Current Protein Identity：P80340
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 10PX Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with benzoxaborole derivative of azithromycin (AZI-BB2), mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.45A resolution Deposited 2026-02-01 | Assembly 1 Protein–RNA Heteromer；Protein × 98 PDB declaration: 112-meric（112） Consistent with all polymers |
Chain 17
1–49（49 aa）
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 2794 K POTASSIUM ION × 2 A1C9N AZI-BB2 × 2 ZN ZINC ION × 12 SF4 IRON/SULFUR CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.45 Å R-free 0.300 |
| 1VVJ Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome Deposited 2013-05-24 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 286 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
|
Resolution 3.44 Å R-free 0.285 |
| 1VVJ Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome Deposited 2013-05-24 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 315 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
|
Resolution 3.44 Å R-free 0.285 |
| 1VY4 Crystal structure of the Thermus thermophilus 70S ribosome in the pre-attack state of peptide bond formation containing acylated tRNA-substrates in the A and P sites. Deposited 2014-05-13 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1159 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.60 Å R-free 0.264 |
| 1VY4 Crystal structure of the Thermus thermophilus 70S ribosome in the pre-attack state of peptide bond formation containing acylated tRNA-substrates in the A and P sites. Deposited 2014-05-13 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 889 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.60 Å R-free 0.264 |
| 1VY5 Crystal structure of the Thermus thermophilus 70S ribosome in the post-catalysis state of peptide bond formation containing dipeptydil-tRNA in the A site and deacylated tRNA in the P site. Deposited 2014-05-13 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1165 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.55 Å R-free 0.280 |
| 1VY5 Crystal structure of the Thermus thermophilus 70S ribosome in the post-catalysis state of peptide bond formation containing dipeptydil-tRNA in the A site and deacylated tRNA in the P site. Deposited 2014-05-13 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 912 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.55 Å R-free 0.280 |
| 1VY6 Crystal structure of the Thermus thermophilus 70S ribosome in the pre-attack state of peptide bond formation containing short substrate-mimic Cytidine-Puromycin in the A site and acylated tRNA in the P site. Deposited 2014-05-13 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 965 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.90 Å R-free 0.286 |
| 1VY6 Crystal structure of the Thermus thermophilus 70S ribosome in the pre-attack state of peptide bond formation containing short substrate-mimic Cytidine-Puromycin in the A site and acylated tRNA in the P site. Deposited 2014-05-13 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 799 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.90 Å R-free 0.286 |
| 1VY7 Crystal structure of the Thermus thermophilus 70S ribosome in the pre-attack state of peptide bond formation containing short substrate-mimic Cytidine-Cytidine-Puromycin in the A site and acylated tRNA in the P site. Deposited 2014-05-13 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1055 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.80 Å R-free 0.280 |
| 1VY7 Crystal structure of the Thermus thermophilus 70S ribosome in the pre-attack state of peptide bond formation containing short substrate-mimic Cytidine-Cytidine-Puromycin in the A site and acylated tRNA in the P site. Deposited 2014-05-13 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 845 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.80 Å R-free 0.280 |
| 4L47 Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U on the Ribosome Deposited 2013-06-07 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 325 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
|
Resolution 3.22 Å R-free 0.260 |
| 4L47 Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U on the Ribosome Deposited 2013-06-07 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 354 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
|
Resolution 3.22 Å R-free 0.260 |
| 4L71 Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-A on the Ribosome Deposited 2013-06-13 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 325 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
|
Resolution 3.90 Å R-free 0.278 |
| 4L71 Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-A on the Ribosome Deposited 2013-06-13 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 351 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
|
Resolution 3.90 Å R-free 0.278 |
| 4LEL Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCG-G on the Ribosome Deposited 2013-06-25 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 321 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
|
Resolution 3.90 Å R-free 0.298 |
| 4LEL Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCG-G on the Ribosome Deposited 2013-06-25 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 349 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
|
Resolution 3.90 Å R-free 0.298 |
| 4LFZ Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U in the Absence of Paromomycin Deposited 2013-06-27 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 320 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, vapor diffusion, temperature 293K
|
Resolution 3.92 Å R-free 0.265 |
| 4LFZ Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U in the Absence of Paromomycin Deposited 2013-06-27 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 354 ZN ZINC ION × 3 AMP ADENOSINE MONOPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, vapor diffusion, temperature 293K
|
Resolution 3.92 Å R-free 0.265 |
| 4LNT Crystal Structure of tRNA Proline (CGG) Bound to Codon CCC-U on the Ribosome Deposited 2013-07-12 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 325 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, vapor diffusion, temperature 293K
|
Resolution 2.94 Å R-free 0.265 |
| 4LNT Crystal Structure of tRNA Proline (CGG) Bound to Codon CCC-U on the Ribosome Deposited 2013-07-12 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 352 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, vapor diffusion, temperature 293K
|
Resolution 2.94 Å R-free 0.265 |
| 4LSK Crystal Structure of tRNA Proline (CGG) Bound to Codon CCG-G on the Ribosome Deposited 2013-07-22 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 321 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
|
Resolution 3.48 Å R-free 0.270 |
| 4LSK Crystal Structure of tRNA Proline (CGG) Bound to Codon CCG-G on the Ribosome Deposited 2013-07-22 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 351 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
|
Resolution 3.48 Å R-free 0.270 |
| 4LT8 Crystal Structure of tRNA Proline (CGG) Bound to Codon CCC-G on the Ribosome Deposited 2013-07-23 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 324 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, vapor diffusion, temperature 293K
|
Resolution 3.14 Å R-free 0.262 |
| 4LT8 Crystal Structure of tRNA Proline (CGG) Bound to Codon CCC-G on the Ribosome Deposited 2013-07-23 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 352 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, vapor diffusion, temperature 293K
|
Resolution 3.14 Å R-free 0.262 |
| 4P6F Crystal structure of the peptolide 12C bound to bacterial ribosome Deposited 2014-03-24 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 328 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M Tris-Acetate, 0.2M KSCN, 10 mM MgCl2
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M Tris-Acetate, 0.2M KSCN, 10 mM MgCl2
|
Resolution 3.60 Å R-free 0.254 |
| 4P6F Crystal structure of the peptolide 12C bound to bacterial ribosome Deposited 2014-03-24 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 368 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M Tris-Acetate, 0.2M KSCN, 10 mM MgCl2
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M Tris-Acetate, 0.2M KSCN, 10 mM MgCl2
|
Resolution 3.60 Å R-free 0.254 |
| 4P70 Crystal Structure of Unmodified tRNA Proline (CGG) Bound to Codon CCG on the Ribosome Deposited 2014-03-25 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 338 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
|
Resolution 3.68 Å R-free 0.272 |
| 4P70 Crystal Structure of Unmodified tRNA Proline (CGG) Bound to Codon CCG on the Ribosome Deposited 2014-03-25 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 362 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
|
Resolution 3.68 Å R-free 0.272 |
| 4TUA Crystal structure of ASL-Thr bound to Codon ACC-A on the Ribosome Deposited 2014-06-24 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 425 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
|
Resolution 3.60 Å R-free 0.252 |
| 4TUA Crystal structure of ASL-Thr bound to Codon ACC-A on the Ribosome Deposited 2014-06-24 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 471 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
|
Resolution 3.60 Å R-free 0.252 |
| 4TUB Crystal structure of tRNA-Thr bound to Codon ACC-C on the Ribosome Deposited 2014-06-24 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 426 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
|
Resolution 3.60 Å R-free 0.259 |
| 4TUB Crystal structure of tRNA-Thr bound to Codon ACC-C on the Ribosome Deposited 2014-06-24 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 492 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
|
Resolution 3.60 Å R-free 0.259 |
| 4TUC Crystal structure of ASL-SufJ bound to Codon ACC-A on the Ribosome Deposited 2014-06-24 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 354 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
|
Resolution 3.60 Å R-free 0.253 |
| 4TUC Crystal structure of ASL-SufJ bound to Codon ACC-A on the Ribosome Deposited 2014-06-24 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 394 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
|
Resolution 3.60 Å R-free 0.253 |
| 4TUD Crystal structure of ASL-SufJ bound to Codon ACC-C on the Ribosome Deposited 2014-06-24 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 393 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
|
Resolution 3.60 Å R-free 0.244 |
| 4TUD Crystal structure of ASL-SufJ bound to Codon ACC-C on the Ribosome Deposited 2014-06-24 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 418 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
|
Resolution 3.60 Å R-free 0.244 |
| 4TUE Crystal structure of ASL-SufJ bound to Codon ACC-U on the Ribosome Deposited 2014-06-24 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 422 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
|
Resolution 3.50 Å R-free 0.250 |
| 4TUE Crystal structure of ASL-SufJ bound to Codon ACC-U on the Ribosome Deposited 2014-06-24 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 463 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
|
Resolution 3.50 Å R-free 0.250 |
| 4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain Z
1–49（49 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.71 Å R-free 0.353 |
| 4V4J Interactions and Dynamics of the Shine-Dalgarno Helix in the 70S Ribosome. Deposited 2007-07-18 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain Z
1–49（49 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K
|
Resolution 3.83 Å R-free 0.351 |
| 4V4X Crystal structure of the 70S Thermus thermophilus ribosome showing how the 16S 3'-end mimicks mRNA E and P codons. Deposited 2006-06-27 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain B6
1–49（49 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 5.00 Å R-free 0.323 |
| 4V4Y Crystal structure of the 70S Thermus thermophilus ribosome with translocated and rotated Shine-Dalgarno Duplex. Deposited 2006-06-27 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain B6
1–49（49 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 5.50 Å R-free 0.326 |
| 4V4Z 70S Thermus thermophilous ribosome functional complex with mRNA and E- and P-site tRNAs at 4.5A. Deposited 2006-06-27 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain B6
1–49（49 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 4.51 Å R-free 0.345 |
| 4V51 Structure of the Thermus thermophilus 70S ribosome complexed with mRNA, tRNA and paromomycin Deposited 2006-07-31 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 741 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7；0.2M KSCN, 4% PEG 20K, 4% PEG550 MME, 0.1M TRIS-ACETATE PH 7
|
Resolution 2.80 Å R-free 0.313 |
| 4V51 Structure of the Thermus thermophilus 70S ribosome complexed with mRNA, tRNA and paromomycin Deposited 2006-07-31 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 638 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7；0.2M KSCN, 4% PEG 20K, 4% PEG550 MME, 0.1M TRIS-ACETATE PH 7
|
Resolution 2.80 Å R-free 0.313 |
| 4V5A Structure of the Ribosome Recycling Factor bound to the Thermus thermophilus 70S ribosome with mRNA, ASL-Phe and tRNA-fMet Deposited 2007-06-28 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 741 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7；COMPARE SELMER ET AL SCIENCE 2006, pH 7.0
|
Resolution 3.50 Å R-free 0.326 |
| 4V5A Structure of the Ribosome Recycling Factor bound to the Thermus thermophilus 70S ribosome with mRNA, ASL-Phe and tRNA-fMet Deposited 2007-06-28 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 741 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7；COMPARE SELMER ET AL SCIENCE 2006, pH 7.0
|
Resolution 3.50 Å R-free 0.326 |
| 4V5C Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A-site tRNA, deacylated P-site tRNA, and E-site tRNA. Deposited 2009-03-24 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 741 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；pH 7.1
|
Resolution 3.30 Å R-free 0.272 |
| 4V5C Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A-site tRNA, deacylated P-site tRNA, and E-site tRNA. Deposited 2009-03-24 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 741 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；pH 7.1
|
Resolution 3.30 Å R-free 0.272 |
| 4V5D Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A- and P-site tRNAs, and E-site tRNA. Deposited 2009-03-24 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 673 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；pH 7.1
|
Resolution 3.50 Å R-free 0.256 |
| 4V5D Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A- and P-site tRNAs, and E-site tRNA. Deposited 2009-03-24 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 673 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；pH 7.1
|
Resolution 3.50 Å R-free 0.256 |
| 4V5E Insights into translational termination from the structure of RF2 bound to the ribosome Deposited 2009-04-30 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 546 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；SEE SELMER ET AL., SCIENCE 2006, pH 7.1
|
Resolution 3.45 Å R-free 0.257 |
| 4V5E Insights into translational termination from the structure of RF2 bound to the ribosome Deposited 2009-04-30 | Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 546 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；SEE SELMER ET AL., SCIENCE 2006, pH 7.1
|
Resolution 3.45 Å R-free 0.257 |
| 4V5F The structure of the ribosome with elongation factor G trapped in the post-translocational state Deposited 2009-09-01 | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 62-meric（62） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；100 MM MES PH 6.5, 12%PEG 20K, 50 MM KCL, 10 MM NH4CL, 3UM DEOXY-BIG-CHAP
|
Resolution 3.60 Å R-free 0.260 |
| 4V5F The structure of the ribosome with elongation factor G trapped in the post-translocational state Deposited 2009-09-01 | Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 62-meric（62） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；100 MM MES PH 6.5, 12%PEG 20K, 50 MM KCL, 10 MM NH4CL, 3UM DEOXY-BIG-CHAP
|
Resolution 3.60 Å R-free 0.260 |
| 4V5G The crystal structure of the 70S ribosome bound to EF-Tu and tRNA Deposited 2009-09-01 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 ZN ZINC ION × 4 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 22-25 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.4% (W/V) PEG20K
|
Resolution 3.60 Å R-free 0.315 |
| 4V5G The crystal structure of the 70S ribosome bound to EF-Tu and tRNA Deposited 2009-09-01 | Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 ZN ZINC ION × 4 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 22-25 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.4% (W/V) PEG20K
|
Resolution 3.60 Å R-free 0.315 |
| 4V5J Structure of the 70S ribosome bound to Release factor 2 and a substrate analog provides insights into catalysis of peptide release Deposited 2010-03-24 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 548 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；SEE SELMER ET AL., SCIENCE 2006, pH 6.5
|
Resolution 3.10 Å R-free 0.264 |
| 4V5J Structure of the 70S ribosome bound to Release factor 2 and a substrate analog provides insights into catalysis of peptide release Deposited 2010-03-24 | Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 546 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；SEE SELMER ET AL., SCIENCE 2006, pH 6.5
|
Resolution 3.10 Å R-free 0.264 |
| 4V5K Structure of cytotoxic domain of colicin E3 bound to the 70S ribosome Deposited 2010-05-29 | Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1164 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.18；AS STATED IN STRUCTURE OF THE 70S RIBOSOME COMPLEXED WITH MRNA AND TRNA. (2006) SCIENCE 313: 1935, PH 7.18
|
Resolution 3.20 Å R-free 0.270 |
| 4V5K Structure of cytotoxic domain of colicin E3 bound to the 70S ribosome Deposited 2010-05-29 | Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1170 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.18；AS STATED IN STRUCTURE OF THE 70S RIBOSOME COMPLEXED WITH MRNA AND TRNA. (2006) SCIENCE 313: 1935, PH 7.18
|
Resolution 3.20 Å R-free 0.270 |
| 4V5L The structure of EF-Tu and aminoacyl-tRNA bound to the 70S ribosome with a GTP analog Deposited 2010-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, AND 5.3% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.268 |
| 4V5M tRNA tranlocation on the 70S ribosome: the pre-translocational translocation intermediate TI(PRE) Deposited 2010-10-01 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- VITROBOT (FEI)
|
Resolution 7.80 Å |
| 4V5N tRNA translocation on the 70S ribosome: the post- translocational translocation intermediate TI(POST) Deposited 2010-10-21 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- VITROBOT (FEI)
|
Resolution 7.60 Å |
| 4V5P The crystal structure of EF-Tu and A9C-tRNA-Trp bound to a near- cognate codon on the 70S ribosome Deposited 2010-12-07 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.267 |
| 4V5P The crystal structure of EF-Tu and A9C-tRNA-Trp bound to a near- cognate codon on the 70S ribosome Deposited 2010-12-07 | Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.267 |
| 4V5Q The crystal structure of EF-Tu and G24A-tRNA-Trp bound to a near- cognate codon on the 70S ribosome Deposited 2010-12-07 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.275 |
| 4V5Q The crystal structure of EF-Tu and G24A-tRNA-Trp bound to a near- cognate codon on the 70S ribosome Deposited 2010-12-07 | Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.275 |
| 4V5R The crystal structure of EF-Tu and Trp-tRNA-Trp bound to a cognate codon on the 70S ribosome. Deposited 2010-12-07 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.264 |
| 4V5R The crystal structure of EF-Tu and Trp-tRNA-Trp bound to a cognate codon on the 70S ribosome. Deposited 2010-12-07 | Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.264 |
| 4V5S The crystal structure of EF-Tu and G24A-tRNA-Trp bound to a cognate codon on the 70S ribosome. Deposited 2010-12-07 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.285 |
| 4V5S The crystal structure of EF-Tu and G24A-tRNA-Trp bound to a cognate codon on the 70S ribosome. Deposited 2010-12-07 | Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.285 |
| 4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 3.21 Å R-free 0.319 |
| 4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 3.21 Å R-free 0.319 |
| 4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 3.00 Å R-free 0.316 |
| 4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 801 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 3.00 Å R-free 0.316 |
| 4V68 T. thermophilus 70S ribosome in complex with mRNA, tRNAs and EF-Tu.GDP.kirromycin ternary complex, fitted to a 6.4 A Cryo-EM map. Deposited 2008-12-11 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | PHA PHENYLALANINAL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MAU N-METHYL KIRROMYCIN × 1 BME BETA-MERCAPTOETHANOL × 1 | ELECTRON MICROSCOPY |
cryo-EM vitrification conditions
Cryogen METHANE；A Vitrobot was used to shock-freeze the sample in liquid methane.
|
Resolution 6.40 Å |
| 4V6A Structure of EF-P bound to the 70S ribosome. Deposited 2009-06-15 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 611 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5 % Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
|
Resolution 3.10 Å R-free 0.302 |
| 4V6A Structure of EF-P bound to the 70S ribosome. Deposited 2009-06-15 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 439 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5 % Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
|
Resolution 3.10 Å R-free 0.302 |
| 4V6F Elongation complex of the 70S ribosome with three tRNAs and mRNA. Deposited 2009-07-09 | Assembly 1 Insufficient information Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain A7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 2089 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, temperature 293K
|
Resolution 3.10 Å R-free 0.255 |
| 4V6F Elongation complex of the 70S ribosome with three tRNAs and mRNA. Deposited 2009-07-09 | Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 3256 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, temperature 293K
|
Resolution 3.10 Å R-free 0.255 |
| 4V6G Initiation complex of 70S ribosome with two tRNAs and mRNA. Deposited 2009-07-10 | Assembly 1 Insufficient information Heteromer；Protein × 48 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1216 ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, temperature 293K
|
Resolution 3.50 Å R-free 0.252 |
| 4V6G Initiation complex of 70S ribosome with two tRNAs and mRNA. Deposited 2009-07-10 | Assembly 2 Insufficient information Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1437 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, temperature 293K
|
Resolution 3.50 Å R-free 0.252 |
| 4V7J Structure of RelE nuclease bound to the 70S ribosome (precleavage state) Deposited 2009-11-02 | Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain A7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 532 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；0.1M Tris-HAc, 0.2M KSCN, 3-4.5% w/v PEG 20k, 3-4.5% PEG 550 monomethylether, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.30 Å R-free 0.247 |
| 4V7J Structure of RelE nuclease bound to the 70S ribosome (precleavage state) Deposited 2009-11-02 | Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 532 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；0.1M Tris-HAc, 0.2M KSCN, 3-4.5% w/v PEG 20k, 3-4.5% PEG 550 monomethylether, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.30 Å R-free 0.247 |
| 4V7K Structure of RelE nuclease bound to the 70S ribosome (postcleavage state) Deposited 2009-11-02 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain A7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 532 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；0.1 M Tris-HAc, 0.2 M KSCN, 3-4.5% PEG20K (W/V), 3-4.5% PEG550 MME, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.60 Å R-free 0.245 |
| 4V7K Structure of RelE nuclease bound to the 70S ribosome (postcleavage state) Deposited 2009-11-02 | Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 532 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；0.1 M Tris-HAc, 0.2 M KSCN, 3-4.5% PEG20K (W/V), 3-4.5% PEG550 MME, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.60 Å R-free 0.245 |
| 4V7L The structures of viomycin bound to the 70S ribosome. Deposited 2009-11-12 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 577 ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5% Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
|
Resolution 3.00 Å R-free 0.272 |
| 4V7L The structures of viomycin bound to the 70S ribosome. Deposited 2009-11-12 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 388 ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5% Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
|
Resolution 3.00 Å R-free 0.272 |
| 4V7M The structures of Capreomycin bound to the 70S ribosome. Deposited 2009-11-12 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 483 ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5% Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
|
Resolution 3.45 Å R-free 0.268 |
| 4V7M The structures of Capreomycin bound to the 70S ribosome. Deposited 2009-11-12 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 359 ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5% Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
|
Resolution 3.45 Å R-free 0.268 |
| 4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain B4
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 250 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
|
Resolution 3.62 Å R-free 0.291 |
| 4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain C4
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 160 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
|
Resolution 3.62 Å R-free 0.291 |
| 4V7W Structure of the Thermus thermophilus ribosome complexed with chloramphenicol. Deposited 2010-08-16 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 445 ZN ZINC ION × 2 K POTASSIUM ION × 1 CLM CHLORAMPHENICOL × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.00 Å R-free 0.281 |
| 4V7W Structure of the Thermus thermophilus ribosome complexed with chloramphenicol. Deposited 2010-08-16 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 400 ZN ZINC ION × 2 K POTASSIUM ION × 1 CLM CHLORAMPHENICOL × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.00 Å R-free 0.281 |
| 4V7X Structure of the Thermus thermophilus ribosome complexed with erythromycin. Deposited 2010-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 438 ZN ZINC ION × 2 K POTASSIUM ION × 1 ERY ERYTHROMYCIN A × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.00 Å R-free 0.278 |
| 4V7X Structure of the Thermus thermophilus ribosome complexed with erythromycin. Deposited 2010-08-17 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 392 ZN ZINC ION × 2 K POTASSIUM ION × 1 ERY ERYTHROMYCIN A × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.00 Å R-free 0.278 |
| 4V7Y Structure of the Thermus thermophilus 70S ribosome complexed with azithromycin. Deposited 2010-08-18 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 421 ZN ZINC ION × 2 K POTASSIUM ION × 1 ZIT AZITHROMYCIN × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.00 Å R-free 0.269 |
| 4V7Y Structure of the Thermus thermophilus 70S ribosome complexed with azithromycin. Deposited 2010-08-18 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 373 ZN ZINC ION × 2 K POTASSIUM ION × 1 ZIT AZITHROMYCIN × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.00 Å R-free 0.269 |
| 4V7Z Structure of the Thermus thermophilus 70S ribosome complexed with telithromycin. Deposited 2010-08-18 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 435 ZN ZINC ION × 2 K POTASSIUM ION × 1 TEL TELITHROMYCIN × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.10 Å R-free 0.284 |
| 4V7Z Structure of the Thermus thermophilus 70S ribosome complexed with telithromycin. Deposited 2010-08-18 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 384 ZN ZINC ION × 2 K POTASSIUM ION × 1 TEL TELITHROMYCIN × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.10 Å R-free 0.284 |
| 4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain B4
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
|
Resolution 3.50 Å R-free 0.264 |
| 4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain D4
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
|
Resolution 3.50 Å R-free 0.264 |
| 4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain B4
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 595 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
|
Resolution 3.40 Å R-free 0.266 |
| 4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain D4
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 599 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
|
Resolution 3.40 Å R-free 0.266 |
| 4V87 Crystal structure analysis of ribosomal decoding. Deposited 2011-09-20 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain A7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 937 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.10 Å R-free 0.240 |
| 4V87 Crystal structure analysis of ribosomal decoding. Deposited 2011-09-20 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 779 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.10 Å R-free 0.240 |
| 4V8A The structure of thermorubin in complex with the 70S ribosome from Thermus thermophilus. Deposited 2011-12-05 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain A7
1–49（49 aa）
|
Not recorded | T8B Thermorubin × 1 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；2.9% PEG 20K, 9% MPD, 0.1M Tris-HCl pH 7.6, 175 mM L-Arginine, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.20 Å R-free 0.273 |
| 4V8A The structure of thermorubin in complex with the 70S ribosome from Thermus thermophilus. Deposited 2011-12-05 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | T8B Thermorubin × 1 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；2.9% PEG 20K, 9% MPD, 0.1M Tris-HCl pH 7.6, 175 mM L-Arginine, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.20 Å R-free 0.273 |
| 4V8B Crystal structure analysis of ribosomal decoding (near-cognate tRNA-leu complex). Deposited 2011-12-06 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 924 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.00 Å R-free 0.272 |
| 4V8B Crystal structure analysis of ribosomal decoding (near-cognate tRNA-leu complex). Deposited 2011-12-06 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 772 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.00 Å R-free 0.272 |
| 4V8C Crystal structure analysis of ribosomal decoding (near-cognate tRNA-leu complex with paromomycin). Deposited 2011-12-07 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain A7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 926 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.30 Å R-free 0.237 |
| 4V8C Crystal structure analysis of ribosomal decoding (near-cognate tRNA-leu complex with paromomycin). Deposited 2011-12-07 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 771 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.30 Å R-free 0.237 |
| 4V8D Structure analysis of ribosomal decoding (cognate tRNA-tyr complex). Deposited 2011-12-07 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 847 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.00 Å R-free 0.235 |
| 4V8D Structure analysis of ribosomal decoding (cognate tRNA-tyr complex). Deposited 2011-12-07 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 757 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.00 Å R-free 0.235 |
| 4V8E Crystal structure analysis of ribosomal decoding (near-cognate tRNA-tyr complex). Deposited 2011-12-07 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain A7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 479 OHX osmium (III) hexammine × 370 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.30 Å R-free 0.259 |
| 4V8E Crystal structure analysis of ribosomal decoding (near-cognate tRNA-tyr complex). Deposited 2011-12-07 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain C7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 415 OHX osmium (III) hexammine × 342 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.30 Å R-free 0.259 |
| 4V8F Crystal structure analysis of ribosomal decoding (near-cognate tRNA-ttyr complex with paromomycin). Deposited 2011-12-07 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain A7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 486 OHX osmium (III) hexammine × 369 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.30 Å R-free 0.247 |
| 4V8F Crystal structure analysis of ribosomal decoding (near-cognate tRNA-ttyr complex with paromomycin). Deposited 2011-12-07 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 417 OHX osmium (III) hexammine × 341 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.30 Å R-free 0.247 |
| 4V8G Crystal structure of RMF bound to the 70S ribosome. Deposited 2011-12-11 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 776 ZN ZINC ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 3.00 Å R-free 0.254 |
| 4V8G Crystal structure of RMF bound to the 70S ribosome. Deposited 2011-12-11 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 512 ZN ZINC ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 3.00 Å R-free 0.254 |
| 4V8H Crystal structure of HPF bound to the 70S ribosome. Deposited 2011-12-11 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 857 ZN ZINC ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 3.10 Å R-free 0.258 |
| 4V8H Crystal structure of HPF bound to the 70S ribosome. Deposited 2011-12-11 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 791 ZN ZINC ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 3.10 Å R-free 0.258 |
| 4V8I Crystal structure of YfiA bound to the 70S ribosome. Deposited 2011-12-12 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1042 ZN ZINC ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.70 Å R-free 0.254 |
| 4V8I Crystal structure of YfiA bound to the 70S ribosome. Deposited 2011-12-12 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 889 ZN ZINC ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.70 Å R-free 0.254 |
| 4V8J Crystal structure of the bacterial ribosome ram mutation G347U. Deposited 2011-12-20 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 369 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10MM MGCL2, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K
|
Resolution 3.90 Å R-free 0.269 |
| 4V8J Crystal structure of the bacterial ribosome ram mutation G347U. Deposited 2011-12-20 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 371 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10MM MGCL2, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K
|
Resolution 3.90 Å R-free 0.269 |
| 4V8N The crystal structure of agmatidine tRNA-Ile2 bound to the 70S ribosome in the A and P site. Deposited 2013-02-13 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；0.1 M TRIS-ACETATE PH 7, 0.2 M KSCN, 3.5-5.5% (W/V) PEG 20K, AND 3.5-5.5% (W/V) PEG 550 MONOMETHYL ETHER
|
Resolution 3.10 Å R-free 0.280 |
| 4V8N The crystal structure of agmatidine tRNA-Ile2 bound to the 70S ribosome in the A and P site. Deposited 2013-02-13 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；0.1 M TRIS-ACETATE PH 7, 0.2 M KSCN, 3.5-5.5% (W/V) PEG 20K, AND 3.5-5.5% (W/V) PEG 550 MONOMETHYL ETHER
|
Resolution 3.10 Å R-free 0.280 |
| 4V8O Crystal structure of the hybrid state of ribosome in complex with the guanosine triphosphatase release factor 3 Deposited 2011-07-26 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.7；100 MM MES-KOH PH 6.7, 20 MM KCL, 7.4% (W/V) PEG20K
|
Resolution 3.80 Å R-free 0.351 |
| 4V8Q Complex of SmpB, a tmRNA fragment and EF-Tu-GDP-Kirromycin with the 70S ribosome Deposited 2011-12-10 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain A7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 3 MG MAGNESIUM ION × 2 KIR KIRROMYCIN × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；100 MM MES PH 6.5, 20 MM KCL, 8% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.270 |
| 4V8U Crystal Structure of 70S Ribosome with Both Cognate tRNAs in the E and P Sites Representing an Authentic Elongation Complex. Deposited 2012-08-28 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 4 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.4；0.1M MES, PH6.5, 8.5-9.0% PEG20K, 0-25MM KCL, pH 6.4
|
Resolution 3.70 Å R-free 0.249 |
| 4V8U Crystal Structure of 70S Ribosome with Both Cognate tRNAs in the E and P Sites Representing an Authentic Elongation Complex. Deposited 2012-08-28 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | ZN ZINC ION × 4 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.4；0.1M MES, PH6.5, 8.5-9.0% PEG20K, 0-25MM KCL, pH 6.4
|
Resolution 3.70 Å R-free 0.249 |
| 4V8X Structure of Thermus thermophilus ribosome Deposited 2013-07-19 | Assembly 1 Insufficient information Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 352 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；0.1 M TRIS-HAC PH 7.2, 0.2 M KSCN, 4.1%-4.3% (W/V) PEG 20K AND 4.1%-4.3% (W/V) PEG 550MME
|
Resolution 3.35 Å R-free 0.261 |
| 4V8X Structure of Thermus thermophilus ribosome Deposited 2013-07-19 | Assembly 2 Insufficient information Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 355 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；0.1 M TRIS-HAC PH 7.2, 0.2 M KSCN, 4.1%-4.3% (W/V) PEG 20K AND 4.1%-4.3% (W/V) PEG 550MME
|
Resolution 3.35 Å R-free 0.261 |
| 4V90 Thermus thermophilus Ribosome Deposited 2014-02-22 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain B7
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 523 ZN ZINC ION × 3 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；0.1M MES PH 6.5, 5.8-6.8%PEG20K, 30-120 MM KCL AND 0.8-2.5% GLYCEROL
|
Resolution 2.95 Å R-free 0.244 |
| 4V95 Crystal structure of YAEJ bound to the 70S ribosome Deposited 2012-01-27 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1359 ZN ZINC ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, vapor diffusion, temperature 292K
|
Resolution 3.20 Å R-free 0.245 |
| 4V95 Crystal structure of YAEJ bound to the 70S ribosome Deposited 2012-01-27 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 977 ZN ZINC ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, vapor diffusion, temperature 292K
|
Resolution 3.20 Å R-free 0.245 |
| 4V97 Crystal structure of the bacterial ribosome ram mutation G299A. Deposited 2012-04-06 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 457 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.52 Å R-free 0.249 |
| 4V97 Crystal structure of the bacterial ribosome ram mutation G299A. Deposited 2012-04-06 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 576 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.52 Å R-free 0.249 |
| 4V9A Crystal Structure of the 70S ribosome with tetracycline. Deposited 2012-07-18 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 921 TAC TETRACYCLINE × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.30 Å R-free 0.254 |
| 4V9A Crystal Structure of the 70S ribosome with tetracycline. Deposited 2012-07-18 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 766 TAC TETRACYCLINE × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.30 Å R-free 0.254 |
| 4V9B Crystal Structure of the 70S ribosome with tigecycline. Deposited 2012-07-18 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 922 T1C TIGECYCLINE × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.10 Å R-free 0.269 |
| 4V9B Crystal Structure of the 70S ribosome with tigecycline. Deposited 2012-07-18 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 764 T1C TIGECYCLINE × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.10 Å R-free 0.269 |
| 4V9H Crystal structure of the ribosome bound to elongation factor G in the guanosine triphosphatase state Deposited 2013-03-25 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 138 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.3；294 K；100mM MES pH 6.3, 75mM KCl, 6.0% PEG 20K, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 2.86 Å R-free 0.250 |
| 4V9I Crystal structure of thermus thermophilus 70S in complex with tRNAs and mRNA containing a pseudouridine in a stop codon Deposited 2013-04-04 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain B7
1–48（48 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.30 Å R-free 0.279 |
| 4V9I Crystal structure of thermus thermophilus 70S in complex with tRNAs and mRNA containing a pseudouridine in a stop codon Deposited 2013-04-04 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain D7
1–48（48 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.30 Å R-free 0.279 |
| 4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
|
Resolution 3.86 Å R-free 0.317 |
| 4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 | Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
|
Resolution 3.86 Å R-free 0.317 |
| 4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
Resolution 3.50 Å R-free 0.309 |
| 4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 | Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
Resolution 3.50 Å R-free 0.309 |
| 4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
Resolution 3.50 Å R-free 0.328 |
| 4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 | Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
Resolution 3.50 Å R-free 0.328 |
| 4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 | Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
Resolution 4.00 Å R-free 0.329 |
| 4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 | Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
Resolution 4.00 Å R-free 0.329 |
| 4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain B7
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7；pH 7.0
|
Resolution 3.40 Å R-free 0.268 |
| 4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain D7
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7；pH 7.0
|
Resolution 3.40 Å R-free 0.268 |
| 4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain A4
1–48（48 aa）
|
Not recorded | BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.40 Å R-free 0.268 |
| 4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain C4
1–48（48 aa）
|
Not recorded | BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.40 Å R-free 0.268 |
| 4V9R Crystal structure of antibiotic DITYROMYCIN bound to 70S ribosome Deposited 2013-12-05 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1086 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 FME N-FORMYLMETHIONINE × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 3.00 Å R-free 0.259 |
| 4V9R Crystal structure of antibiotic DITYROMYCIN bound to 70S ribosome Deposited 2013-12-05 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 887 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 FME N-FORMYLMETHIONINE × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 3.00 Å R-free 0.259 |
| 4V9S Crystal structure of antibiotic GE82832 bound to 70S ribosome Deposited 2013-12-05 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1086 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 FME N-FORMYLMETHIONINE × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 3.10 Å R-free 0.260 |
| 4V9S Crystal structure of antibiotic GE82832 bound to 70S ribosome Deposited 2013-12-05 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 887 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 FME N-FORMYLMETHIONINE × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 3.10 Å R-free 0.260 |
| 4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 | Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
Resolution 3.80 Å R-free 0.331 |
| 4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 | Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
Resolution 3.80 Å R-free 0.331 |
| 4W2E Crystal structure of Elongation Factor 4 (EF4/LepA) bound to the Thermus thermophilus 70S ribosome Deposited 2014-06-04 | Assembly 1 Insufficient information Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 906 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.90 Å R-free 0.304 |
| 4W2F Crystal structure of the Thermus thermophilus 70S ribosome in complex with amicoumacin, mRNA and three deacylated tRNAs in the A, P and E sites Deposited 2014-09-12 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1338 UAM Amicoumacin A × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.40 Å R-free 0.277 |
| 4W2F Crystal structure of the Thermus thermophilus 70S ribosome in complex with amicoumacin, mRNA and three deacylated tRNAs in the A, P and E sites Deposited 2014-09-12 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 969 UAM Amicoumacin A × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.40 Å R-free 0.277 |
| 4W2G Crystal structure of the Thermus thermophilus 70S ribosome in complex with pactamycin (soaked), mRNA and three deacylated tRNAs in the A, P and E sites Deposited 2014-09-12 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1218 PCY Pactamycin × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.55 Å R-free 0.273 |
| 4W2G Crystal structure of the Thermus thermophilus 70S ribosome in complex with pactamycin (soaked), mRNA and three deacylated tRNAs in the A, P and E sites Deposited 2014-09-12 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 916 PCY Pactamycin × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.55 Å R-free 0.273 |
| 4W2H Crystal structure of the Thermus thermophilus 70S ribosome in complex with pactamycin (co-crystallized), mRNA and deacylated tRNA in the P site Deposited 2014-09-12 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1135 PCY Pactamycin × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.70 Å R-free 0.262 |
| 4W2H Crystal structure of the Thermus thermophilus 70S ribosome in complex with pactamycin (co-crystallized), mRNA and deacylated tRNA in the P site Deposited 2014-09-12 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 908 PCY Pactamycin × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.70 Å R-free 0.262 |
| 4W2I Crystal structure of the Thermus thermophilus 70S ribosome in complex with negamycin, mRNA and three deacylated tRNAs in the A, P and E sites Deposited 2014-09-12 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1147 NEG NEGAMYCIN × 9 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.70 Å R-free 0.257 |
| 4W2I Crystal structure of the Thermus thermophilus 70S ribosome in complex with negamycin, mRNA and three deacylated tRNAs in the A, P and E sites Deposited 2014-09-12 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 866 NEG NEGAMYCIN × 10 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.70 Å R-free 0.257 |
| 4W4G Postcleavage state of 70S bound to HigB toxin and AAA (lysine) codon Deposited 2014-08-14 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 581 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN
|
Resolution 3.30 Å R-free 0.238 |
| 4W4G Postcleavage state of 70S bound to HigB toxin and AAA (lysine) codon Deposited 2014-08-14 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 659 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN
|
Resolution 3.30 Å R-free 0.238 |
| 4WPO Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G in the pre-translocational state Deposited 2014-10-20 | Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain A7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1163 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.80 Å R-free 0.252 |
| 4WPO Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G in the pre-translocational state Deposited 2014-10-20 | Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain C7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 893 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.80 Å R-free 0.252 |
| 4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 618 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.10 Å R-free 0.235 |
| 4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 513 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.10 Å R-free 0.235 |
| 4WQF Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G and fusidic acid in the post-translocational state Deposited 2014-10-21 | Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain A7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1147 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.80 Å R-free 0.255 |
| 4WQF Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G and fusidic acid in the post-translocational state Deposited 2014-10-21 | Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain C7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 893 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.80 Å R-free 0.255 |
| 4WQR Complex of 70S ribosome with tRNA-Phe and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-22 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 726 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.15 Å R-free 0.231 |
| 4WQR Complex of 70S ribosome with tRNA-Phe and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-22 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 513 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.15 Å R-free 0.231 |
| 4WQU Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G trapped by the antibiotic dityromycin Deposited 2014-10-22 | Assembly 1 Insufficient information Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain A7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1147 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.80 Å R-free 0.264 |
| 4WQU Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G trapped by the antibiotic dityromycin Deposited 2014-10-22 | Assembly 2 Insufficient information Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain C7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 893 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.80 Å R-free 0.264 |
| 4WQY Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G in the post-translocational state (without fusitic acid) Deposited 2014-10-22 | Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain A7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1155 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.80 Å R-free 0.266 |
| 4WQY Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G in the post-translocational state (without fusitic acid) Deposited 2014-10-22 | Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain C7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 890 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.80 Å R-free 0.266 |
| 4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC（54） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 652 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.05 Å R-free 0.249 |
| 4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 | Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC（54） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 508 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.05 Å R-free 0.249 |
| 4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC（54） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 661 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.05 Å R-free 0.249 |
| 4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 | Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC（54） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 497 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.05 Å R-free 0.249 |
| 4WRO Complex of 70S ribosome with tRNA-Phe and mRNA with C-A mismatch in the second position in the A-site Deposited 2014-10-24 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 727 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.05 Å R-free 0.231 |
| 4WSD Complex of 70S ribosome with tRNA-Phe and mRNA with C-A mismatch in the second position in the A-site and with antibiotic paromomycin. Deposited 2014-10-27 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-MERIC（55） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 713 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 2.95 Å R-free 0.235 |
| 4WSD Complex of 70S ribosome with tRNA-Phe and mRNA with C-A mismatch in the second position in the A-site and with antibiotic paromomycin. Deposited 2014-10-27 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-MERIC（55） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 525 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 2.95 Å R-free 0.235 |
| 4WSM Complex of 70S ribosome with tRNA-Leu and mRNA with G-U mismatch in the first position in the A- and P-sites Deposited 2014-10-28 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 685 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.30 Å |
| 4WSM Complex of 70S ribosome with tRNA-Leu and mRNA with G-U mismatch in the first position in the A- and P-sites Deposited 2014-10-28 | Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 649 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.30 Å |
| 4WT1 Complex of 70S ribosome with tRNA-Phe and mRNA with A-A mismatch in the second position in the A-site Deposited 2014-10-29 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-MERIC（55） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 701 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.05 Å |
| 4WT1 Complex of 70S ribosome with tRNA-Phe and mRNA with A-A mismatch in the second position in the A-site Deposited 2014-10-29 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-MERIC（55） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 546 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.05 Å |
| 4WT8 Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex Deposited 2014-10-29 | Assembly 1 Insufficient information Heteromer；Protein × 51 PDB declaration: 61-meric（61） Review required |
Chain C8
1–48（48 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 3V6 Bactobolin A × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；293 K；0.2M KSCN, 0.1M Tris-HAc pH7, 3.5-4.5%(w/v) PEG20K, 3.5-
4.5%(w/v) PEG550MME
|
Resolution 3.40 Å R-free 0.241 |
| 4WT8 Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex Deposited 2014-10-29 | Assembly 2 Insufficient information Heteromer；Protein × 51 PDB declaration: 62-meric（62） Review required |
Chain D8
1–48（48 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 3V6 Bactobolin A × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；293 K；0.2M KSCN, 0.1M Tris-HAc pH7, 3.5-4.5%(w/v) PEG20K, 3.5-
4.5%(w/v) PEG550MME
|
Resolution 3.40 Å R-free 0.241 |
| 4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 708 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.20 Å R-free 0.238 |
| 4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 673 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.20 Å R-free 0.238 |
| 4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 699 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.10 Å R-free 0.193 |
| 4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 646 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.10 Å R-free 0.193 |
| 4WZO Complex of 70S ribosome with tRNA-fMet and mRNA Deposited 2014-11-20 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 56-MERIC（56） Review required |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 576 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.30 Å R-free 0.258 |
| 4WZO Complex of 70S ribosome with tRNA-fMet and mRNA Deposited 2014-11-20 | Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-MERIC（55） Review required |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 413 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.30 Å R-free 0.258 |
| 4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain AL34
1–48（48 aa）
|
Not recorded | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates.
Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes
mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C.
Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I
(100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
|
Resolution 3.80 Å R-free 0.284 |
| 4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain BL34
1–48（48 aa）
|
Not recorded | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates.
Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes
mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C.
Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I
(100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
|
Resolution 3.80 Å R-free 0.284 |
| 4Y4O Crystal structure of the Thermus thermophilus 70S ribosome with rRNA modifications and bound to protein Y (YfiA) at 2.3A resolution Deposited 2015-02-10 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1495 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.30 Å R-free 0.247 |
| 4Y4O Crystal structure of the Thermus thermophilus 70S ribosome with rRNA modifications and bound to protein Y (YfiA) at 2.3A resolution Deposited 2015-02-10 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1022 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.30 Å R-free 0.247 |
| 4Y4P Crystal structure of the Thermus thermophilus 70S ribosome with rRNA modifications and bound to mRNA and A-, P- and E-site tRNAs at 2.5A resolution Deposited 2015-02-10 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1572 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.281 |
| 4Y4P Crystal structure of the Thermus thermophilus 70S ribosome with rRNA modifications and bound to mRNA and A-, P- and E-site tRNAs at 2.5A resolution Deposited 2015-02-10 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1265 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.281 |
| 4YPB Precleavage 70S structure of the P. vulgaris HigB DeltaH92 toxin bound to the AAA codon Deposited 2015-03-12 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 624 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN
|
Resolution 3.40 Å R-free 0.234 |
| 4YPB Precleavage 70S structure of the P. vulgaris HigB DeltaH92 toxin bound to the AAA codon Deposited 2015-03-12 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 684 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN
|
Resolution 3.40 Å R-free 0.234 |
| 4YZV Precleavage 70S structure of the P. vulgaris HigB deltaH92 toxin bound to the ACA codon Deposited 2015-03-25 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 638 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN
|
Resolution 3.10 Å R-free 0.237 |
| 4YZV Precleavage 70S structure of the P. vulgaris HigB deltaH92 toxin bound to the ACA codon Deposited 2015-03-25 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 708 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN
|
Resolution 3.10 Å R-free 0.237 |
| 4Z3S Crystal structure of the Thermus thermophilus 70S ribosome in complex with antibiotic A201A, mRNA and three tRNAs in the A, P and E sites at 2.65A resolution Deposited 2015-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1328 4M2 3'-deoxy-3'-{[(2E)-3-(4-{[(4Z)-6-O-(6-deoxy-3,4-di-O-methyl-alpha-D-mannopyranosyl)-5-O-methyl-alpha-D-threo-hex-4-enofuranosyl]oxy}phenyl)-2-methylprop-2-enoyl]amino}-N,N-dimethyladenosine × 1 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.65 Å R-free 0.271 |
| 4Z3S Crystal structure of the Thermus thermophilus 70S ribosome in complex with antibiotic A201A, mRNA and three tRNAs in the A, P and E sites at 2.65A resolution Deposited 2015-03-31 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 964 4M2 3'-deoxy-3'-{[(2E)-3-(4-{[(4Z)-6-O-(6-deoxy-3,4-di-O-methyl-alpha-D-mannopyranosyl)-5-O-methyl-alpha-D-threo-hex-4-enofuranosyl]oxy}phenyl)-2-methylprop-2-enoyl]amino}-N,N-dimethyladenosine × 1 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.65 Å R-free 0.271 |
| 4Z8C Crystal structure of the Thermus thermophilus 70S ribosome bound to translation inhibitor oncocin Deposited 2015-04-08 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1448 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.9% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.90 Å R-free 0.248 |
| 4Z8C Crystal structure of the Thermus thermophilus 70S ribosome bound to translation inhibitor oncocin Deposited 2015-04-08 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 890 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.9% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.90 Å R-free 0.248 |
| 4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC（55） Consistent with all polymers |
Chain 17
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
|
Resolution 3.10 Å R-free 0.271 |
| 4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC（55） Consistent with all polymers |
Chain 27
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
|
Resolution 3.10 Å R-free 0.271 |
| 4ZSN 70S-wild-type HigB toxin complex bound to a AAA lysine codon Deposited 2015-05-13 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 610 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN
|
Resolution 3.60 Å R-free 0.234 |
| 4ZSN 70S-wild-type HigB toxin complex bound to a AAA lysine codon Deposited 2015-05-13 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 671 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN
|
Resolution 3.60 Å R-free 0.234 |
| 5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain Ad
1–49（49 aa）
|
Not recorded | NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions
Cryogen ETHANE；LIQUID ETHANE
|
Resolution 4.70 Å |
| 5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain Ad
1–49（49 aa）
|
Not recorded | NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions
Cryogen ETHANE；LIQUID ETHANE
|
Resolution 5.00 Å |
| 5CZP 70S termination complex containing E. coli RF2 Deposited 2015-07-31 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1027 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.9% PEG 20k, 9.8% PEG 200, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MAGNESIUM ACETATE
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.4% PEG 20k, 10.8% PEG 200, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MAGNESIUM ACETATE
|
Resolution 3.30 Å R-free 0.286 |
| 5CZP 70S termination complex containing E. coli RF2 Deposited 2015-07-31 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1493 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.9% PEG 20k, 9.8% PEG 200, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MAGNESIUM ACETATE
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.4% PEG 20k, 10.8% PEG 200, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MAGNESIUM ACETATE
|
Resolution 3.30 Å R-free 0.286 |
| 5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain AA
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
|
Resolution 3.63 Å R-free 0.287 |
| 5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain WB
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
|
Resolution 3.63 Å R-free 0.287 |
| 5DFE 70S termination complex containing E. coli RF2 Deposited 2015-08-26 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1488 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.5% PEG 20k, 11% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.8% PEG 20k, 11.6% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
|
Resolution 3.10 Å R-free 0.227 |
| 5DFE 70S termination complex containing E. coli RF2 Deposited 2015-08-26 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1025 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.5% PEG 20k, 11% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.8% PEG 20k, 11.6% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
|
Resolution 3.10 Å R-free 0.227 |
| 5DOX Crystal structure of the Thermus thermophilus 70S ribosome in complex with Hygromycin-A at 3.1A resolution Deposited 2015-09-11 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1019 HGR Hygromycin A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 3.10 Å R-free 0.284 |
| 5DOX Crystal structure of the Thermus thermophilus 70S ribosome in complex with Hygromycin-A at 3.1A resolution Deposited 2015-09-11 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 699 HGR Hygromycin A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 3.10 Å R-free 0.284 |
| 5DOY Crystal structure of the Thermus thermophilus 70S ribosome in complex with antibiotic Hygromycin A, mRNA and three tRNAs in the A, P and E sites at 2.6A resolution Deposited 2015-09-11 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1358 K POTASSIUM ION × 1 HGR Hygromycin A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.283 |
| 5DOY Crystal structure of the Thermus thermophilus 70S ribosome in complex with antibiotic Hygromycin A, mRNA and three tRNAs in the A, P and E sites at 2.6A resolution Deposited 2015-09-11 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 943 K POTASSIUM ION × 1 HGR Hygromycin A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.283 |
| 5E7K Structure of T. thermophilus 70S ribosome complex with mRNA and cognate tRNALys in the A-site Deposited 2015-10-12 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 628 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.20 Å R-free 0.258 |
| 5E7K Structure of T. thermophilus 70S ribosome complex with mRNA and cognate tRNALys in the A-site Deposited 2015-10-12 | Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 510 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.20 Å R-free 0.258 |
| 5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 2.95 Å R-free 0.242 |
| 5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 | Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric（55） Review required |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 2.95 Å R-free 0.242 |
| 5EL4 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position Deposited 2015-11-04 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 671 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.15 Å R-free 0.251 |
| 5EL4 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position Deposited 2015-11-04 | Assembly 2 Protein–RNA Heteromer；Protein × 46 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 536 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.15 Å R-free 0.251 |
| 5EL5 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the second position Deposited 2015-11-04 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-MERIC（55） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 660 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.15 Å R-free 0.254 |
| 5EL5 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the second position Deposited 2015-11-04 | Assembly 2 Protein–RNA Heteromer；Protein × 46 PDB declaration: 53-MERIC（53） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 522 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.15 Å R-free 0.254 |
| 5EL6 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position and antibiotic paromomycin Deposited 2015-11-04 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 593 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.10 Å R-free 0.249 |
| 5EL6 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position and antibiotic paromomycin Deposited 2015-11-04 | Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 480 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.10 Å R-free 0.249 |
| 5EL7 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the second position and antibiotic paromomycin Deposited 2015-11-04 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 687 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.15 Å R-free 0.247 |
| 5EL7 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the second position and antibiotic paromomycin Deposited 2015-11-04 | Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 574 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.15 Å R-free 0.247 |
| 5F8K Crystal structure of the Bac7(1-16) antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-09 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 17
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 1363 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
|
Resolution 2.80 Å R-free 0.292 |
| 5F8K Crystal structure of the Bac7(1-16) antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-09 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 27
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 1340 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
|
Resolution 2.80 Å R-free 0.292 |
| 5FDU Crystal structure of the Metalnikowin I antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-16 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 17
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 1292 AMP ADENOSINE MONOPHOSPHATE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
|
Resolution 2.90 Å R-free 0.234 |
| 5FDU Crystal structure of the Metalnikowin I antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-16 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 27
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 1127 AMP ADENOSINE MONOPHOSPHATE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
|
Resolution 2.90 Å R-free 0.234 |
| 5FDV Crystal structure of the Pyrrhocoricin antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-16 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 17
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 1292 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
|
Resolution 2.80 Å R-free 0.240 |
| 5FDV Crystal structure of the Pyrrhocoricin antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-16 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 27
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 1128 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 AMP ADENOSINE MONOPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
|
Resolution 2.80 Å R-free 0.240 |
| 5HAU Crystal structure of antimicrobial peptide Bac7(1-19) bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-30 | Assembly 1 Insufficient information Heteromer；Protein × 53 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain 15
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1081 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
|
Resolution 3.00 Å R-free 0.265 |
| 5HAU Crystal structure of antimicrobial peptide Bac7(1-19) bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-30 | Assembly 2 Insufficient information Heteromer；Protein × 53 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain 25
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 848 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
|
Resolution 3.00 Å R-free 0.265 |
| 5HCP Crystal structure of antimicrobial peptide Metalnikowin bound to the Thermus thermophilus 70S ribosome Deposited 2016-01-04 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1593 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
|
Resolution 2.89 Å R-free 0.268 |
| 5HCP Crystal structure of antimicrobial peptide Metalnikowin bound to the Thermus thermophilus 70S ribosome Deposited 2016-01-04 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 887 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
|
Resolution 2.89 Å R-free 0.268 |
| 5HCQ Crystal structure of antimicrobial peptide Oncocin d15-19 bound to the Thermus thermophilus 70S ribosome Deposited 2016-01-04 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1540 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.251 |
| 5HCQ Crystal structure of antimicrobial peptide Oncocin d15-19 bound to the Thermus thermophilus 70S ribosome Deposited 2016-01-04 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 890 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.251 |
| 5HCR Crystal structure of antimicrobial peptide Oncocin 10wt bound to the Thermus thermophilus 70S ribosome Deposited 2016-01-04 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1585 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.268 |
| 5HCR Crystal structure of antimicrobial peptide Oncocin 10wt bound to the Thermus thermophilus 70S ribosome Deposited 2016-01-04 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 837 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.268 |
| 5HD1 Crystal structure of antimicrobial peptide Pyrrhocoricin bound to the Thermus thermophilus 70S ribosome Deposited 2016-01-04 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1701 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.272 |
| 5HD1 Crystal structure of antimicrobial peptide Pyrrhocoricin bound to the Thermus thermophilus 70S ribosome Deposited 2016-01-04 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 900 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.272 |
| 5IB7 Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet, near-cognate tRNALys with U-G mismatch in the A-site and antibiotic paromomycin Deposited 2016-02-22 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 695 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 2.99 Å R-free 0.241 |
| 5IB7 Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet, near-cognate tRNALys with U-G mismatch in the A-site and antibiotic paromomycin Deposited 2016-02-22 | Assembly 2 Protein–RNA Heteromer；Protein × 46 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 563 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 SPE THERMINE × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 2.99 Å R-free 0.241 |
| 5IB8 Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and near-cognate tRNALys with U-G mismatch in the A-site Deposited 2016-02-22 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 723 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.13 Å R-free 0.244 |
| 5IB8 Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and near-cognate tRNALys with U-G mismatch in the A-site Deposited 2016-02-22 | Assembly 2 Protein–RNA Heteromer；Protein × 46 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 614 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 SPE THERMINE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.13 Å R-free 0.244 |
| 5IBB Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAVal in the A-site Deposited 2016-02-22 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 736 SPE THERMINE × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 2.96 Å R-free 0.243 |
| 5IBB Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAVal in the A-site Deposited 2016-02-22 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 608 SPE THERMINE × 4 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 2.96 Å R-free 0.243 |
| 5IMQ Structure of ribosome bound to cofactor at 3.8 angstrom resolution Deposited 2016-03-06 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain y
1–49（49 aa）
|
Not recorded | GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Buffer G
5 mM HEPES pH 7.5, 10 mM MgAc, 50 mM KCl, 10 mM NH4Cl, and 6 mM 2-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 5IMR Structure of ribosome bound to cofactor at 5.7 angstrom resolution Deposited 2016-03-06 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain y
1–49（49 aa）
|
Not recorded | GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Buffer G
5 mM HEPES pH 7.5, 10 mM MgAc, 50 mM KCl, 10 mM NH4Cl, and 6 mM 2-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.70 Å |
| 5J30 Thermus thermophilus 70S termination complex containing E. coli RF1 Deposited 2016-03-30 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1431 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.1% PEG 20k, 10.2% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
|
Resolution 3.20 Å R-free 0.244 |
| 5J30 Thermus thermophilus 70S termination complex containing E. coli RF1 Deposited 2016-03-30 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1000 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.1% PEG 20k, 10.2% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
|
Resolution 3.20 Å R-free 0.244 |
| 5J3C Thermus thermophilus 70S termination complex containing E. coli RF1 Deposited 2016-03-30 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1425 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.2% PEG 20k, 10.4% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
|
Resolution 3.04 Å R-free 0.279 |
| 5J3C Thermus thermophilus 70S termination complex containing E. coli RF1 Deposited 2016-03-30 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1011 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.2% PEG 20k, 10.4% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
|
Resolution 3.04 Å R-free 0.279 |
| 5J4B Crystal structure of the Thermus thermophilus 70S ribosome in complex with cisplatin (co-crystallized) and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution Deposited 2016-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1706 K POTASSIUM ION × 1 CPT Cisplatin × 9 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.264 |
| 5J4B Crystal structure of the Thermus thermophilus 70S ribosome in complex with cisplatin (co-crystallized) and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution Deposited 2016-03-31 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1311 K POTASSIUM ION × 1 CPT Cisplatin × 7 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.264 |
| 5J4C Crystal structure of the Thermus thermophilus 70S ribosome in complex with cisplatin (soaked) and bound to mRNA and A-, P- and E-site tRNAs at 2.8A resolution Deposited 2016-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1796 CPT Cisplatin × 4 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.268 |
| 5J4C Crystal structure of the Thermus thermophilus 70S ribosome in complex with cisplatin (soaked) and bound to mRNA and A-, P- and E-site tRNAs at 2.8A resolution Deposited 2016-03-31 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1275 CPT Cisplatin × 4 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.268 |
| 5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain EA
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
|
Resolution 3.10 Å R-free 0.256 |
| 5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain JC
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
|
Resolution 3.10 Å R-free 0.256 |
| 5J8B Crystal structure of Elongation Factor 4 (EF-4/LepA) in complex with GDPCP bound to the Thermus thermophilus 70S ribosome Deposited 2016-04-07 | Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain 7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 919 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.60 Å R-free 0.261 |
| 5NDJ Crystal structure of aminoglycoside TC007 in complex with 70S ribosome from Thermus thermophilus, three tRNAs and mRNA (soaking) Deposited 2017-03-08 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | 8UZ TC007 × 6 MG MAGNESIUM ION × 931 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN
|
Resolution 3.15 Å R-free 0.247 |
| 5NDJ Crystal structure of aminoglycoside TC007 in complex with 70S ribosome from Thermus thermophilus, three tRNAs and mRNA (soaking) Deposited 2017-03-08 | Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | 8UZ TC007 × 7 MG MAGNESIUM ION × 839 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN
|
Resolution 3.15 Å R-free 0.247 |
| 5NDK Crystal structure of aminoglycoside TC007 co-crystallized with 70S ribosome from Thermus thermophilus, three tRNAs and mRNA Deposited 2017-03-08 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | 8UZ TC007 × 2 MG MAGNESIUM ION × 846 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN
|
Resolution 2.95 Å R-free 0.253 |
| 5NDK Crystal structure of aminoglycoside TC007 co-crystallized with 70S ribosome from Thermus thermophilus, three tRNAs and mRNA Deposited 2017-03-08 | Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | 8UZ TC007 × 2 MG MAGNESIUM ION × 800 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN
|
Resolution 2.95 Å R-free 0.253 |
| 5OT7 Elongation factor G-ribosome complex captures in the absence of inhibitors. Deposited 2017-08-21 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain c
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 462 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 5UQ7 70S ribosome complex with dnaX mRNA stemloop and E-site tRNA ("in" conformation) Deposited 2017-02-07 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 7
1–48（48 aa）
|
Not recorded | ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 5UQ8 70S ribosome complex with dnaX mRNA stem-loop and E-site tRNA ("out" conformation) Deposited 2017-02-07 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 7
1–48（48 aa）
|
Not recorded | ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
|
Resolution 3.25 Å R-free 0.270 |
| 5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
|
Resolution 3.25 Å R-free 0.270 |
| 5VP2 Crystal structure of the Thermus thermophilus 70S ribosome in complex with madumycin II and bound to mRNA and A-, P- and E-site tRNAs at 2.8A resolution Deposited 2017-05-04 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1587 K POTASSIUM ION × 1 M2D Madumycin II × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.278 |
| 5VP2 Crystal structure of the Thermus thermophilus 70S ribosome in complex with madumycin II and bound to mRNA and A-, P- and E-site tRNAs at 2.8A resolution Deposited 2017-05-04 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1216 K POTASSIUM ION × 1 M2D Madumycin II × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.278 |
| 5VPO The 70S P-site ASL SufA6 complex Deposited 2017-05-05 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 325 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5% PEG 20k, 5% PEG 550 MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM CHLORIDE
|
Resolution 3.34 Å R-free 0.267 |
| 5VPO The 70S P-site ASL SufA6 complex Deposited 2017-05-05 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 352 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5% PEG 20k, 5% PEG 550 MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM CHLORIDE
|
Resolution 3.34 Å R-free 0.267 |
| 5VPP The 70S P-site tRNA SufA6 complex Deposited 2017-05-05 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 768 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5% PEG 20k, 5% PEG 550 MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM CHLORIDE
|
Resolution 3.90 Å |
| 5VPP The 70S P-site tRNA SufA6 complex Deposited 2017-05-05 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 547 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5% PEG 20k, 5% PEG 550 MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM CHLORIDE
|
Resolution 3.90 Å |
| 5W4K Crystal structure of the Thermus thermophilus 70S ribosome in complex with Klebsazolicin and bound to mRNA and A-, P- and E-site tRNAs at 2.7A resolution Deposited 2017-06-12 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1724 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.252 |
| 5W4K Crystal structure of the Thermus thermophilus 70S ribosome in complex with Klebsazolicin and bound to mRNA and A-, P- and E-site tRNAs at 2.7A resolution Deposited 2017-06-12 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1201 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.252 |
| 5WIS Crystal structure of the Thermus thermophilus 70S ribosome in complex with methymycin and bound to mRNA and A-, P- and E-site tRNAs at 2.7A resolution Deposited 2017-07-20 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1587 MT9 (3R,4S,5S,7R,9E,11S,12R)-12-ethyl-11-hydroxy-3,5,7,11-tetramethyl-2,8-dioxooxacyclododec-9-en-4-yl 3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranoside × 1 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.266 |
| 5WIS Crystal structure of the Thermus thermophilus 70S ribosome in complex with methymycin and bound to mRNA and A-, P- and E-site tRNAs at 2.7A resolution Deposited 2017-07-20 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1262 MT9 (3R,4S,5S,7R,9E,11S,12R)-12-ethyl-11-hydroxy-3,5,7,11-tetramethyl-2,8-dioxooxacyclododec-9-en-4-yl 3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranoside × 1 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.266 |
| 5WIT Crystal structure of the Thermus thermophilus 70S ribosome in complex with pikromycin and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution Deposited 2017-07-20 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1411 K POTASSIUM ION × 1 AQJ (3R,5R,6S,7S,9R,11E,13S,14R)-14-ethyl-13-hydroxy-3,5,7,9,13-pentamethyl-2,4,10-trioxo-1-oxacyclotetradec-11-en-6-yl 3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranoside × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.283 |
| 5WIT Crystal structure of the Thermus thermophilus 70S ribosome in complex with pikromycin and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution Deposited 2017-07-20 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 982 K POTASSIUM ION × 1 AQJ (3R,5R,6S,7S,9R,11E,13S,14R)-14-ethyl-13-hydroxy-3,5,7,9,13-pentamethyl-2,4,10-trioxo-1-oxacyclotetradec-11-en-6-yl 3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranoside × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.283 |
| 5ZLU Ribosome Structure bound to ABC-F protein. Deposited 2018-03-29 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain z
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain EA
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
|
Resolution 3.40 Å |
| 6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain IC
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
|
Resolution 3.40 Å |
| 6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain EA
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
|
Resolution 3.40 Å |
| 6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain JC
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
|
Resolution 3.40 Å |
| 6BUW Thermus thermophilus 70S complex containing 16S G299A ram mutation and empty A site. Deposited 2017-12-11 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 719 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.8% PEG 20k, 3.8% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
|
Resolution 3.50 Å R-free 0.250 |
| 6BUW Thermus thermophilus 70S complex containing 16S G299A ram mutation and empty A site. Deposited 2017-12-11 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 731 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.8% PEG 20k, 3.8% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
|
Resolution 3.50 Å R-free 0.250 |
| 6BZ6 Thermus thermophilus 70S complex containing 16S G347U ram mutation and empty A site Deposited 2017-12-22 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 641 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.8% PEG 20k, 4.8% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.9% PEG 20k, 4.9% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
|
Resolution 3.18 Å R-free 0.251 |
| 6BZ6 Thermus thermophilus 70S complex containing 16S G347U ram mutation and empty A site Deposited 2017-12-22 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 703 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.8% PEG 20k, 4.8% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.9% PEG 20k, 4.9% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
|
Resolution 3.18 Å R-free 0.251 |
| 6BZ7 Thermus thermophilus 70S containing 16S G299A point mutation and near-cognate ASL Leucine in A site. Deposited 2017-12-22 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 618 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.8% PEG 20k, 3.8% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.1% PEG 20k, 4.1% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
|
Resolution 3.68 Å R-free 0.274 |
| 6BZ7 Thermus thermophilus 70S containing 16S G299A point mutation and near-cognate ASL Leucine in A site. Deposited 2017-12-22 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 669 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.8% PEG 20k, 3.8% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.1% PEG 20k, 4.1% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
|
Resolution 3.68 Å R-free 0.274 |
| 6BZ8 Thermus thermophilus 70S containing 16S G347U point mutation and near-cognate ASL Leucine in A site Deposited 2017-12-22 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 624 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.7% PEG 20k, 4.7% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
|
Resolution 3.74 Å R-free 0.225 |
| 6BZ8 Thermus thermophilus 70S containing 16S G347U point mutation and near-cognate ASL Leucine in A site Deposited 2017-12-22 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 668 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.7% PEG 20k, 4.7% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
|
Resolution 3.74 Å R-free 0.225 |
| 6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 218 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
|
Resolution 3.20 Å R-free 0.247 |
| 6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 | Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
|
Resolution 3.20 Å R-free 0.247 |
| 6CAE Crystal structure of the Thermus thermophilus 70S ribosome in complex with NOSO-95179 antibiotic and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution Deposited 2018-01-30 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1651 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.249 |
| 6CAE Crystal structure of the Thermus thermophilus 70S ribosome in complex with NOSO-95179 antibiotic and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution Deposited 2018-01-30 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1175 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.249 |
| 6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.269 |
| 6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.269 |
| 6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.253 |
| 6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.253 |
| 6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.252 |
| 6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.252 |
| 6CZR The structure of amicetin bound to the 70S ribosome Deposited 2018-04-09 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain 17
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 1163 ARG ARGININE × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 FSD amicetin × 1 ZN ZINC ION × 4 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；292 K；0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
|
Resolution 3.14 Å R-free 0.292 |
| 6CZR The structure of amicetin bound to the 70S ribosome Deposited 2018-04-09 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain 27
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 770 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 FSD amicetin × 1 ZN ZINC ION × 5 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；292 K；0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
|
Resolution 3.14 Å R-free 0.292 |
| 6FKR Crystal structure of the dolphin proline-rich antimicrobial peptide Tur1A bound to the Thermus thermophilus 70S ribosome Deposited 2018-01-24 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 53-meric（53） Review required |
Chain 17
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 1347 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl pH 7.6, PEG 20,000, 2-methyl-2,4-pentanediol, L-arginine
|
Resolution 3.20 Å R-free 0.252 |
| 6FKR Crystal structure of the dolphin proline-rich antimicrobial peptide Tur1A bound to the Thermus thermophilus 70S ribosome Deposited 2018-01-24 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 27
1–48（48 aa）
|
Not recorded | MG MAGNESIUM ION × 1347 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl pH 7.6, PEG 20,000, 2-methyl-2,4-pentanediol, L-arginine
|
Resolution 3.20 Å R-free 0.252 |
| 6GSJ Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAThr in the A-site Deposited 2018-06-14 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 874 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；Tris-OAc, KSCN, PEG-20000, PEG-550mme
|
Resolution 2.96 Å R-free 0.252 |
| 6GSJ Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAThr in the A-site Deposited 2018-06-14 | Assembly 2 Protein–RNA Heteromer；Protein × 46 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 620 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；Tris-OAc, KSCN, PEG-20000, PEG-550mme
|
Resolution 2.96 Å R-free 0.252 |
| 6GSK Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and near-cognate tRNAThr in the A-site Deposited 2018-06-14 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 638 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；Tris-OAc, KSCN, PEG-20000, PEG-550mme
|
Resolution 3.36 Å R-free 0.264 |
| 6GSK Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and near-cognate tRNAThr in the A-site Deposited 2018-06-14 | Assembly 2 Protein–RNA Heteromer；Protein × 46 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 444 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；Tris-OAc, KSCN, PEG-20000, PEG-550mme
|
Resolution 3.36 Å R-free 0.264 |
| 6GSL Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAArg in the A-site Deposited 2018-06-14 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 954 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；Tris-OAc, KSCN, PEG-20000, PEG-550mme
|
Resolution 3.16 Å |
| 6GSL Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAArg in the A-site Deposited 2018-06-14 | Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 810 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；Tris-OAc, KSCN, PEG-20000, PEG-550mme
|
Resolution 3.16 Å |
| 6GZQ T. thermophilus hibernating 70S ribosome Deposited 2018-07-04 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain c1
1–49（49 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.28 Å |
| 6GZX T. thermophilus hibernating 100S ribosome (ice) Deposited 2018-07-05 | Assembly 1 Protein–RNA Heteromer；Protein × 102 PDB declaration: 108-meric（108） Consistent with all polymers |
Chain c1
1–49（49 aa）
Chain c2
1–49（49 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.57 Å |
| 6GZZ T. thermophilus hibernating 100S ribosome (amc) Deposited 2018-07-05 | Assembly 1 Protein–RNA Heteromer；Protein × 102 PDB declaration: 108-meric（108） Consistent with all polymers |
Chain c1
1–49（49 aa）
Chain c2
1–49（49 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.13 Å |
| 6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain AL34
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
Resolution 3.20 Å R-free 0.321 |
| 6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain BL34
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
Resolution 3.20 Å R-free 0.321 |
| 6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 632 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 3.70 Å R-free 0.278 |
| 6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 524 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 3.70 Å R-free 0.278 |
| 6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 710 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 3.70 Å R-free 0.279 |
| 6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 629 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 3.70 Å R-free 0.279 |
| 6ND5 Crystal structure of the Thermus thermophilus 70S ribosome in complex with chloramphenicol and bound to mRNA and A-, P-, and E-site tRNAs at 2.60A resolution Deposited 2018-12-13 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1576 CLM CHLORAMPHENICOL × 1 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.276 |
| 6ND5 Crystal structure of the Thermus thermophilus 70S ribosome in complex with chloramphenicol and bound to mRNA and A-, P-, and E-site tRNAs at 2.60A resolution Deposited 2018-12-13 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1246 CLM CHLORAMPHENICOL × 1 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.276 |
| 6ND6 Crystal structure of the Thermus thermophilus 70S ribosome in complex with erythromycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.85A resolution Deposited 2018-12-13 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1523 ERY ERYTHROMYCIN A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.85 Å R-free 0.305 |
| 6ND6 Crystal structure of the Thermus thermophilus 70S ribosome in complex with erythromycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.85A resolution Deposited 2018-12-13 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1222 ERY ERYTHROMYCIN A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.85 Å R-free 0.305 |
| 6NDK Structure of ASLSufA6 A37.5 bound to the 70S A site Deposited 2018-12-13 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 596 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4.5-5.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
|
Resolution 3.64 Å R-free 0.250 |
| 6NDK Structure of ASLSufA6 A37.5 bound to the 70S A site Deposited 2018-12-13 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 725 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4.5-5.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
|
Resolution 3.64 Å R-free 0.250 |
| 6NSH Modified ASL proline bound to Thermus thermophilus 70S (near-cognate) Deposited 2019-01-24 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 575 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME
|
Resolution 3.40 Å R-free 0.237 |
| 6NSH Modified ASL proline bound to Thermus thermophilus 70S (near-cognate) Deposited 2019-01-24 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 624 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME
|
Resolution 3.40 Å R-free 0.237 |
| 6NTA Modified ASL proline bound to Thermus thermophilus 70S (cognate) Deposited 2019-01-28 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 592 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME
|
Resolution 3.10 Å R-free 0.274 |
| 6NTA Modified ASL proline bound to Thermus thermophilus 70S (cognate) Deposited 2019-01-28 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 679 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME
|
Resolution 3.10 Å R-free 0.274 |
| 6NUO Modified tRNA(Pro) bound to Thermus thermophilus 70S (cognate) Deposited 2019-02-01 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 531 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME
|
Resolution 3.20 Å R-free 0.256 |
| 6NUO Modified tRNA(Pro) bound to Thermus thermophilus 70S (cognate) Deposited 2019-02-01 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 621 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME
|
Resolution 3.20 Å R-free 0.256 |
| 6NWY Modified tRNA(Pro) bound to Thermus thermophilus 70S (near-cognate) Deposited 2019-02-07 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 547 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME
|
Resolution 3.50 Å R-free 0.258 |
| 6NWY Modified tRNA(Pro) bound to Thermus thermophilus 70S (near-cognate) Deposited 2019-02-07 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 551 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME
|
Resolution 3.50 Å R-free 0.258 |
| 6O3M Unmodified tRNA(Pro) bound to Thermus thermophilus 70S (cognate) Deposited 2019-02-26 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 466 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；12% MPD 0.1M, Arginine-HCl, 0.1M Tris-HCl pH7.6, 2.9% PEG 20K, 0.0006M BME
|
Resolution 3.97 Å R-free 0.255 |
