Current Protein Identity：P15291
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2AE7 Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide Deposited 2005-07-21 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T | MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.246 |
| 2AE7 Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide Deposited 2005-07-21 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T | MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 6 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.246 |
| 2AE7 Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide Deposited 2005-07-21 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T | MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.246 |
| 2AE7 Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide Deposited 2005-07-21 | Assembly 4 Other combination Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
Chain B
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
Chain C
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T Mutation：M340H, C338T, R337T Mutation：M340H, C338T, R337T | MN MANGANESE (II) ION × 3 SO4 SULFATE ION × 14 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 3 GOL GLYCEROL × 7 DIO 1,4-DIETHYLENE DIOXIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.246 |
| 2AEC Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with GlcNAc-beta1,2-Man-alpha1,6-Man-beta-OR Deposited 2005-07-22 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T | MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.239 |
| 2AEC Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with GlcNAc-beta1,2-Man-alpha1,6-Man-beta-OR Deposited 2005-07-22 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T | MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 6 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.239 |
| 2AEC Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with GlcNAc-beta1,2-Man-alpha1,6-Man-beta-OR Deposited 2005-07-22 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T | MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.239 |
| 2AES Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with GlcNAc-beta1,2-Man-alpha1,3-Man-beta-OR Deposited 2005-07-23 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T | MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.232 |
| 2AES Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with GlcNAc-beta1,2-Man-alpha1,3-Man-beta-OR Deposited 2005-07-23 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T | MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 6 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.232 |
| 2AES Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with GlcNAc-beta1,2-Man-alpha1,3-Man-beta-OR Deposited 2005-07-23 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T | MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 3 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.232 |
| 2AGD Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I(M340H-B4Gal-T1) in Complex with GlcNAc-beta1,4-Man-alpha1,3-Man-beta-OR Deposited 2005-07-26 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T | MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.228 |
| 2AGD Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I(M340H-B4Gal-T1) in Complex with GlcNAc-beta1,4-Man-alpha1,3-Man-beta-OR Deposited 2005-07-26 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T | MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 7 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.228 |
| 2AGD Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I(M340H-B4Gal-T1) in Complex with GlcNAc-beta1,4-Man-alpha1,3-Man-beta-OR Deposited 2005-07-26 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T | MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 5 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.228 |
| 2AH9 Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with Chitotriose Deposited 2005-07-27 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T | UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 2 SO4 SULFATE ION × 4 DIO 1,4-DIETHYLENE DIOXIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.224 |
| 2AH9 Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with Chitotriose Deposited 2005-07-27 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T | UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 4 SO4 SULFATE ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.224 |
| 2AH9 Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with Chitotriose Deposited 2005-07-27 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T | UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 1 SO4 SULFATE ION × 5 DIO 1,4-DIETHYLENE DIOXIDE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.224 |
| 2AH9 Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with Chitotriose Deposited 2005-07-27 | Assembly 4 Other combination Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
Chain B
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
Chain C
125–397（273 aa）
Fragment：Catalytic domain, Residues 126-398
|
Mutation：M340H, C338T, R337T Mutation：M340H, C338T, R337T Mutation：M340H, C338T, R337T | UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 3 MN MANGANESE (II) ION × 3 GOL GLYCEROL × 7 SO4 SULFATE ION × 16 DIO 1,4-DIETHYLENE DIOXIDE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.224 |
| 2FY7 Crystal structure of the catalytic domain of the human beta1,4-galactosyltransferase mutant M339H in apo form Deposited 2006-02-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
125–397（273 aa）
Fragment：catalytic domain, residues 125-397
|
Mutation：R337T, C338T, M340H | PGE TRIETHYLENE GLYCOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.6；298 K；50 mM sodium citrate buffer, 6% PEG 4000, pH 5.6, VAPOR DIFFUSION, temperature 298K
|
Resolution 1.70 Å R-free 0.222 |
| 2FYA Crystal structure of the catalytic domain of the human beta1, 4-galactosyltransferase mutant M339H complex with manganese Deposited 2006-02-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
125–397（273 aa）
Fragment：catalytic domain, residues 125-397
|
Mutation：R337T, C338T, M340H | MN MANGANESE (II) ION × 1 PGE TRIETHYLENE GLYCOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.6；298 K；10 mg/ml protein solution, 5 mM manganese chloride, 50 mM sodium citrate buffer, 6% PEG 4000, pH 5.6, VAPOR DIFFUSION, temperature 298K
|
Resolution 1.90 Å R-free 0.226 |
| 2FYB Crystal structure of the catalytic domain of the human beta1,4-galactosyltransferase mutant M339H in complex with Mn and UDP-galactose in open conformation Deposited 2006-02-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
125–397（273 aa）
Fragment：catalytic domain, residues 125-397
|
Mutation：R337T, C338T, M340H | MN MANGANESE (II) ION × 1 UDP URIDINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.6；298 K；10 mg/ml protein solution, 5mM manganese chloride, 5mM UDP-galactose, 50 mM sodium citrate, 6% PEG 4000, pH 5.6, VAPOR DIFFUSION, temperature 298K
|
Resolution 1.90 Å R-free 0.216 |
| 3EE5 Crystal structure of human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in complex with GLCNAC-Beta1,3-Gal-Beta-Naphthalenemethanol Deposited 2008-09-04 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：Catalytic domain of Beta-1,4-Galactosyltransferase
|
Mutation：d126, M340H, C338T, R337T | UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 2NA naphthalen-2-ylmethanol × 1 SO4 SULFATE ION × 5 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；pH 6.5；298 K；MES, AMMONIUM SULFATE, DIOXANE, pH 6.5, EVAPORATION, temperature 298K
|
Resolution 2.20 Å R-free 0.240 |
| 3EE5 Crystal structure of human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in complex with GLCNAC-Beta1,3-Gal-Beta-Naphthalenemethanol Deposited 2008-09-04 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
126–398（273 aa）
Fragment：Catalytic domain of Beta-1,4-Galactosyltransferase
|
Mutation：d126, M340H, C338T, R337T | UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 2NA naphthalen-2-ylmethanol × 1 SO4 SULFATE ION × 7 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；pH 6.5；298 K；MES, AMMONIUM SULFATE, DIOXANE, pH 6.5, EVAPORATION, temperature 298K
|
Resolution 2.20 Å R-free 0.240 |
| 3EE5 Crystal structure of human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in complex with GLCNAC-Beta1,3-Gal-Beta-Naphthalenemethanol Deposited 2008-09-04 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
126–398（273 aa）
Fragment：Catalytic domain of Beta-1,4-Galactosyltransferase
|
Mutation：d126, M340H, C338T, R337T | UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 2NA naphthalen-2-ylmethanol × 1 SO4 SULFATE ION × 2 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；pH 6.5；298 K；MES, AMMONIUM SULFATE, DIOXANE, pH 6.5, EVAPORATION, temperature 298K
|
Resolution 2.20 Å R-free 0.240 |
| 4EE3 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with pentasaccharide Deposited 2012-03-28 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：Catalytic domain
|
Mutation：R337T, C338T, M340H | UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 8 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.30 Å R-free 0.240 |
| 4EE3 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with pentasaccharide Deposited 2012-03-28 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
126–398（273 aa）
Fragment：Catalytic domain
|
Mutation：R337T, C338T, M340H | UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 6 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.30 Å R-free 0.240 |
| 4EE3 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with pentasaccharide Deposited 2012-03-28 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
126–398（273 aa）
Fragment：Catalytic domain
|
Mutation：R337T, C338T, M340H | UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.30 Å R-free 0.240 |
| 4EE3 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with pentasaccharide Deposited 2012-03-28 | Assembly 4 Other combination Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：Catalytic domain
Chain B
126–398（273 aa）
Fragment：Catalytic domain
Chain C
126–398（273 aa）
Fragment：Catalytic domain
|
Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H | UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 6 MN MANGANESE (II) ION × 6 SO4 SULFATE ION × 36 GOL GLYCEROL × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.30 Å R-free 0.240 |
| 4EE3 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with pentasaccharide Deposited 2012-03-28 | Assembly 5 Other combination Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：Catalytic domain
Chain B
126–398（273 aa）
Fragment：Catalytic domain
Chain C
126–398（273 aa）
Fragment：Catalytic domain
|
Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H | UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 3 MN MANGANESE (II) ION × 3 SO4 SULFATE ION × 18 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.30 Å R-free 0.240 |
| 4EE4 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with tetrasaccharide from Lacto-N-neohexose Deposited 2012-03-28 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：Catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 7 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.95 Å R-free 0.232 |
| 4EE4 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with tetrasaccharide from Lacto-N-neohexose Deposited 2012-03-28 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
126–398（273 aa）
Fragment：Catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 11 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.95 Å R-free 0.232 |
| 4EE4 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with tetrasaccharide from Lacto-N-neohexose Deposited 2012-03-28 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
126–398（273 aa）
Fragment：Catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.95 Å R-free 0.232 |
| 4EE4 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with tetrasaccharide from Lacto-N-neohexose Deposited 2012-03-28 | Assembly 4 Other combination Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：Catalytic domain
Chain B
126–398（273 aa）
Fragment：Catalytic domain
Chain C
126–398（273 aa）
Fragment：Catalytic domain
|
Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 6 MN MANGANESE (II) ION × 6 SO4 SULFATE ION × 44 GOL GLYCEROL × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.95 Å R-free 0.232 |
| 4EE4 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with tetrasaccharide from Lacto-N-neohexose Deposited 2012-03-28 | Assembly 5 Other combination Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：Catalytic domain
Chain B
126–398（273 aa）
Fragment：Catalytic domain
Chain C
126–398（273 aa）
Fragment：Catalytic domain
|
Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 3 MN MANGANESE (II) ION × 3 SO4 SULFATE ION × 22 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.95 Å R-free 0.232 |
| 4EE5 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with trisaccharide from Lacto-N-neotetraose Deposited 2012-03-28 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 1 SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.250 |
| 4EE5 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with trisaccharide from Lacto-N-neotetraose Deposited 2012-03-28 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 2 SO4 SULFATE ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.250 |
| 4EE5 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with trisaccharide from Lacto-N-neotetraose Deposited 2012-03-28 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 1 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.250 |
| 4EE5 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with trisaccharide from Lacto-N-neotetraose Deposited 2012-03-28 | Assembly 4 Other combination Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：catalytic domain
Chain B
126–398（273 aa）
Fragment：catalytic domain
Chain C
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 6 MN MANGANESE (II) ION × 6 GOL GLYCEROL × 8 SO4 SULFATE ION × 22 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.250 |
| 4EE5 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with trisaccharide from Lacto-N-neotetraose Deposited 2012-03-28 | Assembly 5 Other combination Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：catalytic domain
Chain B
126–398（273 aa）
Fragment：catalytic domain
Chain C
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 3 MN MANGANESE (II) ION × 3 GOL GLYCEROL × 4 SO4 SULFATE ION × 11 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.250 |
| 4EEA Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta1,4-Glc-BETA Deposited 2012-03-28 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 1 SO4 SULFATE ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 MM Mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.240 |
| 4EEA Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta1,4-Glc-BETA Deposited 2012-03-28 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 2 SO4 SULFATE ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 MM Mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.240 |
| 4EEA Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta1,4-Glc-BETA Deposited 2012-03-28 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 1 SO4 SULFATE ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 MM Mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.240 |
| 4EEA Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta1,4-Glc-BETA Deposited 2012-03-28 | Assembly 4 Other combination Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：catalytic domain
Chain B
126–398（273 aa）
Fragment：catalytic domain
Chain C
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 6 MN MANGANESE (II) ION × 6 GOL GLYCEROL × 8 SO4 SULFATE ION × 40 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 MM Mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.240 |
| 4EEA Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta1,4-Glc-BETA Deposited 2012-03-28 | Assembly 5 Other combination Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：catalytic domain
Chain B
126–398（273 aa）
Fragment：catalytic domain
Chain C
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 3 MN MANGANESE (II) ION × 3 GOL GLYCEROL × 4 SO4 SULFATE ION × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 MM Mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.240 |
| 4EEG Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta Deposited 2012-03-28 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 1 SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M Ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.244 |
| 4EEG Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta Deposited 2012-03-28 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 2 SO4 SULFATE ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M Ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.244 |
| 4EEG Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta Deposited 2012-03-28 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 1 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M Ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.244 |
| 4EEG Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta Deposited 2012-03-28 | Assembly 4 Other combination Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：catalytic domain
Chain B
126–398（273 aa）
Fragment：catalytic domain
Chain C
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 3 MN MANGANESE (II) ION × 3 GOL GLYCEROL × 4 SO4 SULFATE ION × 10 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M Ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.244 |
| 4EEM Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-MAN-ALPHA-methyl Deposited 2012-03-28 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：Catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.236 |
| 4EEM Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-MAN-ALPHA-methyl Deposited 2012-03-28 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
126–398（273 aa）
Fragment：Catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 7 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.236 |
| 4EEM Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-MAN-ALPHA-methyl Deposited 2012-03-28 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
126–398（273 aa）
Fragment：Catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 3 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.236 |
| 4EEM Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-MAN-ALPHA-methyl Deposited 2012-03-28 | Assembly 4 Other combination Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：Catalytic domain
Chain B
126–398（273 aa）
Fragment：Catalytic domain
Chain C
126–398（273 aa）
Fragment：Catalytic domain
|
Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 3 MN MANGANESE (II) ION × 3 SO4 SULFATE ION × 14 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.236 |
| 4EEO Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-GlcNAc-ALPHA-benzyl Deposited 2012-03-28 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.30 Å R-free 0.255 |
| 4EEO Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-GlcNAc-ALPHA-benzyl Deposited 2012-03-28 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 5 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.30 Å R-free 0.255 |
| 4EEO Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-GlcNAc-ALPHA-benzyl Deposited 2012-03-28 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 2 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.30 Å R-free 0.255 |
| 4EEO Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-GlcNAc-ALPHA-benzyl Deposited 2012-03-28 | Assembly 4 Other combination Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
126–398（273 aa）
Fragment：catalytic domain
Chain B
126–398（273 aa）
Fragment：catalytic domain
Chain C
126–398（273 aa）
Fragment：catalytic domain
|
Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H Mutation：R337T, C338T, M340H | UDP URIDINE-5'-DIPHOSPHATE × 3 MN MANGANESE (II) ION × 3 SO4 SULFATE ION × 11 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.30 Å R-free 0.255 |
| 4L41 Human Lactose synthase: A 2:1 complex between human alpha-lactalbumin and human beta1,4-galactosyltransferase Deposited 2013-06-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
126–398（273 aa）
Fragment：UNP residues 126-398
|
Mutation：R337T, C338T | CA CALCIUM ION × 2 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；100 mM mes buffer pH 7.0, 12% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.237 |
| 6FWT Crystal structure of human wild type beta-1,4-galactosyltransferase-1 (B4GalT1) in apo-open monomeric form Deposited 2018-03-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
122–398（277 aa）
|
Not recorded | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.2 M Potassium Nitrate, 20% (w/v) PEG 3350
|
Resolution 1.84 Å R-free 0.218 |
| 6FWU Crystal structure of human wild type beta-1,4-galactosyltransferase-1 (B4GalT1) in apo-closed dimeric form Deposited 2018-03-07 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
126–398（273 aa）
Chain B
126–398（273 aa）
|
Not recorded | NO3 NITRATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Potassium Nitrate, 20% (w/v) PEG 3350
|
Resolution 2.35 Å R-free 0.256 |