Current Protein Identity:P36070
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3TJ1 Crystal Structure of RNA Polymerase I Transcription Initiation Factor Rrn3 Deposited 2011-08-23 | Assembly 1 Protein homooligomer Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
1–627(627 aa)
Chain B
1–627(627 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7;293 K;14% PEG 3350, 250 mM sodium-potassium-tartrate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.85 Å R-free 0.242 |
| 5G5L RNA polymerase I-Rrn3 complex at 4.8 A resolution Deposited 2016-05-26 | Assembly 1 Protein heterocomplex Heteromer;Protein × 15 PDB declaration: pentadecameric(15) Consistent with protein count |
Chain O
1–627(627 aa)
|
Not recorded | ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
150MM NACL, 5MM HEPES, 1MM MGCL2, 0.01 MM ZNCL2, 5 MM DTT;pH 7.8;150MM NACL, 5MM HEPES, 1MM MGCL2, 0.01 MM ZNCL2, 5 MM DTT
cryo-EM vitrification conditions
Cryogen ETHANE;VITROBOT MARK IV, METHOD- 4.5 MICROLITERS OF SAMPLE WAS APPLIED TO GLOW- DISCHARGED QUANTIFOIL R 2- 1 HOLEY CARBON GRIDS, WHICH WERE THEN BLOTTED FOR 8.5S AND PLUNGE- FROZEN IN LIQUID ETHANE.
|
Resolution 4.80 Å |
| 5N5Y Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation III) Deposited 2017-02-14 | Assembly 1 Protein heterocomplex Heteromer;Protein × 18 PDB declaration: octadecameric(18) Consistent with protein count |
Chain O
1–627(627 aa)
|
Not recorded | ZN ZINC ION × 8 SO4 SULFATE ION × 5 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.70 Å |
| 5N5Z Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation II) Deposited 2017-02-14 | Assembly 1 Protein heterocomplex Heteromer;Protein × 18 PDB declaration: octadecameric(18) Consistent with protein count |
Chain O
1–627(627 aa)
|
Not recorded | ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.70 Å |
| 5N60 Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation I) Deposited 2017-02-14 | Assembly 1 Protein heterocomplex Heteromer;Protein × 18 PDB declaration: octadecameric(18) Consistent with protein count |
Chain O
1–627(627 aa)
|
Not recorded | ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.70 Å |
| 5N61 RNA polymerase I initially transcribing complex Deposited 2017-02-14 | Assembly 1 Other combination Heteromer;Protein × 18 PDB declaration: 21-meric(21) Consistent with all polymers |
Chain O
1–627(627 aa)
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 5OA1 RNA polymerase I pre-initiation complex Deposited 2017-06-20 | Assembly 1 Protein–DNA Heteromer;Protein × 32 PDB declaration: 34-meric(34) Consistent with all polymers |
Chain O
1–627(627 aa)
|
Not recorded | ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å |
| 6RQH RNA Polymerase I Closed Conformation 1 (CC1) Deposited 2019-05-15 | Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain O
1–627(627 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6RQL RNA Polymerase I Closed Conformation 2 (CC2) Deposited 2019-05-16 | Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain O
1–627(627 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 6RQT RNA Polymerase I-tWH-Rrn3-DNA Deposited 2019-05-16 | Assembly 1 Protein–DNA Heteromer;Protein × 15 PDB declaration: heptadecameric(17) Consistent with all polymers |
Chain O
1–627(627 aa)
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 6RRD RNA Polymerase I Pre-initiation complex DNA opening intermediate 1 Deposited 2019-05-17 | Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain O
1–627(627 aa)
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6RUI RNA Polymerase I Pre-initiation complex DNA opening intermediate 2 Deposited 2019-05-28 | Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain O
1–627(627 aa)
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 6RUO RNA Polymerase I Open Complex conformation 1 Deposited 2019-05-28 | Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain O
1–627(627 aa)
|
Not recorded | ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6RWE RNA Polymerase I Open Complex conformation 2 Deposited 2019-06-04 | Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain O
1–627(627 aa)
|
Not recorded | ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 6TPS early intermediate RNA Polymerase I Pre-initiation complex - eiPIC Deposited 2019-12-14 | Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: 22-meric(22) Consistent with all polymers |
Chain O
1–627(627 aa)
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.54 Å |