Current Protein Identity：P22681
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1B47 STRUCTURE OF THE N-TERMINAL DOMAIN OF CBL IN COMPLEX WITH ITS BINDING SITE IN ZAP-70 Deposited 1999-01-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
47–350（304 aa）
Fragment：N-TERMINAL DOMAIN
Chain B
47–350（304 aa）
Fragment：N-TERMINAL DOMAIN
Chain C
47–350（304 aa）
Fragment：N-TERMINAL DOMAIN
|
Not recorded | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.6；pH 5.6
|
Resolution 2.20 Å R-free 0.266 |
| 1FBV STRUCTURE OF A CBL-UBCH7 COMPLEX: RING DOMAIN FUNCTION IN UBIQUITIN-PROTEIN LIGASES Deposited 2000-07-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
47–434（388 aa）
|
Not recorded | ZN ZINC ION × 2 SO4 SULFATE ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；277 K；Sodium Citrate, Ammonium Sulfate,, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.90 Å R-free 0.262 |
| 1YVH Crystal Structure of the c-Cbl TKB Domain in Complex with the APS pTyr-618 Phosphopeptide Deposited 2005-02-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
23–351（329 aa）
Fragment：Tyrosine kinase binding domain, residues 25-351
|
Not recorded | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.1；277 K；PEG 8000, magnesium acetate, sodium cacodylate, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.05 Å R-free 0.247 |
| 2CBL N-TERMINAL DOMAIN OF CBL IN COMPLEX WITH ITS BINDING SITE ON ZAP-70 Deposited 1998-08-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
47–351（305 aa）
Fragment：DOMAIN
|
Not recorded | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.1；pH 6.1
|
Resolution 2.10 Å R-free 0.245 |
| 2JUJ Solution Structure of the UBA domain from c-Cbl Deposited 2007-08-30 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
851–906（56 aa）
Fragment：UBA domain
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 50；Pressure ambient
NMR sample composition
20mM sodium phosphate, 50mM sodium chloride, 1mM DTT, 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition
20mM sodium phosphate, 50mM sodium chloride, 1mM DTT, 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition
20mM sodium phosphate, 50mM sodium chloride, 1mM DTT, 100% D2O | 100% D2O
|
Resolution not provided |
| 2K4D E2-c-Cbl recognition is necessary but not sufficient for ubiquitination activity Deposited 2008-06-06 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
358–437（80 aa）
Fragment：RING domain, residues 358-437
|
Not recorded | ZN ZINC ION × 2 | SOLUTION NMR |
NMR measurement conditions
pH 7.3；298 K；Ionic strength (raw mmCIF value) 150；Pressure ambient
NMR sample composition
1mM [U-99% 15N] c-Cbl; 50mM potassium phosphate; 100mM sodium chloride; 0.100mM zinc chloride; 0.2mM PMSF; 5mM DTT; 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
1mM [U-99% 13C; U-99% 15N] c-Cbl; 50mM potassium phosphate; 100mM sodium chloride; 0.100mM zinc chloride; 0.2mM PMSF; 5mM DTT; 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 2OO9 crystal structure of the UBA domain from human c-Cbl ubiquitin ligase Deposited 2007-01-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
856–895（40 aa）
Fragment：UBA domain
Chain B
856–895（40 aa）
Fragment：UBA domain
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；3.8M sodium formate, 0.1M Tris, 4% (v/v) glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.10 Å R-free 0.260 |
| 2OO9 crystal structure of the UBA domain from human c-Cbl ubiquitin ligase Deposited 2007-01-25 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
856–895（40 aa）
Fragment：UBA domain
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；3.8M sodium formate, 0.1M Tris, 4% (v/v) glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.10 Å R-free 0.260 |
| 2Y1M Structure of native c-Cbl Deposited 2010-12-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
47–435（389 aa）
Fragment：RESIDUES 47-435
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 9；0.1 M TRIS-HCL, PH 9.0-9.5, 3.7-4.0 M SODIUM FORMATE, 5 MM DTT
|
Resolution 2.67 Å R-free 0.263 |
| 2Y1M Structure of native c-Cbl Deposited 2010-12-08 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
47–435（389 aa）
Fragment：RESIDUES 47-435
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 9；0.1 M TRIS-HCL, PH 9.0-9.5, 3.7-4.0 M SODIUM FORMATE, 5 MM DTT
|
Resolution 2.67 Å R-free 0.263 |
| 2Y1M Structure of native c-Cbl Deposited 2010-12-08 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
47–435（389 aa）
Fragment：RESIDUES 47-435
