Current Protein Identity:Q5I7K7
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 4V3P The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Deposited 2014-10-20 | Assembly 1 Protein–RNA Heteromer;Protein × 1863 PDB declaration: 2001-meric(2001) Consistent with all polymers |
Chain Lo
1–51(51 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE;pH 7.6;25MM HEPES-KOH, 3MM MG(OAC)2, 85MM KOAC,1.6MM DTT, 0.25MM SPERMIDINE
cryo-EM vitrification conditions
Cryogen ETHANE;VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT
MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING
WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
|
Resolution 34.00 Å |
| 4V7E Model of the small subunit RNA based on a 5.5 A cryo-EM map of Triticum aestivum translating 80S ribosome Deposited 2013-11-22 | Assembly 1 Protein–RNA Heteromer;Protein × 82 PDB declaration: 88-meric(88) Consistent with all polymers |
Chain Cl
1–51(51 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol;pH 7.5;20 mM HEPES/KOH, pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.01 mg/mL cycloheximide, 1 mM DTT, 0.01% Nikkol
cryo-EM vitrification conditions
Blot for 10 seconds before plunging, use 2 layers of filter paper;Cryogen ETHANE;Blot for 10 seconds (using 2 layers of filter paper) before plunging into liquid ethane (FEI VITROBOT).
|
Resolution 5.50 Å |
| 8JIV Atomic structure of wheat ribosome reveals unique features of the plant ribosomes Deposited 2023-05-28 | Assembly 1 Protein–RNA Heteromer;Protein × 41 PDB declaration: 44-meric(44) Consistent with all polymers |
Chain Cl
1–51(51 aa)
|
Not recorded | K POTASSIUM ION × 72 MG MAGNESIUM ION × 242 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.84 Å |