Current Protein Identity:Q04636
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2GCJ Crystal Structure of the Pob3 middle domain Deposited 2006-03-14 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
220–478(259 aa)
Fragment:Pob3 Middle domain
|
Mutation:Q308K | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.5;298 K;21% PEG 3350, 20% Glycerol, 200mM NaCl, 50mM Ammonium Sulphate, 100mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.55 Å R-free 0.303 |
| 2GCJ Crystal Structure of the Pob3 middle domain Deposited 2006-03-14 | Assembly 2 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain B
220–478(259 aa)
Fragment:Pob3 Middle domain
|
Mutation:Q308K | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.5;298 K;21% PEG 3350, 20% Glycerol, 200mM NaCl, 50mM Ammonium Sulphate, 100mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.55 Å R-free 0.303 |
| 2GCJ Crystal Structure of the Pob3 middle domain Deposited 2006-03-14 | Assembly 3 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain C
220–478(259 aa)
Fragment:Pob3 Middle domain
|
Mutation:Q308K | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.5;298 K;21% PEG 3350, 20% Glycerol, 200mM NaCl, 50mM Ammonium Sulphate, 100mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.55 Å R-free 0.303 |
| 2GCJ Crystal Structure of the Pob3 middle domain Deposited 2006-03-14 | Assembly 4 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain D
220–478(259 aa)
Fragment:Pob3 Middle domain
|
Mutation:Q308K | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.5;298 K;21% PEG 3350, 20% Glycerol, 200mM NaCl, 50mM Ammonium Sulphate, 100mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.55 Å R-free 0.303 |
| 2GCL Structure of the Pob3 Middle domain Deposited 2006-03-14 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
220–478(259 aa)
Fragment:Middle domain
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | CL CHLORIDE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.5;298 K;21% PEG 3350, 20% glycerol, 200mM NaCl, 50mM Ammonium sulphate, 100mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.21 Å R-free 0.264 |
| 2GCL Structure of the Pob3 Middle domain Deposited 2006-03-14 | Assembly 2 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain B
220–478(259 aa)
Fragment:Middle domain
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.5;298 K;21% PEG 3350, 20% glycerol, 200mM NaCl, 50mM Ammonium sulphate, 100mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.21 Å R-free 0.264 |
| 3F5R The crystal structure of a subunit of the heterodimeric FACT complex (Spt16p-Pob3p). Deposited 2008-11-04 | Assembly 1 Protein homooligomer Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
1–168(168 aa)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | CL CHLORIDE ION × 2 FMT FORMIC ACID × 2 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 6.5;293 K;0.1M Bis-tris, 1.5M (NH4)2SO4., pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.192 |
| 4PQ0 Crystal structure of POB3 middle domain at 1.65A Deposited 2014-02-28 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
232–473(242 aa)
Fragment:UNP RESIDUES 232-473
|
Not recorded | MLA MALONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7;287 K;0.1M Sodium malonate pH 7.0, 0.1M Ammonium acetate, 20% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 287K
|
Resolution 1.65 Å R-free 0.248 |
| 8XGC Structure of yeast replisome associated with FACT and histone hexamer, Composite map Deposited 2023-12-15 | Assembly 1 Protein–DNA Heteromer;Protein × 27 PDB declaration: 29-meric(29) Consistent with all polymers |
Chain M
1–552(552 aa)
|
Not recorded | ZN ZINC ION × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |