Current Protein Identity：Q40059
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1TM3 crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59k mutant Deposited 2004-06-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：I20, I45A, I59K | CA CALCIUM ION × 1 NA SODIUM ION × 1 CIT CITRIC ACID × 2 1PE PENTAETHYLENE GLYCOL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 4000, xylitol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.57 Å R-free 0.184 |
| 1TM3 crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59k mutant Deposited 2004-06-10 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：I20, I45A, I59K | CA CALCIUM ION × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 4 1PE PENTAETHYLENE GLYCOL × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 4000, xylitol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.57 Å R-free 0.184 |
| 1TM4 crystal structure of the complex of subtilsin BPN'with chymotrypsin inhibitor 2 M59G mutant Deposited 2004-06-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：I45A,I59G | CA CALCIUM ION × 1 NA SODIUM ION × 1 CIT CITRIC ACID × 2 1PE PENTAETHYLENE GLYCOL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 400, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.183 |
| 1TM4 crystal structure of the complex of subtilsin BPN'with chymotrypsin inhibitor 2 M59G mutant Deposited 2004-06-10 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：I45A,I59G | CA CALCIUM ION × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 4 1PE PENTAETHYLENE GLYCOL × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 400, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.183 |
| 1TM5 crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59A mutant Deposited 2004-06-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–83（62 aa）
|
Mutation：I45A; I59A | CA CALCIUM ION × 1 NA SODIUM ION × 1 CIT CITRIC ACID × 3 1PE PENTAETHYLENE GLYCOL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG monomethyl ether 750, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.45 Å R-free 0.179 |
| 1TM5 crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59A mutant Deposited 2004-06-10 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain I
22–83（62 aa）
|
Mutation：I45A; I59A | CA CALCIUM ION × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 6 1PE PENTAETHYLENE GLYCOL × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG monomethyl ether 750, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.45 Å R-free 0.179 |
| 1TM7 crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59Y mutant Deposited 2004-06-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：I45A, I59Y, | CA CALCIUM ION × 1 NA SODIUM ION × 1 CIT CITRIC ACID × 3 1PE PENTAETHYLENE GLYCOL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 750 monomethyl ether, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.59 Å R-free 0.175 |
| 1TM7 crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59Y mutant Deposited 2004-06-10 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：I45A, I59Y, | CA CALCIUM ION × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 6 1PE PENTAETHYLENE GLYCOL × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 750 monomethyl ether, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.59 Å R-free 0.175 |
| 1TM7 crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59Y mutant Deposited 2004-06-10 | Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：I45A, I59Y, | CA CALCIUM ION × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 6 1PE PENTAETHYLENE GLYCOL × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 750 monomethyl ether, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.59 Å R-free 0.175 |
| 1TMG crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59F mutant Deposited 2004-06-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：I20, initiating Met; I45A, I59F | CA CALCIUM ION × 1 NA SODIUM ION × 1 CIT CITRIC ACID × 3 1PE PENTAETHYLENE GLYCOL × 4 15P POLYETHYLENE GLYCOL (N=34) × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 400, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.67 Å R-free 0.174 |
| 1TO1 crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 Y61A mutant Deposited 2004-06-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：I45 A; I61A | CA CALCIUM ION × 1 NA SODIUM ION × 1 CIT CITRIC ACID × 2 1PE PENTAETHYLENE GLYCOL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 400, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.68 Å R-free 0.182 |
| 1TO2 crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59K, in pH 9 cryosoak Deposited 2004-06-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：I45A; I59K | CA CALCIUM ION × 1 NA SODIUM ION × 1 CIT CITRIC ACID × 2 15P POLYETHYLENE GLYCOL (N=34) × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；277 K；sodium citrate, isopropanol, PEG 4000, xylitol, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.30 Å R-free 0.183 |
| 1Y1K Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 T58A mutant Deposited 2004-11-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：initiating Met, T58A | CA CALCIUM ION × 1 NA SODIUM ION × 1 CIT CITRIC ACID × 3 15P POLYETHYLENE GLYCOL (N=34) × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 2000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.56 Å R-free 0.185 |
| 1Y33 Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 T58P mutant Deposited 2004-11-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：initiating Met, T58P | CA CALCIUM ION × 1 NA SODIUM ION × 1 CIT CITRIC ACID × 3 15P POLYETHYLENE GLYCOL (N=34) × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 2000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.179 |
| 1Y34 Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 E60A mutant Deposited 2004-11-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：initiating Met, E60A | CA CALCIUM ION × 1 NA SODIUM ION × 1 CIT CITRIC ACID × 3 15P POLYETHYLENE GLYCOL (N=34) × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG monomethyl ether 750, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.55 Å R-free 0.180 |
| 1Y34 Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 E60A mutant Deposited 2004-11-23 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：initiating Met, E60A | CA CALCIUM ION × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 6 15P POLYETHYLENE GLYCOL (N=34) × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG monomethyl ether 750, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.55 Å R-free 0.180 |
| 1Y3B Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 E60S mutant Deposited 2004-11-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：initiating Met, E60S | CA CALCIUM ION × 1 NA SODIUM ION × 1 CIT CITRIC ACID × 3 15P POLYETHYLENE GLYCOL (N=34) × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 4000, 4% acetone, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.178 |
| 1Y3B Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 E60S mutant Deposited 2004-11-24 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：initiating Met, E60S | CA CALCIUM ION × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 6 15P POLYETHYLENE GLYCOL (N=34) × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 4000, 4% acetone, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.178 |
| 1Y3C Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 R62A mutant Deposited 2004-11-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：initiating Met, R62A | CA CALCIUM ION × 1 NA SODIUM ION × 1 CIT CITRIC ACID × 3 15P POLYETHYLENE GLYCOL (N=34) × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.69 Å R-free 0.178 |
| 1Y3C Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 R62A mutant Deposited 2004-11-24 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：initiating Met, R62A | CA CALCIUM ION × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 6 15P POLYETHYLENE GLYCOL (N=34) × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.69 Å R-free 0.178 |
| 1Y3D Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 R67A mutant Deposited 2004-11-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：initiating Met, R67A | CA CALCIUM ION × 1 NA SODIUM ION × 1 CIT CITRIC ACID × 3 15P POLYETHYLENE GLYCOL (N=34) × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG monomethyl ether 750, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.173 |
| 1Y3D Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 R67A mutant Deposited 2004-11-24 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：initiating Met, R67A | CA CALCIUM ION × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 6 15P POLYETHYLENE GLYCOL (N=34) × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG monomethyl ether 750, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.173 |
| 1Y3F Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 F69A mutant Deposited 2004-11-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：initiating Met, F69A | CA CALCIUM ION × 1 NA SODIUM ION × 1 CIT CITRIC ACID × 3 15P POLYETHYLENE GLYCOL (N=34) × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 4000, 3% xylitol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.72 Å R-free 0.190 |
| 1Y3F Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 F69A mutant Deposited 2004-11-24 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：initiating Met, F69A | CA CALCIUM ION × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 6 15P POLYETHYLENE GLYCOL (N=34) × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；sodium citrate, isopropanol, PEG 4000, 3% xylitol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.72 Å R-free 0.190 |
| 1Y48 Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 R65A mutant Deposited 2004-11-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：initiating Met, R65A | CA CALCIUM ION × 1 NA SODIUM ION × 1 CIT CITRIC ACID × 3 15P POLYETHYLENE GLYCOL (N=34) × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
vapor diffusion, hanging drop, macroseeded；pH 4.6；277 K；sodium citrate, isopropanol, PEG 2000, pH 4.6, vapor diffusion, hanging drop, macroseeded, temperature 277K
|
Resolution 1.84 Å R-free 0.196 |
| 1Y48 Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 R65A mutant Deposited 2004-11-30 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：initiating Met, R65A | CA CALCIUM ION × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 6 15P POLYETHYLENE GLYCOL (N=34) × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
vapor diffusion, hanging drop, macroseeded；pH 4.6；277 K；sodium citrate, isopropanol, PEG 2000, pH 4.6, vapor diffusion, hanging drop, macroseeded, temperature 277K
|
Resolution 1.84 Å R-free 0.196 |
| 1Y4A Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59R/E60S mutant Deposited 2004-11-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：initiating Met, M59R/E60S | CA CALCIUM ION × 1 NA SODIUM ION × 1 CIT CITRIC ACID × 1 15P POLYETHYLENE GLYCOL (N=34) × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；HANGING DROP microseeded. sodium citrate, isopropanol, PEG 2000, pH 4.6, vapor diffusion, hanging drop, microseeded, temperature 277K, VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.60 Å R-free 0.203 |
| 1Y4D Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59R/E60S mutant Deposited 2004-11-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain I
22–84（63 aa）
|
Mutation：initiating Met, M59R/E60S | CA CALCIUM ION × 1 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；277 K；potassium phosphate, PEG 8000, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.252 |