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Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：P18708 New Search
42PDB entries
43Biological assembly
234chain/assembly records
Main Difference Dimensions in This Set
Different construct Different mutation/modification Different assembly state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
1D2N D2 DOMAIN OF N-ETHYLMALEIMIDE-SENSITIVE FUSION PROTEIN Deposited 1998-06-30 Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain A 477–740（264 aa） Fragment：D2
Not recorded MG MAGNESIUM ION × 6 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 6 GOL GLYCEROL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8；293 K；3-8% PEG 3350, 300 MM KCL, 100 MM TRIS-HCL, PH 8.0, 5 MM MGCL2, 5 MM GDCL3, 2 MM DTT, 2 MM AMP-PNP, temperature 293K
Resolution 1.75 Å R-free 0.223
1NSF D2 HEXAMERIZATION DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF) Deposited 1998-06-26 Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain A 478–744（267 aa） Fragment：D2 HEXAMERIZATION DOMAIN
Not recorded MG MAGNESIUM ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.05；pH 6.05
Resolution 1.90 Å R-free 0.244
1QCS N-TERMINAL DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF) Deposited 1999-05-14 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 1–205（205 aa） Fragment：N-TERMINAL DOMAIN OF NSF
Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.7；293 K；Vapor diffusion, hanging drop, 100 mM Tris pH 8.7, 2.0 M ammonium sulfate, 10 mM dithiothreitol (DTT), VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.90 Å R-free 0.242
1QDN AMINO TERMINAL DOMAIN OF THE N-ETHYLMALEIMIDE SENSITIVE FUSION PROTEIN (NSF) Deposited 1999-05-21 Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count
Chain A 1–203（203 aa） Fragment：AMINO TERMINAL DOMAIN
Chain B 1–203（203 aa） Fragment：AMINO TERMINAL DOMAIN
Chain C 1–203（203 aa） Fragment：AMINO TERMINAL DOMAIN
Not recorded SO4 SULFATE ION × 8 BME BETA-MERCAPTOETHANOL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.2；TRIS, AMMONIUM SULPHATE, 2- MERCAPTOETHANOL, pH 8.2
Resolution 2.30 Å R-free 0.260
1QDN AMINO TERMINAL DOMAIN OF THE N-ETHYLMALEIMIDE SENSITIVE FUSION PROTEIN (NSF) Deposited 1999-05-21 Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain A 1–203（203 aa） Fragment：AMINO TERMINAL DOMAIN
Chain B 1–203（203 aa） Fragment：AMINO TERMINAL DOMAIN
Chain C 1–203（203 aa） Fragment：AMINO TERMINAL DOMAIN
Not recorded SO4 SULFATE ION × 16 BME BETA-MERCAPTOETHANOL × 12 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.2；TRIS, AMMONIUM SULPHATE, 2- MERCAPTOETHANOL, pH 8.2
Resolution 2.30 Å R-free 0.260
3J94 Structure of ATP-bound N-ethylmaleimide sensitive factor determined by single particle cryoelectron microscopy Deposited 2014-12-05 Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 11 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM Tris-Cl, 150 mM NaCl, 1 mM EDTA, 1 mM ATP, 1 mM DTT, 0.05% v/v Nonident P-40；pH 8；50 mM Tris-Cl, 150 mM NaCl, 1 mM EDTA, 1 mM ATP, 1 mM DTT, 0.05% v/v Nonident P-40
cryo-EM vitrification conditions Blot for 3.5 seconds before plunging；90 K；Cryogen ETHANE；Blot for 3.5 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).
Resolution 4.20 Å R-free 0.302
3J95 Structure of ADP-bound N-ethylmaleimide sensitive factor determined by single particle cryoelectron microscopy Deposited 2014-12-05 Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 4 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM Tris-Cl, 150 mM NaCl, 1 mM EDTA, 1 mM ATP, 1 mM DTT, 0.05% v/v Nonident P-40；pH 8；50 mM Tris-Cl, 150 mM NaCl, 1 mM EDTA, 1 mM ATP, 1 mM DTT, 0.05% v/v Nonident P-40
cryo-EM vitrification conditions Blot for 3.5 seconds before plunging；90 K；Cryogen ETHANE；Blot for 3.5 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).
Resolution 7.60 Å R-free 0.265
3J96 Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State I) Deposited 2014-12-05 Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric（13） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 50 mM Tris-Cl, 150 mM NaCl, 1 mM AMPPNP, 1 mM EDTA, 1 mM DTT, 0.05% v/v Nonident P-40；pH 8；50 mM Tris-Cl, 150 mM NaCl, 1 mM AMPPNP, 1 mM EDTA, 1 mM DTT, 0.05% v/v Nonident P-40
cryo-EM vitrification conditions Blot for 3.5 seconds before plunging；90 K；Cryogen ETHANE；Blot for 3.5 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).
Resolution 7.60 Å R-free 0.303
3J97 Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State II) Deposited 2014-12-05 Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric（13） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 50 mM Tris-Cl, 150 mM NaCl, 1 mM AMPPNP, 1 mM EDTA, 1 mM DTT, 0.05% v/v Nonident P-40；pH 8；50 mM Tris-Cl, 150 mM NaCl, 1 mM AMPPNP, 1 mM EDTA, 1 mM DTT, 0.05% v/v Nonident P-40
cryo-EM vitrification conditions Blot for 3.5 seconds before plunging；90 K；Cryogen ETHANE；Blot for 3.5 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).
Resolution 7.80 Å R-free 0.311
3J98 Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State IIIa) Deposited 2014-12-05 Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric（13） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 50 mM Tris-Cl, 150 mM NaCl, 1 mM AMPPNP, 1 mM EDTA, 1 mM DTT, 0.05% v/v Nonident P-40；pH 8；50 mM Tris-Cl, 150 mM NaCl, 1 mM AMPPNP, 1 mM EDTA, 1 mM DTT, 0.05% v/v Nonident P-40
cryo-EM vitrification conditions Blot for 3.5 seconds before plunging.；90 K；Cryogen ETHANE；Blot for 3.5 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).
Resolution 8.40 Å R-free 0.328
3J99 Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State IIIb) Deposited 2014-12-05 Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric（13） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 50 mM Tris-Cl, 150 mM NaCl, 1 mM AMPPNP, 1 mM EDTA, 1 mM DTT, 0.05% v/v Nonident P-40；pH 8；50 mM Tris-Cl, 150 mM NaCl, 1 mM AMPPNP, 1 mM EDTA, 1 mM DTT, 0.05% v/v Nonident P-40
cryo-EM vitrification conditions Blot for 3.5 seconds before plunging.；77 K；Cryogen ETHANE；Blot for 3.5 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).
Resolution 8.20 Å R-free 0.304
6IP2 NSF-D1D2 part in the whole 20S complex Deposited 2018-11-01 Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 12 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6MDM The 20S supercomplex engaging the SNAP-25 N-terminus (class 1) Deposited 2018-09-04 Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric（11） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 3.5 seconds before plunging.
Resolution 4.40 Å
6MDN The 20S supercomplex engaging the SNAP-25 N-terminus (class 2) Deposited 2018-09-04 Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric（11） Consistent with protein count
Chain A 1–723（723 aa）
Chain B 1–723（723 aa）
Chain C 1–723（723 aa）
Chain D 1–723（723 aa）
Chain E 1–723（723 aa）
Chain F 1–723（723 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 3.5 seconds before plunging.
Resolution 4.40 Å
6MDO The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 1) Deposited 2018-09-04 Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count
Chain A 1–723（723 aa）
Chain B 1–723（723 aa）
Chain C 1–723（723 aa）
Chain D 1–723（723 aa）
Chain E 1–723（723 aa）
Chain F 1–723（723 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 3.5 seconds before plunging.
Resolution 3.90 Å
6MDP The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 2) Deposited 2018-09-04 Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count
Chain A 1–723（723 aa）
Chain B 1–723（723 aa）
Chain C 1–723（723 aa）
Chain D 1–723（723 aa）
Chain E 1–723（723 aa）
Chain F 1–723（723 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 3.5 seconds before plunging.
Resolution 3.80 Å
9NV9 NSF Mg2+ class 1 heptamer Deposited 2025-03-20 Assembly 1 Protein homooligomer Homooligomer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Chain G 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9NVD NSF Mg2+ class 2 hexamer Deposited 2025-03-20 Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 10 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.70 Å
9OJR 21bin20S complex (NSF-alphaSNAP-2:1 syntaxin-1a:SNAP-25), non-hydrolyzing, class 3 Deposited 2025-05-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 11 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.95 Å
9OJU 21bin20S complex (NSF-alphaSNAP-2:1 syntaxin-1a:SNAP-25), non-hydrolyzing, class 4 Deposited 2025-05-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 10 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.97 Å
9OJZ 21bin20S complex (NSF-alphaSNAP-2:1 syntaxin-1a:SNAP-25), non-hydrolyzing, class 5 Deposited 2025-05-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 11 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.39 Å
9OK5 22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a:SNAP-25), hydrolyzing, class 16 Deposited 2025-05-09 Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 7 MG MAGNESIUM ION × 9 PO4 PHOSPHATE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.29 Å
9OKC 22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a:SNAP-25), hydrolyzing, class 17 Deposited 2025-05-09 Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 PO4 PHOSPHATE ION × 3 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.67 Å
9OLJ 22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a:SNAP-25), hydrolyzing, class 18 Deposited 2025-05-12 Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 7 MG MAGNESIUM ION × 10 PO4 PHOSPHATE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.52 Å
9OLO 22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a:SNAP-25), hydrolyzing, class 19 Deposited 2025-05-12 Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 7 MG MAGNESIUM ION × 10 PO4 PHOSPHATE ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.56 Å
9OMQ NSF, substrate free, hydrolyzing, class 24 Deposited 2025-05-14 Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 4 PO4 PHOSPHATE ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.86 Å
9PAF 21bin20S complex (NSF-alphaSNAP-2:1 syntaxin-1a:SNAP-25), non-hydrolyzing, class 6 Deposited 2025-06-25 Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 10 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
9PAG 21bin20S complex (NSF-alphaSNAP-2:1 syntaxin-1a:SNAP-25), non-hydrolyzing, class 7 Deposited 2025-06-25 Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 11 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.62 Å
9PB9 21bin20S complex (NSF-alphaSNAP-2:1 syntaxin-1a:SNAP-25), non-hydrolyzing, class 8 Deposited 2025-06-26 Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 11 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.45 Å
9PBA 21bin20S complex (NSF-alphaSNAP-2:1 syntaxin-1a:SNAP-25), non-hydrolyzing, class 9 Deposited 2025-06-26 Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 11 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.47 Å
9PBF 21bin20S complex (NSF-alphaSNAP-2:1 syntaxin-1a:SNAP-25), non-hydrolyzing, class 10 Deposited 2025-06-26 Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 10 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.01 Å
9PBV 21bin20S complex (NSF-alphaSNAP-2:1 syntaxin-1a:SNAP-25), non-hydrolyzing, class 11 Deposited 2025-06-26 Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 10 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.91 Å
9PC3 21bin20S complex (NSF-alphaSNAP-2:1 syntaxin-1a:SNAP-25), non-hydrolyzing, class 12 Deposited 2025-06-26 Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 11 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.69 Å
9PCX 22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a:SNAP-25), hydrolyzing, class 14 Deposited 2025-06-29 Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 7 PO4 PHOSPHATE ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.03 Å
9PCZ 22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a:SNAP-25), hydrolyzing, class 15 Deposited 2025-06-29 Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 7 MG MAGNESIUM ION × 10 PO4 PHOSPHATE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.65 Å
9PD1 22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a:SNAP-25), hydrolyzing, class 20 Deposited 2025-06-29 Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
9PD8 22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a:SNAP-25), hydrolyzing, class 21 Deposited 2025-06-30 Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric（15） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.23 Å
9PDB 22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a:SNAP-25), hydrolyzing, class 22 Deposited 2025-06-30 Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 5 PO4 PHOSPHATE ION × 3 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.83 Å
9PDD 22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a:SNAP-25), hydrolyzing, class 29 Deposited 2025-06-30 Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric（11） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 7 PO4 PHOSPHATE ION × 2 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.16 Å
9PF2 NSF, substrate free, hydrolyzing, class 25 Deposited 2025-07-03 Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 PO4 PHOSPHATE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.88 Å
9PFC NSF, substrate free, hydrolyzing, class 26 Deposited 2025-07-03 Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 7 PO4 PHOSPHATE ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.15 Å
9PFF Min22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a H3:SNAP-25 SN1), non-hydrolyzing, class 27 Deposited 2025-07-04 Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count
Chain A 1–744（744 aa）
Chain B 1–744（744 aa）
Chain C 1–744（744 aa）
Chain D 1–744（744 aa）
Chain E 1–744（744 aa）
Chain F 1–744（744 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 10 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.09 Å
9PFG Min22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a H3:SNAP-25 SN1), 4:2:2 alphaSNAP-syntaxin-1a H3-SNAP-25 SN1 subcomplex local refinement, non-hydrolyzing, class 28 Deposited 2025-07-04 Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric（10） Consistent with protein count
Chain J 1–744（744 aa）
Chain K 1–744（744 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.58 Å

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.