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Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：P0AE67 New Search
31PDB entries
49Biological assembly
50chain/assembly records
Main Difference Dimensions in This Set
Different construct Different mutation/modification Different assembly state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
1E6K Two-component signal transduction system D12A mutant of CheY Deposited 2000-08-18 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 2–129（128 aa）
Mutation：D12A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；pH 7.00
Resolution 2.00 Å R-free 0.260
1E6L Two-component signal transduction system D13A mutant of CheY Deposited 2000-08-18 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 3–129（127 aa）
Mutation：D13A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；pH 7.00
Resolution 1.90 Å R-free 0.270
1E6M TWO-COMPONENT SIGNAL TRANSDUCTION SYSTEM D57A MUTANT OF CHEY Deposited 2000-08-18 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 2–129（128 aa）
Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；pH 7.00
Resolution 1.70 Å R-free 0.260
1JBE 1.08 A Structure of apo-Chey reveals meta-active conformation Deposited 2001-06-04 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 2–129（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 3 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICRODIALYSIS；pH 8.3；293 K；microdialysis, 2.2M Ammonium sulfate, 50mM Tris, pH 8.3, MICRODIALYSIS, temperature 293K
Resolution 1.08 Å R-free 0.152
2B1J Crystal Structure of Unphosphorylated CheY Bound to the N-Terminus of FliM Deposited 2005-09-15 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；PEG 6000, magnesium chloride, MES buffer, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.40 Å R-free 0.240
2B1J Crystal Structure of Unphosphorylated CheY Bound to the N-Terminus of FliM Deposited 2005-09-15 Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain B 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；PEG 6000, magnesium chloride, MES buffer, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.40 Å R-free 0.240
2ID7 1.75 A Structure of T87I Phosphono-CheY Deposited 2006-09-14 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–128（128 aa）
Mutation：D57(CyQ) T87I Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；28% PEG 3200, 0.2M ammonium chloride, 0.1M sodium acetate, pH 4.60, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 1.75 Å R-free 0.202
2ID9 1.85 A Structure of T87I/Y106W Phosphono-CheY Deposited 2006-09-14 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–128（128 aa）
Mutation：D57(CyQ) T87I Y106W Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.84；298 K；20% PEG 8000, 0.2 M ammonium acetate, 0.1 M MES-NaOH, pH 5.84, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 1.75 Å R-free 0.202
2IDM 2.00 A Structure of T87I/Y106W Phosphono-CheY Deposited 2006-09-15 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–128（128 aa）
Mutation：D57(CyQ) T87I Y106W Non-standard monomer：Yes (specific site not provided by mmCIF) ACT ACETATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.88；298 K；20% PEG 8000, 0.2M ammonium acetate, 0.1 M MES-NaOH, pH 5.88, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.00 Å R-free 0.328
2LP4 Solution structure of P1-CheY/P2 complex in bacterial chemotaxis Deposited 2012-01-31 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain Y 2–129（128 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 0.05；Pressure ambient
NMR sample composition 200 mM [U-15N; U-2H] CheY, 3000 mM [U-2H] CheA P1, 250 mM [U-2H] CheA P2, 92% H2O/8% D2O | 92% H2O/8% D2O
Resolution not provided
3F7N Crystal Structure of CheY triple mutant F14E, N59M, E89L complexed with BeF3- and Mn2+ Deposited 2008-11-09 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 2–129（128 aa）
Mutation：F14E, N59M, E89L MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 GOL GLYCEROL × 2 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；Tris buffer, Ammonium Sulfate, Glycerol, BeCl2, NaF, MnCl2, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.00 Å R-free 0.219
3F7N Crystal Structure of CheY triple mutant F14E, N59M, E89L complexed with BeF3- and Mn2+ Deposited 2008-11-09 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 2–129（128 aa）
Mutation：F14E, N59M, E89L MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；Tris buffer, Ammonium Sulfate, Glycerol, BeCl2, NaF, MnCl2, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.00 Å R-free 0.219
3FFT Crystal Structure of CheY double mutant F14E, E89R complexed with BeF3- and Mn2+ Deposited 2008-12-04 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 2–129（128 aa）
Mutation：F14E, E89R MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 GOL GLYCEROL × 2 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；Tris buffer, Ammonium Sulfate, Glycerol, BeCl2, NaF, MnCl2, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.21 Å R-free 0.203
3FFT Crystal Structure of CheY double mutant F14E, E89R complexed with BeF3- and Mn2+ Deposited 2008-12-04 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 2–129（128 aa）
Mutation：F14E, E89R MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 GOL GLYCEROL × 2 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；Tris buffer, Ammonium Sulfate, Glycerol, BeCl2, NaF, MnCl2, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.21 Å R-free 0.203
3FFW Crystal Structure of CheY triple mutant F14Q, N59K, E89Y complexed with BeF3- and Mn2+ Deposited 2008-12-04 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 2–129（128 aa）
Mutation：F14Q, N59K, E89Y MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 GOL GLYCEROL × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；Tris buffer, Ammonium Sulfate, Glycerol, BeCl2, NaF, MnCl2, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.00 Å R-free 0.206
3FFW Crystal Structure of CheY triple mutant F14Q, N59K, E89Y complexed with BeF3- and Mn2+ Deposited 2008-12-04 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 2–129（128 aa）
Mutation：F14Q, N59K, E89Y MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 GOL GLYCEROL × 2 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；Tris buffer, Ammonium Sulfate, Glycerol, BeCl2, NaF, MnCl2, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.00 Å R-free 0.206
3FFX Crystal Structure of CheY triple mutant F14E, N59R, E89H complexed with BeF3- and Mn2+ Deposited 2008-12-04 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 2–129（128 aa）
Mutation：F14E, N59R, E89H MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 SO4 SULFATE ION × 2 GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；Tris buffer, Ammonium Sulfate, Glycerol, BeCl2, NaF, MnCl2, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.01 Å R-free 0.210
3FFX Crystal Structure of CheY triple mutant F14E, N59R, E89H complexed with BeF3- and Mn2+ Deposited 2008-12-04 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 2–129（128 aa）
Mutation：F14E, N59R, E89H MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 SO4 SULFATE ION × 1 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；Tris buffer, Ammonium Sulfate, Glycerol, BeCl2, NaF, MnCl2, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.01 Å R-free 0.210
3FGZ Crystal Structure of CheY triple mutant F14E, N59M, E89R complexed with BeF3- and Mn2+ Deposited 2008-12-08 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 2–129（128 aa）
Mutation：F14E, N59M, E89R MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；Tris buffer, Ammonium Sulfate, Glycerol, BeCl2, NaF, MnCl2, pH 8.4, vapor diffusion, hanging drop, temperature 298K
Resolution 2.00 Å R-free 0.220
3FGZ Crystal Structure of CheY triple mutant F14E, N59M, E89R complexed with BeF3- and Mn2+ Deposited 2008-12-08 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 2–129（128 aa）
Mutation：F14E, N59M, E89R MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 GOL GLYCEROL × 1 NH4 AMMONIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；Tris buffer, Ammonium Sulfate, Glycerol, BeCl2, NaF, MnCl2, pH 8.4, vapor diffusion, hanging drop, temperature 298K
Resolution 2.00 Å R-free 0.220
3MYY Structure of E. Coli CheY mutant A113P bound to Beryllium fluoride Deposited 2010-05-11 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 2–129（128 aa） Fragment：CheY
Chain B 2–129（128 aa） Fragment：CheY
Mutation：A113P Mutation：A113P BEF BERYLLIUM TRIFLUORIDE ION × 4 MN MANGANESE (II) ION × 6 GOL GLYCEROL × 7 SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；Ammonium Sulfate 1.8-2.4 M, Glycerol 0-12.5% (v/v), Tris 100 mM, pH 7.0-8.5, MnCl2 20mM, BeCl2 1mM, NaF 10mM, , VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.10 Å R-free 0.202
3OLV Structural and functional effects of substitution at position T+1 in CheY: CheYA88V-BeF3-Mg complex Deposited 2010-08-26 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–129（129 aa）
Mutation：A88V MG MAGNESIUM ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 4 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；Ammonium Sulfate 1.7 M Glycerol 5% (v/v) Tris 100 mM pH 8.0 MnCl2 20mM BeCl2 1mM NaF 10mM, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.70 Å R-free 0.209
3OLV Structural and functional effects of substitution at position T+1 in CheY: CheYA88V-BeF3-Mg complex Deposited 2010-08-26 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 1–129（129 aa）
Mutation：A88V MG MAGNESIUM ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 2 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；Ammonium Sulfate 1.7 M Glycerol 5% (v/v) Tris 100 mM pH 8.0 MnCl2 20mM BeCl2 1mM NaF 10mM, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.70 Å R-free 0.209
3OLW Structural and functional effects of substitution at position T+1 in CheY: CheYA88T-BeF3-Mn complex Deposited 2010-08-26 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–129（129 aa）
Mutation：A88T BEF BERYLLIUM TRIFLUORIDE ION × 1 MN MANGANESE (II) ION × 2 GOL GLYCEROL × 4 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Ammonium Sulfate 2.4 M Glycerol 5.0% (v/v) Tris 100 mM pH 7.5 MnCl2 20mM BeCl2 1mM NaF 10mM 10.1 mg/mL CheY A88M 2-19-10, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.30 Å R-free 0.222
3OLW Structural and functional effects of substitution at position T+1 in CheY: CheYA88T-BeF3-Mn complex Deposited 2010-08-26 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 1–129（129 aa）
Mutation：A88T BEF BERYLLIUM TRIFLUORIDE ION × 1 MN MANGANESE (II) ION × 2 GOL GLYCEROL × 4 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Ammonium Sulfate 2.4 M Glycerol 5.0% (v/v) Tris 100 mM pH 7.5 MnCl2 20mM BeCl2 1mM NaF 10mM 10.1 mg/mL CheY A88M 2-19-10, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.30 Å R-free 0.222
3OLX Structural and functional effects of substitution at position T+1 in CheY: CheYA88S-BeF3-Mn complex Deposited 2010-08-26 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–129（129 aa）
Mutation：A88S BEF BERYLLIUM TRIFLUORIDE ION × 2 MN MANGANESE (II) ION × 2 GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；Ammonium Sulfate 2.4 M Glycerol 5% (v/v) Tris 100 mM pH 7.5-8.5 MnCl2 20mM BeCl2 1mM NaF 10mM 7.2 mg/mL CheY A88S 2-15-10 , VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.10 Å R-free 0.230
3OLX Structural and functional effects of substitution at position T+1 in CheY: CheYA88S-BeF3-Mn complex Deposited 2010-08-26 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 1–129（129 aa）
Mutation：A88S BEF BERYLLIUM TRIFLUORIDE ION × 1 MN MANGANESE (II) ION × 3 GOL GLYCEROL × 1 MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；Ammonium Sulfate 2.4 M Glycerol 5% (v/v) Tris 100 mM pH 7.5-8.5 MnCl2 20mM BeCl2 1mM NaF 10mM 7.2 mg/mL CheY A88S 2-15-10 , VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.10 Å R-free 0.230
3OLY Structural and functional effects of substitution at position T+1 in CheY: CheYA88M-BeF3-Mn complex Deposited 2010-08-26 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–129（129 aa）
Mutation：A88M BEF BERYLLIUM TRIFLUORIDE ION × 1 MN MANGANESE (II) ION × 3 GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；Ammonium Sulfate 2.4 M Glycerol 2.5% (v/v) Tris 100 mM pH 8.0 MnCl2 20mM BeCl2 1mM NaF 10mM 4.8 mg/mL CheY A88M 1-10-10, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.05 Å R-free 0.212
3OLY Structural and functional effects of substitution at position T+1 in CheY: CheYA88M-BeF3-Mn complex Deposited 2010-08-26 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 1–129（129 aa）
Mutation：A88M BEF BERYLLIUM TRIFLUORIDE ION × 1 MN MANGANESE (II) ION × 3 GOL GLYCEROL × 2 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；Ammonium Sulfate 2.4 M Glycerol 2.5% (v/v) Tris 100 mM pH 8.0 MnCl2 20mM BeCl2 1mM NaF 10mM 4.8 mg/mL CheY A88M 1-10-10, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.05 Å R-free 0.212
3OO0 Structure of apo CheY A113P Deposited 2010-08-30 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–129（129 aa） Fragment：CheY
Mutation：A113P GOL GLYCEROL × 4 MN MANGANESE (II) ION × 1 NH4 AMMONIUM ION × 1 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.25；298 K；Ammonium Sulfate 2.2 M Tris 50 mM pH 8.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.55 Å R-free 0.199
3OO0 Structure of apo CheY A113P Deposited 2010-08-30 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 1–129（129 aa） Fragment：CheY
Mutation：A113P GOL GLYCEROL × 3 MN MANGANESE (II) ION × 1 NH4 AMMONIUM ION × 1 SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.25；298 K；Ammonium Sulfate 2.2 M Tris 50 mM pH 8.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.55 Å R-free 0.199
3OO1 Structure of E. Coli CheY mutant A113P in the absence of Sulfate Deposited 2010-08-30 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–129（129 aa）
Mutation：A113P MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；28% PEG 8000 100 mM Na Cacodylate pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.70 Å R-free 0.199
3OO1 Structure of E. Coli CheY mutant A113P in the absence of Sulfate Deposited 2010-08-30 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 1–129（129 aa）
Mutation：A113P MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；28% PEG 8000 100 mM Na Cacodylate pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.70 Å R-free 0.199
3RVJ Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89Q Deposited 2011-05-06 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–129（129 aa）
Mutation：N59D, E89Q MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 SO4 SULFATE ION × 2 GOL GLYCEROL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.25；298 K；Ammonium Sulfate 2.4M, Tris 100mM pH 8.25, Glycerol 5% (v/v), MnCl2 20mM, BeCl2 1mM, NaF 10mM, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.10 Å R-free 0.201
3RVJ Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89Q Deposited 2011-05-06 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 1–129（129 aa）
Mutation：N59D, E89Q MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 SO4 SULFATE ION × 2 GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.25；298 K；Ammonium Sulfate 2.4M, Tris 100mM pH 8.25, Glycerol 5% (v/v), MnCl2 20mM, BeCl2 1mM, NaF 10mM, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.10 Å R-free 0.201
3RVK Structure of the CheY-Mn2+ Complex with substitutions at 59 and 89: N59D E89Q Deposited 2011-05-06 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–129（129 aa）
Mutation：N59D, E89Q MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；PEG 8000 31% (w/v), Na Cacodylate 100mM, pH 6.0, Calcium Acetate 140mM, Glycerol 10% (v/v), 8.8 mg/mL CheY, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.16 Å R-free 0.159
3RVL Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89R Deposited 2011-05-06 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–129（129 aa）
Mutation：N59D, E89R BEF BERYLLIUM TRIFLUORIDE ION × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.25；298 K；Ammonium Sulfate 2.5M, Tris 100mM pH 8.25, Glycerol 5% (v/v), MnCl2 20mM, BeCl2 1mM, NaF 10mM, 4.25mg/mL CheY, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.55 Å R-free 0.182
3RVL Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89R Deposited 2011-05-06 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 1–129（129 aa）
Mutation：N59D, E89R BEF BERYLLIUM TRIFLUORIDE ION × 1 MN MANGANESE (II) ION × 2 SO4 SULFATE ION × 1 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.25；298 K；Ammonium Sulfate 2.5M, Tris 100mM pH 8.25, Glycerol 5% (v/v), MnCl2 20mM, BeCl2 1mM, NaF 10mM, 4.25mg/mL CheY, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.55 Å R-free 0.182
3RVM Structure of the CheY-Mn2+ Complex with substitutions at 59 and 89: N59D and E89R Deposited 2011-05-06 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–129（129 aa）
Mutation：N59D, E89R MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.25；298 K；PEG 8000 26%, Na Cacodylate 100mM pH 6.0, Calcium Acetate 120mM, Glycerol 10% (v/v), 4.25 mg/mL CheY, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.45 Å R-free 0.166
3RVN Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89Y Deposited 2011-05-06 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–129（129 aa）
Mutation：N59D, E89Y MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 SO4 SULFATE ION × 2 GOL GLYCEROL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.25；298 K；Ammonium Sulfate 2.0M Tris 100mM pH 8.25 Glycerol 5% (v/v) MnCl2 20mM BeCl2 1mM NaF 10mM CheY 8.9mg/mL, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.25 Å R-free 0.225
3RVN Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89Y Deposited 2011-05-06 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 1–129（129 aa）
Mutation：N59D, E89Y MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 SO4 SULFATE ION × 2 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.25；298 K；Ammonium Sulfate 2.0M Tris 100mM pH 8.25 Glycerol 5% (v/v) MnCl2 20mM BeCl2 1mM NaF 10mM CheY 8.9mg/mL, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.25 Å R-free 0.225
3RVO Structure of CheY-Mn2+ Complex with substitutions at 59 and 89: N59D E89Y Deposited 2011-05-06 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–129（129 aa）
Mutation：N59D, E89Y MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；PEG 8000 30%, Na Cacodylate 100mM pH 6.0, Calcium Acetate 120mM, Glycerol 10% (v/v), 8.9 mg/mL CheY, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.55 Å R-free 0.185
3RVP Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89K Deposited 2011-05-06 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–129（129 aa）
Mutation：N59D, E89K MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 SO4 SULFATE ION × 3 GOL GLYCEROL × 6 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Ammonium Sulfate 2.6M, Tris 100mM pH 7.5, Glycerol 5% (v/v), MnCl2 20mM, BeCl2 1mM, NaF 10mM, 4.6mg/mL, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.40 Å R-free 0.215
3RVP Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89K Deposited 2011-05-06 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 1–129（129 aa）
Mutation：N59D, E89K MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 SO4 SULFATE ION × 3 GOL GLYCEROL × 5 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；Ammonium Sulfate 2.6M, Tris 100mM pH 7.5, Glycerol 5% (v/v), MnCl2 20mM, BeCl2 1mM, NaF 10mM, 4.6mg/mL, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.40 Å R-free 0.215
3RVQ Structure of the CheY-Mn2+ Complex with substitutions at 59 and 89: N59D E89K Deposited 2011-05-06 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–129（129 aa）
Mutation：N59D, E89K MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；PEG 8000 28% (w/v), Na Cacodylate 100 mM pH 6.0, Calcium Acetate 120mM, Glycerol 10% (v/v), 4.6 mg/mL CheY, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.15 Å R-free 0.125
3RVR Structure of the CheYN59D/E89R Molybdate complex Deposited 2011-05-06 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–129（129 aa）
Mutation：N59D, E89R MN MANGANESE (II) ION × 1 MOO MOLYBDATE ION × 1 SO4 SULFATE ION × 4 GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；2.45 M Ammonium Sulfate, 100 mM Tris, pH 7.5, 5% (v/v) Glycerol, 2 mM Ammonium Molybdate, 20mM Manganese chloride, 4.25 mg/mL CheY, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.10 Å R-free 0.188
3RVR Structure of the CheYN59D/E89R Molybdate complex Deposited 2011-05-06 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 1–129（129 aa）
Mutation：N59D, E89R MN MANGANESE (II) ION × 1 MOO MOLYBDATE ION × 1 SO4 SULFATE ION × 7 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；2.45 M Ammonium Sulfate, 100 mM Tris, pH 7.5, 5% (v/v) Glycerol, 2 mM Ammonium Molybdate, 20mM Manganese chloride, 4.25 mg/mL CheY, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.10 Å R-free 0.188
3RVS Structure of the CheYN59D/E89R Tungstate complex Deposited 2011-05-06 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–129（129 aa）
Mutation：N59D, E89R MN MANGANESE (II) ION × 1 WO4 TUNGSTATE(VI)ION × 1 SO4 SULFATE ION × 3 GOL GLYCEROL × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；2.6 M Ammonium Sulfate, 100 mM Tris, pH 7.5, 5% (v/v) glycerol, 10 mM Sodium Tungstate, 20 mM Manganese chloride, 4.25 mg/mL CheY, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.10 Å R-free 0.184
3RVS Structure of the CheYN59D/E89R Tungstate complex Deposited 2011-05-06 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 1–129（129 aa）
Mutation：N59D, E89R MN MANGANESE (II) ION × 1 WO4 TUNGSTATE(VI)ION × 1 SO4 SULFATE ION × 5 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；2.6 M Ammonium Sulfate, 100 mM Tris, pH 7.5, 5% (v/v) glycerol, 10 mM Sodium Tungstate, 20 mM Manganese chloride, 4.25 mg/mL CheY, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.10 Å R-free 0.184

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.