Current Protein Identity：Q9HAU4
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1ZVD Regulation of Smurf2 Ubiquitin Ligase Activity by Anchoring the E2 to the HECT domain Deposited 2005-06-01 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
369–748（380 aa）
Fragment：HECT Domain
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | NA SODIUM ION × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；Sodium Phosphate, Potassium Phosphate, and Sodium Acetate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.10 Å R-free 0.248 |
| 2DJY Solution structure of Smurf2 WW3 domain-Smad7 PY peptide complex Deposited 2006-04-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
297–333（37 aa）
Fragment：WW3 domain
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.2；298 K；Pressure 1
|
Resolution not provided |
| 2JQZ Solution Structure of the C2 domain of human Smurf2 Deposited 2007-06-15 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
10–140（131 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.2；298 K；Ionic strength (raw mmCIF value) 0.2；Pressure ambient
NMR sample composition
20 mM HEPES, 200 mM sodium chloride, 3 mM DTT, 0.03 % sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
20 mM hepes, 200 mM sodium chloride, 3 mM DTT, 0.03 % sodium azide, 100% D2O | 100% D2O
|
Resolution not provided |
| 2KXQ Solution Structure of Smurf2 WW2 and WW3 bound to Smad7 PY motif containing peptide Deposited 2010-05-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
250–333（84 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.2；298 K；Ionic strength (raw mmCIF value) 235；Pressure ambient
NMR sample composition
1.2 mM [U-100% 13C; U-100% 15N] WW23, 1.2 mM [U-100% 13C; U-100% 15N] S7PY, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2LTZ Smurf2 WW3 domain in complex with a Smad7 derived peptide Deposited 2012-06-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
297–333（37 aa）
Fragment：WW3 domain (UNP residues 297-333)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.2；285 K；Pressure ambient
NMR sample composition
1 mM SMURF2WW3, 2 mM SMAD7, 20 mM [U-100% 2H] TRIS, 100 mM sodium chloride, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] SMURF2WW3, 3 mM SMAD7, 20 mM [U-100% 2H] TRIS, 100 mM sodium chloride, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 6FX4 Disulfide between E3 HECT ligase Smurf2 and Ubiquitin G76C Deposited 2018-03-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
631–745（115 aa）
|
Mutation：C646A, C706N, C743S | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；1.36M tri-Sodium citrat pH6.5, 15% (v/v) Glycerol
|
Resolution 2.50 Å R-free 0.239 |
| 6FX4 Disulfide between E3 HECT ligase Smurf2 and Ubiquitin G76C Deposited 2018-03-08 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
631–745（115 aa）
|
Mutation：C646A, C706N, C743S | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；1.36M tri-Sodium citrat pH6.5, 15% (v/v) Glycerol
|
Resolution 2.50 Å R-free 0.239 |
| 7M3Q Structure of the Smurf2 HECT Domain with a High Affinity Ubiquitin Variant (UbV) Deposited 2021-03-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
366–748（383 aa）
|
Not recorded | SO4 SULFATE ION × 2 NA SODIUM ION × 2 CL CHLORIDE ION × 6 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 EDO 1,2-ETHANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；1.5 M Ammonium Sulphate and 100 mM MES pH 6.0. Trypsin was added to the protein complex at a final concentration of 10 microgram per mL
|
Resolution 2.50 Å R-free 0.235 |
| 9FSH Crystal structure of the HECT domain of Smurf 2 in complex with inhibitor 8 Deposited 2024-06-21 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
251–748（498 aa）
|
Not recorded | TJQ ethyl 5-[1,3-benzodioxol-5-ylmethyl(ethyl)carbamoyl]-2,4-dimethyl-1~{H}-pyrrole-3-carboxylate × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；1.5 M NaH2PO4/K2HPO4 pH 6.8, 100 mM NaOAc
|
Resolution 2.08 Å R-free 0.259 |