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{
"code": "SASDD56",
"status": "Published",
"type_of_curve": "Single concentration",
"angular_unit": "1/nm",
"project": {
"title": "Self-Assembling Supramolecular Nanostructures Constructed from de Novo Extender Protein Nanobuilding Blocks.",
"publication": {
"title": "Self-Assembling Supramolecular Nanostructures Constructed from de Novo Extender Protein Nanobuilding Blocks.",
"author_list": "Kobayashi N, Inano K, Sasahara K, Sato T, Miyazawa K, Fukuma T, Hecht MH, Song C, Murata K, Arai R",
"journal": "ACS Synth Biol",
"doi": "10.1021/acssynbio.8b00007",
"pmid": "29690759",
"published_date": "2018 May 18"
},
"status": "released",
"submitted_date": "2018-04-12",
"released_date": "2018-05-07"
},
"pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDD56.out",
"intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDD56.dat",
"intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD56_dat_img.png",
"intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD56_kratky_img.png",
"pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDD56_pofr_img.png",
"intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD56_guinier_img.png",
"sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDD56.sascif",
"experiment": {
"instrument": {
"detector": {
"type": null,
"name": "Pilatus3 2M",
"resolution": 0.172
},
"name": "Photon Factory (PF), High Energy Accelerator Research Organization (KEK)",
"city": "Tsukuba",
"country": "Japan",
"beamline_name": "BL-10C",
"beam_geometry": null,
"type_of_source": "X-ray synchrotron",
"point_source": null,
"line_collimation": null,
"sample_path_length": null,
"line_collimation_slitlength": null,
"line_collimation_integrationwidth": null,
"xray_energy": null,
"beam_profile_ah": null,
"beam_profile_al": null
},
"sample": {
"molecule": [
{
"long_name": "stopper PN-Block (WA20)",
"short_name": "sPN-Block (WA20)",
"sequence": "MYGKLNKLVEHIKELLQQLNKNWHRHQGNLHDMNQQMEQLFQEFQHFMQGNQDDGKLQNMIHEMQQFMNQVDNHLQSESDTVHHFHNKLQELMNNFHHLVHR",
"organism": "de novo protein",
"uniprot_code": null,
"uniprot_range_first": null,
"uniprot_range_last": null,
"oligomerization": "dimer",
"molecular_type": "protein",
"uniprot_sequence": null,
"mw": 12.547,
"total_mw": 25.094,
"number_molecules": 2,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
},
{
"long_name": "extender PN-Block (FL4)",
"short_name": "ePN-Block (FL4)",
"sequence": "MYGKLNKLVEHIKELLQQLNKNWHRHQGNLHDMNQQMEQLFQEFQHFMQGNQDDGKLQNMIHEMQQFMNQVDNHLQSESDTVHHFHNKLQELMNNFHHLVHRKLSGGGGSGGGGSGGGGSGGGGSAAAMYGKLNKLVEHIKELLQQLNKNWHRHQGNLHDMNQQMEQLFQEFQHFMQGNQDDGKLQNMIHEMQQFMNQVDNHLQSESDTVHHFHNKLQELMNNFHHLVHR",
"organism": "de novo protein",
"uniprot_code": null,
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"uniprot_range_last": null,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": null,
"mw": 26.879,
"total_mw": 26.879,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
}
],
"buffer": {
"name": "20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol",
"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 7.5,
"deuteration": null
},
"purity_method": null,
"name": "Artificially designed de novo protein esPN-Block (FL4) heterocomplex, e1s2 (FL4)",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Japan",
"full_name": "Shinshu University",
"webpage": "https://www.shinshu-u.ac.jp"
}
],
"contributor_name": "Ryoichi",
"contributor_surname": "Arai",
"orcid": null
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2014-12-19",
"storage_temperature": null,
"cell_temperature": 20.0,
"exposure_time": 5.0,
"number_of_frames": 30,
"wavelength": 0.1488,
"sample_detector_distance": 1.06,
"concentration_min": null,
"concentration_max": 3.5,
"sample_volume": null,
"flow_rate": null,
"s_min": 0.112,
"s_max": 5.453,
"total_exposure_time": null,
"seccolumn": null
},
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}
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"chi_square_value": 0.0049,
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"comment": "2-state model: PDB1: e1s2FL4_Cform.pdb, Rg = 27.65 A, w1 = 0.485",
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},
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDD56_fit2_model2_img.png",
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"user": 303
}
],
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"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDD56_fit2_exAKCC7_fitfoxs_img.png",
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"chi_square_value": 0.036,
"p_value": 0.0,
"fit_residual_plot": "SASDD56_fit2_exAKCC7_fitfoxsresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDD56_fit2_exAKCC7.dat",
"fit_log": null,
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"model_mw": 51.95,
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"comment": "MultiFoXS best scoring 5-state model: PDB1, Rg = 39.984, w1 = 0.192",
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"log": "https://www.sasbdb.org/media/log_files/multifoxs_S4Zxp3f.log",
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"comment": "MultiFoXS best scoring 5-state model: PDB2, Rg = 27.7957, w2 = 0.180",
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"comment": "MultiFoXS best scoring 5-state model: PDB4, Rg = 37.2015, w4 = 0.113",
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"chi_square_value": 0.0125,
"p_value": 0.0,
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"pddf_software": "ATSAS GNOM",
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"description": null,
"experiment_description": "Synchrotron SAXS data from solutions of an artificially designed de novo protein, esPN-Block (FL4) heterocomplex e1s2 (FL4), in 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% v/v glycerol, pH 7.5 were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Acceleration Research Organization (KEK; Tsukuba, Japan) using a Pilatus 2M detector at a sample-detector distance of 1.1 m and at a wavelength of λ = 0.1488 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.50 mg/ml was measured at 20°C. 30 successive 5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
"tags": [],
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"guinier_point_first": 13,
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"intensities_log_log_plot": "SASDD56_datloglog_img.png",
"symmetry": null,
"last_modified": "2020-06-15T13:20:35.505343+02:00",
"bragg_peak": []
}
