Current Protein Identity：P53277
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 | Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric（46） Consistent with all polymers |
Chain f
1–215（215 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 5GMK Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution Deposited 2016-07-14 | Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 45-meric（45） Consistent with all polymers |
Chain I
1–215（215 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8；The CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 5MPS Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-18 | Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 30-meric（30） Consistent with all polymers |
Chain y
1–215（215 aa）
|
Not recorded | MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
|
Resolution 3.85 Å |
| 5MQ0 Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-19 | Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 46-meric（46） Consistent with all polymers |
Chain y
1–215（215 aa）
|
Not recorded | MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
|
Resolution 4.17 Å |
| 5WSG Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution Deposited 2016-12-07 | Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 45-meric（45） Consistent with all polymers |
Chain I
1–215（215 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8；10mM Tris-HCl, pH 8.0, 75mM NaCl, 1mM Mg(OAc)2, 1mM imidazole, 0.01% NP40, 1mM TCEP, 0.5mM EGTA
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 5Y88 Cryo-EM structure of the intron-lariat spliceosome ready for disassembly from S.cerevisiae at 3.5 angstrom Deposited 2017-08-20 | Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 44-meric（44） Consistent with all polymers |
Chain K
1–215（215 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.46 Å |
| 5YLZ Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom Deposited 2017-10-20 | Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 43-meric（43） Consistent with all polymers |
Chain K
1–215（215 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6BK8 S. cerevisiae spliceosomal post-catalytic P complex Deposited 2017-11-07 | Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric（46） Consistent with all polymers |
Chain R
1–215（215 aa）
|
Not recorded | MG MAGNESIUM ION × 5 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 6EXN Post-catalytic P complex spliceosome with 3' splice site docked Deposited 2017-11-08 | Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 40-meric（40） Review required |
Chain y
1–215（215 aa）
|
Not recorded | MG MAGNESIUM ION × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；3 uL sample was applied to the grid, left for 30s, then blotted for 3s and immediately plunged into liquid ethane.
|
Resolution 3.70 Å |
| 6J6G Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom Deposited 2019-01-15 | Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric（41） Consistent with all polymers |
Chain I
1–215（215 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6J6H Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom Deposited 2019-01-15 | Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric（41） Consistent with all polymers |
Chain I
1–215（215 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6J6N Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom Deposited 2019-01-15 | Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric（41） Consistent with all polymers |
Chain I
1–215（215 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.86 Å |
| 6J6Q Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom Deposited 2019-01-15 | Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 42-meric（42） Consistent with all polymers |
Chain I
1–215（215 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 9DTR Structure of the yeast post-catalytic P complex spliceosome at 2.3 Angstrom resolution Deposited 2024-10-01 | Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 47-meric（47） Consistent with all polymers |
Chain y
1–215（215 aa）
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 4 IHP INOSITOL HEXAKISPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.31 Å |