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4v7e

Model of the small subunit RNA based on a 5.5 A cryo-EM map of Triticum aestivum translating 80S ribosome

Method: ELECTRON MICROSCOPY Dmax: 238.3 Å Quality: EXCELLENT
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1. Protein Identity and Related Structures Protein Identity & Related Structures

RACK1

OrganismNot specified

UniProt Q8LNY6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain Bg； UniProt 1–380 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain Sa 1–380（380 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q8LNY6_WHEAT
Isoform —
PDB entities 10
Chains and sequence ranges Author chain Bg； PDBConstruct 1–380； UniProt 1–380

40S ribosomal protein S3

OrganismNot specified

UniProt Q8L804

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain BD； UniProt 12–219 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain SB 12–219（208 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å
8IP8 Wheat 80S ribosome stalled on AUG-Stop boron dependently Deposited 2023-03-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 81-meric
Chain ia 1–227（227 aa）
Not recorded MG MAGNESIUM ION × 307 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8IP9 Wheat 40S ribosome in complex with a tRNAi Deposited 2023-03-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain ia 1–227（227 aa）
Not recorded MG MAGNESIUM ION × 84 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8IPA Wheat 80S ribosome stalled on AUG-Stop boron dependently with cycloheximide Deposited 2023-03-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 80-meric
Chain ia 1–227（227 aa）
Not recorded MG MAGNESIUM ION × 284 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IPB Wheat 80S ribosome pausing on AUG-Stop with cycloheximide Deposited 2023-03-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain ia 1–227（227 aa）
Not recorded MG MAGNESIUM ION × 265 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q8L804_WHEAT
Isoform —
PDB entities 11
Chains and sequence ranges Author chain BD； PDBConstruct 1–208； UniProt 12–219

40S ribosomal protein S13

OrganismNot specified

UniProt Q8L806

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain BS； UniProt 1–152 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8IP8 Wheat 80S ribosome stalled on AUG-Stop boron dependently Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 81-meric
Chain oa 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 307 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8IP9 Wheat 40S ribosome in complex with a tRNAi Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain oa 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 84 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8IPA Wheat 80S ribosome stalled on AUG-Stop boron dependently with cycloheximide Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 80-meric
Chain oa 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 284 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IPB Wheat 80S ribosome pausing on AUG-Stop with cycloheximide Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain oa 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 265 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8R57 CryoEM structure of wheat 40S ribosomal subunit, head domain Deposited 2023-11-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 15 PDB declaration: hexadecameric
Chain S 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 20 ZN ZINC ION × 2 K POTASSIUM ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.55 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q8L806_WHEAT
Isoform —
PDB entities 17
Chains and sequence ranges Author chain BS； PDBConstruct 1–152； UniProt 1–152

40S ribosomal protein S28E

OrganismNot specified

UniProt Q7X9K4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain Bc； UniProt 22–86 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8IP8 Wheat 80S ribosome stalled on AUG-Stop boron dependently Deposited 2023-03-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 81-meric
Chain ua 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 307 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8IP9 Wheat 40S ribosome in complex with a tRNAi Deposited 2023-03-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain ua 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 84 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8IPA Wheat 80S ribosome stalled on AUG-Stop boron dependently with cycloheximide Deposited 2023-03-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 80-meric
Chain ua 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 284 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IPB Wheat 80S ribosome pausing on AUG-Stop with cycloheximide Deposited 2023-03-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain ua 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 265 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q7X9K4_WHEAT
Isoform —
PDB entities 23
Chains and sequence ranges Author chain Bc； PDBConstruct 1–65； UniProt 22–86

40S ribosomal protein S14

OrganismNot specified

UniProt Q5I7K3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain Bd； UniProt 1–56 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain SN 9–56（48 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å
8R57 CryoEM structure of wheat 40S ribosomal subunit, head domain Deposited 2023-11-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 15 PDB declaration: hexadecameric
Chain d 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 20 ZN ZINC ION × 2 K POTASSIUM ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.55 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS29_WHEAT
Isoform —
PDB entities 25
Chains and sequence ranges Author chain Bd； PDBConstruct 1–56； UniProt 1–56

40S ribosomal protein S7E

OrganismNot specified

UniProt Q5I7K2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain BH； UniProt 4–192 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8IP8 Wheat 80S ribosome stalled on AUG-Stop boron dependently Deposited 2023-03-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 81-meric
Chain hb 1–192（192 aa）
Not recorded MG MAGNESIUM ION × 307 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8IP9 Wheat 40S ribosome in complex with a tRNAi Deposited 2023-03-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain hb 1–192（192 aa）
Not recorded MG MAGNESIUM ION × 84 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8IPA Wheat 80S ribosome stalled on AUG-Stop boron dependently with cycloheximide Deposited 2023-03-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 80-meric
Chain hb 1–192（192 aa）
Not recorded MG MAGNESIUM ION × 284 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IPB Wheat 80S ribosome pausing on AUG-Stop with cycloheximide Deposited 2023-03-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain hb 1–192（192 aa）
Not recorded MG MAGNESIUM ION × 265 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8JIW Atomic structure of wheat ribosome reveals unique features of the plant ribosomes Deposited 2023-05-28 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 33-meric
Chain BH 1–192（192 aa）
Not recorded K POTASSIUM ION × 16 MG MAGNESIUM ION × 70 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.88 Å
8R6F CryoEM structure of wheat 40S ribosomal subunit, body domain Deposited 2023-11-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 21-meric
Chain H 1–192（192 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 32 K POTASSIUM ION × 14 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.34 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q5I7K2_WHEAT
Isoform —
PDB entities 36
Chains and sequence ranges Author chain BH； PDBConstruct 1–189； UniProt 4–192

60S ribosomal protein L21E

OrganismNot specified

UniProt F4YWL9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain CT； UniProt 1–164 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8IP8 Wheat 80S ribosome stalled on AUG-Stop boron dependently Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 81-meric
Chain BA 1–164（164 aa）
Not recorded MG MAGNESIUM ION × 307 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8IPA Wheat 80S ribosome stalled on AUG-Stop boron dependently with cycloheximide Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 80-meric
Chain BA 1–164（164 aa）
Not recorded MG MAGNESIUM ION × 284 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IPB Wheat 80S ribosome pausing on AUG-Stop with cycloheximide Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain BA 1–164（164 aa）
Not recorded MG MAGNESIUM ION × 265 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8JIV Atomic structure of wheat ribosome reveals unique features of the plant ribosomes Deposited 2023-05-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 44-meric
Chain CT 1–164（164 aa）
Not recorded K POTASSIUM ION × 72 MG MAGNESIUM ION × 242 ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.84 Å

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F4YWL9_WHEAT
Isoform —
PDB entities 38
Chains and sequence ranges Author chain CT； PDBConstruct 1–164； UniProt 1–164

60S ribosomal protein L1

OrganismNot specified

UniProt Q5I7L3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain Cz； UniProt 1–216 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain LA 1–216（216 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q5I7L3_WHEAT
Isoform —
PDB entities 40
Chains and sequence ranges Author chain Cz； PDBConstruct 1–216； UniProt 1–216

60S ribosomal protein L5

OrganismNot specified

UniProt Q5I7L2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain CJ； UniProt 1–180 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain LE 1–170（170 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q5I7L2_WHEAT
Isoform —
PDB entities 42
Chains and sequence ranges Author chain CJ； PDBConstruct 1–180； UniProt 1–180

60S ribosomal protein L14

OrganismNot specified

UniProt Q5I7K4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain CV； UniProt 1–140 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8IP8 Wheat 80S ribosome stalled on AUG-Stop boron dependently Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 81-meric
Chain GA 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 307 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8IPA Wheat 80S ribosome stalled on AUG-Stop boron dependently with cycloheximide Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 80-meric
Chain GA 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 284 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IPB Wheat 80S ribosome pausing on AUG-Stop with cycloheximide Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain GA 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 265 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8JIV Atomic structure of wheat ribosome reveals unique features of the plant ribosomes Deposited 2023-05-28 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 44-meric
Chain CV 5–140（136 aa）
Not recorded K POTASSIUM ION × 72 MG MAGNESIUM ION × 242 ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.84 Å

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q5I7K4_WHEAT
Isoform —
PDB entities 44
Chains and sequence ranges Author chain CV； PDBConstruct 1–140； UniProt 1–140

60S ribosomal protein L18E

OrganismNot specified

UniProt Q5I7L0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain CQ； UniProt 1–188 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain LR 1–163（163 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q5I7L0_WHEAT
Isoform —
PDB entities 47
Chains and sequence ranges Author chain CQ； PDBConstruct 1–188； UniProt 1–188

60S ribosomal protein L19E

OrganismNot specified

UniProt Q7XY20

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain CR； UniProt 1–209 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain LT 1–189（189 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q7XY20_WHEAT
Isoform —
PDB entities 49
Chains and sequence ranges Author chain CR； PDBConstruct 1–209； UniProt 1–209

60S ribosomal protein L30E

OrganismNot specified

UniProt Q5I7K9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain Cc； UniProt 1–112 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1YSH Localization and dynamic behavior of ribosomal protein L30e Deposited 2005-02-08 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 3 PDB declaration: hexameric
Chain C 6–109（104 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；PLUNGED INTO ETHANE
Resolution 9.50 Å
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain Lf 1–112（112 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL30_WHEAT
Isoform —
PDB entities 55
Chains and sequence ranges Author chain Cc； PDBConstruct 1–112； UniProt 1–112

60S ribosomal protein L39E

OrganismNot specified

UniProt Q5I7K7

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain Cl； UniProt 1–51 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain Lo 1–51（51 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å
8JIV Atomic structure of wheat ribosome reveals unique features of the plant ribosomes Deposited 2023-05-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 44-meric
Chain Cl 1–51（51 aa）
Not recorded K POTASSIUM ION × 72 MG MAGNESIUM ION × 242 ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.84 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q5I7K7_WHEAT
Isoform —
PDB entities 59
Chains and sequence ranges Author chain Cl； PDBConstruct 1–51； UniProt 1–51

60S ribosomal protein L36E

OrganismNot specified

UniProt Q5I7L5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain Ci； UniProt 1–112 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain Lk 36–112（77 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å
8IP8 Wheat 80S ribosome stalled on AUG-Stop boron dependently Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 81-meric
Chain AB 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 307 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8IPA Wheat 80S ribosome stalled on AUG-Stop boron dependently with cycloheximide Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 80-meric
Chain AB 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 284 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IPB Wheat 80S ribosome pausing on AUG-Stop with cycloheximide Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain AB 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 265 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q5I7L5_WHEAT
Isoform —
PDB entities 64
Chains and sequence ranges Author chain Ci； PDBConstruct 1–112； UniProt 1–112

60S ribosomal protein P1

OrganismNot specified

UniProt Q5I7K5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain Cu； UniProt 1–110 Chain Cv； UniProt 1–110 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain Lt 6–63（58 aa）
Chain Lu 6–63（58 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q5I7K5_WHEAT
Isoform —
PDB entities 66
Chains and sequence ranges Author chain Cu； PDBConstruct 1–110； UniProt 1–110 Author chain Cv； PDBConstruct 1–110； UniProt 1–110

60S ribosomal protein L29

OrganismNot specified

UniProt Q8L805

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain Ch； UniProt 1–124 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2GO5 Structure of signal recognition particle receptor (SR) in complex with signal recognition particle (SRP) and ribosome nascent chain complex Deposited 2006-04-12 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 7 PDB declaration: nonameric
Chain 5 1–124（124 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.40 Å
2J37 MODEL OF MAMMALIAN SRP BOUND TO 80S RNCS Deposited 2006-08-18 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 6 PDB declaration: octameric
Chain 5 1–124（124 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.70 Å
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain Lc 1–124（124 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å
8IP8 Wheat 80S ribosome stalled on AUG-Stop boron dependently Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 81-meric
Chain BB 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 307 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8IPA Wheat 80S ribosome stalled on AUG-Stop boron dependently with cycloheximide Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 80-meric
Chain BB 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 284 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IPB Wheat 80S ribosome pausing on AUG-Stop with cycloheximide Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain BB 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 265 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8JIV Atomic structure of wheat ribosome reveals unique features of the plant ribosomes Deposited 2023-05-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 44-meric
Chain Ch 1–124（124 aa）
Not recorded K POTASSIUM ION × 72 MG MAGNESIUM ION × 242 ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.84 Å

7 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL35_WHEAT
Isoform —
PDB entities 68
Chains and sequence ranges Author chain Ch； PDBConstruct 1–124； UniProt 1–124

60S ribosomal protein L30

OrganismNot specified

UniProt Q5I7K6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain CF； UniProt 1–244 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain Le 1–244（244 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q5I7K6_WHEAT
Isoform —
PDB entities 69
Chains and sequence ranges Author chain CF； PDBConstruct 1–244； UniProt 1–244

60S ribosomal protein L3

OrganismNot specified

UniProt Q7X744

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain CB； UniProt 1–389 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain LC 1–389（389 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å
8JIV Atomic structure of wheat ribosome reveals unique features of the plant ribosomes Deposited 2023-05-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 44-meric
Chain CB 1–385（385 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) K POTASSIUM ION × 72 MG MAGNESIUM ION × 242 ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.84 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q7X744_WHEAT
Isoform —
PDB entities 71
Chains and sequence ranges Author chain CB； PDBConstruct 1–389； UniProt 1–389

60S ribosomal protein L13

OrganismNot specified

UniProt Q5I7L1

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain CO； UniProt 1–206 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain LK 1–206（206 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å
8IP8 Wheat 80S ribosome stalled on AUG-Stop boron dependently Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 81-meric
Chain FB 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 307 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8IPA Wheat 80S ribosome stalled on AUG-Stop boron dependently with cycloheximide Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 80-meric
Chain FB 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 284 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IPB Wheat 80S ribosome pausing on AUG-Stop with cycloheximide Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain FB 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 265 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8JIV Atomic structure of wheat ribosome reveals unique features of the plant ribosomes Deposited 2023-05-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 44-meric
Chain CO 1–206（206 aa）
Not recorded K POTASSIUM ION × 72 MG MAGNESIUM ION × 242 ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.84 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q5I7L1_WHEAT
Isoform —
PDB entities 73
Chains and sequence ranges Author chain CO； PDBConstruct 1–206； UniProt 1–206

60S ribosomal protein L43E

OrganismNot specified

UniProt D8L9P6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain Cp； UniProt 1–92 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain Lm 1–92（92 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å
8IP8 Wheat 80S ribosome stalled on AUG-Stop boron dependently Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 81-meric
Chain GB 1–92（92 aa）
Not recorded MG MAGNESIUM ION × 307 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8IPA Wheat 80S ribosome stalled on AUG-Stop boron dependently with cycloheximide Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 80-meric
Chain GB 1–92（92 aa）
Not recorded MG MAGNESIUM ION × 284 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IPB Wheat 80S ribosome pausing on AUG-Stop with cycloheximide Deposited 2023-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain GB 1–92（92 aa）
Not recorded MG MAGNESIUM ION × 265 ZN ZINC ION × 4 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8JIV Atomic structure of wheat ribosome reveals unique features of the plant ribosomes Deposited 2023-05-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 44-meric
Chain Cp 1–92（92 aa）
Not recorded K POTASSIUM ION × 72 MG MAGNESIUM ION × 242 ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.84 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name D8L9P6_WHEAT
Isoform —
PDB entities 74
Chains and sequence ranges Author chain Cp； PDBConstruct 1–92； UniProt 1–92

60S ribosomal protein L6E

OrganismNot specified

UniProt Q5I7L4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain CE； UniProt 1–219 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal protein L34E × 1 （Q5I7K8） 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain LG 1–219（219 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q5I7L4_WHEAT
Isoform —
PDB entities 79
Chains and sequence ranges Author chain CE； PDBConstruct 1–219； UniProt 1–219

60S ribosomal protein L34E

OrganismNot specified

UniProt Q5I7K8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 82 RNA 6 PDB declaration: 88-meric（88） Consistent with all polymer counts Chain Cg； UniProt 1–119 Not recorded 18S ribosomal RNA × 1 P-site tRNA × 1 5'-R(*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3' × 1 40S ribosomal protein S24E × 1 40S ribosomal protein S8E × 1 40S ribosomal protein S10E × 1 40S ribosomal protein S12E × 1 40S ribosomal protein S31e × 1 40S ribosomal protein S12 × 1 RACK1 × 1 （Q8LNY6） 40S ribosomal protein S3 × 1 （Q8L804） 40S ribosomal protein S4E × 1 40S ribosomal protein S7 × 1 40S ribosomal protein S9 × 1 40S ribosomal protein S10 × 1 40S ribosomal protein S11 × 1 40S ribosomal protein S13 × 1 （Q8L806） 40S ribosomal protein S15 × 1 40S ribosomal protein S17 × 1 40S ribosomal protein S19E × 1 40S ribosomal protein S19 × 1 40S ribosomal protein S25E × 1 40S ribosomal protein S28E × 1 （Q7X9K4） 40S ribosomal protein S8 × 1 40S ribosomal protein S14 × 1 （Q5I7K3） 40S ribosomal protein S27E × 1 40S ribosomal protein S30E × 1 40S ribosomal protein S2 × 1 40S ribosomal protein S17E × 1 40S ribosomal protein S1E × 1 40S ribosomal protein S21E × 1 40S ribosomal protein S26E × 1 40S ribosomal protein S4 × 1 40S ribosomal protein S5 × 1 40S ribosomal protein S6 × 1 40S ribosomal protein S7E × 1 （Q5I7K2） 60S ribosomal protein L8E × 1 60S ribosomal protein L21E × 1 （F4YWL9） 60S ribosomal protein L27E × 1 60S ribosomal protein L1 × 1 （Q5I7L3） 60S ribosomal protein L2 × 1 60S ribosomal protein L5 × 1 （Q5I7L2） 60S ribosomal protein L6 × 1 60S ribosomal protein L14 × 1 （Q5I7K4） 60S ribosomal protein L15E × 1 60S ribosomal protein L15 × 1 60S ribosomal protein L18E × 1 （Q5I7L0） 60S ribosomal protein L18 × 1 60S ribosomal protein L19E × 1 （Q7XY20） 60S ribosomal protein L22 × 1 60S ribosomal protein L23 × 1 60S ribosomal protein L24E × 1 60S ribosomal protein L24 × 1 60S ribosomal protein L28E × 1 60S ribosomal protein L30E × 1 （Q5I7K9） 60S ribosomal protein L31E × 1 60S ribosomal protein L32E × 1 60S ribosomal protein L37E × 1 60S ribosomal protein L39E × 1 （Q5I7K7） 60S ribosomal protein L44E × 1 60S ribosomal protein L14E × 1 60S ribosomal protein L20 × 1 60S ribosomal protein L22E × 1 60S ribosomal protein L36E × 1 （Q5I7L5） 60S ribosomal protein L11 × 1 60S ribosomal protein P1 × 2 （Q5I7K5） Acidic ribosomal protein P2 × 2 60S ribosomal protein L29 × 1 （Q8L805） 60S ribosomal protein L30 × 1 （Q5I7K6） 60S acidic ribosomal protein P0 × 1 60S ribosomal protein L3 × 1 （Q7X744） 60S ribosomal protein L4 × 1 60S ribosomal protein L13 × 1 （Q5I7L1） 60S ribosomal protein L43E × 1 （D8L9P6） 60S ribosomal protein L16 × 1 60S ribosomal protein L41E × 1 60S ribosomal protein L40E × 1 60S ribosomal protein L13E × 1 60S ribosomal protein L6E × 1 （Q5I7L4） 60S ribosomal protein L33E × 1 60S ribosomal protein L38E × 1 60S ribosomal protein L29E × 1 60S ribosomal RNA × 1 5.8S ribosomal RNA × 1 5S ribosomal RNA × 1 ELECTRON MICROSCOPY cryo-EM buffer：20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol；pH 7.5；20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol cryo-EM vitrification conditions：Blot for 10 seconds before plunging, use 2 layers of filter paper；Cryogen ETHANE；Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT). Resolution 5.50 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V7E Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 1863 PDB declaration: 2001-meric
Chain Li 1–119（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE；pH 7.6；25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
Resolution 34.00 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q5I7K8_WHEAT
Isoform —
PDB entities 83
Chains and sequence ranges Author chain Cg； PDBConstruct 1–119； UniProt 1–119

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 4v7e

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 4v7e
Download Download
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2. Structure Basics 2. Structure Basics

Entry ID entry_id4v7e
Deposition date deposition_date2013-11-22
Structure title titleModel of the small subunit RNA based on a 5.5 A cryo-EM map of Triticum aestivum translating 80S ribosome
Keywords keywordseukaryotic ribosome, homology modeling, de novo modeling, ribosomal RNA, rRNA, RNA expansion segments, RIBOSOME; RIBOSOME
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier93.33 Å
Radius of gyration Rg (electron density) rg_electron94.99 Å
Forward intensity I(0) i0276215000000.00
Molecular weight molecular_weight3131400.0 kDa
Excluded volume excluded_volume3358900 ų
Envelope volume envelope_volume5685500 ų
Hydration-shell volume shell_volume450740 ų
Envelope diameter envelope_diameter362.9 Å
Shell Rg shell_rg111.80 Å
Envelope Rg envelope_rg95.14 Å
Shape Rg shape_rg95.08 Å
Total Rg total_rg94.84 Å
Total atoms total_atoms212263
Residues n_residues17350
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax238.3 Å
Rg (real space) rg_real90.33 Å
Rg uncertainty (real space) rg_real_error0.36 Å
I(0) (real space) i0_real2.6380e+11
I(0) uncertainty (real space) i0_real_error4.3190e+09
Rg (reciprocal space) rg_reciprocal94.91 Å
I(0) (reciprocal space) i0_reciprocal277600000000.0000
Solution quality estimate total_estimate0.9129
Solution quality rating solution_quality EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary112.1 Å
Skewness Skewness skewness0.102
Kurtosis Kurtosis kurtosis-0.604
Angular range angular_range— – 0.0850 Å−1
Current regularization parameter α current_alpha0.8105
Highest regularization parameter α highest_alpha13360000000.0000
Real-space data points n_real_points18
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.001; Oscil: 0.999; Stabil: 0.965; Sysdev: 1.000; Positv: 1.000; Valcen: 0.980; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (86)

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8. Citations (3)

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9. Files and Curves (10)