当前蛋白身份：P0A546
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1DF7 DIHYDROFOLATE REDUCTASE OF MYCOBACTERIUM TUBERCULOSIS COMPLEXED WITH NADPH AND METHOTREXATE 提交 1999-11-17 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–159（159 aa）
|
未记录 | SO4 SULFATE ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 MTX METHOTREXATE × 1 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 4.5；277 K；AMMONIUM SULFATE, SODIUM ACETATE, GLYCEROL, NADPH, METHOTREXATE, POTASSIUM
PHOSPHATE, DTT, POTASSIUM CHLORIDE, pH 4.5, VAPOR DIFFUSION, temperature 277K
|
分辨率 1.70 Å R-free 0.246 |
| 1DG5 DIHYDROFOLATE REDUCTASE OF MYCOBACTERIUM TUBERCULOSIS COMPLEXED WITH NADPH AND TRIMETHOPRIM 提交 1999-11-23 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–159（159 aa）
|
未记录 | NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 TOP TRIMETHOPRIM × 1 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.5；277 K；AMMONIUM SULFATE, SODIUM ACETATE, GLYCEROL, NADPH, TRIMETHOPRIM, POTASSIUM
PHOSPHATE, DTT, POTASSIUM CHLORIDE, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
分辨率 2.00 Å R-free 0.243 |
| 1DG7 DIHYDROFOLATE REDUCTASE OF MYCOBACTERIUM TUBERCULOSIS COMPLEXED WITH NADPH AND 4-BROMO WR99210 提交 1999-11-23 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–159（159 aa）
|
未记录 | WRB 1-[3-(4-BROMO-PHENOXY)-PROPOXY]-6,6-DIMETHYL-1.6-DIHYDRO-[1,3,5]TRIAZINE-2,4-DIAMINE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.5；277 K；AMMONIUM SULFATE, SODIUM ACETATE, GLYCEROL, NADPH, BROMO-WR99210, POTASSIUM
PHOSPHATE, DTT, POTASSIUM CHLORIDE, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
分辨率 1.80 Å R-free 0.246 |
| 1DG8 DIHYDROFOLATE REDUCTASE OF MYCOBACTERIUM TUBERCULOSIS COMPLEXED WITH NADPH 提交 1999-11-23 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–159（159 aa）
|
未记录 | NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 GOL GLYCEROL × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.5；277 K；AMMONIUM SULFATE, SODIUM ACETATE, GLYCEROL, NADPH, POTASSIUM PHOSPHATE, DTT,
POTASSIUM CHLORIDE, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
分辨率 2.00 Å R-free 0.244 |
| 2CIG Dihydrofolate reductase from Mycobacterium tuberculosis inhibited by the acyclic 4R isomer of INH-NADP a derivative of the prodrug isoniazid. 提交 2006-03-20 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–159（159 aa）
|
未记录 | 1DG (4R)-ISONICOTINIC-ACETYL-NICOTINAMIDE-ADENINE DINUCLEOTIDE × 1 GOL GLYCEROL × 4 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 4.5；1.6 M AMMONIUM SULFATE, 100 MM SODIUM ACETATE, PH 4.6, 10 % GLYCEROL
|
分辨率 1.90 Å R-free 0.212 |
| 4KL9 Crystal structure of dihydrofolate reductase from Mycobacterium tuberculosis in the space group C2 提交 2013-05-07 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–159（159 aa）
|
未记录 | NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 P33 3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；Mes, PEGMME 2000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.39 Å R-free 0.202 |
| 4KLX Crystal structure of dihydrofolate reductase from Mycobacterium tuberculosis in an open conformation. 提交 2013-05-07 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–159（159 aa）
|
未记录 | ATR 2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE × 1 ETE 2-{2-[2-2-(METHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；8% PEG 4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 1.23 Å R-free 0.244 |
| 4KLX Crystal structure of dihydrofolate reductase from Mycobacterium tuberculosis in an open conformation. 提交 2013-05-07 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–159（159 aa）
|
未记录 | ATR 2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；8% PEG 4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 1.23 Å R-free 0.244 |
| 4KM0 Crystal structure of dihydrofolate reductase from Mycobacterium tuberculosis in complex with pyrimethamine 提交 2013-05-07 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–159（159 aa）
|
未记录 | CP6 5-(4-CHLORO-PHENYL)-6-ETHYL-PYRIMIDINE-2,4-DIAMINE × 1 ATR 2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；8% PEG 4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 1.30 Å R-free 0.210 |
