当前蛋白身份:P0A910 重新检索
本组结构的主要差异维度
构建体不同 突变/修饰不同 组装状态不同 配体/离子不同 实验方法不同 实验环境不同 结构质量指标不同

差异标签只比较当前检索结果;所有PDB和assembly原始记录仍分别保留。

相关结构差异明细

一行代表一个 PDB 条目中的一个 biological assembly;同一蛋白的多个单体会分别列出。

PDB 条目 Assembly / 聚集状态 构建体 突变与修饰 配体、离子与非聚合物 实验方法 实验环境 结构质量
1BXW OUTER MEMBRANE PROTEIN A (OMPA) TRANSMEMBRANE DOMAIN 提交 1998-10-03 Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 A 21–192(172 aa) 片段:TRANSMEMBRANE DOMAIN
突变:F23L, Q34K, K107Y C8E (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE × 1 X-RAY DIFFRACTION
X-ray结晶条件 pH 5;10 % PEG-8000 10 % MPD 0.05 M POTASSIUM PHOSPHATE PH 5.0
分辨率 2.50 Å R-free 0.235
1G90 NMR Solution Structure of Outer Membrane Protein A Transmembrane Domain: 10 conformers 提交 2000-11-21 Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 A 22–197(176 aa) 片段:TRANSMEMBRANE DOMAIN, RESIDUES 1-176
突变:W15F, W57F, W102F, W103F. 未记录非水小分子 SOLUTION NMR
NMR测量条件 pH 6.3;323 K;离子强度(mmCIF原始值)0.050;压力 ambient
NMR样品组成 1mM OmpA(0-176) U-15N,13C,2H; 600 mM D38-DPC; 10 mM phosphate buffer; pH 6.3; 50 mM NaCl | 95% H2O/5% D2O
分辨率未提供
2GE4 High-resolution solution structure of outer membrane protein A transmembrane domain 提交 2006-03-17 Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 A 22–197(176 aa) 片段:transmembrane domain
突变:W15F, W57F, W102F, W143F 未记录非水小分子 SOLUTION NMR
NMR测量条件 pH 6.3;223 K;离子强度(mmCIF原始值)50mM NaCl;压力 ambient
NMR样品组成 1mM OmpA U-2H,13C,15N; 500mM DPC; 10mM phosphate buffer; pH 6.3; 50mM NaCl | 90% H2O/10% D2O
分辨率未提供
2JMM NMR solution structure of a minimal transmembrane beta-barrel platform protein 提交 2006-11-20 Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 A 43–197(155 aa)
未记录 未记录非水小分子 SOLUTION NMR
NMR测量条件 pH 6;303 K;离子强度(mmCIF原始值)20;压力 ambient
NMR样品组成 1 mM [U-100% 13C; U-100% 15N; 80% 2H] BBP+EF, 95% H2O/5% D2O | 95% H2O/5% D2O
分辨率未提供
3NB3 The host outer membrane proteins OmpA and OmpC are packed at specific sites in the Shigella phage Sf6 virion as structural components 提交 2010-06-02 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 240 PDB 声明:240-MERIC(240) 与蛋白数一致
链 A 1–346(346 aa) 片段:outer membrane protein A
链 B 1–346(346 aa) 片段:outer membrane protein A
链 C 1–346(346 aa) 片段:outer membrane protein A
未记录 未记录非水小分子 ELECTRON MICROSCOPY
cryo-EM缓冲液 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2;pH 7.4;10 mM Tris-HCl, pH 7.4, 10 mM MgCl2
cryo-EM玻璃化条件 冷冻剂 ETHANE;Vitrification was carried out with a standard cryoEM method. The sample of 4 microlitres was applied to a holey EM grid, blotted for 3-4 seconds with a Whatman #2 filter paper, and plunged into ethane slush incubated in liquid nitrogen. The grid was stored in liquid nitrogen.
分辨率 19.00 Å
3NB3 The host outer membrane proteins OmpA and OmpC are packed at specific sites in the Shigella phage Sf6 virion as structural components 提交 2010-06-02 Assembly 2 蛋白异源复合物 异源复合物;蛋白 × 4 PDB 声明:tetrameric(4) 与蛋白数一致
链 A 1–346(346 aa) 片段:outer membrane protein A
链 B 1–346(346 aa) 片段:outer membrane protein A
链 C 1–346(346 aa) 片段:outer membrane protein A
未记录 未记录非水小分子 ELECTRON MICROSCOPY
cryo-EM缓冲液 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2;pH 7.4;10 mM Tris-HCl, pH 7.4, 10 mM MgCl2
cryo-EM玻璃化条件 冷冻剂 ETHANE;Vitrification was carried out with a standard cryoEM method. The sample of 4 microlitres was applied to a holey EM grid, blotted for 3-4 seconds with a Whatman #2 filter paper, and plunged into ethane slush incubated in liquid nitrogen. The grid was stored in liquid nitrogen.
分辨率 19.00 Å
3NB3 The host outer membrane proteins OmpA and OmpC are packed at specific sites in the Shigella phage Sf6 virion as structural components 提交 2010-06-02 Assembly 3 蛋白异源复合物 异源复合物;蛋白 × 20 PDB 声明:eicosameric(20) 与蛋白数一致
链 A 1–346(346 aa) 片段:outer membrane protein A
链 B 1–346(346 aa) 片段:outer membrane protein A
