当前蛋白身份：Q56229
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 4HE8 Crystal structure of the membrane domain of respiratory complex I from Thermus thermophilus 提交 2012-10-03 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 7 PDB 声明：heptameric（7） 与蛋白数一致 |
链 N
1–427（427 aa）
|
未记录 | UMQ UNDECYL-MALTOSIDE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.5；290 K；100 mM phosphate-citrate pH 4.5, 26% (v/v) PEG300, 5 mM CHAPS, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
分辨率 3.30 Å R-free 0.263 |
| 4HE8 Crystal structure of the membrane domain of respiratory complex I from Thermus thermophilus 提交 2012-10-03 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 7 PDB 声明：heptameric（7） 与蛋白数一致 |
链 I
1–427（427 aa）
|
未记录 | UMQ UNDECYL-MALTOSIDE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.5；290 K；100 mM phosphate-citrate pH 4.5, 26% (v/v) PEG300, 5 mM CHAPS, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
分辨率 3.30 Å R-free 0.263 |
| 4HEA Crystal structure of the entire respiratory complex I from Thermus thermophilus 提交 2012-10-03 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 16 PDB 声明：hexadecameric（16） 与蛋白数一致 |
链 N
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 19% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 2.2 mM FOS-CHOLINE-8 fluorinated , VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 24% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201, VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 3.30 Å R-free 0.239 |
| 4HEA Crystal structure of the entire respiratory complex I from Thermus thermophilus 提交 2012-10-03 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 16 PDB 声明：hexadecameric（16） 与蛋白数一致 |
链 V
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 19% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 2.2 mM FOS-CHOLINE-8 fluorinated , VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 24% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201, VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 3.30 Å R-free 0.239 |
| 6I0D Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone 提交 2018-10-25 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 16 PDB 声明：hexadecameric（16） 与蛋白数一致 |
链 N
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 DCQ 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201
|
分辨率 3.60 Å R-free 0.225 |
| 6I0D Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone 提交 2018-10-25 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 16 PDB 声明：hexadecameric（16） 与蛋白数一致 |
链 V
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 DCQ 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201
|
分辨率 3.60 Å R-free 0.225 |
| 6I1P Respiratory complex I from Thermus thermophilus with bound NADH 提交 2018-10-29 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 16 PDB 声明：hexadecameric（16） 与蛋白数一致 |
链 N
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside
|
分辨率 3.21 Å R-free 0.231 |
| 6I1P Respiratory complex I from Thermus thermophilus with bound NADH 提交 2018-10-29 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 16 PDB 声明：hexadecameric（16） 与蛋白数一致 |
链 V
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside
|
分辨率 3.21 Å R-free 0.231 |
| 6Q8O Respiratory complex I from Thermus thermophilus with bound Piericidin A 提交 2018-12-15 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 16 PDB 声明：hexadecameric（16） 与蛋白数一致 |
链 N
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQH Piericidin A × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside
|
分辨率 3.60 Å R-free 0.235 |
| 6Q8O Respiratory complex I from Thermus thermophilus with bound Piericidin A 提交 2018-12-15 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 16 PDB 声明：hexadecameric（16） 与蛋白数一致 |
链 V
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQH Piericidin A × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside
|
分辨率 3.60 Å R-free 0.235 |
| 6Q8W Respiratory complex I from Thermus thermophilus with bound Aureothin. 提交 2018-12-16 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 16 PDB 声明：hexadecameric（16） 与蛋白数一致 |
链 N
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQW Aureothin × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside
|
分辨率 3.40 Å R-free 0.234 |
| 6Q8W Respiratory complex I from Thermus thermophilus with bound Aureothin. 提交 2018-12-16 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 16 PDB 声明：hexadecameric（16） 与蛋白数一致 |
链 V
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQW Aureothin × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside
|
分辨率 3.40 Å R-free 0.234 |
| 6Q8X Respiratory complex I from Thermus thermophilus with bound Pyridaben. 提交 2018-12-16 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 16 PDB 声明：hexadecameric（16） 与蛋白数一致 |
链 N
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQK Pyridaben × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 21% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 1.02 mM octyl-maltoside fluorinated
|
分辨率 3.51 Å R-free 0.232 |
| 6Q8X Respiratory complex I from Thermus thermophilus with bound Pyridaben. 提交 2018-12-16 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 16 PDB 声明：hexadecameric（16） 与蛋白数一致 |
链 V
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQK Pyridaben × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 21% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 1.02 mM octyl-maltoside fluorinated
|
分辨率 3.51 Å R-free 0.232 |
| 6Y11 Respiratory complex I from Thermus thermophilus 提交 2020-02-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 16 PDB 声明：hexadecameric（16） 与蛋白数一致 |
链 N
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM
|
分辨率 3.11 Å R-free 0.229 |
| 6Y11 Respiratory complex I from Thermus thermophilus 提交 2020-02-10 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 16 PDB 声明：hexadecameric（16） 与蛋白数一致 |
链 V
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM
|
分辨率 3.11 Å R-free 0.229 |
| 6ZIY Respiratory complex I from Thermus thermophilus, NADH dataset, major state 提交 2020-06-26 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 15 PDB 声明：pentadecameric（15） 与蛋白数一致 |
链 N
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.25 Å |
| 6ZJL Respiratory complex I from Thermus thermophilus, NAD+ dataset, major state 提交 2020-06-29 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 15 PDB 声明：pentadecameric（15） 与蛋白数一致 |
链 N
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.30 Å |
| 6ZJN Respiratory complex I from Thermus thermophilus, NADH dataset, minor state 提交 2020-06-29 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 15 PDB 声明：pentadecameric（15） 与蛋白数一致 |
链 N
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 6.10 Å |
| 6ZJY Respiratory complex I from Thermus thermophilus, NAD+ dataset, minor state 提交 2020-06-29 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 15 PDB 声明：pentadecameric（15） 与蛋白数一致 |
链 N
1–427（427 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 5.50 Å |