| 6O3M Unmodified tRNA(Pro) bound to Thermus thermophilus 70S (cognate) Deposited 2019-02-26 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 542 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；12% MPD 0.1M, Arginine-HCl, 0.1M Tris-HCl pH7.6, 2.9% PEG 20K, 0.0006M BME
|
Resolution 3.97 Å R-free 0.255 |
| 6O97 Crystal structure of the Thermus thermophilus 70S ribosome in complex with propylamycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.75A resolution Deposited 2019-03-13 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1565 LUJ (1R,2R,3S,4R,6S)-4,6-diamino-2-{[3-O-(2,6-diamino-2,6-dideoxy-beta-L-idopyranosyl)-beta-D-ribofuranosyl]oxy}-3-hydroxyc yclohexyl 2-amino-2,4-dideoxy-4-propyl-alpha-D-glucopyranoside × 3 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.75 Å R-free 0.284 |
| 6O97 Crystal structure of the Thermus thermophilus 70S ribosome in complex with propylamycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.75A resolution Deposited 2019-03-13 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1191 LUJ (1R,2R,3S,4R,6S)-4,6-diamino-2-{[3-O-(2,6-diamino-2,6-dideoxy-beta-L-idopyranosyl)-beta-D-ribofuranosyl]oxy}-3-hydroxyc yclohexyl 2-amino-2,4-dideoxy-4-propyl-alpha-D-glucopyranoside × 3 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.75 Å R-free 0.284 |
| 6OF1 Crystal structure of the Thermus thermophilus 70S ribosome in complex with dirithromycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.80A resolution Deposited 2019-03-28 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1589 DI0 Dirithromycin × 1 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.263 |
| 6OF1 Crystal structure of the Thermus thermophilus 70S ribosome in complex with dirithromycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.80A resolution Deposited 2019-03-28 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1194 DI0 Dirithromycin × 1 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.263 |
| 6OF6 Crystal structure of tRNA^ Ala(GGC) bound to cognate 70S A-site Deposited 2019-03-28 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 546 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 AMP ADENOSINE MONOPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
|
Resolution 3.20 Å R-free 0.232 |
| 6OF6 Crystal structure of tRNA^ Ala(GGC) bound to cognate 70S A-site Deposited 2019-03-28 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 669 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
|
Resolution 3.20 Å R-free 0.232 |
| 6OJ2 Crystal structure of tRNA^ Ala(GGC) bound to the near-cognate 70S A-site Deposited 2019-04-10 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 433 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
|
Resolution 3.20 Å R-free 0.270 |
| 6OJ2 Crystal structure of tRNA^ Ala(GGC) bound to the near-cognate 70S A-site Deposited 2019-04-10 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 517 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
|
Resolution 3.20 Å R-free 0.270 |
| 6OPE Crystal structure of tRNA^ Ala(GGC) U32-A38 bound to near-cognate 70S A site Deposited 2019-04-24 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 378 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1M L-arginine HCl, 0.1M Tris-HCl pH 7.5, 3% PEG 20K, 10-16.5% MPD (2-methyl-2,4-pentanediol), 1mM BME
|
Resolution 3.10 Å R-free 0.266 |
| 6OPE Crystal structure of tRNA^ Ala(GGC) U32-A38 bound to near-cognate 70S A site Deposited 2019-04-24 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 469 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1M L-arginine HCl, 0.1M Tris-HCl pH 7.5, 3% PEG 20K, 10-16.5% MPD (2-methyl-2,4-pentanediol), 1mM BME
|
Resolution 3.10 Å R-free 0.266 |
| 6ORD Crystal structure of tRNA^ Ala(GGC) U32-A38 bound to cognate 70S A site Deposited 2019-04-30 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1036 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1M L-arginine HCl, 0.1M Tris-HCl pH 7.5, 3% PEG 20K, 10-16.5% MPD (2-methyl-2,4-pentanediol), 1mM BME
|
Resolution 3.10 Å R-free 0.268 |
| 6ORD Crystal structure of tRNA^ Ala(GGC) U32-A38 bound to cognate 70S A site Deposited 2019-04-30 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 975 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1M L-arginine HCl, 0.1M Tris-HCl pH 7.5, 3% PEG 20K, 10-16.5% MPD (2-methyl-2,4-pentanediol), 1mM BME
|
Resolution 3.10 Å R-free 0.268 |
| 6OSI Unmodified tRNA(Pro) bound to Thermus thermophilus 70S (near cognate) Deposited 2019-05-01 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 522 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
|
Resolution 4.14 Å R-free 0.294 |
| 6OSI Unmodified tRNA(Pro) bound to Thermus thermophilus 70S (near cognate) Deposited 2019-05-01 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 504 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
|
Resolution 4.14 Å R-free 0.294 |
| 6OTR Dimeric E.coli YoeB bound to Thermus thermophilus 70S post-cleavage (AAU) Deposited 2019-05-03 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 410 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 3 A3P ADENOSINE-3'-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；0.1M Tris-HAc, 0.2M KSCN, PEG 5.1%, 5.1% PEG550MME
|
Resolution 3.12 Å R-free 0.257 |
| 6OTR Dimeric E.coli YoeB bound to Thermus thermophilus 70S post-cleavage (AAU) Deposited 2019-05-03 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 464 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 A3P ADENOSINE-3'-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；0.1M Tris-HAc, 0.2M KSCN, PEG 5.1%, 5.1% PEG550MME
|
Resolution 3.12 Å R-free 0.257 |
| 6OXA Dimeric E.coli YoeB bound to Thermus thermophilus 70S pre-cleavage (AAU) Deposited 2019-05-13 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 420 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；0.1 M Tris-HAc, 0.2M KSCN, PEG 5.1%, 5.1% PEG550MME
|
Resolution 3.25 Å R-free 0.256 |
| 6OXA Dimeric E.coli YoeB bound to Thermus thermophilus 70S pre-cleavage (AAU) Deposited 2019-05-13 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 476 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；0.1 M Tris-HAc, 0.2M KSCN, PEG 5.1%, 5.1% PEG550MME
|
Resolution 3.25 Å R-free 0.256 |
| 6OXI Dimeric E.coli YoeB bound to Thermus thermophilus 70S post-cleavage (UAA) Deposited 2019-05-13 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 419 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；0.1 M Tris-HAc, 0.2 M KSCN PEG 5.2%, 5.2% PEG550MME
|
Resolution 3.50 Å R-free 0.246 |
| 6OXI Dimeric E.coli YoeB bound to Thermus thermophilus 70S post-cleavage (UAA) Deposited 2019-05-13 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 471 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；0.1 M Tris-HAc, 0.2 M KSCN PEG 5.2%, 5.2% PEG550MME
|
Resolution 3.50 Å R-free 0.246 |
| 6Q95 Structure of tmRNA SmpB bound in A site of T. thermophilus 70S ribosome Deposited 2018-12-17 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain d
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 637 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6QNQ 70S ribosome initiation complex (IC) with experimentally assigned potassium ions Deposited 2019-02-11 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | K POTASSIUM ION × 169 MG MAGNESIUM ION × 543 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；Tris-OAc, KSCN, PEG-20K, PEG-550mme
|
Resolution 3.50 Å R-free 0.251 |
| 6QNQ 70S ribosome initiation complex (IC) with experimentally assigned potassium ions Deposited 2019-02-11 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | K POTASSIUM ION × 127 MG MAGNESIUM ION × 426 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；Tris-OAc, KSCN, PEG-20K, PEG-550mme
|
Resolution 3.50 Å R-free 0.251 |
| 6QNR 70S ribosome elongation complex (EC) with experimentally assigned potassium ions Deposited 2019-02-11 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain P8
1–49（49 aa）
|
Not recorded | K POTASSIUM ION × 211 MG MAGNESIUM ION × 585 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Tris-OAc, KSCN, PEG-20k, PEG-550mme
|
Resolution 3.10 Å R-free 0.253 |
| 6QNR 70S ribosome elongation complex (EC) with experimentally assigned potassium ions Deposited 2019-02-11 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain L5
1–49（49 aa）
|
Not recorded | K POTASSIUM ION × 155 MG MAGNESIUM ION × 409 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Tris-OAc, KSCN, PEG-20k, PEG-550mme
|
Resolution 3.10 Å R-free 0.253 |
| 6UCQ Crystal structure of the Thermus thermophilus 70S ribosome recycling complex Deposited 2019-09-17 | Assembly 1 Insufficient information Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1523 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.3；292 K；0.15 M L-arginine-HCl, 0.1M Tris-HCl pH 7.3, 2.4-2.7% PEG-20K, 7-9% MPD, 0.5 mM BME
|
Resolution 3.50 Å R-free 0.294 |
| 6UCQ Crystal structure of the Thermus thermophilus 70S ribosome recycling complex Deposited 2019-09-17 | Assembly 2 Insufficient information Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1350 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.3；292 K；0.15 M L-arginine-HCl, 0.1M Tris-HCl pH 7.3, 2.4-2.7% PEG-20K, 7-9% MPD, 0.5 mM BME
|
Resolution 3.50 Å R-free 0.294 |
| 6UO1 Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA (containing pseudouridine at the first position of the codon) and deacylated A-, P-, and E-site tRNAs at 2.95A resolution Deposited 2019-10-14 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 918 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.95 Å R-free 0.292 |
| 6UO1 Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA (containing pseudouridine at the first position of the codon) and deacylated A-, P-, and E-site tRNAs at 2.95A resolution Deposited 2019-10-14 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 534 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.95 Å R-free 0.292 |
| 6XHV Crystal structure of the A2058-dimethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A- and P-site tRNAs, and deacylated E-site tRNA at 2.40A resolution Deposited 2020-06-19 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1568 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.40 Å R-free 0.286 |
| 6XHV Crystal structure of the A2058-dimethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A- and P-site tRNAs, and deacylated E-site tRNA at 2.40A resolution Deposited 2020-06-19 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1248 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.40 Å R-free 0.286 |
| 6XHW Crystal structure of the A2058-unmethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A- and P-site tRNAs, and deacylated E-site tRNA at 2.50A resolution Deposited 2020-06-19 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1552 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.281 |
| 6XHW Crystal structure of the A2058-unmethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A- and P-site tRNAs, and deacylated E-site tRNA at 2.50A resolution Deposited 2020-06-19 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1244 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.281 |
| 6XHX Crystal structure of the A2058-unmethylated Thermus thermophilus 70S ribosome in complex with erythromycin and protein Y (YfiA) at 2.55A resolution Deposited 2020-06-19 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1479 ERY ERYTHROMYCIN A × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.272 |
| 6XHX Crystal structure of the A2058-unmethylated Thermus thermophilus 70S ribosome in complex with erythromycin and protein Y (YfiA) at 2.55A resolution Deposited 2020-06-19 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 995 ERY ERYTHROMYCIN A × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.272 |
| 6XHY Crystal structure of the Thermus thermophilus 70S ribosome in complex with telithromycin, mRNA, aminoacylated A- and P-site tRNAs, and deacylated E-site tRNA at 2.60A resolution Deposited 2020-06-19 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1510 K POTASSIUM ION × 1 TEL TELITHROMYCIN × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.266 |
| 6XHY Crystal structure of the Thermus thermophilus 70S ribosome in complex with telithromycin, mRNA, aminoacylated A- and P-site tRNAs, and deacylated E-site tRNA at 2.60A resolution Deposited 2020-06-19 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1165 K POTASSIUM ION × 1 TEL TELITHROMYCIN × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.266 |
| 6XQD Crystal structure of the Thermus thermophilus 70S ribosome in complex with sarecycline, UUC-mRNA, and deacylated P-site tRNA at 2.80A resolution Deposited 2020-07-09 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1535 ZN ZINC ION × 6 V7A Sarecycline × 1 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.276 |
| 6XQD Crystal structure of the Thermus thermophilus 70S ribosome in complex with sarecycline, UUC-mRNA, and deacylated P-site tRNA at 2.80A resolution Deposited 2020-07-09 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1164 ZN ZINC ION × 6 V7A Sarecycline × 1 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.276 |
| 6XQE Crystal structure of the Thermus thermophilus 70S ribosome in complex with sarecycline, UAA-mRNA, and deacylated P-site tRNA at 3.00A resolution Deposited 2020-07-09 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1465 K POTASSIUM ION × 1 ZN ZINC ION × 6 V7A Sarecycline × 1 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 3.00 Å R-free 0.311 |
| 6XQE Crystal structure of the Thermus thermophilus 70S ribosome in complex with sarecycline, UAA-mRNA, and deacylated P-site tRNA at 3.00A resolution Deposited 2020-07-09 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1173 K POTASSIUM ION × 1 ZN ZINC ION × 6 V7A Sarecycline × 1 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 3.00 Å R-free 0.311 |
| 7AZO 70S thermus thermophilus ribosome with bound antibiotic lead SEQ-977 Deposited 2020-11-17 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain L34A
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 448 OHX osmium (III) hexammine × 318 K POTASSIUM ION × 159 SJE (2R,3S,4R,5R,7S,9S,10S,11R,12S,13R)-12-(((2R,4R,5S,6S)-4,5-dihydroxy-4,6-dimethyltetrahydro-2H-pyran-2-yl)oxy)-2-((S)-1-(((2R,3R,4R,5R,6R)-5-hydroxy-3,4-dimethoxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)propan-2-yl)-10-(((2S,3R,4R,6R)-3-hydroxy-4-(methoxyamino)-6-methyltetrahydro-2H-pyran-2-yl)oxy)-3,5,7,9,11,13-hexamethyl-7-(((2-((2-nitrophenyl)sulfonamido)ethyl)carbamoyl)oxy)-6,14-dioxooxacyclotetradecan-4-yl 3-methylbutanoate × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；PEG2000MMES, KSCN
|
Resolution 3.30 Å R-free 0.238 |
| 7AZO 70S thermus thermophilus ribosome with bound antibiotic lead SEQ-977 Deposited 2020-11-17 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain L34B
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 340 OHX osmium (III) hexammine × 301 K POTASSIUM ION × 128 SJE (2R,3S,4R,5R,7S,9S,10S,11R,12S,13R)-12-(((2R,4R,5S,6S)-4,5-dihydroxy-4,6-dimethyltetrahydro-2H-pyran-2-yl)oxy)-2-((S)-1-(((2R,3R,4R,5R,6R)-5-hydroxy-3,4-dimethoxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)propan-2-yl)-10-(((2S,3R,4R,6R)-3-hydroxy-4-(methoxyamino)-6-methyltetrahydro-2H-pyran-2-yl)oxy)-3,5,7,9,11,13-hexamethyl-7-(((2-((2-nitrophenyl)sulfonamido)ethyl)carbamoyl)oxy)-6,14-dioxooxacyclotetradecan-4-yl 3-methylbutanoate × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；PEG2000MMES, KSCN
|
Resolution 3.30 Å R-free 0.238 |
| 7AZS 70S thermus thermophilus ribosome with bound antibiotic lead SEQ-569 Deposited 2020-11-17 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain L34A
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 450 K POTASSIUM ION × 159 OHX osmium (III) hexammine × 304 SJH (2R,3S,4R,5R,7S,9S,10S,11R,12S,13R)-12-(((2R,4R,5S,6S)-4,5-dihydroxy-4,6-dimethyltetrahydro-2H-pyran-2-yl)oxy)-2-((S)-1-(((2R,3R,4R,5R,6R)-5-hydroxy-3,4-dimethoxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)propan-2-yl)-10-(((2S,3R,6R,E)-3-hydroxy-4-(methoxyimino)-6-methyltetrahydro-2H-pyran-2-yl)oxy)-3,5,7,9,11,13-hexamethyl-7-(((2-(2-methyl-5-nitro-1H-imidazol-1-yl)ethyl)carbamoyl)oxy)-6,14-dioxooxacyclotetradecan-4-yl 3-methylbutanoate × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；PEGMMES 2000, KSCN, Tris-HAc
|
Resolution 3.10 Å R-free 0.275 |
| 7AZS 70S thermus thermophilus ribosome with bound antibiotic lead SEQ-569 Deposited 2020-11-17 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain L34B
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 340 K POTASSIUM ION × 129 OHX osmium (III) hexammine × 294 SJH (2R,3S,4R,5R,7S,9S,10S,11R,12S,13R)-12-(((2R,4R,5S,6S)-4,5-dihydroxy-4,6-dimethyltetrahydro-2H-pyran-2-yl)oxy)-2-((S)-1-(((2R,3R,4R,5R,6R)-5-hydroxy-3,4-dimethoxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)propan-2-yl)-10-(((2S,3R,6R,E)-3-hydroxy-4-(methoxyimino)-6-methyltetrahydro-2H-pyran-2-yl)oxy)-3,5,7,9,11,13-hexamethyl-7-(((2-(2-methyl-5-nitro-1H-imidazol-1-yl)ethyl)carbamoyl)oxy)-6,14-dioxooxacyclotetradecan-4-yl 3-methylbutanoate × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；PEGMMES 2000, KSCN, Tris-HAc
|
Resolution 3.10 Å R-free 0.275 |
| 7JQL Crystal structure of the Thermus thermophilus 70S ribosome in complex with Bac7-001, mRNA, and deacylated P-site tRNA at 3.00A resolution Deposited 2020-08-11 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1440 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 3.00 Å R-free 0.285 |
| 7JQL Crystal structure of the Thermus thermophilus 70S ribosome in complex with Bac7-001, mRNA, and deacylated P-site tRNA at 3.00A resolution Deposited 2020-08-11 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1108 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 3.00 Å R-free 0.285 |
| 7JQM Crystal structure of the Thermus thermophilus 70S ribosome in complex with Bac7-002, mRNA, and deacylated P-site tRNA at 3.05A resolution Deposited 2020-08-11 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1174 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 3.05 Å R-free 0.283 |
| 7JQM Crystal structure of the Thermus thermophilus 70S ribosome in complex with Bac7-002, mRNA, and deacylated P-site tRNA at 3.05A resolution Deposited 2020-08-11 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 835 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 3.05 Å R-free 0.283 |
| 7LH5 Crystal structure of the Thermus thermophilus 70S ribosome in complex with plazomicin, mRNA and tRNAs Deposited 2021-01-21 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain B7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 743 EDS (2S)-4-amino-N-[(1R,2S,3S,4R,5S)-5-amino-4-{[(2S,3R)-3-amino-6-{[(2-hydroxyethyl)amino]methyl}-3,4-dihydro-2H-pyran-2-y l]oxy}-2-{[3-deoxy-4-C-methyl-3-(methylamino)-beta-L-arabinopyranosyl]oxy}-3-hydroxycyclohexyl]-2-hydroxybutanamide × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294.15 K；100 mM TRIS-HCl, pH 7.6,
3-3.2% (w/v) PEG 20K,
7-12% (v/v) MPD,
100-200 mM arginine,
0.5 mM Beta-mercaptoethanol
|
Resolution 3.27 Å R-free 0.277 |
| 7LH5 Crystal structure of the Thermus thermophilus 70S ribosome in complex with plazomicin, mRNA and tRNAs Deposited 2021-01-21 | Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 633 EDS (2S)-4-amino-N-[(1R,2S,3S,4R,5S)-5-amino-4-{[(2S,3R)-3-amino-6-{[(2-hydroxyethyl)amino]methyl}-3,4-dihydro-2H-pyran-2-y l]oxy}-2-{[3-deoxy-4-C-methyl-3-(methylamino)-beta-L-arabinopyranosyl]oxy}-3-hydroxycyclohexyl]-2-hydroxybutanamide × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294.15 K；100 mM TRIS-HCl, pH 7.6,
3-3.2% (w/v) PEG 20K,
7-12% (v/v) MPD,
100-200 mM arginine,
0.5 mM Beta-mercaptoethanol
|
Resolution 3.27 Å R-free 0.277 |
| 7MD7 Crystal structure of the Thermus thermophilus 70S ribosome in complex with triphenylphosphonium analog of chloramphenicol CAM-C4-TPP and protein Y (YfiA) at 2.80A resolution Deposited 2021-04-03 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1514 YXM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-5-(triphenyl-lambda~5~-phosphanyl)pentanamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.268 |
| 7MD7 Crystal structure of the Thermus thermophilus 70S ribosome in complex with triphenylphosphonium analog of chloramphenicol CAM-C4-TPP and protein Y (YfiA) at 2.80A resolution Deposited 2021-04-03 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1040 YXM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-5-(triphenyl-lambda~5~-phosphanyl)pentanamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.268 |
| 7RQ8 Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAs, and aminoacylated P-site tRNA at 2.50A resolution Deposited 2021-08-06 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1567 6IF methyl 7-chloro-6,7,8-trideoxy-6-{[(4S,5aS,8S,8aR)-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carbonyl]amino}-1-thio-L-threo-alpha-D-galacto-octopyranoside × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.268 |
| 7RQ8 Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAs, and aminoacylated P-site tRNA at 2.50A resolution Deposited 2021-08-06 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1002 6IF methyl 7-chloro-6,7,8-trideoxy-6-{[(4S,5aS,8S,8aR)-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carbonyl]amino}-1-thio-L-threo-alpha-D-galacto-octopyranoside × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.268 |
