Current Protein Identity:Q5I7K2
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 4V7E Model of the small subunit RNA based on a 5.5 A cryo-EM map of Triticum aestivum translating 80S ribosome Deposited 2013-11-22 | Assembly 1 Protein–RNA Heteromer;Protein × 82 PDB declaration: 88-meric(88) Consistent with all polymers |
Chain BH
4–192(189 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol;pH 7.5;20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol
cryo-EM vitrification conditions
Blot for 10 seconds before plunging, use 2 layers of filter paper;Cryogen ETHANE;Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT).
|
Resolution 5.50 Å |
| 8IP8 Wheat 80S ribosome stalled on AUG-Stop boron dependently Deposited 2023-03-14 | Assembly 1 Protein–RNA Heteromer;Protein × 74 PDB declaration: 81-meric(81) Consistent with all polymers |
Chain hb
1–192(192 aa)
|
Not recorded | MG MAGNESIUM ION × 307 ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8IP9 Wheat 40S ribosome in complex with a tRNAi Deposited 2023-03-14 | Assembly 1 Protein–RNA Heteromer;Protein × 32 PDB declaration: 35-meric(35) Consistent with all polymers |
Chain hb
1–192(192 aa)
|
Not recorded | MG MAGNESIUM ION × 84 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8IPA Wheat 80S ribosome stalled on AUG-Stop boron dependently with cycloheximide Deposited 2023-03-14 | Assembly 1 Protein–RNA Heteromer;Protein × 74 PDB declaration: 80-meric(80) Consistent with all polymers |
Chain hb
1–192(192 aa)
|
Not recorded | MG MAGNESIUM ION × 284 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8IPB Wheat 80S ribosome pausing on AUG-Stop with cycloheximide Deposited 2023-03-14 | Assembly 1 Protein–RNA Heteromer;Protein × 73 PDB declaration: 79-meric(79) Consistent with all polymers |
Chain hb
1–192(192 aa)
|
Not recorded | MG MAGNESIUM ION × 265 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8JIW Atomic structure of wheat ribosome reveals unique features of the plant ribosomes Deposited 2023-05-28 | Assembly 1 Protein–RNA Heteromer;Protein × 32 PDB declaration: 33-meric(33) Consistent with all polymers |
Chain BH
1–192(192 aa)
|
Not recorded | K POTASSIUM ION × 16 MG MAGNESIUM ION × 70 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.88 Å |
| 8R6F CryoEM structure of wheat 40S ribosomal subunit, body domain Deposited 2023-11-22 | Assembly 1 Protein–RNA Heteromer;Protein × 20 PDB declaration: 21-meric(21) Consistent with all polymers |
Chain H
1–192(192 aa)
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 32 K POTASSIUM ION × 14 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.34 Å |