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Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：Q71SY5 New Search
10PDB entries
10Biological assembly
10chain/assembly records
Main Difference Dimensions in This Set
Different construct Different mutation/modification Different assembly state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
2KY6 Structure of ARC92VBD/MED25ACID Deposited 2010-05-14 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 391–553（163 aa） Fragment：VP16-binding-domain (VBD) Activator-interacting domain (ACID) (UNP Residues 391-553)
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 150；Pressure ambient
NMR sample composition 50-1000 uM [U-99% 15N] ARC92VBD, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition 1.0 mM ARC92VBD, 100% D2O | 100% D2O
NMR sample composition 200-1000 uM [U-99% 13C; U-99% 15N] ARC92VBD, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition 700 uM [U-100% 13C; U-100% 15N; U-80% 2H] ARC92VBD, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition 700 uM [ILV-100% 13C1H; U-100% 15N; U-100% 2H] ARC92VBD, 100% D2O | 100% D2O
Resolution not provided
2L23 NMR structure of the ACID (ACtivator Interacting Domain) of the human mediator Med25 protein Deposited 2010-08-10 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 126–283（158 aa） Fragment：ACID domain, UNP residues 391-548
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.75；293 K；Ionic strength (raw mmCIF value) 120；Pressure AMBIENT
NMR sample composition 0.3-0.5 MM [U-98% 13C U-98% 15N] MED25, 10 MM SODIUM PHOSPHATE, 100 MM SODIUM CHLORIDE, 5 MM DTT, 90% H2O/ 10% D2O | 90% H2O/10% D2O
NMR sample composition 0.3-0.5 MM [U-100% 15N] MED25, 10 MM SODIUM PHOSPHATE, 100 MM SODIUM CHLORIDE, 5 MM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 0.3-0.5 MM [U-95% 13C] MED25, 10 MM SODIUM PHOSPHATE, 100 MM SODIUM CHLORIDE, 5 MM DTT, 100% D2O | 100% D2O
NMR sample composition 0.2 MM [U-100% 15N] MED25, 10 MM SODIUM PHOSPHATE, 100 MM SODIUM CHLORIDE, 5 MM DTT, 100% D2O | 100% D2O
Resolution not provided
2L6U Solution NMR Structure of Med25(391-543) Comprising the Activator-Interacting Domain (ACID) of Human Mediator Subuniti 25. Northeast Structural Genomics Consortium Target HR6188A Deposited 2010-11-24 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 391–543（153 aa） Fragment：ACID domain residues 391-543
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Pressure AMBIENT
NMR sample composition 0.7 MM [U-100% 13C U-100% 15N] HR6188A, 20 MM MES, 100 MM SODIUM CHLORIDE, 5 MM CALCIUM CHLORIDE, 10 MM DTT, 0.02 % SODIUM AZIDE, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 0.4 MM [U-5% 13C U-100% 15N] HR6188A, 20 MM MES, 100 MM SODIUM CHLORIDE, 5 MM CALCIUM CHLORIDE, 10 MM DTT, 0.02 % SODIUM AZIDE, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2XNF The Mediator Med25 activator interaction domain: Structure and cooperative binding of VP16 subdomains Deposited 2010-08-02 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 394–543（150 aa） Fragment：MED25 ACID DOMAIN, RESIDUES 394-543
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；295 K；Ionic strength (raw mmCIF value) 100；Pressure 1.0
NMR measurement conditions pH 6.5；295 K；Ionic strength (raw mmCIF value) 100；Pressure 1.0
NMR sample composition 90%WATER/10% D2O
Resolution not provided
7EMF Human Mediator (deletion of MED1-IDR) in a Tail-extended conformation Deposited 2021-04-13 Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric（27） Consistent with protein count
Chain Y 1–747（747 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7LBM Structure of the human Mediator-bound transcription pre-initiation complex Deposited 2021-01-08 Assembly 1 Protein–DNA Heteromer；Protein × 54 PDB declaration: 56-meric（56） Consistent with all polymers
Chain 3 1–747（747 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 16 SF4 IRON/SULFUR CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
8GXQ PIC-Mediator in complex with +1 nucleosome (T40N) in MH-binding state Deposited 2022-09-21 Assembly 1 Protein–DNA Heteromer；Protein × 83 PDB declaration: 87-meric（87） Consistent with all polymers
Chain y 1–747（747 aa）
Not recorded ZN ZINC ION × 19 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.04 Å
8GXS PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state Deposited 2022-09-21 Assembly 1 Protein–DNA Heteromer；Protein × 83 PDB declaration: 87-meric（87） Consistent with all polymers
Chain y 1–747（747 aa）
Not recorded ZN ZINC ION × 19 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.16 Å
8T9D CryoEM structure of TR-TRAP Deposited 2023-06-23 Assembly 1 Protein heterocomplex Heteromer；Protein × 26 PDB declaration: 26-meric（26） Consistent with protein count
Chain T 1–747（747 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.66 Å
9CCV Crystal structure of human respiratory syncytial virus NS1 bound to human MED25 ACID Deposited 2024-06-23 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain B 389–543（155 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293.15 K；0.15 M ammonium tartrate, 0.1 M MES pH 5.6, 12% PEG 3350
Resolution 2.53 Å R-free 0.262

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.