链 C 1–346(346 aa) 片段:outer membrane protein A
未记录 未记录非水小分子 ELECTRON MICROSCOPY
cryo-EM缓冲液 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2;pH 7.4;10 mM Tris-HCl, pH 7.4, 10 mM MgCl2
cryo-EM玻璃化条件 冷冻剂 ETHANE;Vitrification was carried out with a standard cryoEM method. The sample of 4 microlitres was applied to a holey EM grid, blotted for 3-4 seconds with a Whatman #2 filter paper, and plunged into ethane slush incubated in liquid nitrogen. The grid was stored in liquid nitrogen.
分辨率 19.00 Å
3NB3 The host outer membrane proteins OmpA and OmpC are packed at specific sites in the Shigella phage Sf6 virion as structural components 提交 2010-06-02 Assembly 4 蛋白异源复合物 异源复合物;蛋白 × 24 PDB 声明:24-meric(24) 与蛋白数一致
链 A 1–346(346 aa) 片段:outer membrane protein A
链 B 1–346(346 aa) 片段:outer membrane protein A
链 C 1–346(346 aa) 片段:outer membrane protein A
未记录 未记录非水小分子 ELECTRON MICROSCOPY
cryo-EM缓冲液 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2;pH 7.4;10 mM Tris-HCl, pH 7.4, 10 mM MgCl2
cryo-EM玻璃化条件 冷冻剂 ETHANE;Vitrification was carried out with a standard cryoEM method. The sample of 4 microlitres was applied to a holey EM grid, blotted for 3-4 seconds with a Whatman #2 filter paper, and plunged into ethane slush incubated in liquid nitrogen. The grid was stored in liquid nitrogen.
分辨率 19.00 Å
3NB3 The host outer membrane proteins OmpA and OmpC are packed at specific sites in the Shigella phage Sf6 virion as structural components 提交 2010-06-02 Assembly 5 蛋白异源复合物 异源复合物;蛋白 × 4 PDB 声明:tetrameric(4) 与蛋白数一致
链 A 1–346(346 aa) 片段:outer membrane protein A
链 B 1–346(346 aa) 片段:outer membrane protein A
链 C 1–346(346 aa) 片段:outer membrane protein A
未记录 未记录非水小分子 ELECTRON MICROSCOPY
cryo-EM缓冲液 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2;pH 7.4;10 mM Tris-HCl, pH 7.4, 10 mM MgCl2
cryo-EM玻璃化条件 冷冻剂 ETHANE;Vitrification was carried out with a standard cryoEM method. The sample of 4 microlitres was applied to a holey EM grid, blotted for 3-4 seconds with a Whatman #2 filter paper, and plunged into ethane slush incubated in liquid nitrogen. The grid was stored in liquid nitrogen.
分辨率 19.00 Å
5M2Q Structure of cobinamide-bound BtuF mutant W66F, the periplasmic vitamin B12 binding protein in E.coli 提交 2016-10-13 Assembly 1 信息不足 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 A 1–22(22 aa)
突变:W66F CBY COB(II)INAMIDE × 1 CYN CYANIDE ION × 1 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP;293.15 K;PEG3350 HEPES pH 7 Tryptone
分辨率 1.70 Å R-free 0.249
5M2Q Structure of cobinamide-bound BtuF mutant W66F, the periplasmic vitamin B12 binding protein in E.coli 提交 2016-10-13 Assembly 2 信息不足 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 B 1–22(22 aa)
突变:W66F CBY COB(II)INAMIDE × 1 CYN CYANIDE ION × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP;293.15 K;PEG3350 HEPES pH 7 Tryptone
分辨率 1.70 Å R-free 0.249
6LYR Structure of the BAM complex 提交 2020-02-15 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 6 PDB 声明:hexameric(6) 与蛋白数一致
链 P 180–188(9 aa)
未记录 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;289 K;27-30% PEG 400, 100mM NaCl
分辨率 3.28 Å R-free 0.280
9FZC Structure of OmpA-short in complex with nanobody Nb01 提交 2024-07-05 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 A 22–191(170 aa)
未记录 OCT N-OCTANE × 6 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP;pH 5.5;293 K;0.1 M sodium acetate pH 5.5, 0.2 M calcium acetate, 25 % PEG MME 2K
分辨率 2.27 Å R-free 0.234
9FZC Structure of OmpA-short in complex with nanobody Nb01 提交 2024-07-05 Assembly 2 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 B 22–191(170 aa)
未记录 OCT N-OCTANE × 11 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP;pH 5.5;293 K;0.1 M sodium acetate pH 5.5, 0.2 M calcium acetate, 25 % PEG MME 2K
分辨率 2.27 Å R-free 0.234