SASDVC5

SEC-SAXS data of phosphorylated estrogen receptor alpha (pSer118), N-terminal domain (NTD) at pH 7.4

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2025-01-08T17:59:15.079200+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · SEC-SAXS data of phosphorylated estrogen receptor alpha (pSer118), N-terminal domain (NTD) at pH 7.4

Concentration— – 2.0 Buffer / pH20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEP / 7.4
Experimental temperature4.0 Facility / beamlineNational Synchrotron Light Source II (NSLS-II) / 16-ID (LiX)
Wavelength0.08189 nmExposure2.0 s × 360

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Estrogen receptor
View sequence
SNAMTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRY
proteinHomo sapiensP033721–184monomerMolecule count 120.175 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
196[3]0.01–0.25 1/AHas uncertainty columnMissing 00sasbdb/entries/c5/sasdvc5/source/SASDVC5.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)16.0—Å
i0Guinier3.83022—arbitrary
mwExperimental20.0—kDa
mwPorod43.0—kDa
porod_volumePorod70.0—ų
rgGuinier3.6350.607Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEPBuffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2024-07-17Storage / measurement temperature8.0 / 4.0
Exposure time2.0Frames360
Wavelength0.08189Sample-to-detector distance4.003
SourceX-ray synchrotronDetectorPilatus3 S 1M, Pilatus3 900 K
Facility / beamlineNational Synchrotron Light Source II (NSLS-II) / 16-ID (LiX) · Upton, NY, USA
q range0.1 – 2.5Sample volume / flow rate100.0 / 0.35

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (1 items)
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sascif:source record
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summary:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:134 field values
Field pathRaw value
codeSASDVC5
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleThe sequence–structure–function relationship of intrinsic ERα disorder
project.publication.titleThe sequence–structure–function relationship of intrinsic ERα disorder
project.publication.author_listDu Z, Wang H, Luo S, Yun Z, Wu C, Yang W, Buck M, Zheng W, Hansen A, Kao H, Yang S
project.publication.journalNature
project.publication.doi10.1038/s41586-024-08400-1
project.publication.pmidnull
project.publication.published_date2025 Jan 08
project.statusreleased
project.submitted_date2024-07-23
project.released_date2024-12-13
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pddf_plotnull
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experiment.instrument.detector.namePilatus3 S 1M, Pilatus3 900 K
experiment.instrument.detector.resolutionnull
experiment.instrument.nameNational Synchrotron Light Source II (NSLS-II)
experiment.instrument.cityUpton, NY
experiment.instrument.countryUSA
experiment.instrument.beamline_name16-ID (LiX)
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourceTrue
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
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experiment.instrument.xray_energynull
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experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameEstrogen receptor
experiment.sample.molecule[0].short_nameER-NTD
experiment.sample.molecule[0].sequenceSNAMTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRY
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP03372
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last184
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAY EFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPF LQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAK ETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQAC RLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKR SKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINW AKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEG MVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLD KITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLL LEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV
experiment.sample.molecule[0].mw20.175
experiment.sample.molecule[0].total_mw20.175
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEP
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameSEC-SAXS data of phosphorylated estrogen receptor alpha (pSer118), N-terminal domain (NTD) at pH 7.4
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressCleveland, USA
experiment.contributor[0].affiliation[0].full_nameCase Western Reserve University
experiment.contributor[0].affiliation[0].webpagehttps://case.edu/
experiment.contributor[0].contributor_nameSichun
experiment.contributor[0].contributor_surnameYang
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2024-07-17
experiment.storage_temperature8.0
experiment.cell_temperature4.0
experiment.exposure_time2.0
experiment.number_of_frames360
experiment.wavelength0.08189
experiment.sample_detector_distance4.003
experiment.concentration_minnull
experiment.concentration_max2.0
experiment.sample_volume100.0
experiment.flow_rate0.35
experiment.s_min0.1
experiment.s_max2.5
experiment.total_exposure_timenull
experiment.seccolumn54
fits[]
estimated_volume_methodnull
pddf_softwarenull
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of the phosphorylated N-terminal domain of estrogen receptor alpha (pSer118) in 20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEP, pH 7.4 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II; Upton, NY, USA) using a Pilatus3 S 1M, Pilatus3 900 K detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.08189 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 2 mg/ml was injected at a 0.35 ml/min flow rate onto a Cytiva Superdex 75 Increase 5/150 column at 4°C. 360 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
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guinier_rg_error0.607
pddf_dmax16.0
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porod_volume_errornull
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estimated_volume_errornull
guinier_point_first6
guinier_point_last30
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDVC5_datloglog_img.png
symmetrynull
last_modified2025-01-08T17:59:15.079200+01:00
bragg_peak[]
manifest.json:31 field values
Field pathRaw value
codeSASDVC5
statussuccess
started_at2026-08-11T14:45:15.525053+00:00
finished_at2026-08-11T14:45:20.800889+00:00
source_last_modified2025-01-08T17:59:15.079200+01:00
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View the complete summary.json source
{
  "code": "SASDVC5",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "The sequence–structure–function relationship of intrinsic ERα disorder",
    "publication": {
      "title": "The sequence–structure–function relationship of intrinsic ERα disorder",
      "author_list": "Du Z, Wang H, Luo S, Yun Z, Wu C, Yang W, Buck M, Zheng W, Hansen A, Kao H, Yang S",
      "journal": "Nature",
      "doi": "10.1038/s41586-024-08400-1",
      "pmid": null,
      "published_date": "2025 Jan 08"
    },
    "status": "released",
    "submitted_date": "2024-07-23",
    "released_date": "2024-12-13"
  },
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View the complete manifest.json source
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