← Back to search
10ad

Cryo-EM structure of the human BK channel bound to the agonist NS1619

Method: ELECTRON MICROSCOPY Dmax: 151.1 Å Quality: GOOD
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Isoform 5 of Calcium-activated potassium channel subunit alpha-1

Homo sapiens

UniProt Q12791

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count Chain A； UniProt 66–1178 Chain C； UniProt 66–1178 Chain D； UniProt 66–1178 Chain E； UniProt 66–1178 Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 A1E02 3-[2-oxidanyl-5-(trifluoromethyl)phenyl]-6-(trifluoromethyl)-1~{H}-benzimidazol-2-one × 4 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 10AD Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2K44 Solution structure of a K+-channel voltage-sensor paddle domain Deposited 2008-05-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 257–284（28 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 5.5；310 K；Ionic strength (raw mmCIF value) 0.05；Pressure ambient
NMR sample composition 100mM [U-2H] 98% DPC, 50mM potassium chloride, 1mM HsapBK(233-260), 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
3NAF Structure of the Intracellular Gating Ring from the Human High-conductance Ca2+ gated K+ Channel (BK Channel) Deposited 2010-06-01 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 394–681（288 aa） Fragment：Intracellular Domain (UNP RESIDUES 329-616; 659-1113)
Chain A 786–1240（455 aa） Fragment：Intracellular Domain (UNP RESIDUES 329-616; 659-1113)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；3.5M sodium formate, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 3.10 Å R-free 0.289
6ND0 human BK channel reconstituted into liposomes Deposited 2018-12-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 292–1175（884 aa）
Chain B 292–1175（884 aa）
Chain C 292–1175（884 aa）
Chain D 292–1175（884 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE；BK proteoliposomes were applied onto a glow-discharged perforated carbon grid (CFlat R2/2, EMS), and incubated for 5-10 min In an FEI Vitrobot Mark IV (FEI). After incubation, the sample was blotted from the side and buffer containing 10 mM HEPES, pH 7.3, 75 mM KCl, and 1 mM EDTA was applied onto the TEM grid to further swell proteoliposomes. Then grids were blotted for 7 s with force 0 and fast frozen in liquid ethane cooled by liquid nitrogen.
Resolution 3.50 Å
6V22 Cryo-EM structure of Ca2+-bound hsSlo1-beta4 channel complex Deposited 2019-11-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa） Fragment：UNP residues 66-1121
Chain B 66–1121（1056 aa） Fragment：UNP residues 66-1121
Chain C 66–1121（1056 aa） Fragment：UNP residues 66-1121
Chain D 66–1121（1056 aa） Fragment：UNP residues 66-1121
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 60 CLR CHOLESTEROL × 16 K POTASSIUM ION × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.20 Å
6V35 Cryo-EM structure of Ca2+-free hsSlo1-beta4 channel complex Deposited 2019-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 40 CLR CHOLESTEROL × 8 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.50 Å
6V38 Cryo-EM structure of Ca2+-bound hsSlo1 channel Deposited 2019-11-25 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 32 CLR CHOLESTEROL × 12 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.80 Å
6V3G Cryo-EM structure of Ca2+-free hsSlo1 channel Deposited 2019-11-25 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 4.00 Å
6V5A Crystal structure of the human BK channel gating ring L390P mutant Deposited 2019-12-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 406–1121（716 aa） Fragment：Gating Ring
Mutation：L390P CA CALCIUM ION × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.9；295 K；50 mM sodium acetate, 4% (w/v) PEG 4000, 100 mM sodium sulfate, and 100 mM lithium sulfate (pH 4.9)
Resolution 2.00 Å R-free 0.226
8GH9 Cryo-EM structure of hSlo1 in total membrane vesicles Deposited 2023-03-09 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 67–1121（1055 aa）
Chain B 67–1121（1055 aa）
Chain C 67–1121（1055 aa）
Chain D 67–1121（1055 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8GHF cryo-EM structure of hSlo1 in plasma membrane vesicles Deposited 2023-03-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 67–1121（1055 aa）
Chain B 67–1121（1055 aa）
Chain C 67–1121（1055 aa）
Chain D 67–1121（1055 aa）
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 88 CLR CHOLESTEROL × 24 NA SODIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8GHG Cryo-EM structure of hSlo1 in digitonin, Ca2+-free and EDTA-free Deposited 2023-03-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 67–1121（1055 aa）
Chain B 67–1121（1055 aa）
Chain C 67–1121（1055 aa）
Chain D 67–1121（1055 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8V60 F315A mutant of human Slo1 in presence of EDTA - resting VSD Deposited 2023-12-01 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Mutation：F315A Mutation：F315A Mutation：F315A Mutation：F315A K POTASSIUM ION × 9 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 28 CLR CHOLESTEROL × 4 AJP Digitonin × 36 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.43 Å
8V63 R207A mutant of human Slo1 in presence of EDTA - activated VSD Deposited 2023-12-01 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Mutation：R207A Mutation：R207A Mutation：R207A Mutation：R207A K POTASSIUM ION × 9 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 28 CLR CHOLESTEROL × 4 AJP Digitonin × 40 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.72 Å
8V64 RCK1-RCK2 double mutant of human Slo1 in presence of EDTA - resting VSD Deposited 2023-12-01 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Mutation：D894A,D895A,D896A,D897A Mutation：D894A,D895A,D896A,D897A Mutation：D894A,D895A,D896A,D897A Mutation：D894A,D895A,D896A,D897A K POTASSIUM ION × 9 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 24 CLR CHOLESTEROL × 4 AJP Digitonin × 36 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
8VAV Human Slo1 - human LRRC26 in presence of EDTA - GR masked Deposited 2023-12-11 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 K POTASSIUM ION × 4 AJP Digitonin × 32 CLR CHOLESTEROL × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.13 Å
8VAZ Structure of human Slo1 and human LRRC26 in EDTA - LRRD masked Deposited 2023-12-11 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
8Z3S Activation mechanism and novel binding site of the BKCa channel activator CTIBD Deposited 2024-04-16 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1179（1114 aa）
Chain B 66–1179（1114 aa）
Chain C 66–1179（1114 aa）
Chain D 66–1179（1114 aa）
Not recorded A1L04 4-[4-(4-chlorophenyl)-3-(trifluoromethyl)-1,2-oxazol-5-yl]benzene-1,3-diol × 8 Y01 CHOLESTEROL HEMISUCCINATE × 4 MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
9CZH Ca2+ bound intermediate state of hSlo1 + beta2N-beta4 channel in detergent. Deposited 2024-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded CA CALCIUM ION × 8 K POTASSIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9CZJ Ca2+ free hSlo1 + beta2N-beta4 channel in detergent. Deposited 2024-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded CLR CHOLESTEROL × 4 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
9CZK Ca2+ free hSlo1 + beta2N-beta4 channel in nanodisc. Deposited 2024-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded K POTASSIUM ION × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 CLR CHOLESTEROL × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
9CZM Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in nanodisc. Deposited 2024-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 32 MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 CLR CHOLESTEROL × 8 K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.57 Å
9CZO Ca2+ bound intermediate state of hSlo1 + beta2N-beta4 channel in nanodisc. Deposited 2024-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded CA CALCIUM ION × 8 K POTASSIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 11 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.87 Å
9CZQ Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent. Deposited 2024-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.88 Å
9D18 Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent-conformation 2 of inactivating domain Deposited 2024-08-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.88 Å
9D19 Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent-conformation 3 of inactivating domain Deposited 2024-08-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.88 Å
9JO3 Cryo-EM structure of human BKca channel-compound 10b complex Deposited 2024-09-24 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1184（1119 aa）
Chain B 66–1184（1119 aa）
Chain C 66–1184（1119 aa）
Chain D 66–1184（1119 aa）
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 K POTASSIUM ION × 3 A1L4E 5-azanyl-2-[2,3,5,6-tetrakis(fluoranyl)-4-(trifluoromethyl)phenoxy]phenol × 4 Y01 CHOLESTEROL HEMISUCCINATE × 16 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9JO4 Cryo-EM structure of human BKca channel-compound 51b complex Deposited 2024-09-24 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1179（1114 aa）
Chain B 66–1179（1114 aa）
Chain C 66–1179（1114 aa）
Chain D 66–1179（1114 aa）
Not recorded A1L4F 5-(5-morpholin-4-ylpentylamino)-2-[2,3,5,6-tetrakis(fluoranyl)-4-(trifluoromethyl)phenoxy]phenol × 4 MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 K POTASSIUM ION × 3 Y01 CHOLESTEROL HEMISUCCINATE × 16 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å

27 other PDB entries and 27 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name KCMA1_HUMAN
Isoform Q12791-5
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–1113； UniProt 66–1178 Author chain C； PDBConstruct 1–1113； UniProt 66–1178 Author chain D； PDBConstruct 1–1113； UniProt 66–1178 Author chain E； PDBConstruct 1–1113； UniProt 66–1178

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 10ad

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 10ad
Download Download
▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id10ad
Deposition date deposition_date2026-01-08
Structure title titleCryo-EM structure of the human BK channel bound to the agonist NS1619
Keywords keywordsBK, Slo1, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method methodELECTRON MICROSCOPY
▼

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier49.97 Å
Radius of gyration Rg (electron density) rg_electron49.41 Å
Forward intensity I(0) i02015090000.00
Molecular weight molecular_weight398070.0 kDa
Excluded volume excluded_volume506500 ų
Envelope volume envelope_volume713620 ų
Hydration-shell volume shell_volume114590 ų
Envelope diameter envelope_diameter158.3 Å
Shell Rg shell_rg58.80 Å
Envelope Rg envelope_rg47.57 Å
Shape Rg shape_rg49.34 Å
Total Rg total_rg49.96 Å
Total atoms total_atoms55972
Residues n_residues3496
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
▼

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax151.1 Å
Rg (real space) rg_real49.66 Å
Rg uncertainty (real space) rg_real_error0.78 Å
I(0) (real space) i0_real2.0150e+09
I(0) uncertainty (real space) i0_real_error3.3750e+07
Rg (reciprocal space) rg_reciprocal50.22 Å
I(0) (reciprocal space) i0_reciprocal2017000000.0000
Solution quality estimate total_estimate0.8850
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary70.8 Å
Skewness Skewness skewness-0.062
Kurtosis Kurtosis kurtosis-0.563
Angular range angular_range— – 0.1600 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha256100000.0000
Real-space data points n_real_points33
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.893; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.947; Smooth: 0.876
▶

5. Crystallography and Experiment 5. Crystallography & Experiment

▶

6. Entities and Polymers Entities & Polymers (4)

▶

8. Citations (1)

▶

9. Files and Curves (10)