Current Protein Identity:P10957
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1A04 THE STRUCTURE OF THE NITRATE/NITRITE RESPONSE REGULATOR PROTEIN NARL IN THE MONOCLINIC C2 CRYSTAL FORM Deposited 1997-12-08 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
2–216(215 aa)
Fragment:RESIDUES 2 - 216 OF THE WILD TYPE NARL
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.6;277 K;THE PROTEIN SOLUTION CONTAINING 23.3 MG/ML OF PURIFIED NARL, 20 MM TRIS.HCL (PH=7.6), 0.5 MM MGCL2, AND 10% GLYCEROL WAS MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION CONTAINING 0.1 M TRIS.HCL (PH=8.5), 0.2 M SODIUM ACETATE, AND 30% POLYETHYLENE GLYCOL (PEG) 4000. SITTING DROPS CONTAINING 20 ML OF MIXTURE WERE EQUILIBRATED BY VAPOR DIFFUSION AT 4 DEGREES AGAINST 20 ML OF THE RESERVOIR SOLUTION. CRYSTALS BEGAN TO APPEAR AS CLUSTERS OF PLATES AFTER 11-15 DAYS., vapor diffusion - sitting drop, temperature 277K
|
Resolution 2.20 Å R-free 0.267 |
| 1A04 THE STRUCTURE OF THE NITRATE/NITRITE RESPONSE REGULATOR PROTEIN NARL IN THE MONOCLINIC C2 CRYSTAL FORM Deposited 1997-12-08 | Assembly 2 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain B
2–216(215 aa)
Fragment:RESIDUES 2 - 216 OF THE WILD TYPE NARL
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.6;277 K;THE PROTEIN SOLUTION CONTAINING 23.3 MG/ML OF PURIFIED NARL, 20 MM TRIS.HCL (PH=7.6), 0.5 MM MGCL2, AND 10% GLYCEROL WAS MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION CONTAINING 0.1 M TRIS.HCL (PH=8.5), 0.2 M SODIUM ACETATE, AND 30% POLYETHYLENE GLYCOL (PEG) 4000. SITTING DROPS CONTAINING 20 ML OF MIXTURE WERE EQUILIBRATED BY VAPOR DIFFUSION AT 4 DEGREES AGAINST 20 ML OF THE RESERVOIR SOLUTION. CRYSTALS BEGAN TO APPEAR AS CLUSTERS OF PLATES AFTER 11-15 DAYS., vapor diffusion - sitting drop, temperature 277K
|
Resolution 2.20 Å R-free 0.267 |
| 1JE8 Two-Component response regulator NarL/DNA Complex: DNA Bending Found in a High Affinity Site Deposited 2001-06-15 | Assembly 1 Protein–DNA Homooligomer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
147–216(70 aa)
Fragment:DNA Binding Domain (147-216)
Chain B
147–216(70 aa)
Fragment:DNA Binding Domain (147-216)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7.5;298 K;ammonium sulfate, TRIS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 2.12 Å R-free 0.273 |
| 1JE8 Two-Component response regulator NarL/DNA Complex: DNA Bending Found in a High Affinity Site Deposited 2001-06-15 | Assembly 2 Protein–DNA Homooligomer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain E
147–216(70 aa)
Fragment:DNA Binding Domain (147-216)
Chain F
147–216(70 aa)
Fragment:DNA Binding Domain (147-216)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7.5;298 K;ammonium sulfate, TRIS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 2.12 Å R-free 0.273 |
| 1RNL THE NITRATE/NITRITE RESPONSE REGULATOR PROTEIN NARL FROM NARL Deposited 1996-04-17 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
2–216(215 aa)
Fragment:RESIDUES 2 - 216 OF THE WILD TYPE NARL
|
Not recorded | PT PLATINUM (II) ION × 6 GOL GLYCEROL × 3 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.40 Å R-free 0.251 |
| 1RNL THE NITRATE/NITRITE RESPONSE REGULATOR PROTEIN NARL FROM NARL Deposited 1996-04-17 | Assembly 2 Protein homooligomer Homooligomer;Protein × 4 PDB declaration: tetrameric(4) Consistent with protein count |
Chain A
2–216(215 aa)
Fragment:RESIDUES 2 - 216 OF THE WILD TYPE NARL
|
Not recorded | PT PLATINUM (II) ION × 24 GOL GLYCEROL × 12 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.40 Å R-free 0.251 |
| 1RNL THE NITRATE/NITRITE RESPONSE REGULATOR PROTEIN NARL FROM NARL Deposited 1996-04-17 | Assembly 3 Protein homooligomer Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
2–216(215 aa)
Fragment:RESIDUES 2 - 216 OF THE WILD TYPE NARL
|
Not recorded | PT PLATINUM (II) ION × 12 GOL GLYCEROL × 6 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.40 Å R-free 0.251 |