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 9；0.1 M TRIS-HCL, PH 9.0-9.5, 3.7-4.0 M SODIUM FORMATE, 5 MM DTT
|
Resolution 2.67 Å R-free 0.263 |
| 2Y1M Structure of native c-Cbl Deposited 2010-12-08 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
47–435（389 aa）
Fragment：RESIDUES 47-435
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 9；0.1 M TRIS-HCL, PH 9.0-9.5, 3.7-4.0 M SODIUM FORMATE, 5 MM DTT
|
Resolution 2.67 Å R-free 0.263 |
| 2Y1M Structure of native c-Cbl Deposited 2010-12-08 | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain E
47–435（389 aa）
Fragment：RESIDUES 47-435
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 9；0.1 M TRIS-HCL, PH 9.0-9.5, 3.7-4.0 M SODIUM FORMATE, 5 MM DTT
|
Resolution 2.67 Å R-free 0.263 |
| 2Y1M Structure of native c-Cbl Deposited 2010-12-08 | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain F
47–435（389 aa）
Fragment：RESIDUES 47-435
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 9；0.1 M TRIS-HCL, PH 9.0-9.5, 3.7-4.0 M SODIUM FORMATE, 5 MM DTT
|
Resolution 2.67 Å R-free 0.263 |
| 2Y1N Structure of c-Cbl-ZAP-70 peptide complex Deposited 2010-12-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
47–435（389 aa）
Fragment：RESIDUES 47-435
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
18-20% (W/V) PEG 3350, 0.2 M AMMONIUM FORMATE
|
Resolution 2.00 Å R-free 0.180 |
| 2Y1N Structure of c-Cbl-ZAP-70 peptide complex Deposited 2010-12-08 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
47–435（389 aa）
Fragment：RESIDUES 47-435
|
Not recorded | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
18-20% (W/V) PEG 3350, 0.2 M AMMONIUM FORMATE
|
Resolution 2.00 Å R-free 0.180 |
| 3BUM Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Sprouty2 Deposited 2008-01-03 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
25–351（327 aa）
Fragment：c-Cbl TKB domain, UNP residues 25-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；ammonium sulfate, PEG3350, pH7.5, vapor diffusion, hanging drop, temperature 298K, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.00 Å R-free 0.263 |
| 3BUM Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Sprouty2 Deposited 2008-01-03 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
25–351（327 aa）
Fragment：c-Cbl TKB domain, UNP residues 25-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；ammonium sulfate, PEG3350, pH7.5, vapor diffusion, hanging drop, temperature 298K, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.00 Å R-free 0.263 |
| 3BUN Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Sprouty4 Deposited 2008-01-03 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
23–351（329 aa）
Fragment：c-Cbl TKB domain, CBL N-terminal, UNP residues 23-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；0.2M NA/K TARTRATE, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.242 |
| 3BUN Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Sprouty4 Deposited 2008-01-03 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
23–351（329 aa）
Fragment：c-Cbl TKB domain, CBL N-terminal, UNP residues 23-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；0.2M NA/K TARTRATE, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.242 |
| 3BUO Crystal structure of c-Cbl-TKB domain complexed with its binding motif in EGF receptor' Deposited 2008-01-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
23–351（329 aa）
Fragment：c-Cbl TKB domain, CBL N-terminal, UNP residues 23-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；0.25M Na formate, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.60 Å R-free 0.278 |
| 3BUO Crystal structure of c-Cbl-TKB domain complexed with its binding motif in EGF receptor' Deposited 2008-01-03 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
23–351（329 aa）
Fragment：c-Cbl TKB domain, CBL N-terminal, UNP residues 23-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；0.25M Na formate, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.60 Å R-free 0.278 |
| 3BUW Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Syk Deposited 2008-01-03 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
23–351（329 aa）
Fragment：c-Cbl TKB domain, CBL N-terminal, UNP residues 23-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.12M ammonium acetate, 18% PEG 3350, pH 6.5, vapor diffusion, hanging drop, temperature 298K
|
Resolution 1.45 Å R-free 0.238 |
| 3BUW Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Syk Deposited 2008-01-03 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
23–351（329 aa）
