Deposited 2019-12-24 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–210（210 aa）
|
Not recorded | GSH Glutathione × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1M MES PH5.8 ,30% PEG6000 ,10mM DTT
|
Resolution 1.58 Å R-free 0.223 |
| 6LLX Discovery of A Dual Inhibitor of NQO1 and GSTP1 for Treating Malignant Glioblastoma Deposited 2019-12-24 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–210（210 aa）
|
Not recorded | GSH Glutathione × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1M MES PH5.8 ,30% PEG6000 ,10mM DTT
|
Resolution 1.58 Å R-free 0.223 |
| 6Y1E Crystal structure of human glutathione transferase P1-1 (hGSTP1-1) that was co-crystallised in the presence of indanyloxyacetic acid-94 (IAA-94) Deposited 2020-02-12 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Not recorded | GSH Glutathione × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 O7Z 2-[[6,7-bis(chloranyl)-2-cyclopentyl-2-methyl-1-oxidanylidene-3~{H}-inden-5-yl]oxy]ethanoic acid × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；10 mg/ml hGSTP1-1 (50 mM dipotassium phosphate, 1.5 mM EDTA, 10 mM DTT, 10 mM GSH and 0.02 % (w/v) sodium azide, pH 7.0) with 3.5 mM IAA-94 (5% DMSO (v/v)) was crystallized in 100 mM MES and 16 % (w/v) PEG-6000, pH 6.5. The crystals were harvested and then immersed two to three times in 100% (v/v) Parabar 10312 (Paratone)
|
Resolution 1.40 Å R-free 0.251 |
| 6Y1E Crystal structure of human glutathione transferase P1-1 (hGSTP1-1) that was co-crystallised in the presence of indanyloxyacetic acid-94 (IAA-94) Deposited 2020-02-12 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1–210（210 aa）
Chain D
1–210（210 aa）
|
Not recorded | GSH Glutathione × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 O7Z 2-[[6,7-bis(chloranyl)-2-cyclopentyl-2-methyl-1-oxidanylidene-3~{H}-inden-5-yl]oxy]ethanoic acid × 1 PGE TRIETHYLENE GLYCOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；10 mg/ml hGSTP1-1 (50 mM dipotassium phosphate, 1.5 mM EDTA, 10 mM DTT, 10 mM GSH and 0.02 % (w/v) sodium azide, pH 7.0) with 3.5 mM IAA-94 (5% DMSO (v/v)) was crystallized in 100 mM MES and 16 % (w/v) PEG-6000, pH 6.5. The crystals were harvested and then immersed two to three times in 100% (v/v) Parabar 10312 (Paratone)
|
Resolution 1.40 Å R-free 0.251 |
| 7BIA Crystal structure of human GSTP1 bound to iberin Deposited 2021-01-12 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–210（210 aa）
Chain B
1–210（210 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 GSH Glutathione × 2 TVQ 1-isothiocyanato-3-methylsulfinyl-propane × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1M MES pH 6.0, 25% PEG8000, 0.02M CaCl2, 5mM DTT
|
Resolution 1.73 Å R-free 0.214 |
| 7GSS Human glutathione S-transferase P1-1, complex with glutathione Deposited 1997-08-13 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Fragment：TWO INTACT MONOMERS
Chain B
1–209（209 aa）
Fragment：TWO INTACT MONOMERS
|
Not recorded | GSH Glutathione × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.2；pH 6.2
|
Resolution 2.20 Å R-free 0.259 |
| 7XBA Glutathione S-transferase bound with a covalent inhibitor Deposited 2022-03-21 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–210（209 aa）
Chain B
2–210（209 aa）
|
Not recorded | GSH Glutathione × 1 HJ6 3-[3-[[2-[5-[(3,5-dimethyl-4-nitro-pyrazol-1-yl)methyl]furan-2-yl]-5-(methylcarbamoyl)benzimidazol-1-yl]methyl]azetidin-1-yl]sulfonylbenzenesulfonic acid × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；289 K；Magnesium acetate tetrahydrate, Sodium cacodylate trihydrate pH 6.5, Polyethylene glycol 8000
|
Resolution 2.83 Å R-free 0.248 |
| 8GSS Human glutathione S-transferase P1-1, complex with glutathione Deposited 1997-08-14 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Fragment：THREE INTACT MONOMERS
Chain B
1–209（209 aa）
Fragment：THREE INTACT MONOMERS
|
Not recorded | SO4 SULFATE ION × 1 GSH Glutathione × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.4；pH 5.4
|
Resolution 1.90 Å R-free 0.229 |
| 8GSS Human glutathione S-transferase P1-1, complex with glutathione Deposited 1997-08-14 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1–209（209 aa）
Fragment：THREE INTACT MONOMERS
|
Not recorded | SO4 SULFATE ION × 2 GSH Glutathione × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.4；pH 5.4
|
Resolution 1.90 Å R-free 0.229 |
| 9GSS HUMAN GLUTATHIONE S-TRANSFERASE P1-1, COMPLEX WITH S-HEXYL GLUTATHIONE Deposited 1997-08-14 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–209（209 aa）
Fragment：TWO INTACT MONOMERS
Chain B
1–209（209 aa）
Fragment：TWO INTACT MONOMERS
|
Not recorded | SO4 SULFATE ION × 1 GTX S-HEXYLGLUTATHIONE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6；pH 6.0
|
Resolution 1.97 Å R-free 0.232 |