| 4KM0 Crystal structure of dihydrofolate reductase from Mycobacterium tuberculosis in complex with pyrimethamine 提交 2013-05-07 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–159（159 aa）
|
未记录 | CP6 5-(4-CHLORO-PHENYL)-6-ETHYL-PYRIMIDINE-2,4-DIAMINE × 1 ATR 2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；8% PEG 4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 1.30 Å R-free 0.210 |
| 4KM2 Crystal structure of Dihydrofolate reductase from Mycobacterium tuberculosis in an open conformation in complex with trimethoprim 提交 2013-05-07 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–159（159 aa）
|
未记录 | ATR 2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE × 1 TOP TRIMETHOPRIM × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；0.05M KCl, 0.01M MgCl2, 15% PEG 6000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 1.40 Å R-free 0.201 |
| 4KM2 Crystal structure of Dihydrofolate reductase from Mycobacterium tuberculosis in an open conformation in complex with trimethoprim 提交 2013-05-07 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–159（159 aa）
|
未记录 | ATR 2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE × 1 TOP TRIMETHOPRIM × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；0.05M KCl, 0.01M MgCl2, 15% PEG 6000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 1.40 Å R-free 0.201 |
| 4KNE Crystal structure of dihydrofolate reductase from Mycobacterium tuberculosis in complex with cycloguanil 提交 2013-05-09 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–159（159 aa）
|
未记录 | 1CY 1-(4-chlorophenyl)-6,6-dimethyl-1,6-dihydro-1,3,5-triazine-2,4-diamine × 1 ATR 2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；8% PEG 4000, pH 7.0, VAPOR DIFFUSION, SITTING DROP
|
分辨率 2.00 Å R-free 0.270 |
| 4KNE Crystal structure of dihydrofolate reductase from Mycobacterium tuberculosis in complex with cycloguanil 提交 2013-05-09 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–159（159 aa）
|
未记录 | 1CY 1-(4-chlorophenyl)-6,6-dimethyl-1,6-dihydro-1,3,5-triazine-2,4-diamine × 1 ATR 2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；8% PEG 4000, pH 7.0, VAPOR DIFFUSION, SITTING DROP
|
分辨率 2.00 Å R-free 0.270 |
| 4M2X Mycobacterium tuberculosis dihydrofolate reductase complexed with trimetrexate (TMQ) 提交 2013-08-05 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–159（159 aa）
|
未记录 | NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 TMQ TRIMETREXATE × 1 ACT ACETATE ION × 6 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.6；289 K；15% PEG 6000, 100 mM sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 2.26 Å R-free 0.261 |
| 4M2X Mycobacterium tuberculosis dihydrofolate reductase complexed with trimetrexate (TMQ) 提交 2013-08-05 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 C
1–159（159 aa）
|
未记录 | NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 TMQ TRIMETREXATE × 1 ACT ACETATE ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.6；289 K；15% PEG 6000, 100 mM sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 2.26 Å R-free 0.261 |
| 4M2X Mycobacterium tuberculosis dihydrofolate reductase complexed with trimetrexate (TMQ) 提交 2013-08-05 | Assembly 3 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 E
1–159（159 aa）
|
未记录 | NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 TMQ TRIMETREXATE × 1 ACT ACETATE ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.6；289 K；15% PEG 6000, 100 mM sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 2.26 Å R-free 0.261 |
| 4M2X Mycobacterium tuberculosis dihydrofolate reductase complexed with trimetrexate (TMQ) 提交 2013-08-05 | Assembly 4 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 G
1–159（159 aa）
|
未记录 | NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 TMQ TRIMETREXATE × 1 ACT ACETATE ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.6；289 K；15% PEG 6000, 100 mM sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 2.26 Å R-free 0.261 |