Current Protein Identity:Q03290
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 5OQJ STRUCTURE OF YEAST TRANSCRIPTION PRE-INITIATION COMPLEX WITH TFIIH Deposited 2017-08-11 | Assembly 1 Protein–DNA Heteromer;Protein × 29 PDB declaration: 31-meric(31) Consistent with all polymers |
Chain 3
1–321(321 aa)
|
Not recorded | ZN ZINC ION × 16 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE;GRIDS WERE BLOTTED FOR 8 S AND PLUNGE-FROZEN IN LIQUID ETHANE.
|
Resolution 4.70 Å |
| 5OQM STRUCTURE OF YEAST TRANSCRIPTION PRE-INITIATION COMPLEX WITH TFIIH AND CORE MEDIATOR Deposited 2017-08-13 | Assembly 1 Insufficient information Heteromer;Protein × 44 PDB declaration: 46-meric(46) Consistent with all polymers |
Chain 3
1–321(321 aa)
|
Not recorded | ZN ZINC ION × 16 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE;Grids were blotted for 8 s and plunge-frozen in liquid ethane.
|
Resolution 5.80 Å |
| 6GYM Structure of a yeast closed complex with distorted DNA (CCdist) Deposited 2018-06-30 | Assembly 1 Protein–DNA Heteromer;Protein × 29 PDB declaration: 31-meric(31) Consistent with all polymers |
Chain 3
1–321(321 aa)
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 16 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.70 Å |
| 6XI8 Yeast TFIIK (Kin28/Ccl1/Tfb3) Complex Deposited 2020-06-19 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain C
259–321(63 aa)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 AF3 ALUMINUM FLUORIDE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE;blotted for 2 seconds with Whatman 41 ashless filter paper
|
Resolution 3.64 Å |
| 7KUE CryoEM structure of Yeast TFIIK (Kin28/Ccl1/Tfb3) Complex Deposited 2020-11-24 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain C
1–321(321 aa)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 AF3 ALUMINUM FLUORIDE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE;blotted for 2 seconds with Whatman 41 ashless filter paper
|
Resolution 3.50 Å |
| 7O4I Yeast RNA polymerase II transcription pre-initiation complex with initial transcription bubble Deposited 2021-04-06 | Assembly 1 Protein–DNA Heteromer;Protein × 28 PDB declaration: 30-meric(30) Consistent with all polymers |
Chain 3
1–321(321 aa)
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 17 BEF BERYLLIUM TRIFLUORIDE ION × 1 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 7O4J Yeast RNA polymerase II transcription pre-initiation complex (consensus) Deposited 2021-04-06 | Assembly 1 Protein–DNA Heteromer;Protein × 28 PDB declaration: 30-meric(30) Consistent with all polymers |
Chain 3
1–321(321 aa)
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 17 BEF BERYLLIUM TRIFLUORIDE ION × 1 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7O4K Yeast TFIIH in the contracted state within the pre-initiation complex Deposited 2021-04-06 | Assembly 1 Protein–DNA Heteromer;Protein × 15 PDB declaration: heptadecameric(17) Consistent with all polymers |
Chain 3
1–321(321 aa)
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 10 BEF BERYLLIUM TRIFLUORIDE ION × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 7O4L Yeast TFIIH in the expanded state within the pre-initiation complex Deposited 2021-04-06 | Assembly 1 Protein–DNA Heteromer;Protein × 15 PDB declaration: heptadecameric(17) Consistent with all polymers |
Chain 3
1–321(321 aa)
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 11 BEF BERYLLIUM TRIFLUORIDE ION × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 7O72 Yeast RNA polymerase II transcription pre-initiation complex with closed promoter DNA Deposited 2021-04-12 | Assembly 1 Protein–DNA Heteromer;Protein × 28 PDB declaration: 30-meric(30) Consistent with all polymers |
Chain 3
1–321(321 aa)
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 17 BEF BERYLLIUM TRIFLUORIDE ION × 1 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 7O73 Yeast RNA polymerase II transcription pre-initiation complex with closed distorted promoter DNA Deposited 2021-04-12 | Assembly 1 Protein–DNA Heteromer;Protein × 28 PDB declaration: 30-meric(30) Consistent with all polymers |
Chain 3
1–321(321 aa)
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 17 BEF BERYLLIUM TRIFLUORIDE ION × 1 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 7O75 Yeast RNA polymerase II transcription pre-initiation complex with open promoter DNA Deposited 2021-04-13 | Assembly 1 Protein–DNA Heteromer;Protein × 28 PDB declaration: 30-meric(30) Consistent with all polymers |
Chain 3
1–321(321 aa)
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 17 BEF BERYLLIUM TRIFLUORIDE ION × 1 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 7ZS9 Yeast RNA polymerase II transcription pre-initiation complex with the +1 nucleosome (complex A) Deposited 2022-05-06 | Assembly 1 Protein–DNA Heteromer;Protein × 36 PDB declaration: 38-meric(38) Consistent with all polymers |
Chain 3
1–321(321 aa)
|
Not recorded | ZN ZINC ION × 17 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 7ZSA Yeast RNA polymerase II transcription pre-initiation complex with the +1 nucleosome and NTP (complex B) Deposited 2022-05-06 | Assembly 1 Protein–DNA Heteromer;Protein × 36 PDB declaration: 38-meric(38) Consistent with all polymers |
Chain 3
1–321(321 aa)
|
Not recorded | ZN ZINC ION × 17 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 7ZSB Yeast RNA polymerase II transcription pre-initiation complex with the +1 nucleosome and NTP, complex C Deposited 2022-05-06 | Assembly 1 Protein–DNA Heteromer;Protein × 36 PDB declaration: 38-meric(38) Consistent with all polymers |
Chain 3
1–321(321 aa)
|
Not recorded | ZN ZINC ION × 17 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å |