Fragment：c-Cbl TKB domain, CBL N-terminal, UNP residues 23-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.12M ammonium acetate, 18% PEG 3350, pH 6.5, vapor diffusion, hanging drop, temperature 298K
|
Resolution 1.45 Å R-free 0.238 |
| 3BUW Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Syk Deposited 2008-01-03 | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
23–351（329 aa）
Fragment：c-Cbl TKB domain, CBL N-terminal, UNP residues 23-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.12M ammonium acetate, 18% PEG 3350, pH 6.5, vapor diffusion, hanging drop, temperature 298K
|
Resolution 1.45 Å R-free 0.238 |
| 3BUW Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Syk Deposited 2008-01-03 | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
23–351（329 aa）
Fragment：c-Cbl TKB domain, CBL N-terminal, UNP residues 23-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.12M ammonium acetate, 18% PEG 3350, pH 6.5, vapor diffusion, hanging drop, temperature 298K
|
Resolution 1.45 Å R-free 0.238 |
| 3BUX Crystal structure of c-Cbl-TKB domain complexed with its binding motif in c-Met Deposited 2008-01-03 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
23–351（329 aa）
Fragment：c-Cbl TKB domain, CBL N-terminal, UNP residues 23-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；0.15M malic acid, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.35 Å R-free 0.240 |
| 3BUX Crystal structure of c-Cbl-TKB domain complexed with its binding motif in c-Met Deposited 2008-01-03 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
23–351（329 aa）
Fragment：c-Cbl TKB domain, CBL N-terminal, UNP residues 23-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；0.15M malic acid, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.35 Å R-free 0.240 |
| 3BUX Crystal structure of c-Cbl-TKB domain complexed with its binding motif in c-Met Deposited 2008-01-03 | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
23–351（329 aa）
Fragment：c-Cbl TKB domain, CBL N-terminal, UNP residues 23-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；0.15M malic acid, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.35 Å R-free 0.240 |
| 3BUX Crystal structure of c-Cbl-TKB domain complexed with its binding motif in c-Met Deposited 2008-01-03 | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
23–351（329 aa）
Fragment：c-Cbl TKB domain, CBL N-terminal, UNP residues 23-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；0.15M malic acid, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.35 Å R-free 0.240 |
| 3OB1 Crystal structure of c-Cbl TKB domain in complex with double phosphorylated Spry2 peptide Deposited 2010-08-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
25–351（327 aa）
Fragment：c-Cbl TKB domain, residues 25-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；20% PEG 3350, 0.2M K/Na tartrate, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.20 Å R-free 0.223 |
| 3OB2 Crystal structure of c-Cbl TKB domain in complex with double phosphorylated EGFR peptide Deposited 2010-08-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
25–351（327 aa）
Fragment：c-Cbl TKB domain, residues 25-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.1；298 K；20% (w/v) PEG 3350, 150mM NaK tartrate, 0.1 M Bis-Tris propane pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.10 Å R-free 0.217 |
| 3PLF Reverse Binding Mode of MetRD peptide complexed with c-Cbl TKB domain Deposited 2010-11-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
25–351（327 aa）
Fragment：TKB domain
|
Not recorded | CA CALCIUM ION × 1 CL CHLORIDE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
hanging drop；pH 6.5；298 K；100mM Bis-tris propane, 50mM ammonium sulfate, pH 6.5, hanging drop, temperature 298K
|
Resolution 1.92 Å R-free 0.180 |
| 3PLF Reverse Binding Mode of MetRD peptide complexed with c-Cbl TKB domain Deposited 2010-11-15 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
25–351（327 aa）
Fragment：TKB domain
|
Not recorded | CA CALCIUM ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
hanging drop；pH 6.5；298 K；100mM Bis-tris propane, 50mM ammonium sulfate, pH 6.5, hanging drop, temperature 298K
|
Resolution 1.92 Å R-free 0.180 |
| 4A49 Structure of phosphoTyr371-c-Cbl-UbcH5B complex Deposited 2011-10-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
354–435（82 aa）
Fragment：C-CBL RESIDUES 354-435
|
Mutation：Y368F Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 2 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
291 K；20% (W/V) PEG 3350 AND 0.2 M POTASSIUM THIOCYANATE AT 18 DEGREES CELSIUS.
|
Resolution 2.21 Å R-free 0.205 |
| 4A4B Structure of modified phosphoTyr371-c-Cbl-UbcH5B-ZAP-70 complex Deposited 2011-10-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
47–435（389 aa）
Fragment：TKB DOMAIN, LINKER HELIX REGION, AND RING DOMAIN, RESIDUES 47-435
|
Mutation：YES Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；31-35% PENTAERYTHRITOL PROPOXYLATE (5/4 PO/OH), 0.2 M KCL, 50 MM HEPES, PH 7.5
|
Resolution 2.79 Å R-free 0.267 |
| 4A4C Structure of phosphoTyr371-c-Cbl-UbcH5B-ZAP-70 complex Deposited 2011-10-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
47–435（389 aa）
Fragment：TKB DOMAIN, LINKER HELIX REGION, AND RING DOMAIN, RESIDUES 47-435
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
277 K；50 MM HEPES, PH 7.5, 0.2 M KCL AND 31-35% (V/V) PENTAERYTHRITOL PROPOXYLATE (5/4 PO/OH) AT 4 DEGREES CELSIUS.
|
Resolution 2.70 Å R-free 0.268 |
| 4GPL Structure of Cbl(TKB) bound to a phosphorylated pentapeptide Deposited 2012-08-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
47–351（305 aa）
Fragment：human Cbl TKB domain residues 47-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.25M potassium sodium tartrate tetrahydrate, 20% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.00 Å R-free 0.248 |
| 4GPL Structure of Cbl(TKB) bound to a phosphorylated pentapeptide Deposited 2012-08-21 | Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
47–351（305 aa）
Fragment：human Cbl TKB domain residues 47-351
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.25M potassium sodium tartrate tetrahydrate, 20% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.00 Å R-free 0.248 |
| 5HKW Crystal Structure of Apo c-Cbl TKBD Refined to 2.25 A Resolution Deposited 2016-01-14 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
47–351（305 aa）
Fragment：Tyrosine kinase binding domain (TKBD), residues 47-351
|
Not recorded | NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% (w/v) PEG 3350, 0.2M sodium citrate dibasic trihyrate
|
Resolution 2.25 Å R-free 0.234 |
| 5HKW Crystal Structure of Apo c-Cbl TKBD Refined to 2.25 A Resolution Deposited 2016-01-14 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
47–351（305 aa）
Fragment：Tyrosine kinase binding domain (TKBD), residues 47-351
|
Not recorded | NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% (w/v) PEG 3350, 0.2M sodium citrate dibasic trihyrate
|
Resolution 2.25 Å R-free 0.234 |
| 5HKW Crystal Structure of Apo c-Cbl TKBD Refined to 2.25 A Resolution Deposited 2016-01-14 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
47–351（305 aa）
Fragment：Tyrosine kinase binding domain (TKBD), residues 47-351
|
Not recorded | NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% (w/v) PEG 3350, 0.2M sodium citrate dibasic trihyrate
|
Resolution 2.25 Å R-free 0.234 |
| 5HKX Crystal Structure of c-Cbl TKBD-RING domains (Y371E mutant) Refined to 1.85 A Resolution Deposited 2016-01-14 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
47–435（389 aa）
Fragment：Tyrosine kinase binding and RING domains (TKBD-RING), residues 47-435
|
Mutation：Y371E | EDO 1,2-ETHANEDIOL × 1 ZN ZINC ION × 2 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；10% (w/v) PEG 5000 MME, 0.1M MES, 12% (v/v) 1-propanol
|
Resolution 1.85 Å R-free 0.214 |
| 5HKY Crystal structure of c-Cbl TKBD domain in complex with SPRY2 peptide (36-60, pY55) Refined to 1.8A Resolution (P6 form) Deposited 2016-01-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
47–351（305 aa）
Fragment：Tyrosine kinase binding domain (TKBD), residues 47-351
|
Not recorded | CL CHLORIDE ION × 2 NA SODIUM ION × 1 1PE PENTAETHYLENE GLYCOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；30% (v/v) PEG 400, 0.1M HEPES, 200 mM MgCl2
|
Resolution 1.80 Å R-free 0.180 |
| 5HKZ Crystal Structure of c-Cbl TKBD in complex with SPRY2 peptide (36-60, pY55) Refined to 1.8 A Resolution (P21 form) Deposited 2016-01-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
47–351（305 aa）
Fragment：Tyrosine kinase binding domain (TKBD), residues 47-351
|
Not recorded | NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；25% (w/v) PEG 2000 MME, 0.1M HEPES
|
Resolution 1.80 Å R-free 0.213 |
| 5HL0 Crystal Structure of c-Cbl TKBD in complex with SPRY2 peptide (54-60, pY55) Refined to 2.2A Resolution Deposited 2016-01-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
47–351（305 aa）
|
Not recorded | NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；16% (w/v) PEG 8000, 0.04M Potassium phosphate (monobasic), 20% (v/v) glycerol
|
Resolution 2.20 Å R-free 0.237 |
| 5J3X Structure of c-CBL Y371F Deposited 2016-03-31 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
47–435（389 aa）
Fragment：UNP residues 47-435
|
Mutation：Y371F | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；281.15 K；Equal volume protein at 10 mg/mL + well containing 3.0-3.1 M sodium formate
0.1 M Tris-HCl, pH 8.5
5 mM DTT
|
Resolution 2.82 Å R-free 0.254 |
| 5J3X Structure of c-CBL Y371F Deposited 2016-03-31 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
47–435（389 aa）
Fragment：UNP residues 47-435
|
Mutation：Y371F | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；281.15 K；Equal volume protein at 10 mg/mL + well containing 3.0-3.1 M sodium formate
0.1 M Tris-HCl, pH 8.5
5 mM DTT
|
Resolution 2.82 Å R-free 0.254 |
| 5J3X Structure of c-CBL Y371F Deposited 2016-03-31 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
47–435（389 aa）
Fragment：UNP residues 47-435
|
Mutation：Y371F | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；281.15 K；Equal volume protein at 10 mg/mL + well containing 3.0-3.1 M sodium formate
0.1 M Tris-HCl, pH 8.5
5 mM DTT
|
Resolution 2.82 Å R-free 0.254 |
| 5J3X Structure of c-CBL Y371F Deposited 2016-03-31 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
47–435（389 aa）
Fragment：UNP residues 47-435
|
Mutation：Y371F | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；281.15 K；Equal volume protein at 10 mg/mL + well containing 3.0-3.1 M sodium formate
0.1 M Tris-HCl, pH 8.5
5 mM DTT
|
Resolution 2.82 Å R-free 0.254 |
| 5J3X Structure of c-CBL Y371F Deposited 2016-03-31 | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain E
47–435（389 aa）
Fragment：UNP residues 47-435
|
Mutation：Y371F | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；281.15 K；Equal volume protein at 10 mg/mL + well containing 3.0-3.1 M sodium formate
0.1 M Tris-HCl, pH 8.5
5 mM DTT
|
Resolution 2.82 Å R-free 0.254 |
| 5J3X Structure of c-CBL Y371F Deposited 2016-03-31 | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain F
47–435（389 aa）
Fragment：UNP residues 47-435
|
Mutation：Y371F | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；281.15 K；Equal volume protein at 10 mg/mL + well containing 3.0-3.1 M sodium formate
0.1 M Tris-HCl, pH 8.5
5 mM DTT
|
Resolution 2.82 Å R-free 0.254 |
| 5O76 Structure of phosphoY371 c-CBL in complex with ZAP70-peptide and UbV.pCBL ubiquitin variant Deposited 2017-06-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
47–435（389 aa）
|
Mutation：Y368F Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.1 M monosaccharides, 0.1 M Buffer 1 pH 6.5, 50% Precipitant 2, Morpheus screen
|
Resolution 2.47 Å R-free 0.248 |
| 5O76 Structure of phosphoY371 c-CBL in complex with ZAP70-peptide and UbV.pCBL ubiquitin variant Deposited 2017-06-08 | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
47–435（389 aa）
|
Mutation：Y368F Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.1 M monosaccharides, 0.1 M Buffer 1 pH 6.5, 50% Precipitant 2, Morpheus screen
|
Resolution 2.47 Å R-free 0.248 |
| 6O02 Monobody (MC3) bound to tyrosine kinase binding domain of E3 ubiquitin ligase CBL Deposited 2019-02-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
47–353（307 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；0.12M Alcohols, 0.1M Buffer system 3 at PH 8.5 and 50% v/v Precipitant mix 2
|
Resolution 2.95 Å R-free 0.258 |
| 6O03 Monobody (MC17) bound to tyrosine kinase binding domain of E3 ubiquitin ligase CBL Deposited 2019-02-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
47–353（307 aa）
|
Mutation：G306E | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；20% (w/v) PEG 3000, 100mM tris base/hydrochloric acid pH 7, 200mM calcium acetate
|
Resolution 3.30 Å R-free 0.330 |
| 6O03 Monobody (MC17) bound to tyrosine kinase binding domain of E3 ubiquitin ligase CBL Deposited 2019-02-15 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
47–353（307 aa）
|
Mutation：G306E | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；20% (w/v) PEG 3000, 100mM tris base/hydrochloric acid pH 7, 200mM calcium acetate
|
Resolution 3.30 Å R-free 0.330 |
| 6XAR Structure of CBL tyrosine kinase binding domain (TKBD) with C-terminal tail of Src-like kinase protein 2 (SLAP2) Deposited 2020-06-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
25–357（333 aa）
|
Not recorded | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；CBL/SLAP2 complex mixed in hanging drop with equal volume (200 nL) of 0.1 M Hepes pH 7.5, 10% (w/v) PEG8000
|
Resolution 2.50 Å R-free 0.293 |
| 6XAR Structure of CBL tyrosine kinase binding domain (TKBD) with C-terminal tail of Src-like kinase protein 2 (SLAP2) Deposited 2020-06-04 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
25–357（333 aa）
|
Not recorded | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；CBL/SLAP2 complex mixed in hanging drop with equal volume (200 nL) of 0.1 M Hepes pH 7.5, 10% (w/v) PEG8000
|
Resolution 2.50 Å R-free 0.293 |
| 7SIY cCBL TKB domain in complex with pZAP70 peptide Deposited 2021-10-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
48–351（304 aa）
|
Not recorded | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.2 M Ammonium formate, 20% PEG3350
|
Resolution 1.48 Å R-free 0.221 |
| 9ERZ Structure of CBL-TKBD bound to Ubiquitin-fused CBLock peptide Deposited 2024-03-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
47–355（309 aa）
Chain C
47–355（309 aa）
|
Not recorded | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.1 M Bis-Tris Propane, pH 6.0, 0.2 M sodium fluoride, and 20% (v/v) PEG 3350
|
Resolution 2.02 Å R-free 0.229 |