Current Protein Identity：P41594
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3LMK Ligand Binding Domain of Metabotropoc glutamate receptor mGluR5 complexed with glutamate Deposited 2010-01-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
18–505（488 aa）
Fragment：Ligand binding domain
Chain B
18–505（488 aa）
Fragment：Ligand binding domain
|
Mutation：C241S Mutation：C241S | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 GLU GLUTAMIC ACID × 2 MG MAGNESIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；25% PEG 8000, 0.2M NaCl, 0.1M Hepes. protein concentration 5mg/mL plus 5mM L-Glu. Cryoprotectant used: 33% PEG 8000, 0.2M NaCl and 0.1M Hepes plus 20% Glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.44 Å R-free 0.281 |
| 4OO9 Structure of the human class C GPCR metabotropic glutamate receptor 5 transmembrane domain in complex with the negative allosteric modulator mavoglurant Deposited 2014-01-31 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
569–678（110 aa）
Fragment：SEE REMARK 999
Chain A
680–836（157 aa）
Fragment：SEE REMARK 999
|
Mutation：yes Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：yes Non-standard monomer：Yes (specific site not provided by mmCIF) | OLA OLEIC ACID × 4 2U8 Mavoglurant × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.8；293.1 K；24-34% v/v PEG400, 0.2 M ammonium phosphate dibasic, 0.1 M MES, pH 6.8, LIPIDIC CUBIC PHASE, temperature 293.1K
|
Resolution 2.60 Å R-free 0.275 |
| 5CGC Structure of the human class C GPCR metabotropic glutamate receptor 5 transmembrane domain in complex with the negative allosteric modulator 3-chloro-4-fluoro-5-[6-(1H-pyrazol-1-yl)pyrimidin-4-yl]benzonitrile Deposited 2015-07-09 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
569–678（110 aa）
Chain A
679–836（158 aa）
|
Mutation：;E579A N667Y I669A G675M C1054T C1097A T742A S753A,E579A N667Y I669A G675M C1054T C1097A T742A S753A,E579A N667Y I669A G675M C1054T C1097A T742A S753A ; Mutation：;E579A N667Y I669A G675M C1054T C1097A T742A S753A,E579A N667Y I669A G675M C1054T C1097A T742A S753A,E579A N667Y I669A G675M C1054T C1097A T742A S753A ; | OLA OLEIC ACID × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 51D 3-chloro-4-fluoro-5-[6-(1H-pyrazol-1-yl)pyrimidin-4-yl]benzonitrile × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.8；293.1 K；24-34% V/V PEG400, 0.2 M AMMONIUM PHOSPHATE DIBASIC, 0.1 M MES, PH 6.8,
|
Resolution 3.10 Å R-free 0.287 |
| 5CGD Structure of the human class C GPCR metabotropic glutamate receptor 5 transmembrane domain in complex with the negative allosteric modulator 3-chloro-5-[6-(5-fluoropyridin-2-yl)pyrimidin-4-yl]benzonitrile - (HTL14242) Deposited 2015-07-09 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
569–678（110 aa）
Chain A
679–836（158 aa）
|
Mutation：;E579A N667Y I669A G675M C1054T C1097A T742A S753A,E579A N667Y I669A G675M C1054T C1097A T742A S753A,E579A N667Y I669A G675M C1054T C1097A T742A S753A ; Mutation：;E579A N667Y I669A G675M C1054T C1097A T742A S753A,E579A N667Y I669A G675M C1054T C1097A T742A S753A,E579A N667Y I669A G675M C1054T C1097A T742A S753A ; | OLA OLEIC ACID × 4 51E 3-chloro-5-[6-(5-fluoropyridin-2-yl)pyrimidin-4-yl]benzonitrile × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.8；293.1 K；24-34% V/V PEG400, 0.2 M AMMONIUM PHOSPHATE DIBASIC, 0.1 M MES, PH 6.8,
|
Resolution 2.60 Å R-free 0.285 |
| 6FFH Crystal Structure of mGluR5 in complex with Fenobam at 2.65 A Deposited 2018-01-08 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
569–678（110 aa）
Fragment：MGLUR5
Chain A
679–836（158 aa）
Fragment：MGLUR5
|
Mutation：C54T C97A E579A N667Y I669A G675M T742A S753A Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：C54T C97A E579A N667Y I669A G675M T742A S753A Non-standard monomer：Yes (specific site not provided by mmCIF) | OLA OLEIC ACID × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 D7W 1-(3-chlorophenyl)-3-(3-methyl-5-oxidanylidene-4~{H}-imidazol-2-yl)urea × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.8；293.1 K；24-34% V/V PEG400, 0.2 M AMMONIUM PHOSPHATE DIBASIC, 0.1 M MES, PH 6.8
|
Resolution 2.65 Å R-free 0.267 |
| 6FFI Crystal Structure of mGluR5 in complex with MMPEP at 2.2 A Deposited 2018-01-08 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
569–678（110 aa）
Fragment：MGLUR5,MGLUR5,MGLUR5,MGLUR5,MGLUR5,MGLUR5,MGLUR5,MGLUR5,MGLUR5
Chain A
680–836（157 aa）
Fragment：MGLUR5,MGLUR5,MGLUR5,MGLUR5,MGLUR5,MGLUR5,MGLUR5,MGLUR5,MGLUR5
|
Mutation：;E579A N667Y I669A G675M T742A S753A,E579A N667Y I669A G675M T742A S753A,E579A N667Y I669A G675M T742A S753A,E579A N667Y I669A G675M T742A S753A,E579A N667Y I669A G675M T742A S753A,E579A N667Y I669A G675M T742A S753A,E579A N667Y I669A G675M T742A S753A,E579A N667Y I669A G675M T742A S753A,E579A N667Y I669A G675M T742A S753A ; Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：;E579A N667Y I669A G675M T742A S753A,E579A N667Y I669A G675M T742A S753A,E579A N667Y I669A G675M T742A S753A,E579A N667Y I669A G675M T742A S753A,E579A N667Y I669A G675M T742A S753A,E579A N667Y I669A G675M T742A S753A,E579A N667Y I669A G675M T742A S753A,E579A N667Y I669A G675M T742A S753A,E579A N667Y I669A G675M T742A S753A ; Non-standard monomer：Yes (specific site not provided by mmCIF) | OLA OLEIC ACID × 7 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 D8B 2-[2-(3-methoxyphenyl)ethynyl]-6-methyl-pyridine × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.8；293.1 K；24-34% V/V PEG400, 0.2 M AMMONIUM PHOSPHATE DIBASIC, 0.1 M MES, PH 6.8
|
Resolution 2.20 Å R-free 0.269 |
| 6N4X Metabotropic Glutamate Receptor 5 Apo Form Ligand Binding Domain Deposited 2018-11-20 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
20–865（846 aa）
Chain B
20–865（846 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；15% PEG 3350, 0.2M Na Cacodylate pH 6.5
|
Resolution 4.00 Å R-free 0.282 |
| 6N4Y Metabotropic Glutamate Receptor 5 Extracellular Domain with Nb43 Deposited 2018-11-20 | Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
21–571（551 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；18-20% PEG 3350
0.15M Potassium Nitrate
1% Benzamidine
|
Resolution 3.26 Å R-free 0.251 |
| 6N4Y Metabotropic Glutamate Receptor 5 Extracellular Domain with Nb43 Deposited 2018-11-20 | Assembly 2 Other combination Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
21–571（551 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；18-20% PEG 3350
0.15M Potassium Nitrate
1% Benzamidine
|
Resolution 3.26 Å R-free 0.251 |
| 6N4Y Metabotropic Glutamate Receptor 5 Extracellular Domain with Nb43 Deposited 2018-11-20 | Assembly 3 Other combination Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
21–571（551 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；18-20% PEG 3350
0.15M Potassium Nitrate
1% Benzamidine
|
Resolution 3.26 Å R-free 0.251 |
| 6N4Y Metabotropic Glutamate Receptor 5 Extracellular Domain with Nb43 Deposited 2018-11-20 | Assembly 4 Other combination Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
21–571（551 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；18-20% PEG 3350
0.15M Potassium Nitrate
1% Benzamidine
|
Resolution 3.26 Å R-free 0.251 |
| 6N50 Metabotropic Glutamate Receptor 5 Extracellular Domain in Complex with Nb43 and L-quisqualic acid Deposited 2018-11-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
21–571（551 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 QUS (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM NaCl
50 mM ADA pH 7.0
20% PEG 4000
|
Resolution 3.75 Å R-free 0.300 |
| 6N50 Metabotropic Glutamate Receptor 5 Extracellular Domain in Complex with Nb43 and L-quisqualic acid Deposited 2018-11-20 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
21–571（551 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 QUS (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM NaCl
50 mM ADA pH 7.0
20% PEG 4000
|
Resolution 3.75 Å R-free 0.300 |
| 6N50 Metabotropic Glutamate Receptor 5 Extracellular Domain in Complex with Nb43 and L-quisqualic acid Deposited 2018-11-20 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
21–571（551 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 QUS (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM NaCl
50 mM ADA pH 7.0
20% PEG 4000
|
Resolution 3.75 Å R-free 0.300 |
| 6N51 Metabotropic Glutamate Receptor 5 bound to L-quisqualate and Nb43 Deposited 2018-11-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
23–826（804 aa）
Chain B
23–826（804 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 QUS (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；additional 0.1%OG was added to the sample right before applying sample to grid;
3.5ul sample was applied;
blot for 1 second before plunging;
|
Resolution 4.00 Å |
| 6N52 Metabotropic Glutamate Receptor 5 Apo Form Deposited 2018-11-20 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
20–839（820 aa）
Chain B
20–839（820 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；100mM NaCl, 20 mM HEPES pH 7.5, 5uM FFMTEB added as a negative allosteric modulator.
cryo-EM vitrification conditions
Cryogen ETHANE；Addition 0.0005% Amphipol A8-35was added to sample prior to apply to grid;
3.5ul sample was applied;
blot for 1s before plunging
|
Resolution 4.00 Å |
| 7FD8 Thermostabilised full length human mGluR5-5M bound with L-quisqualic acid Deposited 2021-07-16 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
21–856（836 aa）
Chain B
21–856（836 aa）
|
Mutation：H350L, N445A, T742A, S753A, T777A, I799A, A813L Mutation：H350L, N445A, T742A, S753A, T777A, I799A, A813L | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 QUS (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID × 2 Y01 CHOLESTEROL HEMISUCCINATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force was 10, 3.5 seconds blotting time
|
Resolution 3.80 Å |
| 7FD9 Thermostabilised full length human mGluR5-5M with orthosteric antagonist, LY341495 Deposited 2021-07-16 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
21–856（836 aa）
Chain B
21–856（836 aa）
|
Mutation：H350L, N445A, T742A, S753A, T777A, I799A, A813L Mutation：H350L, N445A, T742A, S753A, T777A, I799A, A813L | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 Z99 2-[(1S,2S)-2-carboxycyclopropyl]-3-(9H-xanthen-9-yl)-D-alanine × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force was 10, 3.5 seconds blotting time
|
Resolution 4.00 Å |
| 7P2L thermostabilised 7TM domain of human mGlu5 receptor bound to photoswitchable ligand alloswitch-1 Deposited 2021-07-06 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
569–678（110 aa）
Chain A
679–836（158 aa）
|
Mutation：E579A,N667Y,I669A,G675M,T742A,S753A Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：E579A,N667Y,I669A,G675M,T742A,S753A Non-standard monomer：Yes (specific site not provided by mmCIF) | 4YI 2-chloranyl-~{N}-[2-methoxy-4-[(~{E})-pyridin-2-yldiazenyl]phenyl]benzamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；295 K；0.15-0.25 M ammonium phosphate dibasic, 22-24 % polyethylene glycol 400, either with 0.10 M 2-(N-morpholino)ethanesulfonic acid (MES) pH 6.7-6.8 or 0.1 M HEPES pH 6.8
|
Resolution 2.54 Å R-free 0.285 |
| 8T6J CDPPB-bound inactive mGlu5 Deposited 2023-06-16 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
20–876（857 aa）
Chain B
20–876（857 aa）
|
Not recorded | YKU 3-cyano-N-(1,3-diphenyl-1H-pyrazol-5-yl)benzamide × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8T7H Quis-bound intermediate mGlu5 Deposited 2023-06-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–876（857 aa）
Chain B
20–876（857 aa）
|
Not recorded | QUS (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8T8M Quis-bound intermediate mGlu5 Deposited 2023-06-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–876（857 aa）
Chain B
20–876（857 aa）
|
Not recorded | QUS (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8TAO Quis and CDPPB bound active mGlu5 Deposited 2023-06-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
20–876（857 aa）
Chain B
20–876（857 aa）
|
Not recorded | QUS (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID × 2 YKU 3-cyano-N-(1,3-diphenyl-1H-pyrazol-5-yl)benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8X0B Human FL Metabotropic glutamate receptor 5, mGlu5-5M with Quisqualate and VU0424465 Deposited 2023-11-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
21–856（836 aa）
Chain B
21–856（836 aa）
|
Mutation：T742A, S753A, T777A, I799A, A813L,N445,H350L Mutation：T742A, S753A, T777A, I799A, A813L,N445,H350L | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 QUS (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID × 2 XQT VU0424465 × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8X0C Human FL Metabotropic glutamate receptor 5, mGlu5-5M with quisqualate and VU0424465, conformer 1 Deposited 2023-11-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
21–856（836 aa）
Chain B
21–856（836 aa）
|
Mutation：T742A,S753A,T777A,I799A,A813L,N455A,H350L Mutation：T742A,S753A,T777A,I799A,A813L,N455A,H350L | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 QUS (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID × 2 XQT VU0424465 × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotting time was 5 seconds, blot force of 10
|
Resolution 3.20 Å |
| 8X0D Human FL Metabotropic glutamate receptor 5, mGlu5-5M with quisqualate and VU0424465, conformer 2 Deposited 2023-11-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
21–856（836 aa）
Chain B
21–856（836 aa）
|
Mutation：T742A,S753A,T777A,I799A,A813L,N445A,H350L Mutation：T742A,S753A,T777A,I799A,A813L,N445A,H350L | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 QUS (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID × 2 XQT VU0424465 × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotting time of 5 seconds and blot force of 10
|
Resolution 3.50 Å |
| 8X0E Human FL Metabotropic glutamate receptor 5, mGlu5-5M with agonist and PAM, W785A mutant Deposited 2023-11-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
21–856（836 aa）
Chain B
21–856（836 aa）
|
Mutation：T742A,S753A,T777A,I799A,A813L,W785A,H350L Mutation：T742A,S753A,T777A,I799A,A813L,W785A,H350L | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 QUS (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force was set to 0
|
Resolution 3.40 Å |
| 8X0F Human FL Metabotropic glutamate receptor 5, mGlu5-5M with quisqualate and PAM VU29 Deposited 2023-11-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
21–856（836 aa）
Chain B
21–856（836 aa）
|
Mutation：T742A, S753A, T777A, I799A, A813L,N445A,H350L Mutation：T742A, S753A, T777A, I799A, A813L,N445A,H350L | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 QUS (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID × 2 XRQ N-(1,3-diphenyl-1H-pyrazol-5-yl)-4-nitrobenzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8X0G Human FL Metabotropic glutamate receptor 5, mGlu5-5M with quisqualate, Acc conformation (purified with PAM VU0409551 but not modelled) Deposited 2023-11-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
21–856（836 aa）
Chain B
21–856（836 aa）
|
Mutation：T742A, S753A, T777A, I799A, A813L,N445A,H350L Mutation：T742A, S753A, T777A, I799A, A813L,N445A,H350L | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 QUS (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8X0H Human FL Metabotropic glutamate receptor 5, mGlu5-5M with quisqualate, Rcc conformation Deposited 2023-11-04 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
21–856（836 aa）
Chain B
21–856（836 aa）
|
Mutation：T742A, S753A, T777A, I799A, A813L,N445A,H350L Mutation：T742A, S753A, T777A, I799A, A813L,N445A,H350L | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 QUS (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 9HC0 Dark structure of the human metabotropic glutamate receptor 5 transmembrane domain bound to photoswitchable ligand alloswitch-1 Deposited 2024-11-08 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
569–678（110 aa）
Chain A
680–836（157 aa）
|
Mutation：E579A,N667Y,I669A,G675M,T742A,S753A Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：E579A,N667Y,I669A,G675M,T742A,S753A Non-standard monomer：Yes (specific site not provided by mmCIF) | OLA OLEIC ACID × 5 4YI 2-chloranyl-~{N}-[2-methoxy-4-[(~{E})-pyridin-2-yldiazenyl]phenyl]benzamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 7.2；293 K；PEG300, di-ammonium hydrogen phosphate, 1,6-hexanediol, BIS-TRIS
|
Resolution 2.33 Å R-free 0.271 |
| 9HC3 Apo-state structure of the human metabotropic glutamate receptor 5 transmembrane domain freeze-trapped after light activation of photoswitchable ligand alloswitch-1 Deposited 2024-11-08 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
569–678（110 aa）
Chain A
680–836（157 aa）
|
Mutation：E579A,N667Y,I669A,G675M,T742A,S753A Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：E579A,N667Y,I669A,G675M,T742A,S753A Non-standard monomer：Yes (specific site not provided by mmCIF) | OLA OLEIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 7.2；293 K；PEG300, di-ammonium hydrogen phosphate, 1,6-hexanediol, BIS-TRIS
|
Resolution 2.90 Å R-free 0.302 |
| 9WQK GRM5-Gi Complex Structure Deposited 2025-09-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain X
21–839（819 aa）
Chain Y
21–839（819 aa）
|
Not recorded | YKU 3-cyano-N-(1,3-diphenyl-1H-pyrazol-5-yl)benzamide × 1 GGL GAMMA-L-GLUTAMIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CL CHLORIDE ION × 2 CLR CHOLESTEROL × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 9WQL GRM5-Gq Complex Structure Deposited 2025-09-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain X
21–839（819 aa）
Chain Y
21–839（819 aa）
|
Not recorded | YKU 3-cyano-N-(1,3-diphenyl-1H-pyrazol-5-yl)benzamide × 1 GGL GAMMA-L-GLUTAMIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CL CHLORIDE ION × 2 CLR CHOLESTEROL × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |