SASDWF7

EccA5-N terminal domain and EspG5 complex of ESX-5 secretion pathway of Mycobacterium tuberculosis

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2026-02-22T17:39:35.038740+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · EccA5-N terminal domain and EspG5 complex of ESX-5 secretion pathway of Mycobacterium tuberculosis

Concentration— – 4.0 Buffer / pH50mM Hepes 200mM NaCl / 8.0
Experimental temperature10.0 Facility / beamlineCSIR-Central Drug Research Institute / Anton Paar SAXSpace
Wavelength0.154 nmExposure900.0 s × 4

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
ESX-5 secretion-associated protein EspG5
View sequence
MDQQSTRTDITVNVDGFWMLQALLDIRHVAPELRCRPYVSTDSNDWLNEHPGMAVMREQGIVVNDAVNEQVAARMKVLAAPDLEVVALLSRGKLLYGVIDDENQPPGSRDIPDNEFRVVLARRGQHWVSAVRVGNDITVDDVTVSDSASIAALVMDGLESIHHADPAAINAVNVPMEEMLEATKSWQESGFNVFSGGDLRRMGISAATVAALGQALSDPAAEVAVYARQYRDDAKGPSASVLSLKDGSGGRIALYQQARTAGSGEAWLAICPATPQLVQVGVKTVLDTLPYGEWKTHSRV
proteinMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)O53943—–—monomerMolecule count 132.4 kDa
ESX-5 secretion system protein EccA5 N-terminal domain
View sequence
GSHMTRPQAAAEDARNAMVAGLLASGISVNGLQPSHNPQVAAQMFTTATRLDPKMCDAWLARLLAGDQSIEVLAGAWAAVRTFGWETRRLGVTDLQFRPEVSDGLFLRLAITSVDSLACAYAAVLAEAKRYQEAAELLDATDPRHPFDAELVSYVRGVLYFRTKRWPDVLAQFPEATQWRHPELKAAGAAMATTALASLGVFEEAFRRAQEAIEGDRVPGAANIALYTQGMCLRHVGREEEAVELLRRVYSRDAKFTPAREALDNPNFRLILTDPETIEAR
proteinMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)P9WPI11–278monomerMolecule count 130.9 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1924[3]0.094392–6.001 1/nmHas uncertainty columnMissing 01sasbdb/entries/f7/sasdwf7/source/SASDWF7.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)10.74—nm
i0Guinier66692.4—arbitrary
i0P(r)66240.0—arbitrary
mwExperimental53.15—kDa
mwPorod55.0—kDa
porod_volumePorod84.25—nm³
rgGuinier3.3190.398nm
rgP(r)3.362—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name50mM Hepes 200mM NaClBuffer concentration—
pH8.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2025-01-28Storage / measurement temperature4.0 / 10.0
Exposure time900.0Frames4
Wavelength0.154Sample-to-detector distance0.317
SourceX-ray in houseDetectorMythen2 R 1K
Facility / beamlineCSIR-Central Drug Research Institute / Anton Paar SAXSpace · Lucknow, India
q range0.094 – 6.001Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (4 items)
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:168 field values
Field pathRaw value
codeSASDWF7
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleSAXS analysis of EccA5 N-terminal domain and EspG5 of ESX-5 secretion pathway of Mycobacterium tuberculosis
project.publicationnull
project.statusreleased
project.submitted_date2025-02-12
project.released_date2026-02-12
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDWF7.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDWF7.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWF7_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWF7_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWF7_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWF7_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDWF7.sascif
experiment.instrument.detector.typeHybrid Photon Counting (HPC)
experiment.instrument.detector.nameMythen2 R 1K
experiment.instrument.detector.resolutionnull
experiment.instrument.nameCSIR-Central Drug Research Institute
experiment.instrument.cityLucknow
experiment.instrument.countryIndia
experiment.instrument.beamline_nameAnton Paar SAXSpace
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray in house
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameESX-5 secretion-associated protein EspG5
experiment.sample.molecule[0].short_nameEspG5
experiment.sample.molecule[0].sequenceMDQQSTRTDITVNVDGFWMLQALLDIRHVAPELRCRPYVSTDSNDWLNEHPGMAVMREQGIVVNDAVNEQVAARMKVLAAPDLEVVALLSRGKLLYGVIDDENQPPGSRDIPDNEFRVVLARRGQHWVSAVRVGNDITVDDVTVSDSASIAALVMDGLESIHHADPAAINAVNVPMEEMLEATKSWQESGFNVFSGGDLRRMGISAATVAALGQALSDPAAEVAVYARQYRDDAKGPSASVLSLKDGSGGRIALYQQARTAGSGEAWLAICPATPQLVQVGVKTVLDTLPYGEWKTHSRV
experiment.sample.molecule[0].organismMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
experiment.sample.molecule[0].uniprot_codeO53943
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMDQQSTRTDITVNVDGFWMLQALLDIRHVAPELRCRPYVSTDSNDWLNEHPGMAVMREQG IVVNDAVNEQVAARMKVLAAPDLEVVALLSRGKLLYGVIDDENQPPGSRDIPDNEFRVVL ARRGQHWVSAVRVGNDITVDDVTVSDSASIAALVMDGLESIHHADPAAINAVNVPMEEML EATKSWQESGFNVFSGGDLRRMGISAATVAALGQALSDPAAEVAVYARQYRDDAKGPSAS VLSLKDGSGGRIALYQQARTAGSGEAWLAICPATPQLVQVGVKTVLDTLPYGEWKTHSRV
experiment.sample.molecule[0].mw32.4
experiment.sample.molecule[0].total_mw32.4
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.molecule[1].long_nameESX-5 secretion system protein EccA5 N-terminal domain
experiment.sample.molecule[1].short_nameEccA5NT
experiment.sample.molecule[1].sequenceGSHMTRPQAAAEDARNAMVAGLLASGISVNGLQPSHNPQVAAQMFTTATRLDPKMCDAWLARLLAGDQSIEVLAGAWAAVRTFGWETRRLGVTDLQFRPEVSDGLFLRLAITSVDSLACAYAAVLAEAKRYQEAAELLDATDPRHPFDAELVSYVRGVLYFRTKRWPDVLAQFPEATQWRHPELKAAGAAMATTALASLGVFEEAFRRAQEAIEGDRVPGAANIALYTQGMCLRHVGREEEAVELLRRVYSRDAKFTPAREALDNPNFRLILTDPETIEAR
experiment.sample.molecule[1].organismMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
experiment.sample.molecule[1].uniprot_codeP9WPI1
experiment.sample.molecule[1].uniprot_range_first1
experiment.sample.molecule[1].uniprot_range_last278
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMTRPQAAAEDARNAMVAGLLASGISVNGLQPSHNPQVAAQMFTTATRLDPKMCDAWLARL LAGDQSIEVLAGAWAAVRTFGWETRRLGVTDLQFRPEVSDGLFLRLAITSVDSLACAYAA VLAEAKRYQEAAELLDATDPRHPFDAELVSYVRGVLYFRTKRWPDVLAQFPEATQWRHPE LKAAGAAMATTALASLGVFEEAFRRAQEAIEGDRVPGAANIALYTQGMCLRHVGREEEAV ELLRRVYSRDAKFTPAREALDNPNFRLILTDPETIEARTDPWDPDSAPTRAQTEAARHAE MAAKYLAEGDAELNAMLGMEQAKKEIKLIKSTTKVNLARAKMGLPVPVTSRHTLLLGPPG TGKTSVARAFTKQLCGLTVLRKPLVVETSRTKLLGRYMADAEKNTEEMLEGALGGAVFFD EMHTLHEKGYSQGDPYGNAIINTLLLYMENHRDELVVFGAGYAKAMEKMLEVNQGLRRRF STVIEFFSYTPQELIALTQLMGRENEDVITEEESQVLLPSYTKFYMEQSYSEDGDLIRGI DLLGNAGFVRNVVEKARDHRSFRLDDEDLDAVLASDLTEFSEDQLRRFKELTREDLAEGL RAAVAEKKTK
experiment.sample.molecule[1].mw30.9
experiment.sample.molecule[1].total_mw30.9
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_description
experiment.sample.buffer.name50mM Hepes 200mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameEccA5-N terminal domain and EspG5 complex of ESX-5 secretion pathway of Mycobacterium tuberculosis
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressIndia
experiment.contributor[0].affiliation[0].full_nameCSIR - Central Drug Research Institute
experiment.contributor[0].affiliation[0].webpagehttps://cdri.res.in/
experiment.contributor[0].contributor_nameRajlakshmi
experiment.contributor[0].contributor_surnameK
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2025-01-28
experiment.storage_temperature4.0
experiment.cell_temperature10.0
experiment.exposure_time900.0
experiment.number_of_frames4
experiment.wavelength0.154
experiment.sample_detector_distance0.317
experiment.concentration_minnull
experiment.concentration_max4.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.094
experiment.s_max6.001
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modeldummy
fits[0].models[0].software_versionDAMFILT 5.0 (r13837)
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fits[0].models[0].bead_radius2.75
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fits[0].models[0].commentnull
fits[0].models[0].user1172
fits[0].fit_unit1/A
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fits[0].softwareDAMMIF
fits[0].chi_square_value0.026
fits[0].p_value0.0
fits[0].fit_residual_plotSASDWF7_fit1_fitresiduals_img.png
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fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw53.15
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw55.0
porod_mw_errornull
pddf_i066240.0
pddf_i0_errornull
guinier_i066692.4
guinier_i0_errornull
pddf_rg3.362
pddf_rg_errornull
guinier_rg3.319
guinier_rg_error0.398
pddf_dmax10.74
pddf_dmax_errornull
porod_volume84.25
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first5
guinier_point_last48
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDWF7_datloglog_img.png
symmetrynull
last_modified2026-02-22T17:39:35.038740+01:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDWF7
statussuccess
started_at2026-08-11T15:29:04.898896+00:00
finished_at2026-08-11T15:29:12.244489+00:00
source_last_modified2026-02-22T17:39:35.038740+01:00
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View the complete summary.json source
{
  "code": "SASDWF7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "SAXS analysis of EccA5 N-terminal domain and EspG5 of ESX-5 secretion pathway of Mycobacterium tuberculosis",
    "publication": null,
    "status": "released",
    "submitted_date": "2025-02-12",
    "released_date": "2026-02-12"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDWF7.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDWF7.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWF7_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWF7_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWF7_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWF7_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDWF7.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": "Hybrid Photon Counting (HPC)",
        "name": "Mythen2 R 1K",
        "resolution": null
      },
      "name": "CSIR-Central Drug Research Institute",
      "city": "Lucknow",
      "country": "India",
      "beamline_name": "Anton Paar SAXSpace",
      "beam_geometry": null,
      "type_of_source": "X-ray in house",
      "point_source": null,
      "line_collimation": null,
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      "xray_energy": null,
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      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "ESX-5 secretion-associated protein EspG5",
          "short_name": "EspG5",
          "sequence": "MDQQSTRTDITVNVDGFWMLQALLDIRHVAPELRCRPYVSTDSNDWLNEHPGMAVMREQGIVVNDAVNEQVAARMKVLAAPDLEVVALLSRGKLLYGVIDDENQPPGSRDIPDNEFRVVLARRGQHWVSAVRVGNDITVDDVTVSDSASIAALVMDGLESIHHADPAAINAVNVPMEEMLEATKSWQESGFNVFSGGDLRRMGISAATVAALGQALSDPAAEVAVYARQYRDDAKGPSASVLSLKDGSGGRIALYQQARTAGSGEAWLAICPATPQLVQVGVKTVLDTLPYGEWKTHSRV",
          "organism": "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)",
          "uniprot_code": "O53943",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
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View the complete manifest.json source
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