Current Protein Identity：P0AFG0
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2JVV Solution Structure of E. coli NusG carboxyterminal domain Deposited 2007-09-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–181（181 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.4；298 K；Pressure ambient
NMR sample composition
0.45 mM [U-95% 13C; U-95% 15N] NusG, 10 mM potassium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2K06 Solution structure of the aminoterminal domain of E. coli NusG Deposited 2008-01-25 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–123（123 aa）
Fragment：N-terminal domain, residues 1-123
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.4；298 K；Pressure ambient
NMR sample composition
0.4 mM [U-98% 13C; U-98% 15N] NusG, 10 mM potassium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2KVQ Solution structure of NusE:NusG-CTD complex Deposited 2010-03-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
123–181（59 aa）
Fragment：UNP residues 123-181, KOW domain
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.5；310 K；Ionic strength (raw mmCIF value) 50
NMR sample composition
300 uM [U-100% 13C; U-100% 15N] 15N, 13C -NusG-CTD-1, 300 uM NusE-2, 300 uM NusB-3, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
300 uM [U-100% 13C; U-100% 15N; U-80% 2H] 15N, 13C, 2H-NusE-4, 300 uM NusB-5, 300 uM NusG-CTD-6, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.3 mM NusG-CTD-7, 0.3 mM [U-100% 13C; U-100% 15N] 15N, 13C NusE-8, 0.3 mM NusB-9, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 5MS0 pseudo-atomic model of the RNA polymerase lambda-based antitermination complex solved by cryo-EM Deposited 2016-12-29 | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: tetradecameric（14） Consistent with all polymers |
Chain F
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.80 Å |
| 6XAV CryoEM Structure of E. coli Rho-dependent Transcription Pre-termination Complex bound with NusG Deposited 2020-06-04 | Assembly 1 Other combination Heteromer；Protein × 13 PDB declaration: hexadecameric（16） Consistent with all polymers |
Chain L
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.70 Å |
| 6XE0 Cryo-EM structure of NusG-CTD bound to 70S ribosome (30S: NusG-CTD fragment) Deposited 2020-06-11 | Assembly 1 Protein–RNA Heteromer；Protein × 21 PDB declaration: 22-meric（22） Consistent with all polymers |
Chain V
1–181（181 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.80 Å |
| 7PY0 CryoEM structure of E.coli RNA polymerase elongation complex bound to NusG (NusG-EC in more-swiveled conformation) Deposited 2021-10-08 | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: nonameric（9） Consistent with all polymers |
Chain G
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 7PY1 CryoEM structure of E.coli RNA polymerase elongation complex bound to NusG (the consensus NusG-EC) Deposited 2021-10-08 | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: nonameric（9） Consistent with all polymers |
Chain G
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 7PY5 CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA and NusG (the consensus NusA-NusG-EC) Deposited 2021-10-09 | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: decameric（10） Consistent with all polymers |
Chain G
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 7PY6 CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA and NusG (NusA and NusG elongation complex in less-swiveled conformation) Deposited 2021-10-09 | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: decameric（10） Consistent with all polymers |
Chain G
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 7PY7 CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA and NusG (NusA and NusG elongation complex in more-swiveled conformation) Deposited 2021-10-09 | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: decameric（10） Consistent with all polymers |
Chain G
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 7PY8 CryoEM structure of E.coli RNA polymerase elongation complex bound to NusG (NusG-EC in less-swiveled conformation) Deposited 2021-10-09 | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: nonameric（9） Consistent with all polymers |
Chain G
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8Y5K E.coli transcription translation coupling complex in TTC-A state 2 containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain NG
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.90 Å |
| 8Y5L E.coli transcription translation coupling complex in TTC-B state 1 (subclass1) containing mRNA with 30-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain NG
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 8Y5M E.coli transcription translation coupling complex in TTC-B state 2 containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain NG
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 8Y5N E.coli transcription translation coupling complex in TTC-A state 3 containing mRNA with 21-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain NG
1–181（181 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 8Y5O E.coli transcription translation coupling complex in TTC-B state 3 (subclass1) containing mRNA with 30-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain NG
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 8Y5P E.coli transcription translation coupling complex in TTC-B state 4 (subclass 1) containing mRNA with 24-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-01-31 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain NG
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.50 Å |
| 8Y5Q E.coli transcription translation coupling complex in TTC-B state 4 (subclass 2) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and GDPCP Deposited 2024-01-31 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain NG
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 8Y5R E.coli Transcription translation coupling complex in TTC-B state 5 (subclass 1) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and fusidic acid Deposited 2024-01-31 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain NG
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 8Y5S E.coli Transcription translation coupling complex in TTC-B state 5 (subclass 2) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and GDPCP Deposited 2024-01-31 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain NG
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 8Y5T E.coli Transcription translation coupling complex in TTC-B state 5 (subclass 3) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and fusidic acid Deposited 2024-01-31 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain NG
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å |
| 8YDE E.coli transcription translation coupling complex in TTC-B state 1 (subclass 3) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain NG
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.00 Å |
| 8YDF E.coli transcription translation coupling complex in TTC-B state 1 (subclass 2) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain NG
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 8YDG E.coli transcription translation coupling complex in TTC-B state 3 (subclass2) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain NG
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.10 Å |
| 8YDH E.coli transcription translation coupling complex in TTC-P state 1 (subclass1) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain NG
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.55 Å |
| 8YDI E.coli transcription translation coupling complex in TTC-P state 1 (subclass 2) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain NG
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 8YDJ E.coli transcription translation coupling complex in TTC-P containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin Deposited 2024-02-20 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain NG
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.10 Å |
| 9GUR 30S mRNA delivery complex TEC resolved (TEC only) Deposited 2024-09-20 | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: nonameric（9） Consistent with all polymers |
Chain Z
6–118（113 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 9GUW 30S-TEC (TEC in expressome position) Inactive state 2 Deposited 2024-09-20 | Assembly 1 Other combination Heteromer；Protein × 26 PDB declaration: 30-meric（30） Consistent with all polymers |
Chain Z
1–181（181 aa）
|
Not recorded | MG MAGNESIUM ION × 118 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9GUX 30S-TEC (TEC in expressome position) Inactive state 1 Deposited 2024-09-20 | Assembly 1 Other combination Heteromer；Protein × 27 PDB declaration: 31-meric（31） Consistent with all polymers |
Chain Z
1–180（180 aa）
|
Not recorded | MG MAGNESIUM ION × 132 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |