Current Protein Identity:P32605
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 5ZWN Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.3 angstrom (Part II: U1 snRNP region) Deposited 2018-05-16 | Assembly 1 Protein–RNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain S
1–298(298 aa)
|
Not recorded | ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 | Assembly 1 Protein–RNA Heteromer;Protein × 35 PDB declaration: 38-meric(38) Consistent with all polymers |
Chain A
1–118(118 aa)
Chain A
149–298(150 aa)
|
Not recorded | ZN ZINC ION × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE;Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
|
Resolution 4.00 Å |
| 6N7P S. cerevisiae spliceosomal E complex (UBC4) Deposited 2018-11-27 | Assembly 1 Protein–RNA Heteromer;Protein × 19 PDB declaration: 21-meric(21) Consistent with all polymers |
Chain C
1–298(298 aa)
|
Not recorded | ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6N7R Saccharomyces cerevisiae spliceosomal E complex (ACT1) Deposited 2018-11-28 | Assembly 1 Protein–RNA Heteromer;Protein × 16 PDB declaration: octadecameric(18) Consistent with all polymers |
Chain C
1–298(298 aa)
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6N7X S. cerevisiae U1 snRNP Deposited 2018-11-28 | Assembly 1 Protein–RNA Heteromer;Protein × 15 PDB declaration: hexadecameric(16) Consistent with all polymers |
Chain C
1–298(298 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 7OQC The U1 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 | Assembly 1 Protein–RNA Heteromer;Protein × 16 PDB declaration: octadecameric(18) Consistent with all polymers |
Chain A
1–298(298 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 | Assembly 1 Protein–RNA Heteromer;Protein × 36 PDB declaration: 39-meric(39) Consistent with all polymers |
Chain A
1–298(298 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.90 Å |
| 8W2O Yeast U1 snRNP with humanized U1C Zinc-Finger domain Deposited 2024-02-20 | Assembly 1 Protein–RNA Heteromer;Protein × 16 PDB declaration: 18-meric(18) Consistent with all polymers |
Chain C
1–298(298 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9;20 mM Hepes, pH7.9, 120 mM KCl, 2 mM EGTA
cryo-EM vitrification conditions
Cryogen ETHANE;The grids were obtained with the chamber at 100% humidity, 2.5 s blotting time, -6 blotting force and 15 s wait time and flash-frozen into liquid ethane with a Vitrobot Mark IV (Thermo Fisher Scientific)
|
Resolution 3.49 Å |