| 7RQ9 Crystal structure of the A2058-dimethylated Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAs, and aminoacylated P-site tRNA at 2.60A resolution Deposited 2021-08-06 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1544 K POTASSIUM ION × 1 6IF methyl 7-chloro-6,7,8-trideoxy-6-{[(4S,5aS,8S,8aR)-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carbonyl]amino}-1-thio-L-threo-alpha-D-galacto-octopyranoside × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.298 |
| 7RQ9 Crystal structure of the A2058-dimethylated Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAs, and aminoacylated P-site tRNA at 2.60A resolution Deposited 2021-08-06 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1203 K POTASSIUM ION × 1 6IF methyl 7-chloro-6,7,8-trideoxy-6-{[(4S,5aS,8S,8aR)-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carbonyl]amino}-1-thio-L-threo-alpha-D-galacto-octopyranoside × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.298 |
| 7RQA Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site MTI-tripeptidyl-tRNA analog ACCA-ITM at 2.40A resolution Deposited 2021-08-06 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1495 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.40 Å R-free 0.289 |
| 7RQA Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site MTI-tripeptidyl-tRNA analog ACCA-ITM at 2.40A resolution Deposited 2021-08-06 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1016 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.40 Å R-free 0.289 |
| 7RQB Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site MAI-tripeptidyl-tRNA analog ACCA-IAM at 2.45A resolution Deposited 2021-08-06 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1468 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.45 Å R-free 0.244 |
| 7RQB Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site MAI-tripeptidyl-tRNA analog ACCA-IAM at 2.45A resolution Deposited 2021-08-06 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1000 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.45 Å R-free 0.244 |
| 7RQC Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site MFI-tripeptidyl-tRNA analog ACCA-IFM at 2.50A resolution Deposited 2021-08-06 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1490 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.278 |
| 7RQC Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site MFI-tripeptidyl-tRNA analog ACCA-IFM at 2.50A resolution Deposited 2021-08-06 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1008 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.278 |
| 7RQD Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site deacylated tRNA analog CACCA, P-site MTI-tripeptidyl-tRNA analog ACCA-ITM, and chloramphenicol at 2.50A resolution Deposited 2021-08-06 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1482 CLM CHLORAMPHENICOL × 1 ARG ARGININE × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.280 |
| 7RQD Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site deacylated tRNA analog CACCA, P-site MTI-tripeptidyl-tRNA analog ACCA-ITM, and chloramphenicol at 2.50A resolution Deposited 2021-08-06 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1004 CLM CHLORAMPHENICOL × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.280 |
| 7RQE Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site deacylated tRNA analog CACCA, P-site MAI-tripeptidyl-tRNA analog ACCA-IAM, and chloramphenicol at 2.40A resolution Deposited 2021-08-06 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1474 CLM CHLORAMPHENICOL × 1 ARG ARGININE × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.40 Å R-free 0.269 |
| 7RQE Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site deacylated tRNA analog CACCA, P-site MAI-tripeptidyl-tRNA analog ACCA-IAM, and chloramphenicol at 2.40A resolution Deposited 2021-08-06 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1019 CLM CHLORAMPHENICOL × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.40 Å R-free 0.269 |
| 7U2H Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Gly-NH-tRNAgly, aminoacylated P-site fMet-NH-tRNAmet, and deacylated E-site tRNAgly at 2.55A resolution Deposited 2022-02-24 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1547 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.263 |
| 7U2H Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Gly-NH-tRNAgly, aminoacylated P-site fMet-NH-tRNAmet, and deacylated E-site tRNAgly at 2.55A resolution Deposited 2022-02-24 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1199 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.263 |
| 7U2I Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Gly-NH-tRNAgly, aminoacylated P-site fMet-NH-tRNAmet, deacylated E-site tRNAgly, and chloramphenicol at 2.55A resolution Deposited 2022-02-24 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1547 K POTASSIUM ION × 1 CLM CHLORAMPHENICOL × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.268 |
| 7U2I Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Gly-NH-tRNAgly, aminoacylated P-site fMet-NH-tRNAmet, deacylated E-site tRNAgly, and chloramphenicol at 2.55A resolution Deposited 2022-02-24 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1196 K POTASSIUM ION × 1 CLM CHLORAMPHENICOL × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.268 |
| 7U2J Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Gly-NH-tRNAgly, peptidyl P-site fMAC-NH-tRNAmet, deacylated E-site tRNAgly, and chloramphenicol at 2.55A resolution Deposited 2022-02-24 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1530 K POTASSIUM ION × 1 CLM CHLORAMPHENICOL × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.256 |
| 7U2J Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Gly-NH-tRNAgly, peptidyl P-site fMAC-NH-tRNAmet, deacylated E-site tRNAgly, and chloramphenicol at 2.55A resolution Deposited 2022-02-24 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1121 K POTASSIUM ION × 1 CLM CHLORAMPHENICOL × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.256 |
| 8CVJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMSEAC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.40A resolution Deposited 2022-05-18 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1545 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.40 Å R-free 0.295 |
| 8CVJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMSEAC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.40A resolution Deposited 2022-05-18 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1217 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.40 Å R-free 0.295 |
| 8CVK Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMRC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2022-05-18 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1542 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.284 |
| 8CVK Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMRC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2022-05-18 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1226 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.284 |
| 8CVL Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMTHSMRC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.30A resolution Deposited 2022-05-18 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1534 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.30 Å R-free 0.281 |
| 8CVL Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMTHSMRC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.30A resolution Deposited 2022-05-18 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1189 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.30 Å R-free 0.281 |
| 8EKB Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, deacylated P-site tRNAmet, and thermorubin at 2.70A resolution Deposited 2022-09-20 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1469 K POTASSIUM ION × 1 T8B Thermorubin × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.257 |
| 8EKB Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, deacylated P-site tRNAmet, and thermorubin at 2.70A resolution Deposited 2022-09-20 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 994 K POTASSIUM ION × 1 T8B Thermorubin × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.257 |
| 8EV6 Crystal structure of the Thermus thermophilus 70S ribosome in complex with amikacin, mRNA, and A-, P-, and E-site tRNAs Deposited 2022-10-19 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1382 AKN (2S)-N-[(1R,2S,3S,4R,5S)-4-[(2R,3R,4S,5S,6R)-6-(aminomethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-5-azanyl-2-[(2S,3R,4S,5S ,6R)-4-azanyl-6-(hydroxymethyl)-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-cyclohexyl]-4-azanyl-2-oxidanyl-butanamide × 5 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.15 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.9% PEG-20K, 9-10.5% MPD, 0.5 mM BME
|
Resolution 2.95 Å R-free 0.272 |
| 8EV6 Crystal structure of the Thermus thermophilus 70S ribosome in complex with amikacin, mRNA, and A-, P-, and E-site tRNAs Deposited 2022-10-19 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 951 AKN (2S)-N-[(1R,2S,3S,4R,5S)-4-[(2R,3R,4S,5S,6R)-6-(aminomethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-5-azanyl-2-[(2S,3R,4S,5S ,6R)-4-azanyl-6-(hydroxymethyl)-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-cyclohexyl]-4-azanyl-2-oxidanyl-butanamide × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.15 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.9% PEG-20K, 9-10.5% MPD, 0.5 mM BME
|
Resolution 2.95 Å R-free 0.272 |
| 8EV7 Crystal structure of the Thermus thermophilus 70S ribosome in complex with kanamycin, mRNA, and A-, P-, and E-site tRNAs Deposited 2022-10-19 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1416 KAN KANAMYCIN A × 4 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.15 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.9% PEG-20K, 9-10.5% MPD, 0.5 mM BME
|
Resolution 2.89 Å R-free 0.263 |
| 8EV7 Crystal structure of the Thermus thermophilus 70S ribosome in complex with kanamycin, mRNA, and A-, P-, and E-site tRNAs Deposited 2022-10-19 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 843 KAN KANAMYCIN A × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.15 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.9% PEG-20K, 9-10.5% MPD, 0.5 mM BME
|
Resolution 2.89 Å R-free 0.263 |
| 8FC1 Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and erythromycin at 2.50A resolution Deposited 2022-12-01 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1505 HGR Hygromycin A × 1 ERY ERYTHROMYCIN A × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.246 |
| 8FC1 Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and erythromycin at 2.50A resolution Deposited 2022-12-01 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1002 HGR Hygromycin A × 1 ERY ERYTHROMYCIN A × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.246 |
| 8FC2 Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and azithromycin at 2.50A resolution Deposited 2022-12-01 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1491 HGR Hygromycin A × 1 ZIT AZITHROMYCIN × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.250 |
| 8FC2 Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and azithromycin at 2.50A resolution Deposited 2022-12-01 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1007 HGR Hygromycin A × 1 ZIT AZITHROMYCIN × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.250 |
| 8FC3 Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and telithromycin at 2.60A resolution Deposited 2022-12-01 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1486 HGR Hygromycin A × 1 TEL TELITHROMYCIN × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.245 |
| 8FC3 Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and telithromycin at 2.60A resolution Deposited 2022-12-01 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1011 HGR Hygromycin A × 1 TEL TELITHROMYCIN × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.245 |
| 8FC4 Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and erythromycin at 2.45A resolution Deposited 2022-12-01 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1487 HGR Hygromycin A × 1 ERY ERYTHROMYCIN A × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.45 Å R-free 0.264 |
| 8FC4 Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and erythromycin at 2.45A resolution Deposited 2022-12-01 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 928 HGR Hygromycin A × 1 ERY ERYTHROMYCIN A × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.45 Å R-free 0.264 |
| 8FC5 Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and azithromycin at 2.65A resolution Deposited 2022-12-01 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1500 HGR Hygromycin A × 1 ZIT AZITHROMYCIN × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.65 Å R-free 0.236 |
| 8FC5 Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and azithromycin at 2.65A resolution Deposited 2022-12-01 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 966 HGR Hygromycin A × 1 ZIT AZITHROMYCIN × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.65 Å R-free 0.236 |
| 8FC6 Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and telithromycin at 2.35A resolution Deposited 2022-12-01 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1487 HGR Hygromycin A × 1 TEL TELITHROMYCIN × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.35 Å R-free 0.248 |
| 8FC6 Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and telithromycin at 2.35A resolution Deposited 2022-12-01 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1009 HGR Hygromycin A × 1 TEL TELITHROMYCIN × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.35 Å R-free 0.248 |
| 8FOM Crystal structure of tRNA^Lys(SUU) bound to UAA codon in the ribosomal P site Deposited 2023-01-02 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 401 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
|
Resolution 3.58 Å R-free 0.255 |
| 8FOM Crystal structure of tRNA^Lys(SUU) bound to UAA codon in the ribosomal P site Deposited 2023-01-02 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 441 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
|
Resolution 3.58 Å R-free 0.255 |
| 8FON Crystal structure of tRNA^Lys(SUU) bound to AUA codon in the ribosomal P site Deposited 2023-01-02 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain R7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 586 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
|
Resolution 3.64 Å R-free 0.278 |
| 8FON Crystal structure of tRNA^Lys(SUU) bound to AUA codon in the ribosomal P site Deposited 2023-01-02 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Y7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 653 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
|
Resolution 3.64 Å R-free 0.278 |
| 8G29 Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.55A resolution Deposited 2023-02-03 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1559 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.252 |
| 8G29 Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.55A resolution Deposited 2023-02-03 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 897 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.252 |
| 8G2A Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-tRNAphe, peptidyl P-site fMTHSMRC-tRNAmet, and deacylated E-site tRNAphe at 2.45A resolution Deposited 2023-02-03 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1573 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.45 Å R-free 0.249 |
| 8G2A Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-tRNAphe, peptidyl P-site fMTHSMRC-tRNAmet, and deacylated E-site tRNAphe at 2.45A resolution Deposited 2023-02-03 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1176 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.45 Å R-free 0.249 |
| 8G2B Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAphe, and aminoacylated P-site fMet-tRNAmet at 2.55A resolution Deposited 2023-02-03 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1532 6IF methyl 7-chloro-6,7,8-trideoxy-6-{[(4S,5aS,8S,8aR)-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carbonyl]amino}-1-thio-L-threo-alpha-D-galacto-octopyranoside × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.262 |
| 8G2B Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAphe, and aminoacylated P-site fMet-tRNAmet at 2.55A resolution Deposited 2023-02-03 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1164 6IF methyl 7-chloro-6,7,8-trideoxy-6-{[(4S,5aS,8S,8aR)-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carbonyl]amino}-1-thio-L-threo-alpha-D-galacto-octopyranoside × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.262 |
| 8G2C Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with tylosin, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.65A resolution Deposited 2023-02-03 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1533 K POTASSIUM ION × 1 TYK TYLOSIN × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.65 Å R-free 0.249 |
| 8G2C Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with tylosin, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.65A resolution Deposited 2023-02-03 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1135 TYK TYLOSIN × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.65 Å R-free 0.249 |
| 8G2D Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with tylosin, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.70A resolution Deposited 2023-02-03 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1554 TYK TYLOSIN × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.277 |
| 8G2D Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with tylosin, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.70A resolution Deposited 2023-02-03 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1235 TYK TYLOSIN × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.277 |
| 8T8B Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site formyl-MAI-tripeptidyl-tRNA analog ACCA-IAMf at 2.65A resolution Deposited 2023-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 102 PDB declaration: 112-meric（112） Consistent with all polymers |
Chain 17
1–49（49 aa）
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 2428 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 6 ARG ARGININE × 2 ZN ZINC ION × 12 SF4 IRON/SULFUR CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.65 Å R-free 0.257 |
| 8T8C Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site formyl-MFI-tripeptidyl-tRNA analog ACCA-IFMf at 2.60A resolution Deposited 2023-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 102 PDB declaration: 112-meric（112） Consistent with all polymers |
Chain 17
1–49（49 aa）
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 2490 ARG ARGININE × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 7 ZN ZINC ION × 12 SF4 IRON/SULFUR CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.269 |
| 8UD6 Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.70A resolution Deposited 2023-09-28 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1487 K POTASSIUM ION × 1 WC9 (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-13-oxa-2-thiabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.241 |
| 8UD6 Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.70A resolution Deposited 2023-09-28 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1084 K POTASSIUM ION × 1 WC9 (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-13-oxa-2-thiabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.241 |
| 8UD7 Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.70A resolution Deposited 2023-09-28 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1521 WC9 (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-13-oxa-2-thiabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.242 |
| 8UD7 Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.70A resolution Deposited 2023-09-28 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1143 WC9 (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-13-oxa-2-thiabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.242 |
| 8UD8 Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.70A resolution Deposited 2023-09-28 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1546 K POTASSIUM ION × 1 WC9 (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-13-oxa-2-thiabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.261 |
| 8UD8 Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.70A resolution Deposited 2023-09-28 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1199 K POTASSIUM ION × 1 WC9 (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-13-oxa-2-thiabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.261 |
| 8UVR Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with spectinomycin, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.60A resolution Deposited 2023-11-03 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1576 K POTASSIUM ION × 1 ZN ZINC ION × 6 SCM SPECTINOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.265 |
| 8UVR Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with spectinomycin, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.60A resolution Deposited 2023-11-03 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1250 K POTASSIUM ION × 1 ZN ZINC ION × 6 SCM SPECTINOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.265 |
| 8UVS Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with spectinomycin derivative 2694, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.75A resolution Deposited 2023-11-03 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1611 ZN ZINC ION × 6 Y7K (2R,4R,4aS,5aR,6S,7S,8R,9S,9aR,10aS)-2-methyl-6,8-bis(methylamino)-4-({[2-(oxan-4-yl)ethyl]amino}methyl)octahydro-2H-pyrano[2,3-b][1,4]benzodioxine-4,4a,7,9(10aH)-tetrol × 1 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.75 Å R-free 0.261 |
| 8UVS Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with spectinomycin derivative 2694, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.75A resolution Deposited 2023-11-03 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1250 ZN ZINC ION × 6 Y7K (2R,4R,4aS,5aR,6S,7S,8R,9S,9aR,10aS)-2-methyl-6,8-bis(methylamino)-4-({[2-(oxan-4-yl)ethyl]amino}methyl)octahydro-2H-pyrano[2,3-b][1,4]benzodioxine-4,4a,7,9(10aH)-tetrol × 1 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.75 Å R-free 0.261 |
| 8VTU Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-66, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.40A resolution Deposited 2024-01-27 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1568 K POTASSIUM ION × 1 A1AEZ 1-cyclopropyl-7-[(4-{[3-({(3aR,4R,7R,8S,9S,10R,11R,13R,14E,15S,15aR)-10-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-4-ethyl-11-methoxy-3a,7,9,11,13,15-hexamethyl-14-[({3-[5-(methylcarbamoyl)pyridin-3-yl]prop-2-yn-1-yl}oxy)imino]-2,6-dioxododecahydro-2H,4H-[1,3]dioxolo[4,5-c]oxacyclotetradecin-8-yl}oxy)-3-oxopropyl]amino}butyl)amino]-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.40 Å R-free 0.260 |
| 8VTU Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-66, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.40A resolution Deposited 2024-01-27 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1187 K POTASSIUM ION × 1 A1AEZ 1-cyclopropyl-7-[(4-{[3-({(3aR,4R,7R,8S,9S,10R,11R,13R,14E,15S,15aR)-10-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-4-ethyl-11-methoxy-3a,7,9,11,13,15-hexamethyl-14-[({3-[5-(methylcarbamoyl)pyridin-3-yl]prop-2-yn-1-yl}oxy)imino]-2,6-dioxododecahydro-2H,4H-[1,3]dioxolo[4,5-c]oxacyclotetradecin-8-yl}oxy)-3-oxopropyl]amino}butyl)amino]-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.40 Å R-free 0.260 |
| 8VTV Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-91, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.55A resolution Deposited 2024-01-27 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1554 K POTASSIUM ION × 1 A1AE0 1-cyclopropyl-7-{7-[({[(3aR,4R,7R,8S,9S,10R,11R,13R,14E,15S,15aR)-10-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-4-ethyl-11-methoxy-14-(methoxyimino)-3a,7,9,11,13,15-hexamethyl-2,6-dioxododecahydro-2H,4H-[1,3]dioxolo[4,5-c]oxacyclotetradecin-8-yl]oxy}carbonyl)amino]hept-1-yn-1-yl}-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.262 |
| 8VTV Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-91, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.55A resolution Deposited 2024-01-27 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1218 K POTASSIUM ION × 1 A1AE0 1-cyclopropyl-7-{7-[({[(3aR,4R,7R,8S,9S,10R,11R,13R,14E,15S,15aR)-10-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-4-ethyl-11-methoxy-14-(methoxyimino)-3a,7,9,11,13,15-hexamethyl-2,6-dioxododecahydro-2H,4H-[1,3]dioxolo[4,5-c]oxacyclotetradecin-8-yl]oxy}carbonyl)amino]hept-1-yn-1-yl}-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.262 |
| 8VTW Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-128 and protein Y at 2.35A resolution Deposited 2024-01-27 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1450 A1AE1 1-cyclopropyl-7-(4-{4-[({[(3aR,4R,7R,8S,9S,10R,11R,13R,14E,15S,15aR)-10-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-4-ethyl-11-methoxy-14-(methoxyimino)-3a,7,9,11,13,15-hexamethyl-2,6-dioxododecahydro-2H,4H-[1,3]dioxolo[4,5-c]oxacyclotetradecin-8-yl]oxy}carbonyl)amino]butyl}piperazin-1-yl)-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (non-preferred name) × 1 ARG ARGININE × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.35 Å R-free 0.243 |
| 8VTW Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-128 and protein Y at 2.35A resolution Deposited 2024-01-27 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 984 A1AE1 1-cyclopropyl-7-(4-{4-[({[(3aR,4R,7R,8S,9S,10R,11R,13R,14E,15S,15aR)-10-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-4-ethyl-11-methoxy-14-(methoxyimino)-3a,7,9,11,13,15-hexamethyl-2,6-dioxododecahydro-2H,4H-[1,3]dioxolo[4,5-c]oxacyclotetradecin-8-yl]oxy}carbonyl)amino]butyl}piperazin-1-yl)-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (non-preferred name) × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.35 Å R-free 0.243 |
| 8VTX Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with macrolone MCX-128, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.40A resolution Deposited 2024-01-27 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1548 K POTASSIUM ION × 1 A1AE1 1-cyclopropyl-7-(4-{4-[({[(3aR,4R,7R,8S,9S,10R,11R,13R,14E,15S,15aR)-10-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-4-ethyl-11-methoxy-14-(methoxyimino)-3a,7,9,11,13,15-hexamethyl-2,6-dioxododecahydro-2H,4H-[1,3]dioxolo[4,5-c]oxacyclotetradecin-8-yl]oxy}carbonyl)amino]butyl}piperazin-1-yl)-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.40 Å R-free 0.260 |
| 8VTX Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with macrolone MCX-128, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.40A resolution Deposited 2024-01-27 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1185 K POTASSIUM ION × 1 A1AE1 1-cyclopropyl-7-(4-{4-[({[(3aR,4R,7R,8S,9S,10R,11R,13R,14E,15S,15aR)-10-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-4-ethyl-11-methoxy-14-(methoxyimino)-3a,7,9,11,13,15-hexamethyl-2,6-dioxododecahydro-2H,4H-[1,3]dioxolo[4,5-c]oxacyclotetradecin-8-yl]oxy}carbonyl)amino]butyl}piperazin-1-yl)-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.40 Å R-free 0.260 |
| 8VTY Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with ciprofloxacin and protein Y at 2.60A resolution Deposited 2024-01-27 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1526 CPF 1-CYCLOPROPYL-6-FLUORO-4-OXO-7-PIPERAZIN-1-YL-1,4-DIHYDROQUINOLINE-3-CARBOXYLIC ACID × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.241 |
| 8VTY Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with ciprofloxacin and protein Y at 2.60A resolution Deposited 2024-01-27 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1021 CPF 1-CYCLOPROPYL-6-FLUORO-4-OXO-7-PIPERAZIN-1-YL-1,4-DIHYDROQUINOLINE-3-CARBOXYLIC ACID × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.241 |
| 8WV1 Ambient Temperature Structure of 50S Ribosomal Subunit from Thermus Thermophilus Deposited 2023-10-22 | Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 62-meric（62） Consistent with all polymers |
Chain 2
1–49（49 aa）
Chain 7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 3807 ZN ZINC ION × 10 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；277 K；20% w/v PEG 3350
200 mM ammonium sulfate tribasic
|
Resolution 3.99 Å R-free 0.362 |
| 9B00 Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with berberine analog of chloramphenicol CAM-BER, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.80A resolution Deposited 2024-03-11 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1524 K POTASSIUM ION × 1 A1AIX 13-(2-{[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]amino}-2-oxoethyl)-9,10-dimethoxy-5,6-dihydro-2H-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.284 |
| 9B00 Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with berberine analog of chloramphenicol CAM-BER, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.80A resolution Deposited 2024-03-11 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1199 K POTASSIUM ION × 1 A1AIX 13-(2-{[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]amino}-2-oxoethyl)-9,10-dimethoxy-5,6-dihydro-2H-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.284 |
| 9D0G Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with O-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2024-08-07 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1553 K POTASSIUM ION × 1 A1A1F (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1S,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-2,13-dioxabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.265 |
| 9D0G Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with O-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2024-08-07 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1239 K POTASSIUM ION × 1 A1A1F (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1S,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-2,13-dioxabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.265 |
| 9D0H Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with C-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2024-08-07 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1549 K POTASSIUM ION × 1 A1A1J (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,2R,3R,4Z,9R,10R,11R,12R)-10,11,12-trihydroxy-3-methyl-13-oxabicyclo[7.3.1]tridec-4-en-2-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.266 |
| 9D0H Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with C-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2024-08-07 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1241 K POTASSIUM ION × 1 A1A1J (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,2R,3R,4Z,9R,10R,11R,12R)-10,11,12-trihydroxy-3-methyl-13-oxabicyclo[7.3.1]tridec-4-en-2-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.266 |
| 9D0I Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Se-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.45A resolution Deposited 2024-08-07 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1551 K POTASSIUM ION × 1 A1A1K (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-13-oxa-2-selenabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.45 Å R-free 0.265 |
| 9D0I Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Se-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.45A resolution Deposited 2024-08-07 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1244 K POTASSIUM ION × 1 A1A1K (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-13-oxa-2-selenabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.45 Å R-free 0.265 |
| 9D0J Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with BT-33, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2024-08-07 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1475 A1A1L (4S,5aS,8S,8aR)-N-[(1R,4R,6Z,8R,9R,10R,11R,12S,13R)-4-fluoro-11,12,13-trihydroxy-8-methyl-14-oxa-2-thiabicyclo[8.3.1]tetradec-6-en-9-yl]-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.260 |
| 9D0J Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with BT-33, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2024-08-07 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1089 A1A1L (4S,5aS,8S,8aR)-N-[(1R,4R,6Z,8R,9R,10R,11R,12S,13R)-4-fluoro-11,12,13-trihydroxy-8-methyl-14-oxa-2-thiabicyclo[8.3.1]tetradec-6-en-9-yl]-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.260 |
| 9D7R Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Fva1 antimicrobial peptide, mRNA, A-site release factor 1, and deacylated P-site and E-site tRNAphe at 2.70A resolution Deposited 2024-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1123 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.275 |
| 9D7R Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Fva1 antimicrobial peptide, mRNA, A-site release factor 1, and deacylated P-site and E-site tRNAphe at 2.70A resolution Deposited 2024-08-17 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 885 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.275 |
| 9D7S Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Api antimicrobial peptide, mRNA, A-site release factor 1, and deacylated P-site and E-site tRNAphe at 2.85A resolution Deposited 2024-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1101 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.85 Å R-free 0.268 |
| 9D7S Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Api antimicrobial peptide, mRNA, A-site release factor 1, and deacylated P-site and E-site tRNAphe at 2.85A resolution Deposited 2024-08-17 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 891 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.85 Å R-free 0.268 |
| 9D7T Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Api137 antimicrobial peptide, mRNA, A-site release factor 1, and deacylated P-site and E-site tRNAphe at 2.70A resolution Deposited 2024-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1127 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.278 |
| 9D7T Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Api137 antimicrobial peptide, mRNA, A-site release factor 1, and deacylated P-site and E-site tRNAphe at 2.70A resolution Deposited 2024-08-17 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 915 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.278 |
| 9DFC Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with lasso peptide lariocidin, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2024-08-29 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1554 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.265 |
| 9DFC Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with lasso peptide lariocidin, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2024-08-29 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1250 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.50 Å R-free 0.265 |
| 9DFD Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with lasso peptide lariocidin B, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.60A resolution Deposited 2024-08-29 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1554 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.266 |
| 9DFD Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with lasso peptide lariocidin B, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.60A resolution Deposited 2024-08-29 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1250 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.266 |
| 9DFE Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with lasso peptide lariocidin and protein Y at 2.60A resolution Deposited 2024-08-29 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1496 ARG ARGININE × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.252 |
| 9DFE Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with lasso peptide lariocidin and protein Y at 2.60A resolution Deposited 2024-08-29 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1024 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.252 |
| 9MTP Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.40A resolution Deposited 2025-01-12 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1538 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.40 Å R-free 0.263 |
| 9MTP Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.40A resolution Deposited 2025-01-12 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1138 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.40 Å R-free 0.263 |
| 9MTQ Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site GGD-mutant Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.55A resolution Deposited 2025-01-12 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1527 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.257 |
| 9MTQ Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site GGD-mutant Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.55A resolution Deposited 2025-01-12 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1151 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.55 Å R-free 0.257 |
| 9MTR Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site GGS-mutant Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.80A resolution Deposited 2025-01-12 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1531 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.264 |
| 9MTR Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site GGS-mutant Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.80A resolution Deposited 2025-01-12 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1118 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.264 |
| 9MTS Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-unmodified Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.70A resolution Deposited 2025-01-12 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1465 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.262 |
| 9MTS Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-unmodified Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.70A resolution Deposited 2025-01-12 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1124 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.262 |
| 9MTT Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site deacylated-tRNAcys at 2.60A resolution Deposited 2025-01-12 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1539 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.271 |
| 9MTT Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site deacylated-tRNAcys at 2.60A resolution Deposited 2025-01-12 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1148 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.271 |
| 9NO7 Cryo-EM structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys at 2.13A resolution Deposited 2025-03-08 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 7
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1685 K POTASSIUM ION × 52 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6；5 mM HEPES-KOH, 50 mM KCl, 10 mM NH4Cl, 10 mM Mg(CH3COO)2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.13 Å |
| 9O3H Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolide erythromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMRC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.65A resolution Deposited 2025-04-07 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1542 K POTASSIUM ION × 1 ERY ERYTHROMYCIN A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.65 Å R-free 0.276 |
| 9O3H Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolide erythromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMRC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.65A resolution Deposited 2025-04-07 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1207 K POTASSIUM ION × 1 ERY ERYTHROMYCIN A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.65 Å R-free 0.276 |
| 9O3I Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with ketolide telithromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMRC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.80A resolution Deposited 2025-04-07 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1518 K POTASSIUM ION × 1 TEL TELITHROMYCIN × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.281 |
| 9O3I Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with ketolide telithromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMRC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.80A resolution Deposited 2025-04-07 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1141 K POTASSIUM ION × 1 TEL TELITHROMYCIN × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.80 Å R-free 0.281 |
| 9O3J Crystal structure of the wild-type drug-free Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMRC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.60A resolution Deposited 2025-04-07 | Assembly 1 Protein–RNA Heteromer；Protein × 100 PDB declaration: 114-meric（114） Consistent with all polymers |
Chain 17
1–49（49 aa）
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 2734 K POTASSIUM ION × 2 ZN ZINC ION × 12 SF4 IRON/SULFUR CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.60 Å R-free 0.278 |
| 9O3K Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolide erythromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMAC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.70A resolution Deposited 2025-04-07 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 17
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 1529 K POTASSIUM ION × 1 ERY ERYTHROMYCIN A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.262 |
| 9O3K Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolide erythromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMAC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.70A resolution Deposited 2025-04-07 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 993 K POTASSIUM ION × 1 ERY ERYTHROMYCIN A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.70 Å R-free 0.262 |
| 9O3L Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolide erythromycin, mRNA, deacylated A-site tRNAphe, P-site fMRC-peptidyl-tRNAmet, and deacylated E-site tRNAphe at 2.75A resolution Deposited 2025-04-07 | Assembly 1 Protein–RNA Heteromer；Protein × 100 PDB declaration: 114-meric（114） Consistent with all polymers |
Chain 17
1–49（49 aa）
Chain 27
1–49（49 aa）
|
Not recorded | MG MAGNESIUM ION × 2713 K POTASSIUM ION × 2 ERY ERYTHROMYCIN A × 2 ZN ZINC ION × 12 SF4 IRON/SULFUR CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
|
Resolution 2.75 Å R-free 0.282 |