1i50

RNA POLYMERASE II CRYSTAL FORM II AT 2.8 A RESOLUTION

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 10	DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 1 （P08518） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 1 （P16370） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 1 （P20434） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 1 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 1 （P20436） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 1 （P27999） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 1 （P22139） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 1 （P38902） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 1 （P40422） ZINC ION × 8 MANGANESE (II) ION × 1 water × 7	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 20	DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 2 （P08518） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 2 （P16370） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 2 （P20434） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 2 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 2 （P20436） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 2 （P27999） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 2 （P22139） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 2 （P38902） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 2 （P40422） ZINC ION × 16 MANGANESE (II) ION × 2 water × 14	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB1_YEAST
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–1733； UniProt 1–1733

DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE

OrganismNot specified

UniProt P08518

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 10	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 1 （P04050） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 1 （P16370） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 1 （P20434） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 1 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 1 （P20436） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 1 （P27999） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 1 （P22139） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 1 （P38902） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 1 （P40422） ZINC ION × 8 MANGANESE (II) ION × 1 water × 7	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 20	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 2 （P04050） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 2 （P16370） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 2 （P20434） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 2 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 2 （P20436） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 2 （P27999） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 2 （P22139） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 2 （P38902） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 2 （P40422） ZINC ION × 16 MANGANESE (II) ION × 2 water × 14	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB2_YEAST
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–1224； UniProt 1–1224

DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE

OrganismNot specified

UniProt P16370

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 10	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 1 （P04050） DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 1 （P08518） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 1 （P20434） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 1 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 1 （P20436） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 1 （P27999） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 1 （P22139） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 1 （P38902） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 1 （P40422） ZINC ION × 8 MANGANESE (II) ION × 1 water × 7	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 20	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 2 （P04050） DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 2 （P08518） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 2 （P20434） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 2 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 2 （P20436） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 2 （P27999） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 2 （P22139） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 2 （P38902） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 2 （P40422） ZINC ION × 16 MANGANESE (II) ION × 2 water × 14	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB3_YEAST
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–318； UniProt 1–318

DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE

OrganismNot specified

UniProt P20434

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 10	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 1 （P04050） DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 1 （P08518） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 1 （P16370） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 1 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 1 （P20436） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 1 （P27999） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 1 （P22139） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 1 （P38902） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 1 （P40422） ZINC ION × 8 MANGANESE (II) ION × 1 water × 7	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 20	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 2 （P04050） DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 2 （P08518） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 2 （P16370） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 2 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 2 （P20436） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 2 （P27999） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 2 （P22139） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 2 （P38902） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 2 （P40422） ZINC ION × 16 MANGANESE (II) ION × 2 water × 14	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB5_YEAST
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain E； PDBConstruct 1–215； UniProt 1–215

DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE

OrganismNot specified

UniProt P20435

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 10	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 1 （P04050） DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 1 （P08518） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 1 （P16370） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 1 （P20434） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 1 （P20436） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 1 （P27999） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 1 （P22139） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 1 （P38902） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 1 （P40422） ZINC ION × 8 MANGANESE (II) ION × 1 water × 7	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 20	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 2 （P04050） DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 2 （P08518） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 2 （P16370） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 2 （P20434） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 2 （P20436） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 2 （P27999） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 2 （P22139） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 2 （P38902） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 2 （P40422） ZINC ION × 16 MANGANESE (II) ION × 2 water × 14	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB6_YEAST
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain F； PDBConstruct 1–155； UniProt 1–155

DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE

OrganismNot specified

UniProt P20436

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 10	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 1 （P04050） DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 1 （P08518） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 1 （P16370） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 1 （P20434） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 1 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 1 （P27999） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 1 （P22139） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 1 （P38902） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 1 （P40422） ZINC ION × 8 MANGANESE (II) ION × 1 water × 7	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 20	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 2 （P04050） DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 2 （P08518） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 2 （P16370） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 2 （P20434） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 2 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 2 （P27999） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 2 （P22139） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 2 （P38902） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 2 （P40422） ZINC ION × 16 MANGANESE (II) ION × 2 water × 14	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB8_YEAST
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain H； PDBConstruct 1–146； UniProt 1–146

DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE

OrganismNot specified

UniProt P27999

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 10	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 1 （P04050） DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 1 （P08518） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 1 （P16370） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 1 （P20434） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 1 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 1 （P20436） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 1 （P22139） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 1 （P38902） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 1 （P40422） ZINC ION × 8 MANGANESE (II) ION × 1 water × 7	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 20	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 2 （P04050） DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 2 （P08518） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 2 （P16370） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 2 （P20434） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 2 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 2 （P20436） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 2 （P22139） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 2 （P38902） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 2 （P40422） ZINC ION × 16 MANGANESE (II) ION × 2 water × 14	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB9_YEAST
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain I； PDBConstruct 1–122； UniProt 1–122

DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE

OrganismNot specified

UniProt P22139

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 10	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 1 （P04050） DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 1 （P08518） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 1 （P16370） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 1 （P20434） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 1 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 1 （P20436） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 1 （P27999） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 1 （P38902） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 1 （P40422） ZINC ION × 8 MANGANESE (II) ION × 1 water × 7	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 20	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 2 （P04050） DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 2 （P08518） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 2 （P16370） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 2 （P20434） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 2 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 2 （P20436） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 2 （P27999） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 2 （P38902） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 2 （P40422） ZINC ION × 16 MANGANESE (II) ION × 2 water × 14	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB10_YEAST
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain J； PDBConstruct 1–70； UniProt 1–70

DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE

OrganismNot specified

UniProt P38902

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 10	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 1 （P04050） DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 1 （P08518） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 1 （P16370） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 1 （P20434） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 1 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 1 （P20436） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 1 （P27999） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 1 （P22139） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 1 （P40422） ZINC ION × 8 MANGANESE (II) ION × 1 water × 7	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 20	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 2 （P04050） DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 2 （P08518） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 2 （P16370） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 2 （P20434） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 2 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 2 （P20436） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 2 （P27999） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 2 （P22139） DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE × 2 （P40422） ZINC ION × 16 MANGANESE (II) ION × 2 water × 14	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB11_YEAST
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain K； PDBConstruct 1–120； UniProt 1–120

DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE

OrganismNot specified

UniProt P40422

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 10	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 1 （P04050） DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 1 （P08518） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 1 （P16370） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 1 （P20434） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 1 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 1 （P20436） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 1 （P27999） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 1 （P22139） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 1 （P38902） ZINC ION × 8 MANGANESE (II) ION × 1 water × 7	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 20	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT × 2 （P04050） DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE × 2 （P08518） DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE × 2 （P16370） DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE × 2 （P20434） DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE × 2 （P20435） DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE × 2 （P20436） DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE × 2 （P27999） DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE × 2 （P22139） DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE × 2 （P38902） ZINC ION × 16 MANGANESE (II) ION × 2 water × 14	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPC10_YEAST
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain L； PDBConstruct 1–70； UniProt 1–70

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1i50
Deposition date deposition_date	2001-02-23
Structure title title	RNA POLYMERASE II CRYSTAL FORM II AT 2.8 A RESOLUTION
Keywords keywords	TRANSCRIPTION, MRNA, MULTIPROTEIN COMPLEX, MOLECULAR MACHINE, ZINC MOTIFS; TRANSCRIPTION
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1i50__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1i50__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

1i50__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	49.39 Å
Rg (electron density)	48.53 Å
Total Rg	48.84 Å
Atom count	28288
Residues	3558
Excluded volume	504710 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`1i50__assembly_1__model_1`	decameric （10）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`1i50__assembly_2__model_1`	eicosameric （20）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (13)

Entity 1 Entity 1 polymer

Description description	DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT
Molecular weight formula_weight	191821.6 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	28366

MVGQQYSSAPLRTVKEVQFGLFSPEEVRAISVAKIRFPETMDETQTRAKIGGLNDPRLGSIDRNLKCQTCQEGMNECPGHFGHIDLAKPVFHVGFIAKIKKVCECVCMHCGKLLLDEHNELMRQALAIKDSKKRFAAIWTLCKTKMVCETDVPSEDDPTQLVSRGGCGNTQPTIRKDGLKLVGSWKKDRATGDADEPELRVLSTEEILNIFKHISVKDFTSLGFNEVFSRPEWMILTCLPVPPPPVRPSISFNESQRGEDDLTFKLADILKANISLETLEHNGAPHHAIEEAESLLQFHVATYMDNDIAGQPQALQKSGRPVKSIRARLKGKEGRIRGNLMGKRVDFSARTVISGDPNLELDQVGVPKSIAKTLTYPEVVTPYNIDRLTQLVRNGPNEHPGAKYVIRDSGDRIDLRYSKRAGDIQLQYGWKVERHIMDNDPVLFNRQPSLHKMSMMAHRVKVIPYSTFRLNLSVTSPYNADFDGDEMNLHVPQSEETRAELSQLCAVPLQIVSPQSNKPCMGIVQDTLCGIRKLTLRDTFIELDQVLNMLYWVPDWDGVIPTPAIIKPKPLWSGKQILSVAIPNGIHLQRFDEGTTLLSPKDNGMLIIDGQIIFGVVEKKTVGSSNGGLIHVVTREKGPQVCAKLFGNIQKVVNFWLLHNGFSTGIGDTIADGPTMREITETIAEAKKKVLDVTKEAQANLLTAKHGMTLRESFEDNVVRFLNEARDKAGRLAEVNLKDLNNVKQMVMAGSKGSFINIAQMSACVGQQSVEGKRIAFGFVDRTLPHFSKDDYSPESKGFVENSYLRGLTPQEFFFHAMGGREGLIDTAVKTAETGYIQRRLVKALEDIMVHYDNTTRNSLGNVIQFIYGEDGMDAAHIEKQSLDTIGGSDAAFEKRYRVDLLNTDHTLDPSLLESGSEILGDLKLQVLLDEEYKQLVKDRKFLREVFVDGEANWPLPVNIRRIIQNAQQTFHIDHTKPSDLTIKDIVLGVKDLQENLLVLRGKNEIIQNAQRDAVTLFCCLLRSRLATRRVLQEYRLTKQAFDWVLSNIEAQFLRSVVHPGEMVGVLAAQSIGEPATQMTLNTFHFAGVASKKVTSGVPRLKEILNVAKNMKTPSLTVYLEPGHAADQEQAKLIRSAIEHTTLKSVTIASEIYYDPDPRSTVIPEDEEIIQLHFSLLDEEAEQSFDQQSPWLLRLELDRAAMNDKDLTMGQVGERIKQTFKNDLFVIWSEDNDEKLIIRCRVVRPKSLDAETEAEEDHMLKKIENTMLENITLRGVENIERVVMMKYDRKVPSPTGEYVKEPEWVLETDGVNLSEVMTVPGIDPTRIYTNSFIDIMEVLGIEAGRAALYKEVYNVIASDGSYVNYRHMALLVDVMTTQGGLTSVTRHGFNRSNTGALMRCSFEETVEILFEAGASAELDDCRGVSENVILGQMAPIGTGAFDVMIDEESLVKYMPEQKITEIEDGQDGGVTPYSNESGLVNADLDVKDELMFSPLVDSGSNDAMAGGFTAYGGADYGEATSPFGAYGEAPTSPGFGVSSPGFSPTSPTYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPGYSPGSPAYSPKQDEQKHNENENSR

Entity 2 Entity 2 polymer

Description description	DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE
Molecular weight formula_weight	138937.3 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

MSDLANSEKYYDEDPYGFEDESAPITAEDSWAVISAFFREKGLVSQQLDSFNQFVDYTLQDIICEDSTLILEQLAQHTTESDNISRKYEISFGKIYVTKPMVNESDGVTHALYPQEARLRNLTYSSGLFVDVKKRTYEAIDVPGRELKYELIAEESEDDSESGKVFIGRLPIMLRSKNCYLSEATESDLYKLKECPFDMGGYFIINGSEKVLIAQERSAGNIVQVFKKAAPSPISHVAEIRSALEKGSRFISTLQVKLYGREGSSARTIKATLPYIKQDIPIVIIFRALGIIPDGEILEHICYDVNDWQMLEMLKPCVEDGFVIQDRETALDFIGRRGTALGIKKEKRIQYAKDILQKEFLPHITQLEGFESRKAFFLGYMINRLLLCALDRKDQDDRDHFGKKRLDLAGPLLAQLFKTLFKKLTKDIFRYMQRTVEEAHDFNMKLAINAKTITSGLKYALATGNWGEQKKAMSSRAGVSQVLNRYTYSSTLSHLRRTNTPIGRDGKLAKPRQLHNTHWGLVCPAETPEGQACGLVKNLSLMSCISVGTDPMPIITFLSEWGMEPLEDYVPHQSPDATRVFVNGVWHGVHRNPARLMETLRTLRRKGDINPEVSMIRDIREKELKIFTDAGRVYRPLFIVEDDESLGHKELKVRKGHIAKLMATEYQDIEGGFEDVEEYTWSSLLNEGLVEYIDAEEEESILIAMQPEDLEPAEANEENDLDVDPAKRIRVSHHATTFTHCEIHPSMILGVAASIIPFPDHNQSPRNTYQSAMGKQAMGVFLTNYNVRMDTMANILYYPQKPLGTTRAMEYLKFRELPAGQNAIVAIACYSGYNQEDSMIMNQSSIDRGLFRSLFFRSYMDQEKKYGMSITETFEKPQRTNTLRMKHGTYDKLDDDGLIAPGVRVSGEDVIIGKTTPISPDEEELGQRTAYHSKRDASTPLRSTENGIVDQVLVTTNQDGLKFVKVRVRTTKIPQIGDKFASRHGQKGTIGITYRREDMPFTAEGIVPDLIINPHAIPSRMTVAHLIECLLSKVAALSGNEGDASPFTDITVEGISKLLREHGYQSRGFEVMYNGHTGKKLMAQIFFGPTYYQRLRHMVDDKIHARARGPMQVLTRQPVEGRSRDGGLRFGEMERDCMIAHGAASFLKERLMEASDAFRVHICGICGLMTVIAKLNHNQFECKGCDNKIDIYQIHIPYAAKLLFQELMAMNITPRLYTDRSRDF

Entity 3 Entity 3 polymer

Description description	DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE
Molecular weight formula_weight	35330.5 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

MSEEGPQVKIREASKDNVDFILSNVDLAMANSLRRVMIAEIPTLAIDSVEVETNTTVLADEFIAHRLGLIPLQSMDIEQLEYSRDCFCEDHCDKCSVVLTLQAFGESESTTNVYSKDLVIVSNLMGRNIGHPIIQDKEGNGVLICKLRKGQELKLTCVAKKGIAKEHAKWGPAAAIEFEYDPWNKLKHTDYWYEQDSAKEWPQSKNCEYEDPPNEGDPFDYKAQADTFYMNVESVGSIPVDQVVVRGIDTLQKKVASILLALTQMDQDKVNFASGDNNTASNMLGSNEDVMMTGAEQDPYSNASQMGNTGSGGYDNAW

Entity 4 Entity 4 polymer

Description description	DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE
Molecular weight formula_weight	25117.1 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

MDQENERNISRLWRAFRTVKEMVKDRGYFITQEEVELPLEDFKAKYCDSMGRPQRKMMSFQANPTEESISKFPDMGSLWVEFCDEPSVGVKTMKTFVIHIQEKNFQTGIFVYQNNITPSAMKLVPSIPPATIETFNEAALVVNITHHELVPKHIRLSSDEKRELLKRYRLKESQLPRIQRADPVALYLGLKRGEVVKIIRKSETSGRYASYRICM

Entity 5 Entity 5 polymer

Description description	DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE
Molecular weight formula_weight	17931.8 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQHEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL

Entity 6 Entity 6 polymer

Description description	DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE
Molecular weight formula_weight	16525.4 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

MSNTLFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIASSLNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSFGGLLMRLEGNYRNLNNLKQENAYLLIRR

Entity 7 Entity 7 polymer

Description description	DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE
Molecular weight formula_weight	14308.2 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

MTTFRFCRDCNNMLYPREDKENNRLLFECRTCSYVEEAGSPLVYRHELITNIGETAGVVQDIGSDPTLPRSDRECPKCHSRENVFFQSQQRRKDTSMVLFFVCLSCSHIFTSDQKNKRTQFS

Entity 8 Entity 8 polymer

Description description	DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE
Molecular weight formula_weight	8290.7 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKFLRYNPLEKRD

Entity 9 Entity 9 polymer

Description description	DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE
Molecular weight formula_weight	13633.5 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

MNAPDRFELFLLGEGESKLKIDPDTKAPNAVVITFEKEDHTLGNLIRAELLNDRKVLFAAYKVEHPFFARFKLRIQTTEGYDPKDALKNACNSIINKLGALKTNFETEWNLQTLAADDAF

Entity 10 Entity 10 polymer

Description description	DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE
Molecular weight formula_weight	7730.0 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L

MSREGFQIPTNLDAAAAGTSQARTATLKYICAECSSKLSLSRTDAVRCKDCGHRILLKARTKRLVQFEAR

Entity 11 Entity 11 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	8

Entity 12 Entity 12 non-polymer

Description description	MANGANESE (II) ION
Molecular weight formula_weight	54.9 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 13 Entity 13 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	78

▼

6. Fold Classification (SCOP + CATH) 34 domains

SCOP 2.08 (13 domains)

Domain ID domain_id	d1i50a_
Class class	e — Multi-domain proteins (alpha and beta)
Fold Fold fold	e.29 — beta and beta-prime subunits of DNA dependent RNA-polymerase
Superfamily Superfamily superfamily	e.29.1 — beta and beta-prime subunits of DNA dependent RNA-polymerase
Family Family family	e.29.1.2 — RNA-polymerase beta-prime

Domain ID domain_id	d1i50b_
Class class	e — Multi-domain proteins (alpha and beta)
Fold Fold fold	e.29 — beta and beta-prime subunits of DNA dependent RNA-polymerase
Superfamily Superfamily superfamily	e.29.1 — beta and beta-prime subunits of DNA dependent RNA-polymerase
Family Family family	e.29.1.1 — RNA-polymerase beta

Domain ID domain_id	d1i50c1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.74 — DCoH-like
Superfamily Superfamily superfamily	d.74.3 — RBP11-like subunits of RNA polymerase
Family Family family	d.74.3.1 — RNA polymerase alpha subunit dimerisation domain

Domain ID domain_id	d1i50c2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.181 — Insert subdomain of RNA polymerase alpha subunit
Superfamily Superfamily superfamily	d.181.1 — Insert subdomain of RNA polymerase alpha subunit
Family Family family	d.181.1.1 — Insert subdomain of RNA polymerase alpha subunit

Domain ID domain_id	d1i50e1
Class class	c — Alpha and beta proteins (a/b)
Fold Fold fold	c.52 — Restriction endonuclease-like
Superfamily Superfamily superfamily	c.52.3 — Eukaryotic RPB5 N-terminal domain
Family Family family	c.52.3.1 — Eukaryotic RPB5 N-terminal domain

Domain ID domain_id	d1i50e2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.78 — RPB5-like RNA polymerase subunit
Superfamily Superfamily superfamily	d.78.1 — RPB5-like RNA polymerase subunit
Family Family family	d.78.1.1 — RPB5

Domain ID domain_id	d1i50f_
Class class	a — All alpha proteins
Fold Fold fold	a.143 — RPB6/omega subunit-like
Superfamily Superfamily superfamily	a.143.1 — RPB6/omega subunit-like
Family Family family	a.143.1.2 — RPB6

Domain ID domain_id	d1i50h_
Class class	b — All beta proteins
Fold Fold fold	b.40 — OB-fold
Superfamily Superfamily superfamily	b.40.4 — Nucleic acid-binding proteins
Family Family family	b.40.4.8 — RNA polymerase subunit RBP8

Domain ID domain_id	d1i50i1
Class class	g — Small proteins
Fold Fold fold	g.41 — Rubredoxin-like
Superfamily Superfamily superfamily	g.41.3 — Zinc beta-ribbon
Family Family family	g.41.3.1 — Transcriptional factor domain

Domain ID domain_id	d1i50i2
Class class	g — Small proteins
Fold Fold fold	g.41 — Rubredoxin-like
Superfamily Superfamily superfamily	g.41.3 — Zinc beta-ribbon
Family Family family	g.41.3.1 — Transcriptional factor domain

Domain ID domain_id	d1i50j_
Class class	a — All alpha proteins
Fold Fold fold	a.4 — DNA/RNA-binding 3-helical bundle
Superfamily Superfamily superfamily	a.4.11 — RNA polymerase subunit RPB10
Family Family family	a.4.11.1 — RNA polymerase subunit RPB10

Domain ID domain_id	d1i50k_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.74 — DCoH-like
Superfamily Superfamily superfamily	d.74.3 — RBP11-like subunits of RNA polymerase
Family Family family	d.74.3.2 — RBP11/RpoL

Domain ID domain_id	d1i50l_
Class class	g — Small proteins
Fold Fold fold	g.41 — Rubredoxin-like
Superfamily Superfamily superfamily	g.41.9 — RNA polymerase subunits
Family Family family	g.41.9.2 — RBP12 subunit of RNA polymerase II

CATH v4.4 (21 domains)

Domain ID domain_id	1i50A03
Class class	2 — Mainly Beta
Architecture architecture	40 — Beta Barrel
Topology topology	40 — Barwin-like endoglucanases
Homologous superfamily homologous superfamily	20 —

Domain ID domain_id	1i50A04
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1490 — Dna Ligase; domain 1
Homologous superfamily homologous superfamily	180 — RNA polymerase ii

Domain ID domain_id	1i50A05
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	274 — Enzyme I; Chain A, domain 2
Homologous superfamily homologous superfamily	100 — RNA polymerase Rpb1, domain 3

Domain ID domain_id	1i50A06
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	132 — Topoisomerase I; Chain A, domain 4
Homologous superfamily homologous superfamily	30 — RNA polymerase Rpb1 funnel domain

Domain ID domain_id	1i50A09
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1360 — Gyrase A; domain 2
Homologous superfamily homologous superfamily	140 —

Domain ID domain_id	1i50B04
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1110 — Dna-directed Rna Polymerase Ii 140kd Polypeptide; Chain: B; domain 3
Homologous superfamily homologous superfamily	10 — RNA polymerase Rpb2, domain 2

Domain ID domain_id	1i50B05
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1070 — Hypothetical Protein Ta0175; Chain: A, domain 2
Homologous superfamily homologous superfamily	20 —

Domain ID domain_id	1i50B06
Class class	2 — Mainly Beta
Architecture architecture	40 — Beta Barrel
Topology topology	270 — Dna-directed Rna Polymerase Ii 140kd Polypeptide; Chain: B; Domain 6
Homologous superfamily homologous superfamily	10 — DNA-directed RNA polymerase, subunit 2, domain 6

Domain ID domain_id	1i50B07
Class class	2 — Mainly Beta
Architecture architecture	40 — Beta Barrel
Topology topology	50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Homologous superfamily homologous superfamily	150 — RNA polymerase II, Rpb2 subunit, wall domain

Domain ID domain_id	1i50B08
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	1800 — DCoH-like
Homologous superfamily homologous superfamily	10 — RNA polymerase alpha subunit dimerisation domain

Domain ID domain_id	1i50C01
Class class	2 — Mainly Beta
Architecture architecture	170 — Beta Complex
Topology topology	120 — RNA Polymerase Alpha Subunit; Chain A, domain 2
Homologous superfamily homologous superfamily	12 — DNA-directed RNA polymerase, insert domain

Domain ID domain_id	1i50C02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1360 — Gyrase A; domain 2
Homologous superfamily homologous superfamily	10 — RNA polymerase, RBP11-like subunit

Domain ID domain_id	1i50E01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	1340 — Dna-directed Rna Polymerases I, Ii, And Iii 27 Kd Polypeptide; Chain: A; domain 1
Homologous superfamily homologous superfamily	10 — RNA polymerase, Rpb5, N-terminal domain

Domain ID domain_id	1i50E02
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	940 — Eukaryotic RPB6 RNA polymerase subunit
Homologous superfamily homologous superfamily	20 — RPB5-like RNA polymerase subunit

Domain ID domain_id	1i50F00
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	940 — Eukaryotic RPB6 RNA polymerase subunit
Homologous superfamily homologous superfamily	10 — RNA polymerase subunit, RPB6/omega

Domain ID domain_id	1i50H00
Class class	2 — Mainly Beta
Architecture architecture	40 — Beta Barrel
Topology topology	50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Homologous superfamily homologous superfamily	140 — Nucleic acid-binding proteins

Domain ID domain_id	1i50I01
Class class	2 — Mainly Beta
Architecture architecture	20 — Single Sheet
Topology topology	25 — N-terminal domain of TfIIb
Homologous superfamily homologous superfamily	10 —

Domain ID domain_id	1i50I02
Class class	2 — Mainly Beta
Architecture architecture	20 — Single Sheet
Topology topology	25 — N-terminal domain of TfIIb
Homologous superfamily homologous superfamily	10 —

Domain ID domain_id	1i50J00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	10 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily	60 — Homeodomain-like

Domain ID domain_id	1i50K00
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1360 — Gyrase A; domain 2
Homologous superfamily homologous superfamily	10 — RNA polymerase, RBP11-like subunit

Domain ID domain_id	1i50L00
Class class	2 — Mainly Beta
Architecture architecture	20 — Single Sheet
Topology topology	28 — Rubrerythrin, domain 2
Homologous superfamily homologous superfamily	30 — RNA polymerase ii, chain L

▶

Unit-cell length a length_a	122.700 Å
Unit-cell length b length_b	223.000 Å
Unit-cell length c length_c	376.100 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	8

Space group space_group	I 2 2 2
Space-group number int_tables_number	23
Crystal system crystal_system	—

Resolution resolution	2.800 Å
Low-resolution limit resolution_low	40.000 Å
R factor r_factor	—
R_work r_work	0.2290
R_free r_free	0.2820

RNA POLYMERASE II CRYSTAL FORM II AT 2.8 A RESOLUTION

1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASE II 140KD POLYPEPTIDE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer