Current Protein Identity：P61088
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1J7D Crystal Structure of hMms2-hUbc13 Deposited 2001-05-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；20% PEG 8000, 100mM Citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.85 Å R-free 0.244 |
| 2C2V Crystal structure of the CHIP-UBC13-UEV1a complex Deposited 2005-09-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
2–152（151 aa）
Chain H
2–152（151 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；MCHIP(166-304), UBC13 AND UEV1A WERE COMBINED IN A 1:1:1 MOLAR RATIO, INCUBATED FOR 30 MIN, AND CONCENTRATED TO 10 MG/ML BY ULTRAFILTRATION. CRYSTALS WERE GROWN BY VAPOUR DIFFUSION AT 20C AGAINST 20% (W/V) PEG2000 MME, 100 MM TRIS-HCL (PH 7.0).
|
Resolution 2.90 Å R-free 0.297 |
| 2C2V Crystal structure of the CHIP-UBC13-UEV1a complex Deposited 2005-09-30 | Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain E
2–152（151 aa）
Chain K
2–152（151 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；MCHIP(166-304), UBC13 AND UEV1A WERE COMBINED IN A 1:1:1 MOLAR RATIO, INCUBATED FOR 30 MIN, AND CONCENTRATED TO 10 MG/ML BY ULTRAFILTRATION. CRYSTALS WERE GROWN BY VAPOUR DIFFUSION AT 20C AGAINST 20% (W/V) PEG2000 MME, 100 MM TRIS-HCL (PH 7.0).
|
Resolution 2.90 Å R-free 0.297 |
| 3HCT Crystal structure of TRAF6 in complex with Ubc13 in the P1 space group Deposited 2009-05-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–152（152 aa）
|
Not recorded | ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；8% PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.10 Å R-free 0.278 |
| 3HCU Crystal structure of TRAF6 in complex with Ubc13 in the C2 space group Deposited 2009-05-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–152（152 aa）
Fragment：Ubc13
|
Not recorded | ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；16-20% PEG 4000, 0.2M Magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.333 |
| 3HCU Crystal structure of TRAF6 in complex with Ubc13 in the C2 space group Deposited 2009-05-06 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–152（152 aa）
Fragment：Ubc13
|
Not recorded | ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；16-20% PEG 4000, 0.2M Magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.333 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 10 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain X
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 11 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain Z
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 12 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain b
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 13 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain e
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 14 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain g
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 15 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain i
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 16 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain l
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 17 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain n
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 18 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain p
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 4 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain J
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain L
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain N
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 7 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain Q
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain S
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3VON Crystalstructure of the ubiquitin protease Deposited 2012-01-30 | Assembly 9 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain U
3–150（148 aa）
Fragment：UNP residues 3-150
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；90mM Tris-HCl buffer (pH 8.5), 19% PEG 3350, 10mM EDTA sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.281 |
| 3W31 Structual basis for the recognition of Ubc13 by the Shigella flexneri effector OspI Deposited 2012-12-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–152（152 aa）
|
Not recorded | IOD IODIDE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.4M potassium iodide, 0.1M MES (pH6.5), 4mM DTT, 15% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.96 Å R-free 0.284 |
| 4DHI Structure of C. elegans OTUB1 bound to human UBC13 Deposited 2012-01-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；100 mM sodium cacodylate, 1 M trisodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.209 |
| 4DHJ The structure of a ceOTUB1 ubiquitin aldehyde UBC13~Ub complex Deposited 2012-01-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；23% PEG350, 0.26-0.30 M sodium chloride, 100 mM Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.35 Å R-free 0.237 |
| 4DHJ The structure of a ceOTUB1 ubiquitin aldehyde UBC13~Ub complex Deposited 2012-01-27 | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain G
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；23% PEG350, 0.26-0.30 M sodium chloride, 100 mM Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.35 Å R-free 0.237 |
| 4DHJ The structure of a ceOTUB1 ubiquitin aldehyde UBC13~Ub complex Deposited 2012-01-27 | Assembly 4 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain K
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；23% PEG350, 0.26-0.30 M sodium chloride, 100 mM Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.35 Å R-free 0.237 |
| 4DHJ The structure of a ceOTUB1 ubiquitin aldehyde UBC13~Ub complex Deposited 2012-01-27 | Assembly 5 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain N
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；23% PEG350, 0.26-0.30 M sodium chloride, 100 mM Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.35 Å R-free 0.237 |
| 4DHZ The structure of h/ceOTUB1-ubiquitin aldehyde-UBC13~Ub Deposited 2012-01-30 | Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain F
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；21% PEG10000, 0.1 M sodium chloride, 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.11 Å R-free 0.288 |
| 4IP3 Complex structure of OspI and Ubc13 Deposited 2013-01-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；298 K；0.2 M Sodium thiocyanate, 20% PEG3350, 0.1 M HEPES pH7.0, VAPOR DIFFUSION, temperature 298K
|
Resolution 2.30 Å R-free 0.248 |
| 4NR3 Crystal Structure of a human Mms2/Ubc13 L121G mutant Deposited 2013-11-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
2–150（149 aa）
|
Mutation：L121G | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.8；298 K；20% PEG 8000, 0.1 M sodium citrate, pH 6.8, vapor diffusion, sitting drop, temperature 298K
|
Resolution 1.80 Å R-free 0.234 |
| 4NRG Crystal Structure of a human Mms2/Ubc13 D118G mutant Deposited 2013-11-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–152（152 aa）
|
Mutation：D118G | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.8；298 K；20% PEG 8000, 0.1 M sodium citrate, pH 6.8, vapor diffusion, sitting drop, temperature 298K
|
Resolution 1.95 Å R-free 0.211 |
| 4NRI Crystal Structure of a human Mms2/Ubc13 A122G mutant Deposited 2013-11-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
3–150（148 aa）
|
Mutation：A122G | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.8；298 K；20% PEG 8000, 0.1 M sodium citrate, pH 6.8, vapor diffusion, sitting drop, temperature 298K
|
Resolution 2.30 Å R-free 0.235 |
| 4ONL Crystal structure of human Mms2/Ubc13_D81N, R85S, A122V, N123P Deposited 2014-01-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；298 K；100 mM sodium citrate, 15% PEG 8000, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.35 Å R-free 0.199 |
| 4ONM Crystal structure of human Mms2/Ubc13 - NSC697923 Deposited 2014-01-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–152（152 aa）
|
Not recorded | GOL GLYCEROL × 1 N2F 2-[(4-methylphenyl)sulfonyl]-5-nitrofuran × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；298 K；100 mM sodium citrate, 15% PEG 8000, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.35 Å R-free 0.192 |
| 4ONN Crystal structure of human Mms2/Ubc13 - BAY 11-7082 Deposited 2014-01-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–152（152 aa）
|
Not recorded | GOL GLYCEROL × 3 BY1 3-[(4-methylphenyl)sulfonyl]prop-2-enenitrile × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；298 K；100 mM sodium citrate, 15% PEG 8000, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.50 Å R-free 0.213 |
| 4ORH Crystal structure of RNF8 bound to the UBC13/MMS2 heterodimer Deposited 2014-02-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain J
1–152（152 aa）
|
Not recorded | ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；75 mM Sodium Acetate, 1.0 M Ammonium Phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 4.80 Å R-free 0.282 |
| 4ORH Crystal structure of RNF8 bound to the UBC13/MMS2 heterodimer Deposited 2014-02-11 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain F
1–152（152 aa）
|
Not recorded | ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；75 mM Sodium Acetate, 1.0 M Ammonium Phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 4.80 Å R-free 0.282 |
| 4ORH Crystal structure of RNF8 bound to the UBC13/MMS2 heterodimer Deposited 2014-02-11 | Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–152（152 aa）
|
Not recorded | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；75 mM Sodium Acetate, 1.0 M Ammonium Phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 4.80 Å R-free 0.282 |
| 4TKP Complex of Ubc13 with the RING domain of the TRIM5alpha retroviral restriction factor Deposited 2014-05-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–152（151 aa）
|
Not recorded | SO4 SULFATE ION × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；295 K；2.0 M ammonium sulfate, 0.1 M bis-tris
|
Resolution 2.08 Å R-free 0.255 |
| 4WHV E3 ubiquitin-protein ligase RNF8 in complex with Ubiquitin-conjugating enzyme E2 N and Polyubiquitin-B Deposited 2014-09-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–152（152 aa）
Fragment：unp residues 1-152
Chain E
1–152（152 aa）
Fragment：unp residues 1-152
|
Mutation：C87K Mutation：C87K | ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；1.04 M (NH4)2HPO4
|
Resolution 8.30 Å R-free 0.337 |
| 4WHV E3 ubiquitin-protein ligase RNF8 in complex with Ubiquitin-conjugating enzyme E2 N and Polyubiquitin-B Deposited 2014-09-23 | Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain H
1–152（152 aa）
Fragment：unp residues 1-152
Chain K
1–152（152 aa）
Fragment：unp residues 1-152
|
Mutation：C87K Mutation：C87K | ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；1.04 M (NH4)2HPO4
|
Resolution 8.30 Å R-free 0.337 |
| 5AIT A complex of of RNF4-RING domain, UbeV2, Ubc13-Ub (isopeptide crosslink) Deposited 2015-02-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain B
1–152（152 aa）
Chain E
1–152（152 aa）
|
Mutation：YES Mutation：YES | ZN ZINC ION × 4 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.40 Å R-free 0.286 |
| 5AIU A complex of RNF4-RING domain, Ubc13-Ub (isopeptide crosslink) Deposited 2015-02-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
1–152（152 aa）
Chain E
1–152（152 aa）
|
Mutation：YES Mutation：YES | ZN ZINC ION × 4 EDO 1,2-ETHANEDIOL × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
1,2-ETHANEDIOL
|
Resolution 2.21 Å R-free 0.255 |
| 5EYA TRIM25 RING domain in complex with Ubc13-Ub conjugate Deposited 2015-11-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
1–152（152 aa）
Chain B
1–152（152 aa）
|
Mutation：C87K Mutation：C87K | ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Li citrate, 20% PEG 3350
|
Resolution 2.40 Å R-free 0.228 |
| 5H7S Structural basis of the flanking zinc-finger motifs crucial for the E3 ligase activity of the LNX1 RING domain Deposited 2016-11-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
1–152（152 aa）
Chain C
1–152（152 aa）
|
Mutation：C87K, K92A Mutation：C87K, K92A | ZN ZINC ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.4；298 K；0.04 M Citric acid, 0.06 M BIS-TRIS propane, pH 6.4, 20% w/v polyethylene glycol 3350
|
Resolution 3.49 Å R-free 0.286 |
| 5VNZ Structure of a TRAF6-Ubc13~Ub complex Deposited 2017-05-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–152（152 aa）
|
Not recorded | ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；289 K；0.05-0.3 mM sodium citrate, 100 mM bBis-Tris propane, and 17-23% PEG 3350
|
Resolution 3.41 Å R-free 0.294 |
| 5VNZ Structure of a TRAF6-Ubc13~Ub complex Deposited 2017-05-01 | Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain E
1–152（152 aa）
|
Not recorded | ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；289 K；0.05-0.3 mM sodium citrate, 100 mM bBis-Tris propane, and 17-23% PEG 3350
|
Resolution 3.41 Å R-free 0.294 |
| 5VO0 Structure of a TRAF6-Ubc13~Ub complex Deposited 2017-05-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–152（152 aa）
Chain E
1–152（152 aa）
|
Not recorded | ZN ZINC ION × 8 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；289 K；100-200 mM Na/K tartrate, 11-15% PEG 3350 and 100 mM bis-Tris propane pH 7.5
|
Resolution 3.90 Å R-free 0.299 |
| 5YWR Crystal Structure of RING E3 ligase ZNRF1 in complex with Ube2N (Ubc13) Deposited 2017-11-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–152（152 aa）
|
Not recorded | PGE TRIETHYLENE GLYCOL × 1 FMT FORMIC ACID × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；0.2M Magnesium fromate dihydrate, 20% PEG 3350 (w/v)
|
Resolution 1.47 Å R-free 0.184 |
| 6D6I Ube2V1 in complex with ubiquitin variant Ubv.V1.1 and Ube2N/Ubc13 Deposited 2018-04-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
3–152（150 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.2M LiSO4, 25% PEG3350, 0.1 M Tris-HCl pH 8.5
|
Resolution 2.55 Å R-free 0.296 |
| 6D6I Ube2V1 in complex with ubiquitin variant Ubv.V1.1 and Ube2N/Ubc13 Deposited 2018-04-21 | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain E
3–152（150 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.2M LiSO4, 25% PEG3350, 0.1 M Tris-HCl pH 8.5
|
Resolution 2.55 Å R-free 0.296 |
| 6JKY Crystal structure of MvcA-UBE2N-Ub complex from Legionella pneumophila Deposited 2019-03-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–152（152 aa）
|
Mutation：K94A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；289.15 K；0.2 M Sodium thiocyanate, 20% w/v Polyethylene glycol 3,350
|
Resolution 2.45 Å R-free 0.258 |
| 6JKY Crystal structure of MvcA-UBE2N-Ub complex from Legionella pneumophila Deposited 2019-03-03 | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain E
1–152（152 aa）
|
Mutation：K94A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；289.15 K；0.2 M Sodium thiocyanate, 20% w/v Polyethylene glycol 3,350
|
Resolution 2.45 Å R-free 0.258 |
| 6KFP Crystal structure of MavC ternary complex Deposited 2019-07-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；PEG 3350, lithium chloride
|
Resolution 2.92 Å R-free 0.250 |
| 6KG6 Crystal structure of MavC/UBE2N-Ub complex Deposited 2019-07-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain G
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；PEG 4000, Tris
|
Resolution 2.39 Å R-free 0.245 |
| 6KL4 Crystal structure of MavC-UBE2N-Ub Deposited 2019-07-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–152（152 aa）
|
Mutation：K94A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
COUNTER-DIFFUSION；289.15 K；0.2M Sodium malonate pH 6.0, 20%w/v PEG 3,350
|
Resolution 2.85 Å R-free 0.262 |
| 6LP2 Structure of Lpg2148/UBE2N-Ub complex Deposited 2020-01-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain D
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；PEG 3350, potassium citrate
|
Resolution 2.48 Å R-free 0.233 |
| 6P5B Crystal Structure of MavC in Complex with Ub-UbE2N Deposited 2019-05-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；0.2 M Na Malonate, 25% PEG 3350, 10 mM NiCl2
|
Resolution 2.10 Å R-free 0.232 |
| 6S53 Crystal structure of TRIM21 RING domain in complex with an isopeptide-linked Ube2N~ubiquitin conjugate Deposited 2019-06-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain C
1–152（152 aa）
Chain E
1–152（152 aa）
|
Not recorded | ZN ZINC ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；290 K；Crystals grew in 0.1 M Tris/BICINE pH 8.5, 10.5 % (w/v) PEG3350/PEG 1K/MPD and 0.08 M sodium nitrate/sodium phosphate/ammonium sulfate.
|
Resolution 2.80 Å R-free 0.249 |
| 6S53 Crystal structure of TRIM21 RING domain in complex with an isopeptide-linked Ube2N~ubiquitin conjugate Deposited 2019-06-30 | Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain I
1–152（152 aa）
Chain K
1–152（152 aa）
|
Not recorded | ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；290 K；Crystals grew in 0.1 M Tris/BICINE pH 8.5, 10.5 % (w/v) PEG3350/PEG 1K/MPD and 0.08 M sodium nitrate/sodium phosphate/ammonium sulfate.
|
Resolution 2.80 Å R-free 0.249 |
| 6ULH Structure of MavC in complex with its substrate in R3 spacegroup Deposited 2019-10-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；294 K；0.1 M Sodium Acetate: HCl, pH 4.6
3.5 M Sodium Formate
|
Resolution 1.97 Å R-free 0.227 |
| 6UMP Crystal structure of MavC in complex with substrate mimic in P65 space group Deposited 2019-10-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2 M Sodium formate, 20% w/v Polyethylene glycol 3,350
|
Resolution 2.80 Å R-free 0.304 |
| 6UMS Crystal structure of MavC in complex with its substrate mimic in C222(1) space group Deposited 2019-10-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；0.15 M Potassium bromide, 30% (w/v) PEG 2000 MME
|
Resolution 2.34 Å R-free 0.250 |
| 7BBD Crystal structure of monoubiquitinated TRIM21 RING (Ub-RING) In complex with ubiquitin charged Ube2N (Ube2N~Ub) and Ube2V2 Deposited 2020-12-17 | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain C
1–152（152 aa）
|
Mutation：C87K, K92A | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；290.15 K；MOPSO, Bis-Tris, PEG 4K, 1,2,6-hexanetriol, Li, Na, K
|
Resolution 2.20 Å R-free 0.252 |
| 7BBF Crystal structure of ubiquitin charged Ube2N (Ube2N~Ub) in complex with Ube2V2 Deposited 2020-12-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric（9） Consistent with protein count |
Chain A
1–152（152 aa）
Chain D
1–152（152 aa）
Chain G
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；290 K；0.1 M bicine Trizma base pH 8.5, 12.5% PEG 1000, 12.5% PEG 3350, 12.5% MPD, 0.2 % of each Anesthetic alkaloids (lidocaine HCl H2O, procaine HCl, proparacaine HCl, tetracaine HCl)
|
Resolution 2.54 Å R-free 0.253 |
| 7BXG MavC-UBE2N-Ub complex Deposited 2020-04-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；0.1 M sodium malonate, pH 7.0, 12% PEG3350
|
Resolution 2.71 Å R-free 0.243 |
| 9BIV Crystal Structure of Ubc13 with a New Active Site Loop Conformation Deposited 2024-04-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–152（152 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
BATCH MODE；pH 7.5；298 K；Ubc13-Mms2 heterodimer was concentrated to 12 mg/mL and stored in 50 mM Tris pH 7.5, 150 mM NaCl, 1mM TCEP. Complex was left to slowly equilibrate from 277K to 298 overnight
|
Resolution 1.68 Å R-free 0.177 |
| 9LHJ UBE2N/UBE2V2 complexed with a covalent inhibitor Deposited 2025-01-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–152（152 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；289.15 K；0.1 M BIS-TRIS pH 6.5, 20% w/v PEG 5,000
|
Resolution 1.68 Å R-free 0.228 |
| 9LHJ UBE2N/UBE2V2 complexed with a covalent inhibitor Deposited 2025-01-12 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–152（152 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；289.15 K；0.1 M BIS-TRIS pH 6.5, 20% w/v PEG 5,000
|
Resolution 1.68 Å R-free 0.228 |
| 9N1F Crystal Structure of the Ark2C-Ubc13~Ub-Mms2 complex Deposited 2025-01-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
1–152（152 aa）
|
Mutation：C87K, K92T, K94Q | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；289.15 K；0.1 M Sodium Malonate and 18% w/v PEG 3350
|
Resolution 2.10 Å R-free 0.279 |
| 9UV6 Encounter complex structure of E2N and Ubiquitin Deposited 2025-05-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
3–152（150 aa）
|
Mutation：N123C | A1EQF (5~{S},16~{S})-16-[2-[2,5-bis(oxidanylidene)pyrrolidin-1-yl]ethylamino]-2,11,12,17-tetraoxa-5,8-diaza-1$l^{4}-manganatricyclo[6.3.3.3^{1,5}]heptadecane-3,10,13-trione × 1 | SOLUTION NMR |
NMR measurement conditions
pH 7.5；298 K；Ionic strength (raw mmCIF value) 0.02；Pressure 1
NMR sample composition
0.6 mM [U-15N] Ubi, 0.2 mM E2N, 20 mM HEPES, 90 % H2O, 10 % [U-2H] D2O, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |