5fj8

Cryo-EM structure of yeast RNA polymerase III elongation complex at 3. 9 A

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2 × 1 （P22276） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 × 1 （P07703） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9 × 1 （P47076） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 × 1 （P20434） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2 × 1 （P20435） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8 × 1 （P35718） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 × 1 （P20436） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10 × 1 （Q04307） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 × 1 （P22139） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 × 1 （P28000） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 × 1 （P40422） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5 × 1 （P36121） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4 × 1 （P25441） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3 × 1 （P32349） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6 × 1 （P32910） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7 × 1 （P17890） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPC1_YEAST
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–1460； UniProt 1–1460

DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2

OrganismNot specified

UniProt P22276

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1 × 1 （P04051） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 × 1 （P07703） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9 × 1 （P47076） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 × 1 （P20434） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2 × 1 （P20435） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8 × 1 （P35718） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 × 1 （P20436） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10 × 1 （Q04307） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 × 1 （P22139） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 × 1 （P28000） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 × 1 （P40422） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5 × 1 （P36121） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4 × 1 （P25441） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3 × 1 （P32349） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6 × 1 （P32910） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7 × 1 （P17890） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPC2_YEAST
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–1149； UniProt 1–1149

DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1

OrganismNot specified

UniProt P07703

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1 × 1 （P04051） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2 × 1 （P22276） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9 × 1 （P47076） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 × 1 （P20434） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2 × 1 （P20435） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8 × 1 （P35718） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 × 1 （P20436） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10 × 1 （Q04307） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 × 1 （P22139） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 × 1 （P28000） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 × 1 （P40422） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5 × 1 （P36121） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4 × 1 （P25441） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3 × 1 （P32349） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6 × 1 （P32910） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7 × 1 （P17890） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAC1_YEAST
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–335； UniProt 1–335

DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9

OrganismNot specified

UniProt P47076

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1 × 1 （P04051） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2 × 1 （P22276） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 × 1 （P07703） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 × 1 （P20434） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2 × 1 （P20435） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8 × 1 （P35718） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 × 1 （P20436） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10 × 1 （Q04307） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 × 1 （P22139） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 × 1 （P28000） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 × 1 （P40422） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5 × 1 （P36121） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4 × 1 （P25441） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3 × 1 （P32349） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6 × 1 （P32910） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7 × 1 （P17890） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPC9_YEAST
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain D； PDBConstruct 1–161； UniProt 1–161

DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1

OrganismNot specified

UniProt P20434

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1 × 1 （P04051） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2 × 1 （P22276） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 × 1 （P07703） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9 × 1 （P47076） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2 × 1 （P20435） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8 × 1 （P35718） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 × 1 （P20436） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10 × 1 （Q04307） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 × 1 （P22139） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 × 1 （P28000） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 × 1 （P40422） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5 × 1 （P36121） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4 × 1 （P25441） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3 × 1 （P32349） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6 × 1 （P32910） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7 × 1 （P17890） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB1_YEAST
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain E； PDBConstruct 1–215； UniProt 1–215

DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2

OrganismNot specified

UniProt P20435

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1 × 1 （P04051） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2 × 1 （P22276） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 × 1 （P07703） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9 × 1 （P47076） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 × 1 （P20434） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8 × 1 （P35718） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 × 1 （P20436） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10 × 1 （Q04307） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 × 1 （P22139） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 × 1 （P28000） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 × 1 （P40422） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5 × 1 （P36121） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4 × 1 （P25441） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3 × 1 （P32349） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6 × 1 （P32910） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7 × 1 （P17890） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB2_YEAST
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain F； PDBConstruct 1–155； UniProt 1–155

DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8

OrganismNot specified

UniProt P35718

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1 × 1 （P04051） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2 × 1 （P22276） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 × 1 （P07703） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9 × 1 （P47076） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 × 1 （P20434） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2 × 1 （P20435） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 × 1 （P20436） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10 × 1 （Q04307） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 × 1 （P22139） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 × 1 （P28000） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 × 1 （P40422） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5 × 1 （P36121） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4 × 1 （P25441） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3 × 1 （P32349） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6 × 1 （P32910） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7 × 1 （P17890） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPC8_YEAST
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain G； PDBConstruct 1–212； UniProt 1–212

DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3

OrganismNot specified

UniProt P20436

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1 × 1 （P04051） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2 × 1 （P22276） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 × 1 （P07703） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9 × 1 （P47076） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 × 1 （P20434） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2 × 1 （P20435） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8 × 1 （P35718） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10 × 1 （Q04307） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 × 1 （P22139） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 × 1 （P28000） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 × 1 （P40422） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5 × 1 （P36121） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4 × 1 （P25441） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3 × 1 （P32349） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6 × 1 （P32910） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7 × 1 （P17890） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB3_YEAST
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain H； PDBConstruct 1–146； UniProt 1–146

DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10

OrganismNot specified

UniProt Q04307

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1 × 1 （P04051） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2 × 1 （P22276） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 × 1 （P07703） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9 × 1 （P47076） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 × 1 （P20434） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2 × 1 （P20435） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8 × 1 （P35718） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 × 1 （P20436） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 × 1 （P22139） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 × 1 （P28000） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 × 1 （P40422） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5 × 1 （P36121） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4 × 1 （P25441） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3 × 1 （P32349） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6 × 1 （P32910） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7 × 1 （P17890） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPC10_YEAST
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain I； PDBConstruct 1–110； UniProt 1–110

DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5

OrganismNot specified

UniProt P22139

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1 × 1 （P04051） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2 × 1 （P22276） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 × 1 （P07703） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9 × 1 （P47076） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 × 1 （P20434） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2 × 1 （P20435） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8 × 1 （P35718） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 × 1 （P20436） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10 × 1 （Q04307） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 × 1 （P28000） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 × 1 （P40422） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5 × 1 （P36121） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4 × 1 （P25441） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3 × 1 （P32349） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6 × 1 （P32910） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7 × 1 （P17890） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB5_YEAST
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain J； PDBConstruct 1–70； UniProt 1–70

DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2

OrganismNot specified

UniProt P28000

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1 × 1 （P04051） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2 × 1 （P22276） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 × 1 （P07703） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9 × 1 （P47076） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 × 1 （P20434） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2 × 1 （P20435） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8 × 1 （P35718） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 × 1 （P20436） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10 × 1 （Q04307） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 × 1 （P22139） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 × 1 （P40422） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5 × 1 （P36121） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4 × 1 （P25441） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3 × 1 （P32349） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6 × 1 （P32910） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7 × 1 （P17890） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAC2_YEAST
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain K； PDBConstruct 1–142； UniProt 1–142

DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4

OrganismNot specified

UniProt P40422

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1 × 1 （P04051） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2 × 1 （P22276） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 × 1 （P07703） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9 × 1 （P47076） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 × 1 （P20434） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2 × 1 （P20435） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8 × 1 （P35718） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 × 1 （P20436） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10 × 1 （Q04307） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 × 1 （P22139） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 × 1 （P28000） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5 × 1 （P36121） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4 × 1 （P25441） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3 × 1 （P32349） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6 × 1 （P32910） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7 × 1 （P17890） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB4_YEAST
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain L； PDBConstruct 1–70； UniProt 1–70

DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5

OrganismNot specified

UniProt P36121

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1 × 1 （P04051） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2 × 1 （P22276） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 × 1 （P07703） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9 × 1 （P47076） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 × 1 （P20434） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2 × 1 （P20435） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8 × 1 （P35718） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 × 1 （P20436） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10 × 1 （Q04307） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 × 1 （P22139） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 × 1 （P28000） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 × 1 （P40422） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4 × 1 （P25441） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3 × 1 （P32349） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6 × 1 （P32910） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7 × 1 （P17890） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPC5_YEAST
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain M； PDBConstruct 1–282； UniProt 1–282

DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4

OrganismNot specified

UniProt P25441

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1 × 1 （P04051） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2 × 1 （P22276） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 × 1 （P07703） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9 × 1 （P47076） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 × 1 （P20434） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2 × 1 （P20435） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8 × 1 （P35718） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 × 1 （P20436） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10 × 1 （Q04307） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 × 1 （P22139） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 × 1 （P28000） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 × 1 （P40422） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5 × 1 （P36121） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3 × 1 （P32349） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6 × 1 （P32910） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7 × 1 （P17890） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPC4_YEAST
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain N； PDBConstruct 1–422； UniProt 1–422

DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3

OrganismNot specified

UniProt P32349

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1 × 1 （P04051） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2 × 1 （P22276） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 × 1 （P07703） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9 × 1 （P47076） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 × 1 （P20434） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2 × 1 （P20435） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8 × 1 （P35718） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 × 1 （P20436） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10 × 1 （Q04307） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 × 1 （P22139） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 × 1 （P28000） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 × 1 （P40422） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5 × 1 （P36121） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4 × 1 （P25441） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6 × 1 （P32910） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7 × 1 （P17890） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPC3_YEAST
Isoform	—
PDB entities	15
Chains and sequence ranges	Author chain O； PDBConstruct 1–654； UniProt 1–654

DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6

OrganismNot specified

UniProt P32910

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1 × 1 （P04051） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2 × 1 （P22276） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 × 1 （P07703） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9 × 1 （P47076） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 × 1 （P20434） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2 × 1 （P20435） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8 × 1 （P35718） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 × 1 （P20436） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10 × 1 （Q04307） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 × 1 （P22139） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 × 1 （P28000） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 × 1 （P40422） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5 × 1 （P36121） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4 × 1 （P25441） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3 × 1 （P32349） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7 × 1 （P17890） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPC6_YEAST
Isoform	—
PDB entities	16
Chains and sequence ranges	Author chain P； PDBConstruct 1–317； UniProt 1–317

DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7

OrganismNot specified

UniProt P17890

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 17 DNA 2 RNA 1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1 × 1 （P04051） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2 × 1 （P22276） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1 × 1 （P07703） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9 × 1 （P47076） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1 × 1 （P20434） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2 × 1 （P20435） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8 × 1 （P35718） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3 × 1 （P20436） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10 × 1 （Q04307） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5 × 1 （P22139） DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2 × 1 （P28000） DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4 × 1 （P40422） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5 × 1 （P36121） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4 × 1 （P25441） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3 × 1 （P32349） DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6 × 1 （P32910） RNA × 1 NON-TEMPLATE DNA × 1 TEMPLATE DNA × 1 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPC7_YEAST
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain Q； PDBConstruct 1–69； UniProt 1–69

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	5fj8
Deposition date deposition_date	2015-10-06
Structure title title	Cryo-EM structure of yeast RNA polymerase III elongation complex at 3. 9 A
Keywords keywords	RNA POLYMERASE III, POL III, TRANSCRIPTION, RNA POLYMERASE; TRANSCRIPTION
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

5fj8__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

5fj8__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

5fj8__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	58.20 Å
Rg (electron density)	58.37 Å
Total Rg	58.37 Å
Atom count	39284
Residues	4886
Excluded volume	699770 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`5fj8__assembly_1__model_1`	eicosameric （20）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (21)

Entity 1 Entity 1 polymer

Description description	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1
Molecular weight formula_weight	162517.8 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	39284

MKEVVVSETPKRIKGLEFSALSAADIVAQSEVEVSTRDLFDLEKDRAPKANGALDPKMGVSSSSLECATCHGNLASCHGHFGHLKLALPVFHIGYFKATIQILQGICKNCSAILLSETDKRQFLHELRRPGVDNLRRMGILKKILDQCKKQRRCLHCGALNGVVKKAAAGAGSAALKIIHDTFRWVGKKSAPEKDIWVGEWKEVLAHNPELERYVKRCMDDLNPLKTLNLFKQIKSADCELLGIDATVPSGRPETYIWRYLPAPPVCIRPSVMMQDSPASNEDDLTVKLTEIVWTSSLIKAGLDKGISINNMMEHWDYLQLTVAMYINSDSVNPAMLPGSSNGGGKVKPIRGFCQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLSIDEVAVPDRVAKVLTYPEKVTRYNRHKLQELIVNGPNVHPGANYLLKRNEDARRNLRYGDRMKLAKNLQIGDVVERHLEDGDVVLFNRQPSLHRLSILSHYAKIRPWRTFRLNECVCTPYNADFDGDEMNLHVPQTEEARAEAINLMGVKNNLLTPKSGEPIIAATQDFITGSYLISHKDSFYDRATLTQLLSMMSDGIEHFDIPPPAIMKPYYLWTGKQVFSLLIKPNHNSPVVINLDAKNKVFVPPKSKSLPNEMSQNDGFVIIRGSQILSGVMDKSVLGDGKKHSVFYTILRDYGPQEAANAMNRMAKLCARFLGNRGFSIGINDVTPADDLKQKKEELVEIAYHKCDELITLFNKGELETQPGCNEEQTLEAKIGGLLSKVREEVGDVCINELDNWNAPLIMATCGSKGSTLNVSQMVAVVGQQIISGNRVPDGFQDRSLPHFPKNSKTPQSKGFVRNSFFSGLSPPEFLFHAISGREGLVDTAVKTAETGYMSRRLMKSLEDLSCQYDNTVRTSANGIVQFTYGGDGLDPLEMEGNAQPVNFNRSWDHAYNITFNNQDKGLLPYAIMETANEILGPLEERLVRYDNSGCLVKREDLNKAEYVDQYDAERDFYHSLREYINGKATALANLRKSRGMLGLLEPPAKELQGIDPDETVPDNVKTSVSQLYRISEKSVRKFLEIALFKYRKARLEPGTAIGAIGAQSIGEPGTQMTLKTFHFAGVASMNVTLGVPRIKEIINASKVISTPIINAVLVNDNDERAARVVKGRVEKTLLSDVAFYVQDVYKDNLSFIQVRIDLGTIDKLQLELTIEDIAVAITRASKLKIQASDVNIIGKDRIAINVFPEGYKAKSISTSAKEPSENDVFYRMQQLRRALPDVVVKGLPDISRAVINIRDDGKRELLVEGYGLRDVMCTDGVIGSRTTTNHVLEVFSVLGIEAARYSIIREINYTMSNHGMSVDPRHIQLLGDVMTYKGEVLGITRFGLSKMRDSVLQLASFEKTTDHLFDAAFYMKKDAVEGVSECIILGQTMSIGTGSFKVVKGTNISEKDLVPKRCLFESLSNEAALKAN

Entity 2 Entity 2 polymer

Description description	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2
Molecular weight formula_weight	129629.4 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

MVAATKRRKTHIHKHVKDEAFDDLLKPVYKGKKLTDEINTAQDKWHLLPAFLKVKGLVKQHLDSFNYFVDTDLKKIIKANQLILSDVDPEFYLKYVDIRVGKKSSSSTKDYLTPPHECRLRDMTYSAPIYVDIEYTRGRNIIMHKDVEIGRMPIMLRSNKCILYDADESKMAKLNECPLDPGGYFIVNGTEKVILVQEQLSKNRIIVEADEKKGIVQASVTSSTHERKSKTYVITKNGKIYLKHNSIAEEIPIAIVLKACGILSDLEIMQLVCGNDSSYQDIFAVNLEESSKLDIYTQQQALEYIGAKVKTMRRQKLTILQEGIEAIATTVIAHLTVEALDFREKALYIAMMTRRVVMAMYNPKMIDDRDYVGNKRLELAGQLISLLFEDLFKKFNNDFKLSIDKVLKKPNRAMEYDALLSINVHSNNITSGLNRAISTGNWSLKRFKMERAGVTHVLSRLSYISALGMMTRISSQFEKSRKVSGPRALQPSQFGMLCTADTPEGEACGLVKNLALMTHITTDDEEEPIKKLCYVLGVEDITLIDSASLHLNYGVYLNGTLIGSIRFPTKFVTQFRHLRRTGKVSEFISIYSNSHQMAVHIATDGGRICRPLIIVSDGQSRVKDIHLRKLLDGELDFDDFLKLGLVEYLDVNEENDSYIALYEKDIVPSMTHLEIEPFTILGAVAGLIPYPHHNQSPRNTYQCAMGKQAIGAIAYNQFKRIDTLLYLMTYPQQPMVKTKTIELIDYDKLPAGQNATVAVMSYSGYDIEDALVLNKSSIDRGFGRCETRRKTTTVLKRYANHTQDIIGGMRVDENGDPIWQHQSLGPDGLGEVGMKVQSGQIYINKSVPTNSADAPNPNNVNVQTQYREAPVIYRGPEPSHIDQVMMSVSDNDQALIKVLLRQNRRPELGDKFSSRHGQKGVCGIIVKQEDMPFNDQGIVPDIIMNPHGFPSRMTVGKMIELISGKAGVLNGTLEYGTCFGGSKLEDMSKILVDQGFNYSGKDMLYSGITGECLQAYIFFGPIYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCVIAYGASQLLLERLMISSDAFEVDVCDKCGLMGYSGWCTTCKSAENIIKMTIPYAAKLLFQELLSMNIAPRLRLEDIFQQ

Entity 3 Entity 3 polymer

Description description	DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1
Molecular weight formula_weight	37732.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

MSNIVGIEYNRVTNTTSTDFPGFSKDAENEWNVEKFKKDFEVNISSLDAREANFDLINIDTSIANAFRRIMISEVPSVAAEYVYFFNNTSVIQDEVLAHRIGLVPLKVDPDMLTWVDSNLPDDEKFTDENTIVLSLNVKCTRNPDAPKGSTDPKELYNNAHVYARDLKFEPQGRQSTTFADCPVVPADPDILLAKLRPGQEISLKAHCILGIGGDHAKFSPVSTASYRLLPQINILQPIKGESARRFQKCFPPGVIGIDEGSDEAYVKDARKDTVSREVLRYEEFADKVKLGRVRNHFIFNVESAGAMTPEEIFFKSVRILKNKAEYLKNCPITQ

Entity 4 Entity 4 polymer

Description description	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9
Molecular weight formula_weight	18623.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITRNVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEKLQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIISGYA

Entity 5 Entity 5 polymer

Description description	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1
Molecular weight formula_weight	25117.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

MDQENERNISRLWRAFRTVKEMVKDRGYFITQEEVELPLEDFKAKYCDSMGRPQRKMMSFQANPTEESISKFPDMGSLWVEFCDEPSVGVKTMKTFVIHIQEKNFQTGIFVYQNNITPSAMKLVPSIPPATIETFNEAALVVNITHHELVPKHIRLSSDEKRELLKRYRLKESQLPRIQRADPVALYLGLKRGEVVKIIRKSETSGRYASYRICM

Entity 6 Entity 6 polymer

Description description	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2
Molecular weight formula_weight	17931.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQHEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL

Entity 7 Entity 7 polymer

Description description	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8
Molecular weight formula_weight	24349.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

MFILSKIADLVRIPPDQFHRDTISAITHQLNNKFANKIIPNVGLCITIYDLLTVEEGQLKPGDGSSYINVTFRAVVFKPFLGEIVTGWISKCTAEGIKVSLLGIFDDIFIPQNMLFEGCYYTPEESAWIWPMDEETKLYFDVNEKIRFRIEREVFVDVKPKSPKERELEERAQLENEIEGKNEETPQNEKPPAYALLGSCQTDGMGLVSWWE

Entity 8 Entity 8 polymer

Description description	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3
Molecular weight formula_weight	16525.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

MSNTLFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIASSLNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSFGGLLMRLEGNYRNLNNLKQENAYLLIRR

Entity 9 Entity 9 polymer

Description description	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10
Molecular weight formula_weight	12525.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

MLSFCPSCNNMLLITSGDSGVYTLACRSCPYEFPIEGIEIYDRKKLPRKEVDDVLGGGWDNVDQTKTQCPNYDTCGGESAYFFQLQIRSADEPMTTFYKCVNCGHRWKEN

Entity 10 Entity 10 polymer

Description description	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5
Molecular weight formula_weight	8290.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKFLRYNPLEKRD

Entity 11 Entity 11 polymer

Description description	DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2
Molecular weight formula_weight	16167.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

MTEDIEQKKTATEVTPQEPKHIQEEEEQDVDMTGDEEQEEEPDREKIKLLTQATSEDGTSASFQIVEEDHTLGNALRYVIMKNPDVEFCGYSIPHPSENLLNIRIQTYGETTAVDALQKGLKDLMDLCDVVESKFTEKIKSM

Entity 12 Entity 12 polymer

Description description	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4
Molecular weight formula_weight	7730.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L

MSREGFQIPTNLDAAAAGTSQARTATLKYICAECSSKLSLSRTDAVRCKDCGHRILLKARTKRLVQFEAR

Entity 13 Entity 13 polymer

Description description	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5
Molecular weight formula_weight	32178.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	M

MSIDNKLFVTEEDEEDRTQDRADVEDESNDIDMIADENGTNSAIANEQEEKSEEVKAEDDTGEEEEDDPVIEEFPLKISGEEESLHVFQYANRPRLVGRKPAEHPFISAARYKPKSHLWEIDIPLDEQAFYNKDKAESEWNGVNVQTLKGVGVENNGQYAAFVKDMQVYLVPIERVAQLKPFFKYIDDANVTRKQEDARRNPNPSSQRAQVVTMSVKSVNDPSQNRLTGSLLAHKVADEEANIELTWAEGTFEQFKDTIVKEAEDKTLVALEKQEDYIDNLV

Entity 14 Entity 14 polymer

Description description	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4
Molecular weight formula_weight	46751.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	N

MSSNKGNGRLPSLKDSSSNGGGSAKPSLKFKPKAVARKSKEEREAAASKVKLEEESKRGNDKKHFNNKNKRVTGAGGQQRRMAKYLNNTHVISSGPLAAGNFVSEKGDLRRGFIKSEGSGSSLVQKGLETIDNGAESSENEAEDDDNEGVASKSKKKFNMGKEFEARNLIEDEDDGESEKSSDVDMDDEEWRSKRIEQLFPVRPVRVRHEDVETVKREIQEALSEKPTREPTPSVKTEPVGTGLQSYLEERERQVNEKLADLGLEKEFQSVDGKEAAAELELLNADHQHILRKLKKMNNKPERFMVFQLPTRLPAFERPAVKEEKEDMETQASDPSKKKKNIKKKDTKDALSTRELAGKVGSIRVHKSGKLSVKIGNVVMDIGKGAETTFLQDVIALSIADDASSAELLGRVDGKIVVTPQI

Entity 15 Entity 15 polymer

Description description	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3
Molecular weight formula_weight	74112.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	O

MDELLGEALSAENQTGESTVESEKLVTPEDVMTISSLEQRTLNPDLFLYKELVKAHLGERAASVIGMLVALGRLSVRELVEKIDGMDVDSVKTTLVSLTQLRCVKYLQETAISGKKTTYYYYNEEGIHILLYSGLIIDEIITQMRVNDEEEHKQLVAEIVQNVISLGSLTVEDYLSSVTSDSMKYTISSLFVQLCEMGYLIQISKLHYTPIEDLWQFLYEKHYKNIPRNSPLSDLKKRSQAKMNAKTDFAKIINKPNELSQILTVDPKTSLRIVKPTVSLTINLDRFMKGRRSKQLINLAKTRVGSVTAQVYKIALRLTEQKSPKIRDPLTQTGLLQDLEEAKSFQDEAELVEEKTPGLTFNAIDLARHLPAELDLRGSLLSRKPSDNKKRSGSNAAASLPSKKLKTEDGFVIPALPAAVSKSLQESGDTQEEDEEEEDLDADTEDPHSASLINSHLKILASSNFPFLNETKPGVYYVPYSKLMPVLKSSVYEYVIASTLGPSAMRLSRCIRDNKLVSEKIINSTALMKEKDIRSTLASLIRYNSVEIQEVPRTADRSASRAVFLFRCKETHSYNFMRQNLEWNMANLLFKKEKLKQENSTLLKKANRDDVKGRENELLLPSELNQLKMVNERELNVFARLSRLLSLWEVFQMA

Entity 16 Entity 16 polymer

Description description	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC6
Molecular weight formula_weight	36174.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	P

MSGMIENGLQLSDNAKTLHSQMMSKGIGALFTQQELQKQMGIGSLTDLMSIVQELLDKNLIKLVKQNDELKFQGVLESEAQKKATMSAEEALVYSYIEASGREGIWSKTIKARTNLHQHVVLKCLKSLESQRYVKSVKSVKFPTRKIYMLYSLQPSVDITGGPWFTDGELDIEFINSLLTIVWRFISENTFPNGFKNFENGPKKNVFYAPNVKNYSTTQEILEFITAAQVANVELTPSNIRSLCEVLVYDDKLEKVTHDCYRVTLESILQMNQGEGEPEAGNKALEDEEEFSIFNYFKMFPASKHDKEVVYFDEWTI

Entity 17 Entity 17 polymer

Description description	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7
Molecular weight formula_weight	10349.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q

MSSYRGGSRGGGSNYMSNLPFGLGYGDVGKNHITEFPSIPLPINGPITNKERSLAVKYINFGKTVKDGPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Entity 18 Entity 18 polymer

Description description	RNA
Molecular weight formula_weight	7040.6 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	R

AAGTCAAGTACTTACGCCTGGTC

Entity 19 Entity 19 polymer

Description description	NON-TEMPLATE DNA
Molecular weight formula_weight	4608.0 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	S

GAAAGTACTTGACTT

Entity 20 Entity 20 polymer

Description description	TEMPLATE DNA
Molecular weight formula_weight	2893.8 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	T

AGACCAGGC

Entity 21 Entity 21 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	6

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6. Fold Classification (SCOP + CATH) 14 domains

CATH v4.4 (14 domains)

Domain ID domain_id	5fj8A03
Class class	2 — Mainly Beta
Architecture architecture	40 — Beta Barrel
Topology topology	40 — Barwin-like endoglucanases
Homologous superfamily homologous superfamily	20 —

Domain ID domain_id	5fj8A04
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1490 — Dna Ligase; domain 1
Homologous superfamily homologous superfamily	180 — RNA polymerase ii

Domain ID domain_id	5fj8A06
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	132 — Topoisomerase I; Chain A, domain 4
Homologous superfamily homologous superfamily	30 — RNA polymerase Rpb1 funnel domain

Domain ID domain_id	5fj8C01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1360 — Gyrase A; domain 2
Homologous superfamily homologous superfamily	10 — RNA polymerase, RBP11-like subunit

Domain ID domain_id	5fj8C02
Class class	2 — Mainly Beta
Architecture architecture	170 — Beta Complex
Topology topology	120 — RNA Polymerase Alpha Subunit; Chain A, domain 2
Homologous superfamily homologous superfamily	12 — DNA-directed RNA polymerase, insert domain

Domain ID domain_id	5fj8E01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	1340 — Dna-directed Rna Polymerases I, Ii, And Iii 27 Kd Polypeptide; Chain: A; domain 1
Homologous superfamily homologous superfamily	10 — RNA polymerase, Rpb5, N-terminal domain

Domain ID domain_id	5fj8E02
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	940 — Eukaryotic RPB6 RNA polymerase subunit
Homologous superfamily homologous superfamily	20 — RPB5-like RNA polymerase subunit

Domain ID domain_id	5fj8F00
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	940 — Eukaryotic RPB6 RNA polymerase subunit
Homologous superfamily homologous superfamily	10 — RNA polymerase subunit, RPB6/omega

Domain ID domain_id	5fj8G01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1490 — Dna Ligase; domain 1
Homologous superfamily homologous superfamily	120 — RNA polymerase Rpb7-like, N-terminal domain

Domain ID domain_id	5fj8G02
Class class	2 — Mainly Beta
Architecture architecture	40 — Beta Barrel
Topology topology	50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Homologous superfamily homologous superfamily	140 — Nucleic acid-binding proteins

Domain ID domain_id	5fj8H00
Class class	2 — Mainly Beta
Architecture architecture	40 — Beta Barrel
Topology topology	50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Homologous superfamily homologous superfamily	140 — Nucleic acid-binding proteins

Domain ID domain_id	5fj8J00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	10 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily	60 — Homeodomain-like

Domain ID domain_id	5fj8K00
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1360 — Gyrase A; domain 2
Homologous superfamily homologous superfamily	10 — RNA polymerase, RBP11-like subunit

Domain ID domain_id	5fj8L00
Class class	2 — Mainly Beta
Architecture architecture	20 — Single Sheet
Topology topology	28 — Rubrerythrin, domain 2
Homologous superfamily homologous superfamily	30 — RNA polymerase ii, chain L

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	1

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	3.900 Å
Low-resolution limit resolution_low	— Å
R factor r_factor	—
R_work r_work	—
R_free r_free	—

Resolution resolution	3.90 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	49543

Cryo-EM structure of yeast RNA polymerase III elongation complex at 3. 9 A

1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer