5n5z

Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation II)

Method: ELECTRON MICROSCOPY

▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-directed RNA polymerase I subunit RPA190

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPA1_YEAST
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–1664； UniProt 1–1664

DNA-directed RNA polymerase I subunit RPA135

OrganismNot specified

UniProt P22138

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPA2_YEAST
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–1203； UniProt 1–1203

DNA-directed RNA polymerases I and III subunit RPAC1

OrganismNot specified

UniProt P07703

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAC1_YEAST
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–335； UniProt 1–335

DNA-directed RNA polymerase I subunit RPA14

OrganismNot specified

UniProt P50106

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPA14_YEAST
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain D； PDBConstruct 1–137； UniProt 1–137

DNA-directed RNA polymerases I, II, and III subunit RPABC1

OrganismNot specified

UniProt P20434

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB1_YEAST
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain E； PDBConstruct 1–215； UniProt 1–215

DNA-directed RNA polymerases I, II, and III subunit RPABC2

OrganismNot specified

UniProt P20435

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB2_YEAST
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain F； PDBConstruct 1–155； UniProt 1–155

DNA-directed RNA polymerase I subunit RPA43

OrganismNot specified

UniProt P46669

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPA43_YEAST
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain G； PDBConstruct 1–326； UniProt 1–326

DNA-directed RNA polymerases I, II, and III subunit RPABC3

OrganismNot specified

UniProt P20436

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB3_YEAST
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain H； PDBConstruct 1–146； UniProt 1–146

DNA-directed RNA polymerase I subunit RPA12

OrganismNot specified

UniProt P32529

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPA12_YEAST
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain I； PDBConstruct 1–125； UniProt 1–125

DNA-directed RNA polymerases I, II, and III subunit RPABC5

OrganismNot specified

UniProt P22139

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB5_YEAST
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain J； PDBConstruct 1–70； UniProt 1–70

DNA-directed RNA polymerases I and III subunit RPAC2

OrganismNot specified

UniProt P28000

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAC2_YEAST
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain K； PDBConstruct 1–142； UniProt 1–142

DNA-directed RNA polymerases I, II, and III subunit RPABC4

OrganismNot specified

UniProt P40422

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB4_YEAST
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain L； PDBConstruct 1–70； UniProt 1–70

DNA-directed RNA polymerase I subunit RPA49

OrganismNot specified

UniProt Q01080

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPA49_YEAST
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain M； PDBConstruct 1–415； UniProt 1–415

DNA-directed RNA polymerase I subunit RPA34

OrganismNot specified

UniProt P47006

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPA34_YEAST
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain N； PDBConstruct 1–233； UniProt 1–233

RNA polymerase I-specific transcription initiation factor RRN3

Saccharomyces cerevisiae

UniProt P36070

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RRN3_YEAST
Isoform	—
PDB entities	15
Chains and sequence ranges	Author chain O； PDBConstruct 1–627； UniProt 1–627

RNA polymerase I-specific transcription initiation factor RRN6

Saccharomyces cerevisiae

UniProt P32786

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RRN6_YEAST
Isoform	—
PDB entities	16
Chains and sequence ranges	Author chain P； PDBConstruct 1–894； UniProt 1–894

RNA polymerase I-specific transcription initiation factor RRN7

Saccharomyces cerevisiae

UniProt P40992

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN11 × 1 （Q04712） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RRN7_YEAST
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain Q； PDBConstruct 1–514； UniProt 1–514

RNA polymerase I-specific transcription initiation factor RRN11

Saccharomyces cerevisiae

UniProt Q04712

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	DNA-directed RNA polymerase I subunit RPA190 × 1 （P10964） DNA-directed RNA polymerase I subunit RPA135 × 1 （P22138） DNA-directed RNA polymerases I and III subunit RPAC1 × 1 （P07703） DNA-directed RNA polymerase I subunit RPA14 × 1 （P50106） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P20434） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P20435） DNA-directed RNA polymerase I subunit RPA43 × 1 （P46669） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P20436） DNA-directed RNA polymerase I subunit RPA12 × 1 （P32529） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P22139） DNA-directed RNA polymerases I and III subunit RPAC2 × 1 （P28000） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P40422） DNA-directed RNA polymerase I subunit RPA49 × 1 （Q01080） DNA-directed RNA polymerase I subunit RPA34 × 1 （P47006） RNA polymerase I-specific transcription initiation factor RRN3 × 1 （P36070） RNA polymerase I-specific transcription initiation factor RRN6 × 1 （P32786） RNA polymerase I-specific transcription initiation factor RRN7 × 1 （P40992） ZINC ION × 8	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RRN11_YEAST
Isoform	—
PDB entities	18
Chains and sequence ranges	Author chain R； PDBConstruct 1–507； UniProt 1–507

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id	5n5z
Deposition date deposition_date	2017-02-14
Structure title title	Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation II)
Keywords keywords	Transcription, RNA polymerase I initiation; TRANSCRIPTION
Experimental Method method	ELECTRON MICROSCOPY

▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

5n5z__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

5n5z__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

5n5z__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	63.52 Å
Rg (electron density)	63.14 Å
Total Rg	63.15 Å
Atom count	48004
Residues	5973
Excluded volume	858010 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`5n5z__assembly_1__model_1`	octadecameric （18）	Success	4.1.3-1-20251215 (887e7ef)	View Download

▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (19)

Entity 1 Entity 1 polymer

Description description	DNA-directed RNA polymerase I subunit RPA190
Molecular weight formula_weight	186677.0 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	48004

MDISKPVGSEITSVDFGILTAKEIRNLSAKQITNPTVLDNLGHPVSGGLYDLALGAFLRNLCSTCGLDEKFCPGHQGHIELPVPCYNPLFFNQLYIYLRASCLFCHHFRLKSVEVHRYACKLRLLQYGLIDESYKLDEITLGSLNSSMYTDDEAIEDNEDEMDGEGSKQSKDISSTLLNELKSKRSEYVDMAIAKALSDGRTTERGSFTATVNDERKKLVHEFHKKLLSRGKCDNCGMFSPKFRKDGFTKIFETALNEKQITNNRVKGFIRQDMIKKQKQAKKLDGSNEASANDEESFDVGRNPTTRPKTGSTYILSTEVKNILDTVFRKEQCVLQYVFHSRPNLSRKLVKADSFFMDVLVVPPTRFRLPSKLGEEVHENSQNQLLSKVLTTSLLIRDLNDDLSKLQKDKVSLEDRRVIFSRLMNAFVTIQNDVNAFIDSTKAQGRTSGKVPIPGVKQALEKKEGLFRKHMMGKRVNYAARSVISPDPNIETNEIGVPPVFAVKLTYPEPVTAYNIAELRQAVINGPDKWPGATQIQNEDGSLVSLIGMSVEQRKALANQLLTPSSNVSTHTLNKKVYRHIKNRDVVLMNRQPTLHKASMMGHKVRVLPNEKTLRLHYANTGAYNADFDGDEMNMHFPQNENARAEALNLANTDSQYLTPTSGSPVRGLIQDHISAGVWLTSKDSFFTREQYQQYIYGCIRPEDGHTTRSKIVTLPPTIFKPYPLWTGKQIITTVLLNVTPPDMPGINLISKNKIKNEYWGKGSLENEVLFKDGALLCGILDKSQYGASKYGIVHSLHEVYGPEVAAKVLSVLGRLFTNYITATAFTCGMDDLRLTAEGNKWRTDILKTSVDTGREAAAEVTNLDKDTPADDPELLKRLQEILRDNNKSGILDAVTSSKVNAITSQVVSKCVPDGTMKKFPCNSMQAMALSGAKGSNVNVSQIMCLLGQQALEGRRVPVMVSGKTLPSFKPYETDAMAGGYVKGRFYSGIKPQEYYFHCMAGREGLIDTAVKTSRSGYLQRCLTKQLEGVHVSYDNSIRDADGTLVQFMYGGDAIDITKESHMTQFEFCLDNYYALLKKYNPSALIEHLDVESALKYSKKTLKYRKKHSKEPHYKQSVKYDPVLAKYNPAKYLGSVSENFQDKLESFLDKNSKLFKSSDGVNEKKFRALMQLKYMRSLINPGEAVGIIASQSVGEPSTQMTLNTFHFAGHGAANVTLGIPRLREIVMTASAAIKTPQMTLPIWNDVSDEQADTFCKSISKVLLSEVIDKVIVTETTGTSNTAGGNAARSYVIHMRFFDNNEYSEEYDVSKEELQNVISNQFIHLLEAAIVKEIKKQKRTTGPDIGVAVPRLQTDVANSSSNSKRLEEDNDEEQSHKKTKQAVSYDEPDEDEIETMREAEKSSDEEGIDSDKESDSDSEDEDVDMNEQINKSIVEANNNMNKVQRDRQSAIISHHRFITKYNFDDESGKWCEFKLELAADTEKLLMVNIVEEICRKSIIRQIPHIDRCVHPEPENGKRVLVTEGVNFQAMWDQEAFIDVDGITSNDVAAVLKTYGVEAARNTIVNEINNVFSRYAISVSFRHLDLIADMMTRQGTYLAFNRQGMETSTSSFMKMSYETTCQFLTKAVLDNEREQLDSPSARIVVGKLNNVGTGSFDVLAKVPNAA

Entity 2 Entity 2 polymer

Description description	DNA-directed RNA polymerase I subunit RPA135
Molecular weight formula_weight	135910.3 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

MSKVIKPPGQARTADFRTLERESRFINPPKDKSAFPLLQEAVQPHIGSFNALTEGPDGGLLNLGVKDIGEKVIFDGKPLNSEDEISNSGYLGNKLSVSVEQVSIAKPMSNDGVSSAVERKVYPSESRQRLTSYRGKLLLKLKWSVNNGEENLFEVRDCGGLPVMLQSNRCHLNKMSPYELVQHKEESDEIGGYFIVNGIEKLIRMLIVQRRNHPMAIIRPSFANRGASYSHYGIQIRSVRPDQTSQTNVLHYLNDGQVTFRFSWRKNEYLVPVVMILKALCHTSDREIFDGIIGNDVKDSFLTDRLELLLRGFKKRYPHLQNRTQVLQYLGDKFRVVFQASPDQSDLEVGQEVLDRIVLVHLGKDGSQDKFRMLLFMIRKLYSLVAGECSPDNPDATQHQEVLLGGFLYGMILKEKIDEYLQNIIAQVRMDINRGMAINFKDKRYMSRVLMRVNENIGSKMQYFLSTGNLVSQSGLDLQQVSGYTVVAEKINFYRFISHFRMVHRGSFFAQLKTTTVRKLLPESWGFLCPVHTPDGSPCGLLNHFAHKCRISTQQSDVSRIPSILYSLGVAPASHTFAAGPSLCCVQIDGKIIGWVSHEQGKIIADTLRYWKVEGKTPGLPIDLEIGYVPPSTRGQYPGLYLFGGHSRMLRPVRYLPLDKEDIVGPFEQVYMNIAVTPQEIQNNVHTHVEFTPTNILSILANLTPFSDFNQSPRNMYQCQMGKQTMGTPGVALCHRSDNKLYRLQTGQTPIVKANLYDDYGMDNFPNGFNAVVAVISYTGYDMDDAMIINKSADERGFGYGTMYKTEKVDLALNRNRGDPITQHFGFGNDEWPKEWLEKLDEDGLPYIGTYVEEGDPICAYFDDTLNKTKIKTYHSSEPAYIEEVNLIGDESNKFQELQTVSIKYRIRRTPQIGDKFSSRHGQKGVCSRKWPTIDMPFSETGIQPDIIINPHAFPSRMTIGMFVESLAGKAGALHGIAQDSTPWIFNEDDTPADYFGEQLAKAGYNYHGNEPMYSGATGEELRADIYVGVVYYQRLRHMVNDKFQVRSTGPVNSLTMQPVKGRKRHGGIRVGEMERDALIGHGTSFLLQDRLLNSSDYTQASVCRECGSILTTQQSVPRIGSISTVCCRRCSMRFEDAKKLLTKSEDGEKIFIDDSQIWEDGQGNKFVGGNETTTVAIPFVLKYLDSELSAMGIRLRYNVEPK

Entity 3 Entity 3 polymer

Description description	DNA-directed RNA polymerases I and III subunit RPAC1
Molecular weight formula_weight	37732.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

MSNIVGIEYNRVTNTTSTDFPGFSKDAENEWNVEKFKKDFEVNISSLDAREANFDLINIDTSIANAFRRIMISEVPSVAAEYVYFFNNTSVIQDEVLAHRIGLVPLKVDPDMLTWVDSNLPDDEKFTDENTIVLSLNVKCTRNPDAPKGSTDPKELYNNAHVYARDLKFEPQGRQSTTFADCPVVPADPDILLAKLRPGQEISLKAHCILGIGGDHAKFSPVSTASYRLLPQINILQPIKGESARRFQKCFPPGVIGIDEGSDEAYVKDARKDTVSREVLRYEEFADKVKLGRVRNHFIFNVESAGAMTPEEIFFKSVRILKNKAEYLKNCPITQ

Entity 4 Entity 4 polymer

Description description	DNA-directed RNA polymerase I subunit RPA14
Molecular weight formula_weight	14599.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

MMKGSRRTGNNTATTLNTPVVIHATQLPQHVSTDEVLQFLESFIDEKENIIDSTTMNTISGNAADADAAAVANTSLNIDTNLSSSISQLKRIQRDFKGLPPAQDFSAAPIQVSTTEKKETSIGVSATGGKKTTFADE

Entity 5 Entity 5 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC1
Molecular weight formula_weight	25117.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

MDQENERNISRLWRAFRTVKEMVKDRGYFITQEEVELPLEDFKAKYCDSMGRPQRKMMSFQANPTEESISKFPDMGSLWVEFCDEPSVGVKTMKTFVIHIQEKNFQTGIFVYQNNITPSAMKLVPSIPPATIETFNEAALVVNITHHELVPKHIRLSSDEKRELLKRYRLKESQLPRIQRADPVALYLGLKRGEVVKIIRKSETSGRYASYRICM

Entity 6 Entity 6 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC2
Molecular weight formula_weight	17931.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQHEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL

Entity 7 Entity 7 polymer

Description description	DNA-directed RNA polymerase I subunit RPA43
Molecular weight formula_weight	36264.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

MSQVKRANENRETARFIKKHKKQVTNPIDEKNGTSNCIVRVPIALYVSLAPMYLENPLQGVMKQHLNPLVMKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHVNLYVWQPQVGDVLEGYIFIQSASHIGLLIHDAFNASIKKNNIPVDWTFVHNDVEEDADVINTDENNGNNNNEDNKDSNGGSNSLGKFSFGNRSLGHWVDSNGEPIDGKLRFTVRNVHTTGRVVSVDGTLISDADEEGNGYNSSRSQAESLPIVSNKKIVFDDEVSIENKESHKELDLPEVKEDNGSEIVYEENTSESNDGESSDSD

Entity 8 Entity 8 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC3
Molecular weight formula_weight	16525.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

MSNTLFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIASSLNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSFGGLLMRLEGNYRNLNNLKQENAYLLIRR

Entity 9 Entity 9 polymer

Description description	DNA-directed RNA polymerase I subunit RPA12
Molecular weight formula_weight	13676.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

MSVVGSLIFCLDCGDLLENPNAVLGSNVECSQCKAIYPKSQFSNLKVVTTTADDAFPSSLRAKKSVVKTSLKKNELKDGATIKEKCPQCGNEEMNYHTLQLRSADEGATVFYTCTSCGYKFRTNN

Entity 10 Entity 10 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC5
Molecular weight formula_weight	8290.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKFLRYNPLEKRD

Entity 11 Entity 11 polymer

Description description	DNA-directed RNA polymerases I and III subunit RPAC2
Molecular weight formula_weight	16167.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

MTEDIEQKKTATEVTPQEPKHIQEEEEQDVDMTGDEEQEEEPDREKIKLLTQATSEDGTSASFQIVEEDHTLGNALRYVIMKNPDVEFCGYSIPHPSENLLNIRIQTYGETTAVDALQKGLKDLMDLCDVVESKFTEKIKSM

Entity 12 Entity 12 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC4
Molecular weight formula_weight	7730.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L

MSREGFQIPTNLDAAAAGTSQARTATLKYICAECSSKLSLSRTDAVRCKDCGHRILLKARTKRLVQFEAR

Entity 13 Entity 13 polymer

Description description	DNA-directed RNA polymerase I subunit RPA49
Molecular weight formula_weight	46721.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	M

MSVKRSVSEIEIESVQDQPSVAVGSFFKGFRAPSDTTFDLYKKKKSEKDEFVLHGENERLEYEGYTDSSSQASNQYVVGLFNPEKKSIQLYKAPVLVSKVVSKSSKNLRGPKIKSKSDTRPSALRNALGEAFGTKKAKKAIADLERNRIDSDKLTDSAIDIVDSVRTASKDLPTRAQLDEITSNDRPTPLANIDATDVEQIYPIESIIPKKELQFIRVSSILKEADKEKKLELFPYQNNSKYVAKKLDSLTQPSQMTKLQLLYYLSLLLGVYENRRVNNKTKLLERLNSPPEILVDGILSRFTVIKPGQFGRSKDRSYFIDPQNEDKILCYILAIIMHLDNFIVEITPLAHELNLKPSKVVSLFRVLGAIVKGATVAQAEAFGIPKSTAASYKIATMKVPFKLPEMTRRGRGPRR

Entity 14 Entity 14 polymer

Description description	DNA-directed RNA polymerase I subunit RPA34
Molecular weight formula_weight	26933.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	N

MSKLSKDYVSDSDSDDEVISNEFSIPDGFKKCKHLKNFPLNGDNKKKAKQQQVWLIKFPSNVDISKLKSLPVDFESSTTMTIDKHDYKIMDDTDIESSLTQDNLSNMTLLVPSESKESLKIASTAKDNAPLQFDKVFSVSETAKIPAIDYSKVRVPRKDVPKVEGLKLEHFATGYDAEDFHVAEEVKENKKEPKKRSHHDDEEESSEKKKKKKEKREKREKKDKKDKKKKHRD

Entity 15 Entity 15 polymer

Description description	RNA polymerase I-specific transcription initiation factor RRN3
Molecular weight formula_weight	72458.3 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	O

MMAFENTSKRPPQDFVAPIDQKKRKVQFSDSTGLVTLQPEEIKDEVFSAAMYSRFVKSALDDLDKNDSTQIGIIANQVALPSKNPERINDKNLNILLDILSSNINRIESSRGTFLIQSIINFEKWWELPPHTLSKYIYFIKILCSSIPKWWQDVSMILVSCFILPIKQTVCHHDMLKYFLRMIPSSMGFIDTYLAKFFPNKNDTRRKLVNYTSNLLKLRGYCSELGFQIWSLLIEKIISIDVELQNELDELDDDVDDDDLEEVDLEDDDDLDDDSGDDDDENCGNSNEELRSGAADGSQSDSEDMDIIEGMDGTEEYNVELTQGIKELSTKLDSILTLVSTHVEEQVTPESLESGEGVGVFNTLTTLFKTHVLPTYYTRSIQYIMFHVSQQQLELMDSFLVTLIDISFAVNEAAEKKIKSLQYLGSYIARAKKLSRTQIIFVASYLTSWLNRYVIEREEEVDQRGGMERFKHFYAAFQALCYIFCFRHNIFRDTDGNWECELDKFFQRMVISKFNPLKFCNENVMLMFARIAQQESVAYCFSIIENNNNERLRGIIGKADSDKKENSAQANTTSSSWSLATRQQFIDLQSYFPYDPLFLKNYKILMKEYYIEWSEASGEYESDGSDD

Source Source

Source organism organism	Saccharomyces cerevisiae
Common name common_name	;Baker's yeast ;
NCBI taxonomy ID ncbi_taxonomy_id	4932
Host organism host_organism	Escherichia coli BL21(DE3)

Entity 16 Entity 16 polymer

Description description	RNA polymerase I-specific transcription initiation factor RRN6
Molecular weight formula_weight	102163.2 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	P

MSEGQIPSSDVLGSQLGVGVQGASLYCPQENYTTKKQEKPQWLRPVDDTLAEDALDLHIVVKSLLCDTAIRYISDDKVLQESDADDDLITSDIDEDTDNQGDTSIVVNPVIPVVPKDVHFFKKVDVGNDSMFGVNCDTPVSFQDYIPSDLLRNLDDTLQESTNSSRPMQDAFFWDPTVANRLDSQYIQTASDLRNYRDGTEIIAYASGKTGSVLNIAVLTRQNTLHLNRHNNVTSIELHSPIKSIKIPGASESIGRRSNLVGIITENSFQIFRIESVHSRSCDVMVSSSEPLYFVEIDDLQVVDFAFNPWDLQQFAIIDIKGNWSIGRIPKNFNNNNKRKLQLIDNLHGTIFDPEELSSWKRIEWFSHFQKILVFDRSKMIEIDFMNNWQTEVVQAKAWSNIRDYKRIDDKNGILLTSREIIIVGASESNDPVRRISWKHDLDPDDTTLRITVQKVKKPDHILLVAFVYSMRHKRIYMHVFSHRKANLFQSLGCSTVLEIPGGTPTGIETILTLDHIDDESRREEDADENFELVVDFLVKLRNSSEVYYYALSNTQNSEPNKQETPIIVDHPEWASLFNNADEREKESIGALVSQIKLKERERISRVQNLIEHENSHDEDKYLQDLGYRLSIATNELLESWQKTKDESILSGSLSHSKLKNLLENSDSFASIPEFSSLLDQFFQYYQDQDVTFIGFEKLLHLFLHEDVPGLDIFYNKLLQCWVLVSPQAELLTKEIVKDIIWSLARLEKPSLFEPIQNEISRSLSGPYQDIISSWDMDDINEEDESNEFNFDSQFSAPFNGRPPFNLNSQSQIPTIKSSQSSGLARRKRILKTQSQKATPLSQSTQNLSVLPDSMTPAFTLMQPPSSQISFVNDSQPRNSQKAKKKKKRIRGFG

Source Source

Source organism organism	Saccharomyces cerevisiae
Common name common_name	;Baker's yeast ;
NCBI taxonomy ID ncbi_taxonomy_id	4932
Host organism host_organism	Escherichia coli BL21(DE3)

Entity 17 Entity 17 polymer

Description description	RNA polymerase I-specific transcription initiation factor RRN7
Molecular weight formula_weight	60435.2 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q

MSTFIRGPICGTDNCPSRLWRIIDGRRTCQYGHVMEGDVEFNDDEDDLNGLGAGVITRRLNLTTNATGSFQSSQLTNSQLLQQQQRQSHKKFKKLIGHEAKLLFLKSFQFILKRQIRWLITEMRFPKEFEHVAKIIWLKILKTINDQPQEELKLQLHMTSTISILYLASTHLSLPVYTCDYIKWICTAKMPYFQASEILPKSWRIQLPNYYVSILEGSISPFNGQLYNKIALTCGMIHFKEFFNSEISCQGLLLKLVMQCALPPEFYFYTKQVIEFEETDIRNLTLWERTDERHTGRVSNHAELRVLSYFMLTINWMLSFDRDRQYPLKWILSLTESLTQRTTTSESIGRNIVKVVYPDKPTSSDYFQWSEEETLEFLKWMEKQFLPTQTKSLHNENGSMEMTIDQKIARRKLYKIFPLDREANHDGEFNDSTHQLTFIEDLQERYAKQTPFFESNKIRDSLNYQEANPPARKEAIGRLLTHIASQLLVDFAISKEQLKDCISRIKNACLHRMN

Source Source

Source organism organism	Saccharomyces cerevisiae
Common name common_name	;Baker's yeast ;
NCBI taxonomy ID ncbi_taxonomy_id	4932
Host organism host_organism	Escherichia coli BL21(DE3)

Entity 18 Entity 18 polymer

Description description	RNA polymerase I-specific transcription initiation factor RRN11
Molecular weight formula_weight	59334.3 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	R

MFEVPITLTNRKFAQRRKLKYQYINYISRRFDRISKKSTTTDSLPTPENSAAENNDEEEGQNSEAGTYRRSVLQQKKRRRERHWRSVVGEIYSTTESETDSQEEETEEGGEHDTGIDKEDSDEERKFWKKYEKPEKSFEIWRTVSSQNKQPINKQKMTYHNFKKIEKIPLRKMEIPLLHCTKENKLYFQSISRGLEPLKTSTSEVRNYRTRHIVTLTDLLHLNVSRHNWSLAYKIFATLIRIPGVQIKSLWGIGVEILDNLSNSSSGLDFLQWMCQIYSSKSRFVQNINYRSIVPPFQTGSRTHTAKFAITYLWSSLINCQKSMEPSSNIIDKPFDTENDLLQELIDKISEWVLTPPFMEDAEVWFIYASCHLLKADTLSRQFVNDNKNNDLIGLDRDIKINQVIKHIHYVRTFLKICLDKGGFAVPSRLIENQLKSFESRLYGEAQDIQERDVANVYDSIDNSSVENSFGDVYETNAEFLDTQLMDLSPEDNGLDEMHYSDEDSSE

Source Source

Source organism organism	Saccharomyces cerevisiae
Common name common_name	;Baker's yeast ;
NCBI taxonomy ID ncbi_taxonomy_id	4932
Host organism host_organism	Escherichia coli BL21(DE3)

Entity 19 Entity 19 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	man
Copy count entity_count	8

▶

Resolution resolution	7.70 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	8317

Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation II)

1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-directed RNA polymerase I subunit RPA190

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase I subunit RPA135

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I and III subunit RPAC1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase I subunit RPA14

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase I subunit RPA43

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase I subunit RPA12

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC5

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer